| Literature References | 1. MADSEN, O., SANDAL, L., SANDAL, N.N. AND MARCKER, K.A.
A soybean coproporphyrinogen oxidase gene is highly expressed in
root nodules.
PLANT MOL.BIOL. 23 35-43 (1993).
2. KOHNO, H., FURUKAWA, T., YOSHINAGA, T., TOKUNAGA, R. AND TAKETANI, S.
Coproporphyrin oxidase. Purification, molecular cloning, and induction of
mRNA during erythroid differentiation.
J.BIOL.CHEM. 268 21359-21363 (1993).
3. CAMADRO, J.M., CHAMBON, H., JOLLES, J. AND LABBE, P.
Purification and properties of coproporphyrinogen oxidase from the yeast
Saccharomyces cerevisiae.
EUR.J.BIOCHEMISTRY 156 579-587 (1986).
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| Documentation | Coprogen oxidase (i.e. coproporphyrin III oxidase or coproporphyrinogenase)
catalyses the oxidative decarboxylation of coproporphyrinogen III to proto-
porhyrinogen IX in the haem and chlorophyll biosynthetic pathways [1,2].
The protein is a homodimer containing 2 iron atoms per molecule of native
protein [3]. The iron atoms can be removed neither by exhaustive dialysis
nor by gel filtration, indicating that they are internally bound [3]: their
involvement in the catalytic activity cannot be demonstrated, the enzyme
only being active in the presence of molecular oxygen (acting as the
electron acceptor) [3]. Thiol-directed agents partially inhibit catalytic
activity, indicating that an SH group is required for activity [3].
COPRGNOXDASE is a 7-element fingerprint that provides a signature for
coprogen oxidases. The fingerprint was derived from an initial alignment
of 6 sequences: the motifs were drawn from conserved regions spanning
virtually the full alignment length, motif 5 including the region encoded
by PROSITE pattern COPROGEN_OXIDASE (PS01021), a highly-conserved charged
area containing the putative catalytically-important Cys. Two iterations on
OWL25.1 were required to reach convergence, at which point a true set
comprising 7 sequences was identified.
An update on SPTR37_9f identified a true set of 11 sequences, and 1
partial match.
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| Sequence Titles | HEM6_ECOLI COPROPORPHYRINOGEN III OXIDASE, AEROBIC (EC 1.3.3.3) (COPROPORPHYRINOGENASE) (COPROGEN OXIDASE) - ESCHERICHIA COLI.
HEM6_HORVU COPROPORPHYRINOGEN III OXIDASE PRECURSOR (EC 1.3.3.3) (COPROPORPHYRINOGENASE) (COPROGEN OXIDASE) - HORDEUM VULGARE (BARLEY).
HEM6_HUMAN COPROPORPHYRINOGEN III OXIDASE PRECURSOR (EC 1.3.3.3) (COPROPORPHYRINOGENASE) (COPROGEN OXIDASE) (COX) - HOMO SAPIENS (HUMAN).
HEM6_MOUSE COPROPORPHYRINOGEN III OXIDASE PRECURSOR (EC 1.3.3.3) (COPROPORPHYRINOGENASE) (COPROGEN OXIDASE) (COX) - MUS MUSCULUS (MOUSE).
HEM6_PSEAE COPROPORPHYRINOGEN III OXIDASE, AEROBIC (EC 1.3.3.3) (COPROPORPHYRINOGENASE) (COPROGEN OXIDASE) - PSEUDOMONAS AERUGINOSA.
HEM6_SALTY COPROPORPHYRINOGEN III OXIDASE, AEROBIC (EC 1.3.3.3) (COPROPORPHYRINOGENASE) (COPROGEN OXIDASE) - SALMONELLA TYPHIMURIUM.
HEM6_SOYBN COPROPORPHYRINOGEN III OXIDASE PRECURSOR (EC 1.3.3.3) (COPROPORPHYRINOGENASE) (COPROGEN OXIDASE) - GLYCINE MAX (SOYBEAN).
HEM6_SYNY3 COPROPORPHYRINOGEN III OXIDASE, AEROBIC (EC 1.3.3.3) (COPROPORPHYRINOGENASE) (COPROGEN OXIDASE) - SYNECHOCYSTIS SP. (STRAIN PCC 6803).
HEM6_TOBAC COPROPORPHYRINOGEN III OXIDASE PRECURSOR (EC 1.3.3.3) (COPROPORPHYRINOGENASE) (COPROGEN OXIDASE) - NICOTIANA TABACUM (COMMON TOBACCO).
HEM6_YEAST COPROPORPHYRINOGEN III OXIDASE (EC 1.3.3.3) (COPROPORPHYRINOGENASE) (COPROGEN OXIDASE) (COX) - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
Q14060 COPROPORPHYRINOGEN OXIDASE - HOMO SAPIENS (HUMAN).
O48754 F21B7.24 - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
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