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PR00089

Identifier
LUCIFERASE  [View Relations]  [View Alignment]  
Accession
PR00089
No. of Motifs
5
Creation Date
03-AUG-1992  (UPDATE 07-JUN-1999)
Title
Bacterial luciferase signature
Database References

PROSITE; PS00494 BACTERIAL_LUCIFERASE
BLOCKS; BL00494
PFAM; PF00296 bac_luciferase
INTERPRO; IPR002103
Literature References
1. MEIGHEN, E.A.
Molecular biology of bacterial luminescence.
MICROBIOL.REV. 55 123-142 (1991).
 
2. O'KANE, D.J.O. AND PRASHER, D.C.
Evolutionary origins of bacterial bioluminescence.
MOL.MICROBIOL. 6 443-449 (1992).

Documentation
The enzyme responsible for bioluminescence in motile Gram-negative luminous
bacteria is bacterial luciferase [1,2], which catalyses the oxidation of
reduced riboflavin phosphate and a long-chain fatty aldehyde, with the
emission of blue-green light (490nm). Luciferase is a heterodimeric enzyme
comprising alpha and beta subunits (genes luxA and luxB), which appear to 
have arisen through gene duplication.
 
Some species of Photobacterium contain a bioluminescence operon that 
encodes a non-fluorescent flavoprotein (NFP) (gene luxF). The function of 
NFP is not known, but it is clearly related to the luxA and luxB subunits.
 
LUCIFERASE is a 5-element fingerprint that provides a signature for the
bacterial luciferases. The fingerprint was derived from an initial
alignment of 7 sequences: motifs 2 and 3 span the region encoded by PROSITE
pattern BACTERIAL_LUCIFERASE (PS00494). Four iterations on OWL17.1 were
required to reach convergence, at which point a true set comprising 18
sequences was identified. A single partial match was also found, the non-
fluorescent flavoprotein LUXF_PHOLE, which fails to make significant
matches with motifs 1 and 3. 
 
An update on SPTR37_9f identified a true set of 22 sequences.
Summary Information
22 codes involving  5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
52222222222
400000
300000
200000
12345
True Positives
LUXA_KRYAS    LUXA_PHOPO    LUXA_VIBFI    LUXA_VIBHA    
LUXB_PHOPO LUXB_VIBFI LUXB_VIBHA LUXF_PHOPO
LXA1_PHOLE LXA1_PHOLU LXA2_PHOLE LXA2_PHOLU
LXB1_PHOLE LXB1_PHOLU LXB2_PHOLE LXB2_PHOLU
Q52098 Q52099 Q56698 Q56699
Q56821 Q56822
Sequence Titles
LUXA_KRYAS  ALKANAL MONOOXYGENASE ALPHA CHAIN (EC 1.14.14.3) (BACTERIAL LUCIFERASE ALPHA CHAIN) - KRYPTOPHANARON ALFREDI SYMBIONT. 
LUXA_PHOPO ALKANAL MONOOXYGENASE ALPHA CHAIN (EC 1.14.14.3) (BACTERIAL LUCIFERASE ALPHA CHAIN) - PHOTOBACTERIUM PHOSPHOREUM.
LUXA_VIBFI ALKANAL MONOOXYGENASE ALPHA CHAIN (EC 1.14.14.3) (BACTERIAL LUCIFERASE ALPHA CHAIN) - VIBRIO FISCHERI.
LUXA_VIBHA ALKANAL MONOOXYGENASE ALPHA CHAIN (EC 1.14.14.3) (BACTERIAL LUCIFERASE ALPHA CHAIN) - VIBRIO HARVEYI.
LUXB_PHOPO ALKANAL MONOOXYGENASE BETA CHAIN (EC 1.14.14.3) (BACTERIAL LUCIFERASE BETA CHAIN) - PHOTOBACTERIUM PHOSPHOREUM.
LUXB_VIBFI ALKANAL MONOOXYGENASE BETA CHAIN (EC 1.14.14.3) (BACTERIAL LUCIFERASE BETA CHAIN) - VIBRIO FISCHERI.
LUXB_VIBHA ALKANAL MONOOXYGENASE BETA CHAIN (EC 1.14.14.3) (BACTERIAL LUCIFERASE BETA CHAIN) - VIBRIO HARVEYI.
LUXF_PHOPO NON-FLUORESCENT FLAVOPROTEIN (NFP) (FP390) - PHOTOBACTERIUM PHOSPHOREUM.
LXA1_PHOLE ALKANAL MONOOXYGENASE ALPHA CHAIN (EC 1.14.14.3) (BACTERIAL LUCIFERASE ALPHA CHAIN) - PHOTOBACTERIUM LEIOGNATHI.
LXA1_PHOLU ALKANAL MONOOXYGENASE ALPHA CHAIN (EC 1.14.14.3) (BACTERIAL LUCIFERASE ALPHA CHAIN) - PHOTORHABDUS LUMINESCENS (XENORHABDUS LUMINESCENS).
LXA2_PHOLE ALKANAL MONOOXYGENASE ALPHA CHAIN (EC 1.14.14.3) (BACTERIAL LUCIFERASE ALPHA CHAIN) - PHOTOBACTERIUM LEIOGNATHI.
LXA2_PHOLU ALKANAL MONOOXYGENASE ALPHA CHAIN (EC 1.14.14.3) (BACTERIAL LUCIFERASE ALPHA CHAIN) - PHOTORHABDUS LUMINESCENS (XENORHABDUS LUMINESCENS).
LXB1_PHOLE ALKANAL MONOOXYGENASE BETA CHAIN (EC 1.14.14.3) (BACTERIAL LUCIFERASE BETA CHAIN) - PHOTOBACTERIUM LEIOGNATHI.
LXB1_PHOLU ALKANAL MONOOXYGENASE BETA CHAIN (EC 1.14.14.3) (BACTERIAL LUCIFERASE BETA CHAIN) - PHOTORHABDUS LUMINESCENS (XENORHABDUS LUMINESCENS).
LXB2_PHOLE ALKANAL MONOOXYGENASE BETA CHAIN (EC 1.14.14.3) (BACTERIAL LUCIFERASE BETA CHAIN) - PHOTOBACTERIUM LEIOGNATHI.
LXB2_PHOLU ALKANAL MONOOXYGENASE BETA CHAIN (EC 1.14.14.3) (BACTERIAL LUCIFERASE BETA CHAIN) - PHOTORHABDUS LUMINESCENS (XENORHABDUS LUMINESCENS).
Q52098 ALPHA LUCIFERASE SUBUNIT - PHOTOBACTERIUM PHOSPHOREUM.
Q52099 BETA LUCIFERASE SUBUNIT - PHOTOBACTERIUM PHOSPHOREUM.
Q56698 LUCIFERASE ALPHA SUBUNIT - VIBRIO HARVEYI.
Q56699 LUCIFERASE BETA SUBUNIT - VIBRIO HARVEYI.
Q56821 LUCIFERASE - XENORHABDUS LUMINESCENS.
Q56822 LUCIFERASE - XENORHABDUS LUMINESCENS.
Scan History
OWL17_1    4  980  NSINGLE    
OWL18_0 1 50 NSINGLE
OWL19_1 1 50 NSINGLE
OWL26_0 1 100 NSINGLE
SPTR37_9f 2 23 NSINGLE
Initial Motifs
Motif 1  width=12
Element Seqn Id St Int Rpt
GAPIYVVAESAS LXA2_XENLU 166 166 -
GAPVYVVAESAS LUXA_KRYAL 166 166 -
GPRKYVTATSHH LXB1_XENLU 166 166 -
GPRKYVTATSCH LXB2_XENLU 166 166 -
GAPVYVVAESAS LXA1_XENLU 166 166 -
GEKLYVLATSEN LUXF_PHOPO 68 68 -
NMKQYILASSVS LUXB_PHOPO 163 163 -

Motif 2 width=16
Element Seqn Id St Int Rpt
EWAAQHGLPMILSWII LXA2_XENLU 180 2 -
EWAAQFGLPMILSWII LXA1_XENLU 180 2 -
EWAAERGLPIILSWII LUXA_KRYAL 180 2 -
EWAAKKGIPLIFKWDD LXB1_XENLU 180 2 -
EWAAKKGIPLIFKWDD LXB2_XENLU 180 2 -
DIAAKYALPLVFKWDD LUXF_PHOPO 82 2 -
EWAAKKALPLTYRWSD LUXB_PHOPO 176 1 -

Motif 3 width=16
Element Seqn Id St Int Rpt
INEERLKLLSFYNASA LUXF_PHOPO 98 0 -
NNNEKKSQLDLYNEIA LUXA_KRYAL 196 0 -
NTNEKKAQLELYNEVA LXA1_XENLU 196 0 -
NTNDKKAQIELYNEVA LXA2_XENLU 196 0 -
SNDVRYEYAERYKAVA LXB1_XENLU 196 0 -
SNEVKHEYAKRYQAIA LXB2_XENLU 196 0 -
TLEDKEILYKRYLEVA LUXB_PHOPO 192 0 -

Motif 4 width=18
Element Seqn Id St Int Rpt
EYGHDIHNIDHCLSYITS LXA2_XENLU 213 1 -
EHGHDVSNIDHCMSYITS LUXA_KRYAL 213 1 -
EYGHDIHNIDHCLSYITS LXA1_XENLU 213 1 -
KYDVDLSEIDHQLMILVN LXB1_XENLU 213 1 -
EYGVDLAEIDHQLMILVN LXB2_XENLU 213 1 -
KHNIDVSNVEHQFPLLVN LUXB_PHOPO 208 0 -
KYNKNIDLVRHQLMLHVN LUXF_PHOPO 115 1 -

Motif 5 width=19
Element Seqn Id St Int Rpt
VDHDSMKAKEICRNFLGHW LXA2_XENLU 231 0 -
VDHDSIKAKEICRKFLGHW LXA1_XENLU 231 0 -
VDHNSNKAKDICRDFLAHW LUXA_KRYAL 231 0 -
LNHDRDVAHQEATAYVSYI LUXB_PHOPO 226 0 -
VNEAETVAKEELKLYIENY LUXF_PHOPO 133 0 -
YSEDSEKAKEETRAFISDY LXB2_XENLU 231 0 -
YNEDSNKAKQETRAFISDY LXB1_XENLU 231 0 -
Final Motifs
Motif 1  width=12
Element Seqn Id St Int Rpt
GAPIYVVAESAS Q56821 167 167 -
GAPVYVVAESAS LXA1_PHOLU 167 167 -
GAPIYVVAESAS LXA2_PHOLU 167 167 -
GAPVYVVAESAS LUXA_VIBHA 167 167 -
GAPVYVVAESAS Q56698 167 167 -
GAPVYVVAESAS LUXA_KRYAS 167 167 -
KIPTCMTAESAA LXA1_PHOLE 166 166 -
NVPTCMTAESAS LUXA_VIBFI 166 166 -
KIPTCMTAESAV Q52098 166 166 -
GPRKYVTATSCH LXB2_PHOLU 167 167 -
GPRKYVTATSCH Q56822 167 167 -
KQPTCMTAESSE LXA2_PHOLE 166 166 -
GPRKYVTATSHH LXB1_PHOLU 167 167 -
GPAQFVNATSKE Q56699 167 167 -
GPAQFVNATSKE LUXB_VIBHA 167 167 -
GPKQYVSATSKE LUXB_VIBFI 169 169 -
NLKQYILATSMG LXB1_PHOLE 169 169 -
KIPTCMTAESAT LUXA_PHOPO 161 161 -
EVKQYILASSMG LXB2_PHOLE 167 167 -
NMKQYILASSVS Q52099 169 169 -
NMKQYILASSVS LUXB_PHOPO 163 163 -
GEKLYVLATSEN LUXF_PHOPO 68 68 -

Motif 2 width=16
Element Seqn Id St Int Rpt
EWAAQHGLPMILSWII Q56821 181 2 -
EWAAQFGLPMILSWII LXA1_PHOLU 181 2 -
EWAAQHGLPMILSWII LXA2_PHOLU 181 2 -
EWAAERGLPMILSWII LUXA_VIBHA 181 2 -
EWAAERGLPMILSWII Q56698 181 2 -
EWAAERGLPIILSWII LUXA_KRYAS 181 2 -
TWLAERGLPMVLSWII LXA1_PHOLE 180 2 -
EWLAIQGLPMVLSWII LUXA_VIBFI 180 2 -
TWLAERGLPMVLSWII Q52098 180 2 -
EWAAKKGIPLIFKWDD LXB2_PHOLU 181 2 -
EWAAKKGIPLIFKWDD Q56822 181 2 -
TYLAERGLPMVLSWII LXA2_PHOLE 180 2 -
EWAAKKGIPLIFKWDD LXB1_PHOLU 181 2 -
EWAAKLGLPLVFKWDD Q56699 181 2 -
EWAAKLGLPLVFRWDD LUXB_VIBHA 181 2 -
MWAAKKALPLTFKWED LUXB_VIBFI 183 2 -
EWAAKKGLPLTYRWSD LXB1_PHOLE 183 2 -
TWLAERGLPMVLSWII LUXA_PHOPO 174 1 -
EWAARKGLPLTYRWSD LXB2_PHOLE 181 2 -
EWAAKKALPLTYRWSD Q52099 183 2 -
EWAAKKALPLTYRWSD LUXB_PHOPO 176 1 -
DIAAKYALPLVFKWDD LUXF_PHOPO 82 2 -

Motif 3 width=16
Element Seqn Id St Int Rpt
NTNEKKAQIELYNEVA Q56821 197 0 -
NTNEKKAQLELYNEVA LXA1_PHOLU 197 0 -
NTNDKKAQIELYNEVA LXA2_PHOLU 197 0 -
NTHEKKAQLDLYNEVA LUXA_VIBHA 197 0 -
NTHEKKAHVDLYNEVA Q56698 197 0 -
NNNEKKSQLDLYNEIA LUXA_KRYAS 197 0 -
TTSEKKAQMELYNEIA LXA1_PHOLE 196 0 -
GTNEKKAQMELYNEIA LUXA_VIBFI 196 0 -
TTSEKKAQMELYNAVA Q52098 196 0 -
SNEVKHEYAKRYQAIA LXB2_PHOLU 197 0 -
SNEVKHEYAKRYQAIA Q56822 197 0 -
PVSEKVSQMELYNEVA LXA2_PHOLE 196 0 -
SNDVRYEYAERYKAVA LXB1_PHOLU 197 0 -
SNAQRKEYAGLYHEVA Q56699 197 0 -
SNAQRKEYAGLYHEVA LUXB_VIBHA 197 0 -
NLETKERYAILYNKTA LUXB_VIBFI 199 0 -
TLAEKENYYQRYLTVA LXB1_PHOLE 199 0 -
TTSEKKAQMELYNAVR LUXA_PHOPO 190 0 -
SLAEKEKYYQRYLAVA LXB2_PHOLE 197 0 -
TLEDKEILYKRYLEVA Q52099 199 0 -
TLEDKEILYKRYLEVA LUXB_PHOPO 192 0 -
INEERLKLLSFYNASA LUXF_PHOPO 98 0 -

Motif 4 width=18
Element Seqn Id St Int Rpt
EYGHDIHNIDHCLSYITS Q56821 214 1 -
EYGHDIHNIDHCLSYITS LXA1_PHOLU 214 1 -
EYGHDIHNIDHCLSYITS LXA2_PHOLU 214 1 -
EHGYDVTKIDHCLSYITS LUXA_VIBHA 214 1 -
EHGYDVTKIDHCLSYITS Q56698 214 1 -
EHGHDVSNIDHCMSYITS LUXA_KRYAS 214 1 -
EHGHDIHNIDHSMTFICS LXA1_PHOLE 213 1 -
EYGHDISKIDHCMTYICS LUXA_VIBFI 213 1 -
YSEEYIKNVDHSMTLICS Q52098 216 4 -
EYGVDLAEIDHQLMILVN LXB2_PHOLU 214 1 -
EYGVDLAEIDHQLMILVN Q56822 214 1 -
EHGHDINNIEHILTFICS LXA2_PHOLE 213 1 -
KYDVDLSEIDHQLMILVN LXB1_PHOLU 214 1 -
AHGVDVSQVRHKLTLLVN Q56699 214 1 -
AHGVDVSQVRHKLTLLVN LUXB_VIBHA 214 1 -
QYGIDISDVDHQLTVIAN LUXB_VIBFI 216 1 -
ENNVDITHVDHQFPLLVN LXB1_PHOLE 216 1 -
YSEEYIKNVDHSMTLICS LUXA_PHOPO 209 3 -
ENNIDVSNIDHQFPLLVN LXB2_PHOLE 214 1 -
KHNIDVSNVEHQFPLLVN Q52099 216 1 -
KHNIDVSNVEHQFPLLVN LUXB_PHOPO 208 0 -
KYNKNIDLVRHQLMLHVN LUXF_PHOPO 115 1 -

Motif 5 width=19
Element Seqn Id St Int Rpt
VDHDSMKAKEICRNFLGHW Q56821 232 0 -
VDHDSIKAKEICRKFLGHW LXA1_PHOLU 232 0 -
VDHDSMKAKEICRNFLGHW LXA2_PHOLU 232 0 -
VDHDSNRAKDICRNFLGHW LUXA_VIBHA 232 0 -
VDHDSNKAKDICRNFLGHW Q56698 232 0 -
VDHNSNKAKDICRDFLAHW LUXA_KRYAS 232 0 -
VNEDPEKAESVCRDFLSNW LXA1_PHOLE 231 0 -
VDDDAQKAQDVCREFLKNW LUXA_VIBFI 231 0 -
VDEDGKKAEDVCREFLGNW Q52098 234 0 -
YSEDSEKAKEETRAFISDY LXB2_PHOLU 232 0 -
YSEDSEKAKEETRAFISDY Q56822 232 0 -
VNEDGEKADSVCRNFLENW LXA2_PHOLE 231 0 -
YNEDSNKAKQETRAFISDY LXB1_PHOLU 232 0 -
QNVDGEAARAEARVYLEEF Q56699 232 0 -
QNVDGEAARAEARVYLEEF LUXB_VIBHA 232 0 -
LNADRSTAQEEVREYLKDY LUXB_VIBFI 234 0 -
INPDRDIAKQEMRDYIRGY LXB1_PHOLE 234 0 -
VDEDGKKAEDVREFLGNWY LUXA_PHOPO 227 0 -
INENRRIARDEVREYIQSY LXB2_PHOLE 232 0 -
LNHDRDVAHQEATAYLVSY Q52099 234 0 -
LNHDRDVAHQEATAYVSYI LUXB_PHOPO 226 0 -
VNEAETVAKEELKLYIENY LUXF_PHOPO 133 0 -