| Literature References | 1. MARTIN, P.R., COOPERIDER, J.W. AND MULKS, M.H.
Sequence of the argf gene encoding ornithine transcarbamoylase from
Neisseria gonorrhoeae.
GENE 94 139-140 (1990).
2. BAUR, H., STALON, V., FALMAGNE, P., LUETHI, E. AND HAAS, D.
Primary and quaternary structure of the catabolic ornithine
carbamoyltransferase from Pseudomonas aeruginosa.
EUR.J.BIOCHEMISTRY 166 111-117 (1987).
3. HUYGEN, R., CRABEEL, M. AND GLANSDORFF, N.
Nucleotide sequence of the ARG3 gene of the yeast Saccharomyces cerevisiae
encoding ornithine carbamoyltransferase.
EUR.J.BIOCHEMISTRY 166 371-377 (1987).
4. UPSHALL, A., GILBERT, T., SAARI, G., O'HARA, P.J., WEGLENSKI, P., BERSE,
B., MILLER, K. AND TIMBERLAKE, W.E.
Molecular analysis of the argB gene of Aspergillus nidulans.
MOL.GEN.GENET. 204 349-354 (1986).
5. MOSQUEDA, G., VAN DEN BROECK, G., SAUCEDO, O., BAILEY, A.M.,
ALVAREZ-MORALES, A. AND HERRERA-ESTRELLA, L.
Isolation and characterization of the gene from Pseudomonas syringae pv.
phaseolicola encoding the phaseolotoxin insensitive ornithine
carbamoyltransferase.
MOL.GEN.GENET. 222 461-466 (1990).
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| Documentation | The ornithine carbamoyltransferases are a family of enzymes that catalyse
the production of citrulline from carbamoyl-phosphate and ornithine [1].
This reaction is part of the arginine biosynthetic pathway, and in some
organisms the reaction is also part of the arginine deaminase pathway [2].
In most prokaryotes, and some lower eukaryotes, the enzyme is found in the
cytoplasm, but in higher eukaryotes, such as mammals, the enzyme is found
in the mitochondrial matrix and functions as part of the urea cycle. In
most organisms analysed to date, the enzyme has been shown to consist of a
trimer of identical or nonidentical subunits [3]. Extensive similarity has
been found within the ornithine carbamoyltransferase family, some of the
conserved areas being important in catalysis [4].
The C-terminal region of these enzymes shows a degree of similarity to the
aspartate carbamoyltransferases, which are also known to bind carbamoyl-
phosphate [5]. This region contains a highly conserved Cys residue (in a
His-Cys-Lys-Pro motif) implicated in ornithine binding [3].
OTCASE is a 5-element fingerprint that provides a signature for ornithine
carbamoyltransferases. The fingerprint was derived from an initial
alignment of 5 sequences: motifs 1 (cf. PROSITE pattern CARBAMOYLTRANSFERASE
(PS00097)) and 3 contain residues thought to be involved in carbamoyl-
phosphate binding. Two iterations on OWL15.0 were required to reach
convergence, at which point a true set comprising 18 sequences was
identified. Several partial matches were also found.
An update on SPTR37_9f identified a true set of 43 sequences, and 7
partial matches.
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| Sequence Titles | O51782 ORNITHINE CARBAMOYLTRANSFERASE, CATABOLIC (ARCB) - BORRELIA BURGDORFERI (LYME DISEASE SPIROCHETE).
O51897 ARCB - BORRELIA AFZELII.
O53089 ORNITHINE TRANSCARBAMOYLASE (EC 2.1.3.3) (ORNITHINE CARBAMOYLTRANSFERASE) (ORNITHINE TRANSCARBAMYLASE) (CITRULLINE PHOSPHORYLASE) (OTCASE) (OTC) - LACTOBACILLUS SAKE.
O58457 317AA LONG HYPOTHETICAL ORNITHINE CARBAMOYLTRANSFERASE - PYROCOCCUS HORIKOSHII.
O67607 ORNITHINE CARBAMOYLTRANSFERASE - AQUIFEX AEOLICUS.
O76458 ORNITHINE CARBAMOYLTRANSFERASE - GIARDIA INTESTINALIS.
O86132 ORNITHINE CARBAMOYLTRANSFERASE (EC 2.1.3.3) - BACILLUS LICHENIFORMIS.
O87319 ORNITHINE CARBAMOYLTRANSFERASE - NOSTOC PCC73102.
OTC1_ECOLI ORNITHINE CARBAMOYLTRANSFERASE CHAIN I (EC 2.1.3.3) (OTCASE-1) - ESCHERICHIA COLI.
OTC2_ECOLI ORNITHINE CARBAMOYLTRANSFERASE CHAIN F (EC 2.1.3.3) (OTCASE-2) - ESCHERICHIA COLI.
OTCA_BACSU ORNITHINE CARBAMOYLTRANSFERASE, ANABOLIC (EC 2.1.3.3) (OTCASE) - BACILLUS SUBTILIS.
OTCA_LACPL ORNITHINE CARBAMOYLTRANSFERASE, ANABOLIC (EC 2.1.3.3) (OTCASE) - LACTOBACILLUS PLANTARUM.
OTCA_MYCBO ORNITHINE CARBAMOYLTRANSFERASE, ANABOLIC (EC 2.1.3.3) (OTCASE) - MYCOBACTERIUM BOVIS.
OTCA_MYCTU ORNITHINE CARBAMOYLTRANSFERASE, ANABOLIC (EC 2.1.3.3) (OTCASE) - MYCOBACTERIUM TUBERCULOSIS.
OTCA_PSEAE ORNITHINE CARBAMOYLTRANSFERASE, ANABOLIC (EC 2.1.3.3) (OTCASE) - PSEUDOMONAS AERUGINOSA.
OTCA_PSESH ORNITHINE CARBAMOYLTRANSFERASE, PHASEOLOTOXIN-SENSITIVE, ANABOLIC (EC 2.1.3.3) (OTCASE) - PSEUDOMONAS SYRINGAE (PV. PHASEOLICOLA).
OTCA_SYNY3 ORNITHINE CARBAMOYLTRANSFERASE, ANABOLIC (EC 2.1.3.3) (OTCASE) - SYNECHOCYSTIS SP. (STRAIN PCC 6803).
OTCA_THEMA ORNITHINE CARBAMOYLTRANSFERASE, ANABOLIC (EC 2.1.3.3) (OTCASE) - THERMOTOGA MARITIMA.
OTCA_VIBS2 ORNITHINE CARBAMOYLTRANSFERASE, ANABOLIC (EC 2.1.3.3) (OTCASE) - VIBRIO SP. (STRAIN 2693).
OTCC_CLOPE ORNITHINE CARBAMOYLTRANSFERASE, CATABOLIC (EC 2.1.3.3) (OTCASE) - CLOSTRIDIUM PERFRINGENS.
OTCC_HAEIN ORNITHINE CARBAMOYLTRANSFERASE, CATABOLIC (EC 2.1.3.3) (OTCASE) - HAEMOPHILUS INFLUENZAE.
OTCC_NEIGO ORNITHINE CARBAMOYLTRANSFERASE, CATABOLIC (EC 2.1.3.3) (OTCASE) - NEISSERIA GONORRHOEAE.
OTCC_PSEAE ORNITHINE CARBAMOYLTRANSFERASE, CATABOLIC (EC 2.1.3.3) (OTCASE) - PSEUDOMONAS AERUGINOSA.
OTCC_PSESH ORNITHINE CARBAMOYLTRANSFERASE, PHASEOLOTOXIN-INSENSITIVE, CATABOLIC (EC 2.1.3.3) (OTCASE) - PSEUDOMONAS SYRINGAE (PV. PHASEOLICOLA), AND PSEUDOMONAS SYRINGAE (PV. ACTINIDIAE).
OTCC_RHIET ORNITHINE CARBAMOYLTRANSFERASE, CATABOLIC (EC 2.1.3.3) (OTCASE) - RHIZOBIUM ETLI.
OTC_ARATH ORNITHINE CARBAMOYLTRANSFERASE PRECURSOR (EC 2.1.3.3) (OTCASE) (ORNITHINE TRANSCARBAMYLASE) - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
OTC_ARCFU ORNITHINE CARBAMOYLTRANSFERASE (EC 2.1.3.3) (OTCASE) - ARCHAEOGLOBUS FULGIDUS.
OTC_ASPNG ORNITHINE CARBAMOYLTRANSFERASE PRECURSOR (EC 2.1.3.3) (OTCASE) (ORNITHINE TRANSCARBAMYLASE) - ASPERGILLUS NIGER.
OTC_ASPTE ORNITHINE CARBAMOYLTRANSFERASE PRECURSOR (EC 2.1.3.3) (OTCASE) (ORNITHINE TRANSCARBAMYLASE) - ASPERGILLUS TERREUS.
OTC_CORHI ORNITHINE CARBAMOYLTRANSFERASE PRECURSOR (EC 2.1.3.3) (OTCASE) (ORNITHINE TRANSCARBAMYLASE) - CORIOLUS HIRSUTUS.
OTC_EMENI ORNITHINE CARBAMOYLTRANSFERASE PRECURSOR (EC 2.1.3.3) (OTCASE) (ORNITHINE TRANSCARBAMYLASE) - EMERICELLA NIDULANS (ASPERGILLUS NIDULANS).
OTC_HALSA ORNITHINE CARBAMOYLTRANSFERASE (EC 2.1.3.3) (OTCASE) - HALOBACTERIUM SALINARIUM.
OTC_HUMAN ORNITHINE CARBAMOYLTRANSFERASE PRECURSOR (EC 2.1.3.3) (OTCASE) (ORNITHINE TRANSCARBAMYLASE) - HOMO SAPIENS (HUMAN).
OTC_METJA ORNITHINE CARBAMOYLTRANSFERASE (EC 2.1.3.3) (OTCASE) - METHANOCOCCUS JANNASCHII.
OTC_METTH ORNITHINE CARBAMOYLTRANSFERASE (EC 2.1.3.3) (OTCASE) - METHANOBACTERIUM THERMOAUTOTROPHICUM.
OTC_MOUSE ORNITHINE CARBAMOYLTRANSFERASE PRECURSOR (EC 2.1.3.3) (OTCASE) (ORNITHINE TRANSCARBAMYLASE) - MUS MUSCULUS (MOUSE).
OTC_PACTA ORNITHINE CARBAMOYLTRANSFERASE PRECURSOR (EC 2.1.3.3) (OTCASE) (ORNITHINE TRANSCARBAMYLASE) - PACHYSOLEN TANNOPHILUS (YEAST).
OTC_PEA ORNITHINE CARBAMOYLTRANSFERASE PRECURSOR (EC 2.1.3.3) (OTCASE) (ORNITHINE TRANSCARBAMYLASE) - PISUM SATIVUM (GARDEN PEA).
OTC_PYRFU ORNITHINE CARBAMOYLTRANSFERASE (EC 2.1.3.3) (OTCASE) - PYROCOCCUS FURIOSUS.
OTC_RANCA ORNITHINE CARBAMOYLTRANSFERASE PRECURSOR (EC 2.1.3.3) (OTCASE) (ORNITHINE TRANSCARBAMYLASE) - RANA CATESBEIANA (BULL FROG).
OTC_RAT ORNITHINE CARBAMOYLTRANSFERASE PRECURSOR (EC 2.1.3.3) (OTCASE) (ORNITHINE TRANSCARBAMYLASE) - RATTUS NORVEGICUS (RAT).
OTC_SCHPO ORNITHINE CARBAMOYLTRANSFERASE PRECURSOR (EC 2.1.3.3) (OTCASE) (ORNITHINE TRANSCARBAMYLASE) - SCHIZOSACCHAROMYCES POMBE (FISSION YEAST).
OTC_YEAST ORNITHINE CARBAMOYLTRANSFERASE (EC 2.1.3.3) (OTCASE) (ORNITHINE TRANSCARBAMYLASE) - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
OTCC_MYCPN ORNITHINE CARBAMOYLTRANSFERASE, CATABOLIC (EC 2.1.3.3) (OTCASE) - MYCOPLASMA PNEUMONIAE.
O15804 ASPARTATE TRANSCARBAMOYLASE (EC 2.1.3.2) - PLASMODIUM FALCIPARUM.
PYRB_ARCFU ASPARTATE CARBAMOYLTRANSFERASE (EC 2.1.3.2) (ASPARTATE TRANSCARBAMYLASE) (ATCASE) - ARCHAEOGLOBUS FULGIDUS.
PYRB_METJA ASPARTATE CARBAMOYLTRANSFERASE (EC 2.1.3.2) (ASPARTATE TRANSCARBAMYLASE) (ATCASE) - METHANOCOCCUS JANNASCHII.
PYRB_MYCTU ASPARTATE CARBAMOYLTRANSFERASE (EC 2.1.3.2) (ASPARTATE TRANSCARBAMYLASE) (ATCASE) - MYCOBACTERIUM TUBERCULOSIS.
PYRB_SERMA ASPARTATE CARBAMOYLTRANSFERASE CATALYTIC CHAIN (EC 2.1.3.2) (ASPARTATE TRANSCARBAMYLASE) (ATCASE) - SERRATIA MARCESCENS.
PYRB_SYNY3 ASPARTATE CARBAMOYLTRANSFERASE (EC 2.1.3.2) (ASPARTATE TRANSCARBAMYLASE) (ATCASE) - SYNECHOCYSTIS SP. (STRAIN PCC 6803).
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