| Literature References | 1. PAUDEL, H.K. AND LIAO, T.H.
Purification, characterization, and the complete amino acid sequence of
porcine pancreatic deoxyribonuclease.
J.BIOL.CHEM. 261(34) 16006-16011 (1986).
2. KABSCH, W., MANNHERTZ, H.G., SUCK, D., PAI, E.F. AND HOLMES, K.C.
Atomic structure of the actin - DNase I complex.
NATURE 347 37-44 (1990).
3. SHAK, S., CAPON, D.J., HELLMISS, R., MARSTERS, S.A. AND BAKER, C.L.
Recombinant human DNase I reduces the viscosity of cystic fibrosis sputum.
PROC.NATL.ACAD.SCI.U.S.A. 87(23) 9188-9192 (1990).
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| Documentation | Deoxyribonuclease I (DNase I) is a glycoprotein that causes the cleavage
of the 3'-phosphorous-oxygen bond of the sugar-phosphate DNA backbone,
releasing 5'-phosphoryl nucleotides [1]. The protein is usually secreted
by cells (but can be located in the nucleus), causing cell death by
apoptosis. DNase I forms a 1:1 complex with G-actin, resulting in the
inhibition of DNase activity and loss of the ability of G-actin to
polymerise into fibres [2]. DNase I has been used in the treatment of lung
problems in patients with cystic fibrosis: here it acts by degrading DNA
found in purulent lung secretions, reducing their viscosity and making it
easier for the patient to breathe [3].
DNASEI is an 8-element fingerprint that provides a signature for DNASE I
proteins. The fingerprint was derived from an initial alignment of 3
sequences: the motifs were drawn from conserved regions spanning virtually
the full alignment length, motifs 3 and 4 including the regions encoded by
PROSITE patterns DNASE_I_1 (PS00918) and DNASE_I_2 (PS00919), the latter
containing the catalytically active histidine. Two iterations on OWL22.1
were required to reach convergence, at which point a true set comprising 5
sequences was identified. Two partial matches were also found, NRL_1ATND1
and NRL_1ATND2, both of which are DNase I fragments.
An update on SPTR37_9f identified a true set of 8 sequences, and 6
partial matches.
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