| Literature References | 1. JAP, B.K. AND WALIAN, P.J.
Biophysics of the structure and function of porins.
Q.REV.BIOPHYS. 23 367-403 (1990).
2. NIKAIDO, H.
Porins and specific channels of bacterial outer membranes.
MOL.MICROBIOL. 6 435-442 (1992).
3. WEISS, M.S., KREUSCH, A., SCHILTZ, E., NESTEL, U., WELTE, W.,
WECKESSER, J. AND SCHULZ, G.E.
The structure of porin from Rhodobacter casulatus at 1.8A resolution.
FEBS LETT. 280 379-382 (1991).
4. PAUPTIT, R.A., SCHIRMER, T., JANSONIUS, J.N., ROSENBUSCH, J.P,
PARKER, M.W., TUCKER, A.D., TSERNOGLOU, D., WIESS, M.S. AND SCHULZ, G.E.
A common channel-forming motif in evolutionarily distant porins
J.STRUCT.BIOL. 107 136-145 (1991).
5. KREUSCH, A. AND SCHULZ, G.E.
Refined structure of the porin from Rhodopseudomonas blastica.
Comparison with the porin from Rhodobacter capsulatus.
J.MOL.BIOL. 243 891-905 (1994).
6. COWAN, S.W.
Bacterial porins: lessons from three high-resolution structures.
CURR.OPIN.STRUCT.BIOL. 3 501-507 (1993).
|
| Documentation | Porins are found in the outer membranes of Gram-negative bacteria,
mitochondria and chloroplasts, where they form ion-selective channels for
small hydrophilic molecules (up to ~600 Da) [1,2]. X-ray structure analyses
of several bacterial porins [3-5] have revealed a 16-stranded anti-parallel
beta-barrel structure enclosing the transmembrane pore, by contrast with
all other integral membrane proteins described to date, which are alpha-
helical. Three subunits form a trimer; the 3-fold axis is approximately
parallel to the barrel axes and is assumed to be perpendicular to the
membrane plane [6].
From the range of porins now known, similarities have been observed between
porins from different species, and between porins of different specificity
within the same species. But most porins cannot be related to each other on
the basis of sequence alone, and this is reflected in the lengths of the
known porin sequences, which range from 282-483 residues/monomer.
ECOLIPORIN is a 6-element fingerprint that provides a signature for porins
from E.coli, Salmonella, Klebsiella and so on. The fingerprint was derived
from an initial alignment of 4 sequences: the motifs were drawn from
conserved regions spanning virtually the full alignment length. Two
iterations on OWL19.1 were required to reach convergence, at which point
a true set comprising 12 sequences was identified. The fingerprint is
selective for this particular family, and there is no cross-reaction with
porins from Neisseria or with eukaryotic porins.
An update on SPTR37_9f identified a true set of 26 sequences, and 1
partial match.
|
| Sequence Titles | NMPC_ECOLI OUTER MEMBRANE PORIN PROTEIN NMPC PRECURSOR - ESCHERICHIA COLI.
O85030 PORIN OMPN - ESCHERICHIA COLI.
O87753 OMPK35 PORIN PRECURSOR - KLEBSIELLA PNEUMONIAE.
O87754 OMPK37 PORIN PRECURSOR - KLEBSIELLA PNEUMONIAE.
OMPC_ECOLI OUTER MEMBRANE PROTEIN C PRECURSOR (PORIN OMPC) (OUTER MEMBRANE PROTEIN 1B) - ESCHERICHIA COLI.
OMPC_KLEPN OUTER MEMBRANE PROTEIN C PRECURSOR (PORIN OMPC) (PORIN OMPK36) - KLEBSIELLA PNEUMONIAE.
OMPC_SALTI OUTER MEMBRANE PROTEIN C PRECURSOR (PORIN OMPC) - SALMONELLA TYPHI.
OMPC_SALTY OUTER MEMBRANE PROTEIN C PRECURSOR (PORIN OMPC) - SALMONELLA TYPHIMURIUM.
OMPC_SERMA OUTER MEMBRANE PROTEIN C PRECURSOR (PORIN OMPC) - SERRATIA MARCESCENS.
OMPF_ECOLI OUTER MEMBRANE PROTEIN F PRECURSOR (PORIN OMPF) (OUTER MEMBRANE PROTEIN 1A, IA, OR B) - ESCHERICHIA COLI.
OMPF_SALTI OUTER MEMBRANE PROTEIN F PRECURSOR (PORIN OMPF) (OUTER MEMBRANE PROTEIN S3) - SALMONELLA TYPHI.
OMPF_SALTY OUTER MEMBRANE PROTEIN F PRECURSOR (PORIN OMPF) (OUTER MEMBRANE PROTEIN 1A, IA, OR B) - SALMONELLA TYPHIMURIUM.
OMPF_SERMA OUTER MEMBRANE PROTEIN F PRECURSOR (PORIN OMPF) - SERRATIA MARCESCENS.
OMPF_XENNE OUTER MEMBRANE PROTEIN F PRECURSOR (PORIN OMPF) (OUTER MEMBRANE PROTEIN OPNP) - XENORHABDUS NEMATOPHILUS.
OMPN_ECOLI OUTER MEMBRANE PROTEIN N PRECURSOR (PORIN OMPN) - ESCHERICHIA COLI.
OMS1_SALTI OUTER MEMBRANE PROTEIN S1 PRECURSOR - SALMONELLA TYPHI.
OMS2_SALTI OUTER MEMBRANE PROTEIN S2 PRECURSOR - SALMONELLA TYPHI.
PHOE_CITFR OUTER MEMBRANE PORE PROTEIN E PRECURSOR - CITROBACTER FREUNDII.
PHOE_ECOLI OUTER MEMBRANE PORE PROTEIN E PRECURSOR - ESCHERICHIA COLI.
PHOE_ENTCL OUTER MEMBRANE PORE PROTEIN E PRECURSOR - ENTEROBACTER CLOACAE.
PHOE_KLEOX OUTER MEMBRANE PORE PROTEIN E PRECURSOR - KLEBSIELLA OXYTOCA.
PHOE_KLEPN OUTER MEMBRANE PORE PROTEIN E PRECURSOR - KLEBSIELLA PNEUMONIAE.
PHOE_SALTI OUTER MEMBRANE PORE PROTEIN E PRECURSOR - SALMONELLA TYPHI.
PHOE_SALTY OUTER MEMBRANE PORE PROTEIN E PRECURSOR - SALMONELLA TYPHIMURIUM.
PORI_BPPA2 OUTER MEMBRANE PORIN PROTEIN LC PRECURSOR - BACTERIOPHAGE PA-2.
YEDS_ECOLI POTENTIAL OUTER MEMBRANE PROTEIN YEDS PRECURSOR - ESCHERICHIA COLI.
P77519 FROM BASES 1540626 TO 1550846 (SECTION 134 OF 400) OF THE COMPLETE GENOME (SECTION 134 OF 400) - ESCHERICHIA COLI.
|