SPRINT Home UMBER Home Contents Standard Search Advanced Search Relation Search

==SPRINT==> PRINTS View



  selected as


PR00177

Identifier
NMDARECEPTOR  [View Relations]  [View Alignment]  
Accession
PR00177
No. of Motifs
5
Creation Date
07-JUL-1993  (UPDATE 22-JUN-1999)
Title
NMDA receptor signature
Database References

INTERPRO; IPR001508
Literature References
1. YAMAZAKI, M., MORI, H., ARAKI, K., MORI, K.J. AND MISHINA, M.
Cloning, expression, and modulation of a mouse NMDA receptor subunit.
FEBS LETT. 300(1) 39-45 (1992).
 
2. PETROU, S., ORDWAY, R.W., SINGER, J.J. AND WALSH, J.V.
A putative fatty acid-binding domain of the NMDA receptor.
TRENDS BIOCHEM.SCI. 18 41-42 (1993).

Documentation
N-Methyl-D-aspartate (NMDA) receptors are members of the glutamate receptor
channel superfamily, which mediate most of the fast excitory synaptic
transmissions in the central nervous system. The superfamily consists of 3
subtypes: kainate receptors, AMPA receptors and NMDA receptors. The NMDA
receptor is highly permeable to calcium ions and plays a key role in the
plasticity of synapses, which is believed to underlie memory and learning,
as well as the development of the nervous system. Abnormal activation has
been suggested to lead to neuronal cell death observed in many acute and
chronic disorders such as ischemia, stroke, Alzheimer's disease, and 
Huntington's disease [1].
 
NMDA receptors possess 4 transmembrane (TM) helical domains in the C-
terminal half of the sequence and require both glycine and glutamate for
activation, resulting in influx of calcium ions into the cell [1]. The
receptors can also be activated by polyamine, they can be blocked by
magnesium ions, inhibited by zinc ions, and modulated by arachidonic acid.
Other non-NMDA glutamate receptors (e.g., kainate) are structurally similar
to NMDA receptors, but are functionally distinct, possibly as a result of
amino acid substitutions at important positions in the sequence.
 
NMDARECEPTOR is a 5-element fingerprint that provides a signature for the
NMDA receptors. The fingerprint was derived from an initial alignment of 3
sequences: motif 1 encodes a conserved hydrophilic region, and motifs 2-5
encode the 4 TM helices. Two iterations on OWL20.1 were required to reach
convergence, at which point a true set comprising 5 sequences was 
identified. Several partial matches were also found, including an NMDA
receptor fragment and 11 kainate receptors, which belong to the glutamate
receptor superfamily.
 
An update on SPTR37_9f identified a true set of 10 sequences, and 19
partial matches.
Summary Information
  10 codes involving  5 elements
1 codes involving 4 elements
0 codes involving 3 elements
18 codes involving 2 elements
Composite Feature Index
51010101010
401111
300000
21026180
12345
True Positives
NMZ1_HUMAN    NMZ1_MOUSE    NMZ1_RAT      O93338        
Q24418 Q62646 Q62648 Q62683
Q91977 Q91979
True Positive Partials
Codes involving 4 elements
Q09532
Codes involving 2 elements
GLK1_DROME GLK1_HUMAN GLK1_MOUSE GLK1_RAT
GLK2_HUMAN GLK2_RAT GLK3_HUMAN GLK3_RAT
NME4_MOUSE O01623 O15399 O76903
Q18591 Q62645 Q62800 Q63381
Q63382 Q63729
Sequence Titles
NMZ1_HUMAN  GLUTAMATE [NMDA] RECEPTOR SUBUNIT ZETA 1 PRECURSOR (NR1) - HOMO SAPIENS (HUMAN). 
NMZ1_MOUSE GLUTAMATE [NMDA] RECEPTOR SUBUNIT ZETA 1 PRECURSOR (NR1) - MUS MUSCULUS (MOUSE).
NMZ1_RAT GLUTAMATE [NMDA] RECEPTOR SUBUNIT ZETA 1 PRECURSOR (NR1) (NMD-R1) (N-METHYL-D-ASPARTATE RECEPTOR) - RATTUS NORVEGICUS (RAT).
O93338 N-METHYL-D-ASPARTATE RECEPTOR NR1 SUBUNIT PRECURSOR - APTERONOTUS LEPTORHYNCHUS.
Q24418 DNMDAR-I - DROSOPHILA MELANOGASTER (FRUIT FLY).
Q62646 N-METHYL-D-ASPARTATE RECEPTOR NMDAR1-2B SUBUNIT - RATTUS NORVEGICUS (RAT).
Q62648 N-METHYL-D-ASPARTATE RECEPTOR NMDAR1-3B SUBUNIT - RATTUS NORVEGICUS (RAT).
Q62683 NMDAR1 GLUTAMATE RECEPTOR SUBUNIT - RATTUS NORVEGICUS (RAT).
Q91977 NMDA GLUTAMATE RECEPTOR SUBUNIT PRECURSOR (NMDA RECEPTOR) - XENOPUS LAEVIS (AFRICAN CLAWED FROG).
Q91979 N-METHYL-D-ASPARTATE RECEPTOR TYPE1 PRECURSOR - ANAS PLATYRHYNCHOS (DOMESTIC DUCK).

Q09532 HYPOTHETICAL 110.9 KD PROTEIN F07F6.6 IN CHROMOSOME II - CAENORHABDITIS ELEGANS.

GLK1_DROME GLUTAMATE RECEPTOR I PRECURSOR (DGLUR-I) (KAINATE-SELECTIVE GLUTAMATE RECEPTOR) - DROSOPHILA MELANOGASTER (FRUIT FLY).
GLK1_HUMAN GLUTAMATE RECEPTOR, IONOTROPIC KAINATE 1 PRECURSOR (GLUTAMATE RECEPTOR 5) (GLUR-5) (EXCITATORY AMINO ACID RECEPTOR 3) (EAA3) - HOMO SAPIENS (HUMAN).
GLK1_MOUSE GLUTAMATE RECEPTOR, IONOTROPIC KAINATE 1 PRECURSOR (GLUTAMATE RECEPTOR 5) (GLUR-5) - MUS MUSCULUS (MOUSE).
GLK1_RAT GLUTAMATE RECEPTOR, IONOTROPIC KAINATE 1 PRECURSOR (GLUTAMATE RECEPTOR 5) (GLUR-5) - RATTUS NORVEGICUS (RAT).
GLK2_HUMAN GLUTAMATE RECEPTOR, IONOTROPIC KAINATE 2 PRECURSOR (GLUTAMATE RECEPTOR 6) (GLUR-6) (EXCITATORY AMINO ACID RECEPTOR 4) (EAA4) - HOMO SAPIENS (HUMAN).
GLK2_RAT GLUTAMATE RECEPTOR, IONOTROPIC KAINATE 2 PRECURSOR (GLUTAMATE RECEPTOR 6) (GLUR-6) - RATTUS NORVEGICUS (RAT).
GLK3_HUMAN GLUTAMATE RECEPTOR, IONOTROPIC KAINATE 3 PRECURSOR (GLUTAMATE RECEPTOR 7) (GLUR-7) (EXCITATORY AMINO ACID RECEPTOR 5) (EAA5) - HOMO SAPIENS (HUMAN).
GLK3_RAT GLUTAMATE RECEPTOR, IONOTROPIC KAINATE 3 PRECURSOR (GLUTAMATE RECEPTOR 7) (GLUR-7) - RATTUS NORVEGICUS (RAT).
NME4_MOUSE GLUTAMATE [NMDA] RECEPTOR SUBUNIT EPSILON 4 PRECURSOR (N-METHYL D-ASPARTATE RECEPTOR SUBTYPE 2D) (NR2D) (NMDAR2D) - MUS MUSCULUS (MOUSE).
O01623 SIMILAR TO LIGAND-GATED IONIC CHANNEL PROTEINS - CAENORHABDITIS ELEGANS.
O15399 N-METHYL-D-ASPARTATE RECEPTOR 2D SUBUNIT PRECURSOR - HOMO SAPIENS (HUMAN).
O76903 EG:80H7.7 PROTEIN - DROSOPHILA MELANOGASTER (FRUIT FLY).
Q18591 SIMILAR TO GLUTAMATE RECEPTOR. NCBI GI: 1255330 - CAENORHABDITIS ELEGANS.
Q62645 N-METHYL-D-ASPARTATE RECEPTOR NMDAR2D SUBUNIT - RATTUS NORVEGICUS (RAT).
Q62800 IONOTROPIC GLUTAMATE RECEPTOR - RATTUS NORVEGICUS (RAT).
Q63381 NMDA RECEPTOR SUBUNIT NR2D - RATTUS NORVEGICUS (RAT).
Q63382 NMDA RECEPTOR SUBUNIT NR2D - RATTUS NORVEGICUS (RAT).
Q63729 N-METHYL-D-ASPARTATE RECEPTOR SUBUNIT - RATTUS NORVEGICUS (RAT).
Scan History
OWL20_1    2  100  NSINGLE    
OWL21_1 1 100 NSINGLE
OWL26_0 1 100 NSINGLE
SPTR37_9f 1 100 NSINGLE
Initial Motifs
Motif 1  width=29
Element Seqn Id St Int Rpt
FTYEVHLVADGKFGTQERVNNSNKKEWNG S21104 472 472 -
FTYEVHLVADGKFGTQERVNNSNKKEWNG D13515 472 472 -
FTYEVHLVADGKFGTQERVNNSNKKEWNG HUMMAR 472 472 -

Motif 2 width=26
Element Seqn Id St Int Rpt
LWLLVGLSVHVVAVMLYLLDRFSPFG S21104 562 61 -
LWLLVGLSVHVVAVMLYLLDRFSPFG D13515 562 61 -
LWLLVGLSVHVVAVMLYLLDRFSPFG HUMMAR 562 61 -

Motif 3 width=25
Element Seqn Id St Int Rpt
ALTLSSAMWFSWGVLLNSGIGEGAP S21104 600 12 -
ALTLSSAMWFSWGVLLNSGIGEGAP D13515 600 12 -
ALTLSSAMWFSWGVLLNSGIGEGAP HUMMAR 600 12 -

Motif 4 width=28
Element Seqn Id St Int Rpt
ILGMVWAGFAMIIVASYTANLAAFLVLD S21104 631 6 -
ILGMVWAGFAMIIVASYTANLAAFLVLD D13515 631 6 -
ILGMVWAGFAMIIVASYTANLAAFLVLD HUMMAR 631 6 -

Motif 5 width=25
Element Seqn Id St Int Rpt
MAGVFMLVAGGIVAGIFLIFIEIAY S21104 813 154 -
MAGVFMLVAGGIVAGIFLIFIEIAY D13515 813 154 -
MAGVFMLVAGGIVAGIFLIFIEIAY HUMMAR 813 154 -
Final Motifs
Motif 1  width=29
Element Seqn Id St Int Rpt
FTYEVHLVADGKFGTQERVNNSNKKEWNG NMZ1_HUMAN 472 472 -
FTYEVHLVADGKFGTQERVNNSNKKEWNG Q91979 470 470 -
FTYEVHLVADGKFGTQERVNNSNKKEWNG Q91977 491 491 -
FTYEVHLVADGKFGTQERVNNSNKKEWNG Q62683 472 472 -
FTYEVHLVADGKFGTQERVNNSNKKEWNG Q62648 493 493 -
FTYEVHLVADGKFGTQERVNNSNKKEWNG Q62646 493 493 -
FTYEVHLVADGKFGTQERVNNSNKKEWNG NMZ1_RAT 472 472 -
FTYEVHLVADGKFGTQERVNNSNKKEWNG NMZ1_MOUSE 472 472 -
FTYEVHLVADGKFGTQERVNNSNKKEWNG O93338 488 488 -
FTYDLALSPDGQFGHYILRNNTGAMTLRK Q24418 482 482 -

Motif 2 width=26
Element Seqn Id St Int Rpt
LWLLVGLSVHVVAVMLYLLDRFSPFG NMZ1_HUMAN 562 61 -
LWLLVGLSVHVVAVMLYLLDRFSPFG Q91979 560 61 -
LWLLVGLSVHVVAVMLYLLDRFSPFG Q91977 581 61 -
LWLLVGLSVHVVAVMLYLLDRFSPFG Q62683 562 61 -
LWLLVGLSVHVVAVMLYLLDRFSPFG Q62648 583 61 -
LWLLVGLSVHVVAVMLYLLDRFSPFG Q62646 583 61 -
LWLLVGLSVHVVAVMLYLLDRFSPFG NMZ1_RAT 562 61 -
LWLLVGLSVHVVAVMLYLLDRFSPFG NMZ1_MOUSE 562 61 -
LWLLVGLSVHVVAVMLYLLDRFSPFG O93338 578 61 -
LWILVMVSVHVVALVLYLLDRFSPFG Q24418 576 65 -

Motif 3 width=25
Element Seqn Id St Int Rpt
ALTLSSAMWFSWGVLLNSGIGEGAP NMZ1_HUMAN 600 12 -
ALTLSSAMWFSWGVLLNSGIGEGAP Q91979 598 12 -
ALTLSSAMWFSWGVLLNSGIGEGAP Q91977 619 12 -
ALTLSSAMWFSWGVLLNSGIGEGAP Q62683 600 12 -
ALTLSSAMWFSWGVLLNSGIGEGAP Q62648 621 12 -
ALTLSSAMWFSWGVLLNSGIGEGAP Q62646 621 12 -
ALTLSSAMWFSWGVLLNSGIGEGAP NMZ1_RAT 600 12 -
ALTLSSAMWFSWGVLLNSGIGEGAP NMZ1_MOUSE 600 12 -
ALTLSSAMWFSWGVLLNSGIGEGAP O93338 616 12 -
ALNLSSAVWFAWGVLLNSGIGEGTP Q24418 615 13 -

Motif 4 width=28
Element Seqn Id St Int Rpt
ILGMVWAGFAMIIVASYTANLAAFLVLD NMZ1_HUMAN 631 6 -
ILGMVWAGFAMIIVASYTANLAAFLVLD Q91979 629 6 -
ILGMVWAGFAMIIVASYTANLAAFLVLD Q91977 650 6 -
ILGMVWAGFAMIIVASYTANLAAFLVLD Q62683 631 6 -
ILGMVWAGFAMIIVASYTANLAAFLVLD Q62648 652 6 -
ILGMVWAGFAMIIVASYTANLAAFLVLD Q62646 652 6 -
ILGMVWAGFAMIIVASYTANLAAFLVLD NMZ1_RAT 631 6 -
ILGMVWAGFAMIIVASYTANLAAFLVLD NMZ1_MOUSE 631 6 -
ILGMVWAGFAMIIVASYTANLAAFLVLD O93338 647 6 -
VLGMVWAGFAMIIVASYTANLAAFLVLE Q24418 646 6 -

Motif 5 width=25
Element Seqn Id St Int Rpt
MAGVFMLVAGGIVAGIFLIFIEIAY NMZ1_HUMAN 813 154 -
MAGVFMLVAGGIVAGIFLIFIEIAY Q91979 811 154 -
MAGVFMLVAGGIVAGIFLIFIEIAY Q91977 832 154 -
MAGVFMLVAGGIVAGIFLIFIEIAY Q62683 813 154 -
MAGVFMLVAGGIVAGIFLIFIEIAY Q62648 834 154 -
MAGVFMLVAGGIVAGIFLIFIEIAY Q62646 834 154 -
MAGVFMLVAGGIVAGIFLIFIEIAY NMZ1_RAT 813 154 -
MAGVFMLVAGGIVAGIFLIFIEIAY NMZ1_MOUSE 813 154 -
MAGVFMLVAGGIAAGIFLIFIEIAY O93338 829 154 -
MAGVFILVGVGIAGGVGLIIIEVIY Q24418 832 158 -