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PR00132

Identifier
GLHYDRLASE2  [View Relations]  [View Alignment]  
Accession
PR00132
No. of Motifs
5
Creation Date
08-NOV-1994  (UPDATE 07-JUN-1999)
Title
Glycosyl hydrolase family 2 signature
Database References

PROSITE; PS00719 GLYCOSYL_HYDROL_F2_1; PS00608 GLYCOSYL_HYDROLASE_F2_2
BLOCKS; BL00719
INTERPRO; IPR001649
Literature References
1. HENRISSAT, B. AND BAIROCH, A.
New families in the classification of glycosyl hydrolases based on amino
acid sequence similarities.
BIOCHEM.J. 293 781-788 (1993).
 
2. HENRISSAT, B.
A classification of glycosyl hydrolases based on amino acid sequence
similarities.
BIOCHEM.J. 280 309-316 (1991).
 
3. SCHROEDER, C.J., ROBERT, C., LENZEN, G., MCKAY, L.L. AND MERCENIER, A.
Analysis of the lacZ sequences from 2 Streptococcus thermophilus strains -
Comparison with the Escherichia coli and Lactobacillus bulgaricus beta-
galactosidase sequences.
J.GEN.MICROBIOL. 137 369-380 (1991).
 
4. GEBLER, J.C., AEBERSOLD, R. AND WITHERS, S.G.
Glu-537, not Glu-461, is the nucleophile in the active site of (lacZ)
beta-galactosidase from Escherichia coli.
J.BIOL.CHEM. 267 11126-11130 (1992). 

Documentation
O-Glycosyl hydrolases (EC 3.2.1.-) are a widespread group of enzymes that
hydrolyse the glycosidic bond between two or more carbohydrates, or between
a carbohydrate and a non-carbohydrate moiety. A classification system for
glycosyl hydrolases, based on sequence similarity, has led to the definition
of up to 60 different families [1-3] (http://expasy.hcuge.ch/cgi-bin/lists?
glycosid.txt).
 
Family 2 includes various bacterial beta-galactosidases and beta-
glucuronidases. Alignments of such sequences reveal a number of short
conserved regions, one of which contains a glutamic acid residue, which has
been shown to be the general acid/base catalyst in the enzyme active site
in E.coli lacZ [4].
 
GLHYDRLASE2 is a 5-element fingerprint that provides a signature for
family 2 glycosyl hydrolases. The fingerprint was derived from an initial
alignment of 5 sequences: the motifs were drawn from conserved regions 
spanning virtually the full alignment length - motifs 3 and 4 include 
regions encoded by PROSITE patterns GLYCOSYL_HYDROLASE_F2_1 (PS00719)
GLYCOSYL-HYDROLASE_F2_2 (PS00608), the latter containing the catalytically
active Glu. Two iterations on OWL24.0 were required to reach convergence,
at which point a true set comprising 23 sequences was identified. Four
partial matches were also found: BGAL_THETU fails to make a significant
match with motif 5; RHMLACZ only matches motifs 2 and 3; BGLB_BACPO is a
family 1 glycosyl hydrolase that matches motifs 3 and 5; and GUNA_XANCP is
a family 5 glycosyl hydrolase that matches motifs 3 and 4.
 
An update on SPTR37_9f identified a true set of 29 sequences, and 3
partial matches.
Summary Information
  29 codes involving  5 elements
2 codes involving 4 elements
0 codes involving 3 elements
1 codes involving 2 elements
Composite Feature Index
52929292929
422220
300000
201100
12345
True Positives
BGA2_ECOLI    BGAL_ACTPL    BGAL_ARTSP    BGAL_BACME    
BGAL_CLOAB BGAL_ECOLI BGAL_ENTCL BGAL_KLEPN
BGAL_KLULA BGAL_LACAC BGAL_LACDE BGAL_LACLA
BGAL_LACSK BGAL_LEULA BGAL_STAXY BGAL_STRTR
BGAL_THEMA BGLR_CANFA BGLR_ECOLI BGLR_HUMAN
BGLR_MOUSE BGLR_RAT O09266 O09267
O85167 O85250 O87523 P97096
Q47170
True Positive Partials
Codes involving 4 elements
BGAL_THEET BGAL_THETU
Codes involving 2 elements
BGAL_RHIME
Sequence Titles
BGA2_ECOLI  EVOLVED BETA-GALACTOSIDASE ALPHA-SUBUNIT (EC 3.2.1.23) (LACTASE) - ESCHERICHIA COLI. 
BGAL_ACTPL BETA-GALACTOSIDASE (EC 3.2.1.23) (LACTASE) - ACTINOBACILLUS PLEUROPNEUMONIAE (HAEMOPHILUS PLEUROPNEUMONIAE).
BGAL_ARTSP BETA-GALACTOSIDASE (EC 3.2.1.23) (LACTASE) - ARTHROBACTER SP. (STRAIN B7).
BGAL_BACME BETA-GALACTOSIDASE (EC 3.2.1.23) (LACTASE) - BACILLUS MEGATERIUM.
BGAL_CLOAB BETA-GALACTOSIDASE (EC 3.2.1.23) (LACTASE) - CLOSTRIDIUM ACETOBUTYLICUM.
BGAL_ECOLI BETA-GALACTOSIDASE (EC 3.2.1.23) (LACTASE) - ESCHERICHIA COLI.
BGAL_ENTCL BETA-GALACTOSIDASE (EC 3.2.1.23) (LACTASE) - ENTEROBACTER CLOACAE.
BGAL_KLEPN BETA-GALACTOSIDASE (EC 3.2.1.23) (LACTASE) - KLEBSIELLA PNEUMONIAE.
BGAL_KLULA BETA-GALACTOSIDASE (EC 3.2.1.23) (LACTASE) - KLUYVEROMYCES LACTIS (YEAST).
BGAL_LACAC BETA-GALACTOSIDASE LARGE SUBUNIT (EC 3.2.1.23) (LACTASE) - LACTOBACILLUS ACIDOPHILUS.
BGAL_LACDE BETA-GALACTOSIDASE (EC 3.2.1.23) (LACTASE) - LACTOBACILLUS DELBRUECKII (SUBSP. BULGARICUS).
BGAL_LACLA BETA-GALACTOSIDASE (EC 3.2.1.23) (LACTASE) - LACTOCOCCUS LACTIS (SUBSP. LACTIS) (STREPTOCOCCUS LACTIS).
BGAL_LACSK BETA-GALACTOSIDASE LARGE SUBUNIT (EC 3.2.1.23) (LACTASE) - LACTOBACILLUS SAKE.
BGAL_LEULA BETA-GALACTOSIDASE LARGE SUBUNIT (EC 3.2.1.23) (LACTASE) - LEUCONOSTOC LACTIS.
BGAL_STAXY BETA-GALACTOSIDASE (EC 3.2.1.23) (LACTASE) - STAPHYLOCOCCUS XYLOSUS.
BGAL_STRTR BETA-GALACTOSIDASE (EC 3.2.1.23) (LACTASE) - STREPTOCOCCUS THERMOPHILUS.
BGAL_THEMA BETA-GALACTOSIDASE (EC 3.2.1.23) (LACTASE) - THERMOTOGA MARITIMA.
BGLR_CANFA BETA-GLUCURONIDASE PRECURSOR (EC 3.2.1.31) - CANIS FAMILIARIS (DOG).
BGLR_ECOLI BETA-GLUCURONIDASE (EC 3.2.1.31) (GUS) (BETA-D-GLUCURONOSIDE GLUCURONOSOHYDROLASE) - ESCHERICHIA COLI.
BGLR_HUMAN BETA-GLUCURONIDASE PRECURSOR (EC 3.2.1.31) (BETA-G1) - HOMO SAPIENS (HUMAN).
BGLR_MOUSE BETA-GLUCURONIDASE PRECURSOR (EC 3.2.1.31) - MUS MUSCULUS (MOUSE).
BGLR_RAT BETA-GLUCURONIDASE PRECURSOR (EC 3.2.1.31) - RATTUS NORVEGICUS (RAT).
O09266 BETA-GALACTOSIDASE - UNIDENTIFIED.
O09267 BETA-GALACTOSIDASE - UNIDENTIFIED.
O85167 BETA-GALACTOSIDASE - BACILLUS MEGATERIUM.
O85250 BETA-GALACTOSIDASE - THERMOTOGA NEAPOLITANA.
O87523 BETA-GALACTOSIDASE - LACTOCOCCUS LACTIS.
P97096 CLONING VECTOR PLACZI, COMPLETE PLASMID SEQUENCE - CLONING VECTOR PLACZI.
Q47170 EVOLVED BETA-GALACTOSIDASE - ESCHERICHIA COLI.

BGAL_THEET BETA-GALACTOSIDASE (EC 3.2.1.23) (LACTASE) - THERMOANAEROBACTER ETHANOLICUS (CLOSTRIDIUM THERMOHYDROSULFURICUM).
BGAL_THETU BETA-GALACTOSIDASE (EC 3.2.1.23) (LACTASE) - THERMOANAEROBACTER THERMOSULFUROGENES (CLOSTRIDIUM THERMOSULFUROGENES).

BGAL_RHIME BETA-GALACTOSIDASE (EC 3.2.1.23) (LACTASE) - RHIZOBIUM MELILOTI.
Scan History
OWL24_0    2  100  NSINGLE    
SPTR37_9f 2 106 NSINGLE
Initial Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
VNSAFHLWCNGRWVGY BGAL_ECOLI 146 146 -
VNSAFHLWCNGVWVGY BGAL_KLEPN 152 152 -
VEEALYVWLNGHFIGY BGAL_LEULA 154 154 -
AHYYAVVWVNGIHVVE BGLR_RAT 126 126 -
VTHYGKVWVNNQEVME BGLR_ECOLI 91 91 -

Motif 2 width=15
Element Seqn Id St Int Rpt
LNGKPLLIRGVNRHE BGAL_ECOLI 344 182 -
LNGKPLLIRGVNRHE BGAL_KLEPN 351 183 -
VNNKRLVINGVNRHE BGAL_LEULA 341 171 -
INGKPFYFQGVNKHE BGLR_RAT 334 192 -
INHKPFYFTGFGRHE BGLR_ECOLI 283 176 -

Motif 3 width=19
Element Seqn Id St Int Rpt
DILLMKQNNFNAVRCSHYP BGAL_ECOLI 375 16 -
DILLMKQNNFNAVRCSHYP BGAL_KLEPN 382 16 -
DIQTMLANNINADRTCHYP BGAL_LEULA 372 16 -
DFNLLRWLGANSFRTSHYP BGLR_RAT 365 16 -
DHALMDWIGANSYRTSHYP BGLR_ECOLI 314 16 -

Motif 4 width=16
Element Seqn Id St Int Rpt
DRNHPSVIIWSLGNES BGAL_ECOLI 447 53 -
NRNHPCIIIWSLGNES BGAL_KLEPN 454 53 -
FKNHPSIIFWSLGNES BGAL_LEULA 452 61 -
DKNHPAVVMWSVANEP BGLR_RAT 433 49 -
DKNHPSVVMWSIANEP BGLR_ECOLI 399 66 -

Motif 5 width=16
Element Seqn Id St Int Rpt
RPLILCEYAHAMGNSL BGAL_ECOLI 531 68 -
RPLILCEYAHAMGNSL BGAL_KLEPN 538 68 -
KPFLNCEYMHDMGNSL BGAL_LEULA 528 60 -
KPIIQSEYGADAVSGL BGLR_RAT 530 81 -
QPIIITEYGVDTLAGL BGLR_ECOLI 497 82 -
Final Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
VESAFYVWINGEFVGY BGAL_BACME 159 159 -
VNSAFHLWCNGRWVGY BGAL_ECOLI 146 146 -
VNSAFHLWCNGRWVGY P97096 168 168 -
VNSAFHLWCNGRWVGY O09266 153 153 -
VESAFYVWINGEFVGY O85167 159 159 -
VNSAFHLWCNGVWVGY BGAL_KLEPN 152 152 -
VNSAFHLWCNGQWIGY BGAL_ENTCL 148 148 -
VNLAFHLWCNVRWVGY O09267 158 158 -
VGSAFHFWLNGEYGGY BGAL_LACLA 134 134 -
VGSAFHFWLNGEYGGY O87523 132 132 -
VRSFFYLWVNGKKIGF BGAL_THEMA 136 136 -
VRSFFYLWVNGKRMGF O85250 136 136 -
VATSIFVWVNGNFVGY BGAL_STRTR 146 146 -
VETAFYVWVNGEFVGY BGAL_CLOAB 147 147 -
VEQAMYVWLNGQFIGY BGAL_LACSK 155 155 -
AATAIYVWLNGHFVGY BGAL_LACDE 151 151 -
VEEALYVWLNGHFIGY BGAL_LEULA 154 154 -
VDNCYELYVNGQYVGF BGAL_KLULA 132 132 -
VETYFEVYVNGQYVGF BGA2_ECOLI 135 135 -
VETYFEVYVNGQYVGF Q47170 68 68 -
VESRYKVWVNGVEIGV BGAL_ARTSP 143 143 -
AERAMYVWLNGHFIGY BGAL_LACAC 155 155 -
VDSAFYVWINNEFIGY BGAL_STAXY 136 136 -
VDSCLFVYVNKQFVGY BGAL_ACTPL 134 134 -
AHYYAIVWVNGVHVAE BGLR_CANFA 126 126 -
AHSYAIVWVNGVDTLE BGLR_HUMAN 126 126 -
AHYYAVVWVNGIHVVE BGLR_RAT 126 126 -
AHYYAVVWVNGIHVVE BGLR_MOUSE 126 126 -
VTHYGKVWVNNQEVME BGLR_ECOLI 91 91 -

Motif 2 width=15
Element Seqn Id St Int Rpt
INGKRIVLRGVNRHE BGAL_BACME 356 181 -
LNGKPLLIRGVNRHE BGAL_ECOLI 344 182 -
LNGKPLLIRGVNRHE P97096 366 182 -
LNGKPLLIRGVNRHE O09266 351 182 -
INGKRIVLRGVNRHE O85167 356 181 -
LNGKPLLIRGVNRHE BGAL_KLEPN 351 183 -
LNGKPLLIRGVNRHE BGAL_ENTCL 346 182 -
LNGKPLLIRGVNRHE O09267 356 182 -
INGKALLVRGVNKHE BGAL_LACLA 314 164 -
INGKALLVRGVNKHE O87523 312 164 -
FNGKPLYIKGVNRHE BGAL_THEMA 322 170 -
FNGKPLYIKGVNRHE O85250 322 170 -
LNGKRIVFKGVNRHE BGAL_STRTR 333 171 -
LKWKRIIFKGVNRHE BGAL_CLOAB 334 171 -
LNGKRLVINGVNRHE BGAL_LACSK 340 169 -
LNGQRIVFKGANRHE BGAL_LACDE 338 171 -
VNNKRLVINGVNRHE BGAL_LEULA 341 171 -
VNGKDILFRGVNRHD BGAL_KLULA 342 194 -
INNRYVMLHGVNRHD BGA2_ECOLI 329 178 -
INNRYVMLHGVNRHD Q47170 262 178 -
VNGRKVIFHGVNRHE BGAL_ARTSP 314 155 -
LNGKCLIINGVNRHE BGAL_LACAC 343 172 -
INGQSIKIRGTNYHD BGAL_STAXY 311 159 -
FNQQPIKFKGVNRHD BGAL_ACTPL 308 158 -
INGKPFYFHGVNKHE BGLR_CANFA 337 195 -
INGKPFYFHGVNKHE BGLR_HUMAN 338 196 -
INGKPFYFQGVNKHE BGLR_RAT 334 192 -
INGKPFYFQGVNKHE BGLR_MOUSE 334 192 -
INHKPFYFTGFGRHE BGLR_ECOLI 283 176 -

Motif 3 width=19
Element Seqn Id St Int Rpt
DILLMKQHNINAVRTSHYP BGAL_BACME 388 17 -
DILLMKQNNFNAVRCSHYP BGAL_ECOLI 375 16 -
DILLMKQNNFNAVRCSHYP P97096 397 16 -
DILLMKQNNFNAVRCSHYP O09266 382 16 -
DILLMKQHNIKPVRTSHYP O85167 388 17 -
DILLMKQNNFNAVRCSHYP BGAL_KLEPN 382 16 -
DIETMKQHSFNAVRCSHYP BGAL_ENTCL 377 16 -
DILLMKQNNFNAVRCSHYP O09267 387 16 -
DIKLMKEHNFNAVRCSHYP BGAL_LACLA 345 16 -
DIKLMKEHNFNAVRCSHYP O87523 343 16 -
DIKLMKQHNINTVRTSHYP BGAL_THEMA 353 16 -
DIKLMKQHNINTVRTSHYP O85250 353 16 -
DIKVMKQHNINAVRTSHYP BGAL_STRTR 364 16 -
DIKFLKQHNINAVRTSHYP BGAL_CLOAB 365 16 -
DIACMQRNHINAVRTSHYP BGAL_LACSK 371 16 -
DIKTMKRSNINAVRCSHYP BGAL_LACDE 369 16 -
DIQTMLANNINADRTCHYP BGAL_LEULA 372 16 -
DLILMKKFNINAVRNSHYP BGAL_KLULA 373 16 -
DLQLMKQHNINSVRTAHYP BGA2_ECOLI 360 16 -
DLQLMKQHNINSVRTAHYP Q47170 293 16 -
DLALMKRFNVNAIRTSHYP BGAL_ARTSP 345 16 -
DIDTFKENNINAVRTCHYP BGAL_LACAC 374 16 -
DLELMKQGNFNAIRTAHYP BGAL_STAXY 342 16 -
DLQLMKQHNINAIRTAHYP BGAL_ACTPL 339 16 -
DFNLLRWLGANAFRTSHYP BGLR_CANFA 368 16 -
DFNLLRWLGANAFRTSHYP BGLR_HUMAN 369 16 -
DFNLLRWLGANSFRTSHYP BGLR_RAT 365 16 -
DFNLLRWLGANSFRTSHYP BGLR_MOUSE 365 16 -
DHALMDWIGANSYRTSHYP BGLR_ECOLI 314 16 -

Motif 4 width=16
Element Seqn Id St Int Rpt
DKNHPSIIIWSLGNES BGAL_BACME 467 60 -
DRNHPSVIIWSLGNES BGAL_ECOLI 447 53 -
DRNHPSVIIWSLGNES P97096 469 53 -
DRNHPSVIIWSLGNES O09266 454 53 -
DKNHPSIIIWSLGNES O85167 467 60 -
NRNHPCIIIWSLGNES BGAL_KLEPN 454 53 -
DRNHPSIIIWSLGNES BGAL_ENTCL 449 53 -
DRNHPSVIIWSLGNES O09267 459 53 -
DRNHPSIIIWSLGNES BGAL_LACLA 417 53 -
DRNHPSIIIWSLGNES O87523 415 53 -
DKNHPSIIFWSLGNEA BGAL_THEMA 427 55 -
DKNHPSIIFWSLGNEA O85250 427 55 -
DKNHASVIIWSCGNES BGAL_STRTR 444 61 -
DKNHPSVLIWSCGNES BGAL_CLOAB 445 61 -
FKNHVSILFWSLGNES BGAL_LACSK 451 61 -
DKNHASILIWSLGNES BGAL_LACDE 450 62 -
FKNHPSIIFWSLGNES BGAL_LEULA 452 61 -
DVNHPSIIIWSLGNEA BGAL_KLULA 468 76 -
QKNHPSIIIWSLGNES BGA2_ECOLI 435 56 -
QKNHPSIIIWSLGNES Q47170 368 56 -
DKNHASIVMWSLGNES BGAL_ARTSP 420 56 -
FKNHTSILFWSLGNES BGAL_LACAC 454 61 -
LKNYSSIVSWSLGNES BGAL_STAXY 423 62 -
DKNRTSIIIWSLGNEA BGAL_ACTPL 441 83 -
DKNHPSVVMWSVANEP BGLR_CANFA 436 49 -
DKNHPAVVMWSVANEP BGLR_HUMAN 437 49 -
DKNHPAVVMWSVANEP BGLR_RAT 433 49 -
DKNHPAVVMWSVANEP BGLR_MOUSE 433 49 -
DKNHPSVVMWSIANEP BGLR_ECOLI 399 66 -

Motif 5 width=16
Element Seqn Id St Int Rpt
KPYILCEYSHAMGNSC BGAL_BACME 541 58 -
RPLILCEYAHAMGNSL BGAL_ECOLI 531 68 -
RPLILCEYAHAMGNSL P97096 553 68 -
RPLILCEYAHAMGNSL O09266 538 68 -
KPYILCEYSHAMGNSC O85167 541 58 -
RPLILCEYAHAMGNSL BGAL_KLEPN 538 68 -
RPLILCEYAHAMGNSF BGAL_ENTCL 533 68 -
RPLILCEYAHAMGNSL O09267 543 68 -
RPLILCEYAHDMGNSL BGAL_LACLA 502 69 -
RPLILCEYAHDMGNSL O87523 500 69 -
KPFIMCEYAHAMGNSV BGAL_THEMA 501 58 -
KPFIMCEYAHAMGNSV O85250 501 58 -
KPYISCEYMHTMGNSG BGAL_STRTR 540 80 -
KPYISCEYMHSMGNST BGAL_CLOAB 519 58 -
KPFILCEYMHDMGNSL BGAL_LACSK 527 60 -
KPFISVEYAHAMGNSV BGAL_LACDE 525 59 -
KPFLNCEYMHDMGNSL BGAL_LEULA 528 60 -
KPLILCEYGHAMGNGP BGAL_KLULA 545 61 -
KPRIICEYAHAMGNGP BGA2_ECOLI 506 55 -
KPRIICEYAHAMGNGP Q47170 439 55 -
RPFILCEYVHAMGNGP BGAL_ARTSP 507 71 -
KPFMECEYMHDMGNSD BGAL_LACAC 530 60 -
KPFILCEYAHAMGNSP BGAL_STAXY 504 65 -
KPFVLCEYSHAMGNSN BGAL_ACTPL 520 63 -
KPIIQSEYGAETIAGF BGLR_CANFA 533 81 -
KPIIQSEYGAETIAGF BGLR_HUMAN 534 81 -
KPIIQSEYGADAVSGL BGLR_RAT 530 81 -
KPIIQSEYGADAIPGI BGLR_MOUSE 530 81 -
QPIIITEYGVDTLAGL BGLR_ECOLI 497 82 -