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PR00015

Identifier
GPOSANCHOR  [View Relations]  [View Alignment]  
Accession
PR00015
No. of Motifs
2
Creation Date
15-SEP-1993  (UPDATE 19-JUN-1999)
Title
Gram-positive coccus surface protein anchor signature
Database References

PROSITE; PS00343 GRAM_POS_ANCHORING
BLOCKS; BL00343
PFAM; PF00746 Gram_pos_anchor
INTERPRO; IPR001899
Literature References
1. FISCHETTI, V.
Streptococcal M protein.
SCI.AM. 134-141 (1993).
 
2. SCHNEEWIND, O., JONES, K.F. AND FISCHETTI, V.A.
Sequence and structural characteristics of the trypsin-resistant T6 surface
protein of group-A streptococci.
J.BACTERIOL. 172(6) 3310-3317 (1990).
 
3. FISCHETTI, V.A., PANCHOLI, V. AND SCHNEEWIND, O.
Conservation of a hexapeptide sequence in the anchor region of surface-
proteins from Gram-positive cocci.
MOL.MICROBIOL. 4(9) 1603-1605 (1990).

Documentation
Viruses, parasites and bacteria are covered in protein and sugar molecules
that help them gain entry into a host by counteracting the host's defenses
[1]. One such molecule is the M protein produced by certain streptococcal
bacteria. M proteins embody a motif that is now known to be shared by many 
bacterial surface proteins. The motif includes a conserved hexapeptide,
which precedes a hydrophobic C-terminal membrane anchor, which itself
precedes a cluster of basic residues [2,3]. The hexapeptide is suggested
to be essential for the correct anchoring to the bacterial membrane - the
hydrophobic domain alone, with its C-terminal charged residues, is
insufficient for attachment. The proteins that contain the conserved
hexapeptide and hydrophobic anchor are diverse: they include M proteins,
IgA and IgG binding proteins, fibronectin-binding proteins, wall-associated
proteins, trypsin-resistant surface T protein, protein H precursor, etc..
 
GPOSANCHOR is a 2-element fingerprint that provides a signature for the
membrane anchor region of such cell-surface proteins. The fingerprint was
derived from an initial alignment of 6 sequences: motif 1 includes the well-
conserved hexapeptide encoded by PROSITE pattern GRAM_POS_ANCHORING
(PS00343), and motif 2 spans the hydrophobic C-terminal anchor and part
of the basic residue cluster. Three iterations on OWL21.1 were required to
reach convergence, at which point a true set comprising 40 sequences was
identified. The diagnostic performance of this fingerprint is not high,
largely because it contains only 2 motifs, coupled with the fact that the
degree of sequence similarity within the family is poor. As a result, 
it has been necessary to use large hitlists to capture as many true-
positives as possible. Note also that the fingerprint encodes both the
conserved hexapeptide and the hydrophobic transmembrane motif, and hence
sequences only bearing the hexapeptide are not identified. 
 
An update on SPTR37_9f identified a true set of 58 sequences.
Summary Information
58 codes involving  2 elements
Composite Feature Index
25858
12
True Positives
ARP4_STRPY    BCA_STRAG     M21_STRPY     M22_STRPY     
M24_STRPY M49_STRPY M5_STRPY M6_STRPY
MRP4_STRPY MX_STRPY O33631 O33898
O33899 O68165 P72362 P95808
P95810 P95813 Q00720 Q05464
Q10372 Q53474 Q53475 Q53476
Q53974 Q53975 Q54071 Q54511
Q54555 Q54703 Q54718 Q54719
Q54744 Q54745 Q54746 Q54829
Q54835 Q54837 Q54839 Q54840
Q54841 Q54842 Q54843 Q54849
Q54850 Q54851 Q54859 Q54860
Q54876 Q54901 Q55098 Q55105
Q55246 Q55312 Q56212 SPG1_STRSP
SPG2_STRSP SPH_STRPY
Sequence Titles
ARP4_STRPY  IGA RECEPTOR PRECURSOR - STREPTOCOCCUS PYOGENES. 
BCA_STRAG C PROTEIN ALPHA-ANTIGEN PRECURSOR - STREPTOCOCCUS AGALACTIAE.
M21_STRPY M PROTEIN, SEROTYPE 2.1 PRECURSOR - STREPTOCOCCUS PYOGENES.
M22_STRPY M PROTEIN, SEROTYPE 2.2 PRECURSOR - STREPTOCOCCUS PYOGENES.
M24_STRPY M PROTEIN, SEROTYPE 24 PRECURSOR - STREPTOCOCCUS PYOGENES.
M49_STRPY M PROTEIN, SEROTYPE 49 PRECURSOR - STREPTOCOCCUS PYOGENES.
M5_STRPY M PROTEIN, SEROTYPE 5 PRECURSOR - STREPTOCOCCUS PYOGENES.
M6_STRPY M PROTEIN, SEROTYPE 6 PRECURSOR - STREPTOCOCCUS PYOGENES.
MRP4_STRPY FIBRINOGEN- AND IG-BINDING PROTEIN PRECURSOR (MRP PROTEIN) - STREPTOCOCCUS PYOGENES.
MX_STRPY VIRULENCE FACTOR-RELATED M PROTEIN PRECURSOR - STREPTOCOCCUS PYOGENES.
O33631 M-LIKE PROTEIN - STREPTOCOCCUS EQUISIMILIS.
O33898 M-PROTEIN - STREPTOCOCCUS EQUI.
O33899 M-LIKE PROTEIN - STREPTOCOCCUS EQUI.
O68165 FIBRINOGEN-BINDING PROTEIN - STREPTOCOCCUS EQUI.
P72362 SURFACE PROTEIN RIB - STREPTOCOCCUS AGALACTIAE.
P95808 FC-GAMMA RECEPTOR PRECURSOR - STREPTOCOCCUS PYOGENES.
P95810 (STRAIN M8-4025) - STREPTOCOCCUS PYOGENES.
P95813 FC-GAMMA RECEPTOR PRECURSOR - STREPTOCOCCUS PYOGENES.
Q00720 M PROTEIN PRECURSOR - GROUP G STREPTOCOCCUS.
Q05464 M PROTEIN, SEROTYPE 1.1 PRECURSOR - STREPTOCOCCUS PYOGENES.
Q10372 M PROTEIN, SEROTYPE 1.2 PRECURSOR - STREPTOCOCCUS PYOGENES.
Q53474 MRP4 - STREPTOCOCCUS PYOGENES.
Q53475 EMML15 - STREPTOCOCCUS PYOGENES.
Q53476 ENN15 - STREPTOCOCCUS PYOGENES.
Q53974 (MAG) - STREPTOCOCCUS DYSGALACTIAE.
Q53975 IMMUNOGLOBIN G BINDING PROTEIN MIG PRECURSOR (IGG BINDING PROTEIN MIG) - STREPTOCOCCUS DYSGALACTIAE.
Q54071 M PROTEIN PRECURSOR, MSZW60 - STREPTOCOCCUS EQUI.
Q54511 ENN5.8193 PROTEIN - STREPTOCOCCUS PYOGENES.
Q54555 M25 PROTEIN PRECURSOR (M TYPE 25) (EMML) - STREPTOCOCCUS PYOGENES.
Q54703 EMM18.1 - STREPTOCOCCUS PYOGENES.
Q54718 M PROTEIN - STREPTOCOCCUS PYOGENES.
Q54719 M3 PROTEIN - STREPTOCOCCUS PYOGENES.
Q54744 MRP50 - STREPTOCOCCUS PYOGENES.
Q54745 EMM50 - STREPTOCOCCUS PYOGENES.
Q54746 ENN50 - STREPTOCOCCUS PYOGENES.
Q54829 M PROTEIN PRECURSOR - STREPTOCOCCUS PYOGENES.
Q54835 M3 PROTEIN PRECURSOR - STREPTOCOCCUS PYOGENES.
Q54837 M PROTEIN TYPE 41 - STREPTOCOCCUS PYOGENES.
Q54839 M PROTEIN TYPE 52 - STREPTOCOCCUS PYOGENES.
Q54840 M PROTEIN - STREPTOCOCCUS PYOGENES.
Q54841 M-TYPE 9 PROTEIN - STREPTOCOCCUS PYOGENES.
Q54842 GENES FOR FCR PROTEIN AND M PROTEIN PRECURSOR - STREPTOCOCCUS PYOGENES.
Q54843 GENES FOR FCR PROTEIN AND M PROTEIN PRECURSOR - STREPTOCOCCUS PYOGENES.
Q54849 ENN18 GENE PRECURSOR - STREPTOCOCCUS PYOGENES.
Q54850 IMMUNOGLOBULIN-FC-BINDING PROTEIN - STREPTOCOCCUS PYOGENES.
Q54851 ENN PROTEIN - STREPTOCOCCUS PYOGENES.
Q54859 FCRA IMMUNOGLOBULIN-BINDING PROTEIN PRECURSOR - STREPTOCOCCUS PYOGENES.
Q54860 IMMUNOGLOBULIN-FC-BINDING PROTEIN - STREPTOCOCCUS PYOGENES.
Q54876 IGA RECEPTOR PROTEIN PRECURSOR - STREPTOCOCCUS PYOGENES.
Q54901 PRECURSOR TO PROTEIN SIR22 - STREPTOCOCCUS PYOGENES.
Q55098 M PROTEIN - STREPTOCOCCUS SP.
Q55105 MULTIPLE LIGAND-BINDING PROTEIN 1 PRECURSOR - STREPTOCOCCUS SP.
Q55246 M PROTEIN - STREPTOCOCCUS SP.
Q55312 PROTEIN V PRECURSOR - STREPTOCOCCUS SP.
Q56212 CELL SURFACE PROTEIN PRECURSOR - STREPTOCOCCUS ZOOEPIDEMICUS.
SPG1_STRSP IMMUNOGLOBULIN G BINDING PROTEIN G PRECURSOR (IGG BINDING PROTEIN G) - STREPTOCOCCUS SP. (LANCEFIELD GROUP G).
SPG2_STRSP IMMUNOGLOBULIN G BINDING PROTEIN G PRECURSOR (IGG BINDING PROTEIN G) - STREPTOCOCCUS SP. (STRAIN G148).
SPH_STRPY IMMUNOGLOBULIN G BINDING PROTEIN H PRECURSOR (IGG BINDING PROTEIN H) - STREPTOCOCCUS PYOGENES.
Scan History
OWL21_1    3  1500 NSINGLE    
OWL26_0 1 1000 NSINGLE
SPTR37_9f 2 100 NSINGLE
Initial Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
QKAKFVLPSTGEQAGL P11000 407 407 -
QSKKSELPETGGEEST P14738 975 975 -
KETKRQLPSTGETANP M24_STRPY 498 498 -
KETKRQLPSTGETANP M5_STRPY 451 451 -
KETKRQLPSTGETANP M6_STRPY 442 442 -
TQQKRTLPSTGETANP ARP4_STRPY 346 346 -

Motif 2 width=21
Element Seqn Id St Int Rpt
FFTAAALTVMATAGVAAVVKR M6_STRPY 458 0 -
FFTAAAATVMVSAGMLALKRK ARP4_STRPY 362 0 -
LLTTVGLVIVAVAGVYFYRTR P11000 423 0 -
NKGMLFGGLFSILGLALLRRN P14738 991 0 -
FFTAAALTVMATAGVAAVVKR M24_STRPY 514 0 -
FFTAAALTVMATAGVAAVVKR M5_STRPY 467 0 -
Final Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
KETKRQLPSTGEAANP Q54703 403 403 -
KETKRQLPSTGEAANP Q54843 488 488 -
KETKRQLPSTGEAANP Q54839 396 396 -
KETKRQLPSTGEAANP Q54837 368 368 -
KETKRQLPSTGETANP M24_STRPY 498 498 -
KETKRQLPSTGETANP M5_STRPY 451 451 -
KETKRQLPSTGETANP M6_STRPY 442 442 -
KETKRQLPSTGETANP Q10372 444 444 -
KETKRQLPSTGETANP Q54511 309 309 -
KETKRQLPSTGETANP Q54719 499 499 -
KETKRQLPSTGETANP Q54835 541 541 -
KETKRQLPSTGETANP SPH_STRPY 336 336 -
KETKRQLPSTGEATNP Q54718 518 518 -
KETKRQLPSTGETANP Q54840 511 511 -
KETKRQLPSTGEATNP Q55098 492 492 -
KETKRQLPSTGEATNP O33631 435 435 -
KETKRQLPSTGEATNP Q05464 444 444 -
KETKRQLPSTGEATNP Q55312 547 547 -
KETKRQLPSTGEATNP Q55246 401 401 -
KETKRQLPSTGEATNP Q00720 552 552 -
TQQKRTLPSTGEAANP Q54901 326 326 -
TQQKRTLPSTGEAANP Q54876 363 363 -
TQQKRTLPSTGETANP Q54841 345 345 -
TQQKRTLPSTGETANP Q53475 338 338 -
TQQKRTLPSTGETANP M49_STRPY 350 350 -
TQQKRTLPSTGETANP M21_STRPY 368 368 -
TQQKRTLPSTGETANP ARP4_STRPY 346 346 -
TQQKRTLPSTGETANP Q54829 347 347 -
KGMRSQLPSTGEAANP M22_STRPY 333 333 -
KGMRSQLPSTGEAANP MX_STRPY 330 330 -
KGMRSQLPSTGEAANP Q54850 319 319 -
KGMRSQLPSTGEAANP Q54851 311 311 -
AQTKRQLPSTGEETTN MRP4_STRPY 348 348 -
AQTKRQLPSTGEETTN Q54860 385 385 -
AQTKRQLPSTGEETTN Q54744 385 385 -
AQTKRQLPSTGEETTN Q53474 348 348 -
AQTKRQLPSTGEETTN P95813 375 375 -
AQTKRQLPSTGEETTN P95810 344 344 -
AQTKRQLPSTGEETTN P95808 385 385 -
TQQKRTLPSTGEAANP Q54555 365 365 -
PQTKRQLPSTGEETTN Q54859 375 375 -
KGMRSQLPSTGEAANP Q54849 326 326 -
KGMRSQLPSTGEAANP Q54746 336 336 -
TQQKRTLPSTGETANP Q54745 374 374 -
AQTKRELPSTGEETTN Q54842 347 347 -
KQDANKLPSTGEATNP Q54071 336 336 -
KQDTNKLPSTGEATNP O33899 334 334 -
TAKAGQLPSTGESANP O33898 494 494 -
TAKAGQLPSTGESANP O68165 494 494 -
KEKAKTLPTTGEKANP Q56212 389 389 -
AKKAATLPTTGEGSNP Q53974 373 373 -
AKKAETLPTTGEGSNP SPG2_STRSP 553 553 -
AKKAETLPTTGEGSNP SPG1_STRSP 408 408 -
AKKAATLPTTGEGSNP Q53975 624 624 -
KGMRSQLPSTGDETNP Q53476 329 329 -
KKDEKKLPSTGETVNP Q55105 399 399 -
NGKGNKLPATGENATP BCA_STRAG 981 981 -
NGKGNKLPATGENATP P72362 1192 1192 -

Motif 2 width=21
Element Seqn Id St Int Rpt
FFTAAALTVMATAGVAAVVKR Q54703 419 0 -
FFTAAALTVMATAGVAAVVKR Q54843 504 0 -
FFTAAALTVMATAGVAAVVKR Q54839 412 0 -
FFTAAALTVMATAGVAAVVKR Q54837 384 0 -
FFTAAALTVMATAGVAAVVKR M24_STRPY 514 0 -
FFTAAALTVMATAGVAAVVKR M5_STRPY 467 0 -
FFTAAALTVMATAGVAAVVKR M6_STRPY 458 0 -
FFTAAALTVMATAGVAAVVKR Q10372 460 0 -
FFTAAALTVMATAGVAAVVKR Q54511 325 0 -
FFTAAALTVMATAGVAAVVKR Q54719 515 0 -
FFTAAALTVMATAGVAAVVKR Q54835 557 0 -
FFTAAALTVMATAGVAAVVKR SPH_STRPY 352 0 -
FFTAAALTVMATAGVAAVVKR Q54718 534 0 -
FFTAAALTVMATAGVAVVKRK Q54840 527 0 -
FFTAAALAVMATAGVAAVVKR Q55098 508 0 -
FFTAAALAVMATAGVAAVVKR O33631 451 0 -
FFTAAAFTVMATAGVAAVVKR Q05464 460 0 -
FFTAAALAVMATAGVAAVAKR Q55312 563 0 -
FFTAAALAVMATAGVAAVAKR Q55246 417 0 -
FFTAAALAVMATAGVAAVAKR Q00720 568 0 -
FFTAAAATVMVSAGMLALKRK Q54901 342 0 -
FFTAAAATVMVSAGMLALKRK Q54876 379 0 -
FFTAAAATVMVSAGMLALKRK Q54841 361 0 -
FFTAAAATVMVSAGMLALKRK Q53475 354 0 -
FFTAAAATVMVSAGMLALKRK M49_STRPY 366 0 -
FFTAAAATVMVSAGMLALKRK M21_STRPY 384 0 -
FFTAAAATVMVSAGMLALKRK ARP4_STRPY 362 0 -
FFTAAAAIVMVSAGMLALKRK Q54829 363 0 -
FFTAAAATVMVSAGMLALKRK M22_STRPY 349 0 -
FFTAAAATVMVSAGMLALKRK MX_STRPY 346 0 -
FFTAAAATVMVSAGMLALKRK Q54850 335 0 -
FFTAAAATVMVSAGMLALKRK Q54851 327 0 -
FFTAAALTVIASAGVLALKRK MRP4_STRPY 365 1 -
FFTAAALTVIASAGVLALKRK Q54860 402 1 -
FFTAAALTVIASAGVLALKRK Q54744 402 1 -
FFTAAALTVIASAGVLALKRK Q53474 365 1 -
FFTAAALTVIASAGVLALKRK P95813 392 1 -
FFTAAALTVIASAGVLALKRK P95810 361 1 -
FFTAAALTVIASAGVLALKRK P95808 402 1 -
FFTAAAATVMDTAAMLALKRK Q54555 381 0 -
FFTAAALTVIASAGVLALKRK Q54859 392 1 -
FFTEAAATVMVSAGMLALKRK Q54849 342 0 -
FFTAAAATVMVSAGMLTLKRK Q54746 352 0 -
FFTEEAATVMVSAGMLALKRK Q54745 390 0 -
FFTAAALAVIASAGVFALKRK Q54842 364 1 -
FFTAAALAVMAGAGVAAVSTR Q54071 352 0 -
FFTAAALAVMAGAGVAAVSTR O33899 350 0 -
FFTIAALTVIAGAGMAVVSPK O33898 510 0 -
FFTIAALTVIAGAGMAVVSPK O68165 510 0 -
FFTAAALAIMAGAGALAVTSK Q56212 405 0 -
FFTAAALAVMAGAGALAVASK Q53974 389 0 -
FFTAAALAVMAGAGALAVASK SPG2_STRSP 569 0 -
FFTAAALAVMAGAGALAVASK SPG1_STRSP 424 0 -
FFTAAALAVMAGAGALAVASK Q53975 640 0 -
PFFTAAATVMVSAGMLALKRK Q53476 344 -1 -
FFTAAGMAGMATAGVVAVGKR Q55105 415 0 -
FFNVAALTIISSVGLLSVSKK BCA_STRAG 997 0 -
FFNVVALTIMSSVGLLSVSKK P72362 1208 0 -