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PR00192

Identifier
FACTINCAPB  [View Relations]  [View Alignment]  
Accession
PR00192
No. of Motifs
5
Creation Date
14-JUL-1994  (UPDATE 07-JUN-1999)
Title
F-actin capping protein beta subunit signature
Database References

PROSITE; PS00231 F_ACTIN_CAPPING_BETA
BLOCKS; BL00231
PFAM; PF01115 F_actin_cap_B
INTERPRO; IPR001698
Literature References
1. MARUYAMA, K., KUROKAWA, H., OOSAWA, M., SHIMAOKA, S. YAMAMOTO, H.,
ITO, M. AND MARUYAMA, K.
Beta-actinin is equivalent to CapZ protein.
J.BIOL.CHEM. 265 8712-8715 (1990).
 
2. HARTMANN, H., NOEGEL, A.A., ECKERSKORN, C., RAPP, S. AND SCHLEICHER, M.
Ca2+-independent F-actin capping proteins. Cap 32/34, a capping protein
from Dictyolstelium discoideum, does not share sequence homologies with
known actin-binding proteins.
J.BIOL.CHEM. 264 12639-12647 (1989).

Documentation
The actin filament system, a prominent part of the cytoskeleton in 
eukaryotic cells, is both a static structure and a dynamic network that
can undergo rearrangements: it is thought to be involved in processes
such as cell movement and phagocytosis [1], as well as muscle contraction.
Capping proteins bind to the ends of actin filaments, blocking the addition
of actin monomers at this end. The capping protein is a heterodimer of alpha
and beta subunits [2]: it binds to the barbed end of actin filaments, but
unlike gelsolin and severin does not sever the filament [1]. Neither of the
subunits shows sequence similarity to other filament-capping proteins [1].
 
FACTINCAPB is a 5-element fingerprint that provides a signature for the 
F-actin capping protein beta subunit. The fingerprint was derived from
an initial alignment of 3 sequences: the motifs were drawn from conserved
regions spanning virtually the full alignment length, motif 3 including
the region encoded by PROSITE pattern F_ACTIN_CAPPING_BETA (PS00231).
Two iterations on OWL23.2 were required to reach convergence, at which
point a true set comprising 5 sequences was identified.
 
An update on SPTR37_9f identified a true set of 8 sequences.
Summary Information
8 codes involving  5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
588888
400000
300000
200000
12345
True Positives
CAPB_CAEEL    CAPB_CHICK    CAPB_DICDI    CAPB_DROME    
CAPB_HUMAN CAPB_MOUSE CAPB_YEAST P79136
Sequence Titles
CAPB_CAEEL  F-ACTIN CAPPING PROTEIN BETA SUBUNIT - CAENORHABDITIS ELEGANS. 
CAPB_CHICK F-ACTIN CAPPING PROTEIN BETA SUBUNIT ISOFORMS 1 AND 2 (CAPZ 36/32) (CAPZ B1 AND B2) (BETA-ACTININ SUBUNIT II) - GALLUS GALLUS (CHICKEN).
CAPB_DICDI F-ACTIN CAPPING PROTEIN BETA SUBUNIT (CAP32) - DICTYOSTELIUM DISCOIDEUM (SLIME MOLD).
CAPB_DROME F-ACTIN CAPPING PROTEIN BETA SUBUNIT - DROSOPHILA MELANOGASTER (FRUIT FLY).
CAPB_HUMAN F-ACTIN CAPPING PROTEIN BETA SUBUNIT (CAPZ) - HOMO SAPIENS (HUMAN).
CAPB_MOUSE F-ACTIN CAPPING PROTEIN BETA SUBUNIT ISOFORMS 1 AND 2 (CAPZ) - MUS MUSCULUS (MOUSE).
CAPB_YEAST F-ACTIN CAPPING PROTEIN BETA SUBUNIT - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
P79136 F-ACTIN CAPPING PROTEIN BETA SUBUNIT ISOFORM 3 (CAPZ) (CP BETA-3) - BOS TAURUS (BOVINE).
Scan History
OWL23_2    2  100  NSINGLE    
OWL28_0 1 80 NSINGLE
SPTR37_9f 2 9 NSINGLE
Initial Motifs
Motif 1  width=21
Element Seqn Id St Int Rpt
QLDCALDLMRRLPPQQIEKNL CAPB_CHICK 5 5 -
QLSCCLDLMRRLPPSQIEDNL CAPB_DICDI 5 5 -
QFDAALDLLRRLNPTTLQENL CAPB_YEAST 5 5 -

Motif 2 width=22
Element Seqn Id St Int Rpt
SDLIDLVPSLCEDLLSSVDQPL CAPB_CHICK 26 0 -
AGLLDLVPDLTEDLLSSIDQPL CAPB_DICDI 26 0 -
NNLIELQPNLAQDLLSSVDVPL CAPB_YEAST 26 0 -

Motif 3 width=27
Element Seqn Id St Int Rpt
KDYLLCDYNRDGDSYRSPWSNKYDPPL CAPB_CHICK 57 9 -
KDYLLCDYNRDADSYRSPWSNKYDPPL CAPB_DICDI 57 9 -
REYLCCDYNRDIDSFRSPWSNTYYPEL CAPB_YEAST 58 10 -

Motif 4 width=28
Element Seqn Id St Int Rpt
EVEANNAFDQYRDLYFEGGVSSVYLWDL CAPB_CHICK 97 13 -
EVQANEIFEIYLNLYFEGGVSSVYCWDL CAPB_DICDI 97 13 -
EILANDSFDVYRDLYYEGGISSVYLWDL CAPB_YEAST 103 18 -

Motif 5 width=27
Element Seqn Id St Int Rpt
NIGRLVEDMENKIRSTLNEIYFGKTKD CAPB_CHICK 212 87 -
NIGKMVEDMESKLRQTLETIYFGKTKE CAPB_DICDI 214 89 -
NLGSLIEDIESQMRNLLETVYFEKTRD CAPB_YEAST 229 98 -
Final Motifs
Motif 1  width=21
Element Seqn Id St Int Rpt
QLDCALDLMRRLPPQQIEKNL CAPB_CHICK 5 5 -
QLDCALDLMRRLPPQQIEKNL P79136 34 34 -
QLDCALDLMRRLPPQQIEKNL CAPB_MOUSE 5 5 -
QLDCALDLMRRLPPQQIEKNL CAPB_HUMAN 5 5 -
QMDCALDLMRRLPPQQIEKNL CAPB_DROME 5 5 -
QLDCALDLMRRLPPQHCDKNL CAPB_CAEEL 5 5 -
QLSCCLDLMRRLPPSQIEDNL CAPB_DICDI 5 5 -
QFDAALDLLRRLNPTTLQENL CAPB_YEAST 5 5 -

Motif 2 width=22
Element Seqn Id St Int Rpt
SDLIDLVPSLCEDLLSSVDQPL CAPB_CHICK 26 0 -
SDLIDLVPSLCEDLLSSVDQPL P79136 55 0 -
SDLIDLVPSLCEDLLSSVDQPL CAPB_MOUSE 26 0 -
SDLIDLVPSLCEDLLSSVDQPL CAPB_HUMAN 26 0 -
IDLIDLAPDLCEDLLSSVDQPL CAPB_DROME 26 0 -
TDLIDLCPHLVDDLLSTIDQPL CAPB_CAEEL 26 0 -
AGLLDLVPDLTEDLLSSIDQPL CAPB_DICDI 26 0 -
NNLIELQPNLAQDLLSSVDVPL CAPB_YEAST 26 0 -

Motif 3 width=27
Element Seqn Id St Int Rpt
KDYLLCDYNRDGDSYRSPWSNKYDPPL CAPB_CHICK 57 9 -
KDYLLCDYNRDGDSYRSPWSNKYDPPL P79136 86 9 -
KDYLLCDYNRDGDSYRSPWSNKYDPPL CAPB_MOUSE 57 9 -
KDYLLCDYNRDGDSYRSPWSNKYDPPL CAPB_HUMAN 57 9 -
KDYLLCDYNRDGDSYRSPWSNSYYPPL CAPB_DROME 57 9 -
KQYLLCDYNRDGDSYRSPWSNTYDPPL CAPB_CAEEL 57 9 -
KDYLLCDYNRDADSYRSPWSNKYDPPL CAPB_DICDI 57 9 -
REYLCCDYNRDIDSFRSPWSNTYYPEL CAPB_YEAST 58 10 -

Motif 4 width=28
Element Seqn Id St Int Rpt
EVEANNAFDQYRDLYFEGGVSSVYLWDL CAPB_CHICK 97 13 -
EVEANNAFDQYRDLYFEGGVSSVYLWDL P79136 126 13 -
EVEANNAFDQYRDLYFEGGVSSVYLWDL CAPB_MOUSE 97 13 -
EVEANNAFDQYRDLYFEGGVSSVYLWDL CAPB_HUMAN 97 13 -
EIEANYAFDQYREMYYEGGVSSVYLWDL CAPB_DROME 97 13 -
EIEANAAFESYRDLYFEGGVSSVYFWDL CAPB_CAEEL 97 13 -
EVQANEIFEIYLNLYFEGGVSSVYCWDL CAPB_DICDI 97 13 -
EILANDSFDVYRDLYYEGGISSVYLWDL CAPB_YEAST 103 18 -

Motif 5 width=27
Element Seqn Id St Int Rpt
NIGRLVEDMENKIRSTLNEIYFGKTKD CAPB_CHICK 212 87 -
NIGRLVEDMENKIRSTLNEIYFGKTKD P79136 241 87 -
NIGRLVEDMENKIRSTLNEIYFGKTKD CAPB_MOUSE 212 87 -
NIGRLVEDMENKIRSTLNEIYFGKTKD CAPB_HUMAN 212 87 -
NIGKMVEEMENKIRNTLNEIYFGKTKD CAPB_DROME 212 87 -
NMGRMIEDQESKMRLTINEIYFGKTKK CAPB_CAEEL 211 86 -
NIGKMVEDMESKLRQTLETIYFGKTKE CAPB_DICDI 214 89 -
NLGSLIEDIESQMRNLLETVYFEKTRD CAPB_YEAST 229 98 -