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PR00181

Identifier
MALTOSEBP  [View Relations]  [View Alignment]  
Accession
PR00181
No. of Motifs
8
Creation Date
07-MAR-1995  (UPDATE 10-JUN-1999)
Title
Maltose binding protein signature
Database References

PROSITE; PS01037 SBP_BACTERIAL_1
INTERPRO; IPR000567
PDB; 2MBP
SCOP; 2MBP
CATH; 1DMB
Literature References
1. SHARFF, A.J., RODSETH, L.E., SZMELCMAN, S., HOFNUNG, M.
AND QUIOCHO, F.A.
Refined structures of two insertion/deletion mutants probe function
of the maltodextrin binding protein.
J.MOL.BIOL. 246 8-13 (1995).
 
2. SHARFF, A.J., RODSETH, L.E. AND QUICHO, F.A.
Refined 1.8A structure reveals the mode of binding of beta-cyclodextrin
to the maltodextrin binding protein.
BIOCHEMISTRY 32 10553-10559 (1993).
 
3. SHARFF, A.J., RODSETH, L.E., SPURLINO, J.C. AND QUICHO, F.A.
Crystallographic evidence of a large ligand-induced hinge-twist motion
between the two domains of the maltodextrin-binding protein involved
in active transport and chemotaxis.
BIOCHEMISTRY 31 10657-10663 (1992).

Documentation
Maltodextrin binding protein (MBP) is the primary component of bacterial
high-affinity active transport and chemotaxis [1]. It is a monomeric
protein, with 2 globular domains separated by a 2- or 3-stranded hinge.
MBP binds and transports linear oligosaccharides (of up to 7 glucosyl
units), as well as 2 cyclic maltodextrins which, although binding
tightly, cannot be transported nor initiate a chemotactic response [2].
It is thought that the hinge region is critical for the correct 
functioning of MBP, not just in the binding and recognition of sugars,
but also in allowing and maintaining the integrity of initiation of
both active transport and chemotaxis [3].
 
MALTOSEBP is an 8-element fingerprint that provides a signature for
maltose-binding periplasmic proteins. The fingerprint was derived from
an initial alignment of 6 sequences: the motifs were drawn from conserved
regions spanning the full alignment length. A single iteration on OWL25.2
was required to reach convergence, no further sequences being identified
beyond the starting set.
 
An update on SPTR37_9f identified a true set of 3 sequences.
Summary Information
3 codes involving  8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
833333333
700000000
600000000
500000000
400000000
300000000
200000000
12345678
True Positives
MALE_ECOLI    MALE_ENTAE    MALE_SALTY    
Sequence Titles
MALE_ECOLI  MALTOSE-BINDING PERIPLASMIC PROTEIN PRECURSOR (MALTODEXTRIN-BINDING PROTEIN) (MMBP) - ESCHERICHIA COLI. 
MALE_ENTAE MALTOSE-BINDING PERIPLASMIC PROTEIN PRECURSOR (MALTODEXTRIN-BINDING PROTEIN) (MMBP) - ENTEROBACTER AEROGENES (AEROBACTER AEROGENES).
MALE_SALTY MALTOSE-BINDING PERIPLASMIC PROTEIN PRECURSOR (MALTODEXTRIN-BINDING PROTEIN) (MMBP) - SALMONELLA TYPHIMURIUM.
Scan History
OWL25_2    1  50   NSINGLE    
SPTR37_9f 2 4 NSINGLE
Initial Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
WINGDKGYNGLAEVGKKFE MALE_ENTAE 36 36 -
WINGDKGYNGLAEVGKKFE MALE_SALTY 36 36 -
WINGDKGYNGLAEVGKKFE MALE_ECOLI 36 36 -
WINGDKGYNGLAEVGKKFE NRL_1OMP 10 10 -
WINGDKGYNGLAEVGKKFE NRL_2MBP 10 10 -
WINGDKGYNGLAEVGKKFE NRL_1DMB 10 10 -

Motif 2 width=19
Element Seqn Id St Int Rpt
EEKFPQVAATGDGPDIIFW MALE_ENTAE 70 15 -
EEKFPQVAATGDGPDIIFW MALE_SALTY 70 15 -
EEKFPQVAATGDGPDIIFW MALE_ECOLI 70 15 -
EEKFPQVAATGDGPDIIFW NRL_1OMP 44 15 -
EEKFPQVAATGDGPDIIFW NRL_2MBP 44 15 -
EEKFPQVAATGDGPDIIFW NRL_1DMB 44 15 -

Motif 3 width=20
Element Seqn Id St Int Rpt
IAYPIAVEALSLIYNKDLLP NRL_1OMP 104 41 -
IAYPIAVEALSLIYNKDLLP NRL_2MBP 104 41 -
IAYPIAVEALSLIYNKDLLP NRL_1DMB 104 41 -
IAYPIAVEALSLIYNKDLLP MALE_ECOLI 130 41 -
IAYPIAVEALSLIYNKDLVP MALE_SALTY 130 41 -
IAYPVAVEALSLIYNKDLVP MALE_ENTAE 130 41 -

Motif 4 width=20
Element Seqn Id St Int Rpt
PPKTWEEIPALDKELKAKGK MALE_ECOLI 151 1 -
PPKTWEEIPALDKALKAKGK MALE_ENTAE 151 1 -
PPKTWEEIPALDKELKVKGK MALE_SALTY 151 1 -
PPKTWEEIPALDKELKAKGK NRL_1DMB 125 1 -
PPKTWEEIPALDKELKAKGK NRL_2MBP 125 1 -
PPKTWEEIPALDKELKAKGK NRL_1OMP 125 1 -

Motif 5 width=20
Element Seqn Id St Int Rpt
SALMFNLQEPYFTWPLIAAD NRL_1DMB 145 0 -
SALMFNLQEPYFTWPLIAAD MALE_ENTAE 171 0 -
SAIMFNLQEPYFTWPLIAAD MALE_SALTY 171 0 -
SALMFNLQEPYFTWPLIAAD MALE_ECOLI 171 0 -
SALMFNLQEPYFTWPLIAAD NRL_2MBP 145 0 -
SALMFNLQEPYFTWPLIAAD NRL_1OMP 145 0 -

Motif 6 width=20
Element Seqn Id St Int Rpt
DTDYSIAEAAFNKGETAMTI NRL_1DMB 207 42 -
DTDYSIAEAAFNKGETAMTI MALE_ECOLI 233 42 -
DTDYSIAEAAFNKGETAMTI MALE_SALTY 233 42 -
DTDYSIAEAAFNKGETAMTI MALE_ENTAE 233 42 -
DTDYSIAEAAFNKGETAMTI NRL_1OMP 207 42 -
DTDYSIAEAAFNKGETAMTI NRL_2MBP 207 42 -

Motif 7 width=22
Element Seqn Id St Int Rpt
KELAKEFLENYLLTDEGLEAVN MALE_ECOLI 299 46 -
KELAKEFLENYLLTDEGLEAVN NRL_1DMB 273 46 -
KELAKEFLENYLLTDEGLEAVN NRL_2MBP 273 46 -
KELAKEFLENYLLTDQGLEAVN MALE_SALTY 299 46 -
KELAKEFLENYLMTDPGLEAVN MALE_ENTAE 299 46 -
KELAKEFLENYLLTDEGLEAVN NRL_1OMP 273 46 -

Motif 8 width=20
Element Seqn Id St Int Rpt
PNIPQMSAFWYAVRTAVINA NRL_2MBP 331 36 -
PNIPQMSAFWYAVRTAVINA MALE_SALTY 357 36 -
PNIPQMSAFWYAVRTAVINA MALE_ENTAE 357 36 -
PNIPQMSAFWYAVRTAVINA MALE_ECOLI 357 36 -
PNIPQMSAFWYAVRTAVINA NRL_1DMB 331 36 -
PNIPQMSAFWYAVRTAVINA NRL_1OMP 331 36 -
Final Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
WINGDKGYNGLAEVGKKFE MALE_ECOLI 36 36 -
WINGDKGYNGLAEVGKKFE MALE_SALTY 36 36 -
WINGDKGYNGLAEVGKKFE MALE_ENTAE 36 36 -

Motif 2 width=19
Element Seqn Id St Int Rpt
EEKFPQVAATGDGPDIIFW MALE_ECOLI 70 15 -
EEKFPQVAATGDGPDIIFW MALE_SALTY 70 15 -
EEKFPQVAATGDGPDIIFW MALE_ENTAE 70 15 -

Motif 3 width=20
Element Seqn Id St Int Rpt
IAYPIAVEALSLIYNKDLLP MALE_ECOLI 130 41 -
IAYPIAVEALSLIYNKDLVP MALE_SALTY 130 41 -
IAYPVAVEALSLIYNKDLVP MALE_ENTAE 130 41 -

Motif 4 width=20
Element Seqn Id St Int Rpt
PPKTWEEIPALDKELKAKGK MALE_ECOLI 151 1 -
PPKTWEEIPALDKELKVKGK MALE_SALTY 151 1 -
PPKTWEEIPALDKALKAKGK MALE_ENTAE 151 1 -

Motif 5 width=20
Element Seqn Id St Int Rpt
SALMFNLQEPYFTWPLIAAD MALE_ECOLI 171 0 -
SAIMFNLQEPYFTWPLIAAD MALE_SALTY 171 0 -
SALMFNLQEPYFTWPLIAAD MALE_ENTAE 171 0 -

Motif 6 width=20
Element Seqn Id St Int Rpt
DTDYSIAEAAFNKGETAMTI MALE_ECOLI 233 42 -
DTDYSIAEAAFNKGETAMTI MALE_SALTY 233 42 -
DTDYSIAEAAFNKGETAMTI MALE_ENTAE 233 42 -

Motif 7 width=22
Element Seqn Id St Int Rpt
KELAKEFLENYLLTDEGLEAVN MALE_ECOLI 299 46 -
KELAKEFLENYLLTDQGLEAVN MALE_SALTY 299 46 -
KELAKEFLENYLMTDPGLEAVN MALE_ENTAE 299 46 -

Motif 8 width=20
Element Seqn Id St Int Rpt
PNIPQMSAFWYAVRTAVINA MALE_ECOLI 357 36 -
PNIPQMSAFWYAVRTAVINA MALE_SALTY 357 36 -
PNIPQMSAFWYAVRTAVINA MALE_ENTAE 357 36 -