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PR00009

Identifier
EGFTGF  [View Relations]  [View Alignment]  
Accession
PR00009
No. of Motifs
4
Creation Date
03-SEP-1991  (UPDATE 14-JUN-1999)
Title
Type I EGF signature
Database References

PROSITE; PS00022 EGF
BLOCKS; BL00022
PFAM; PF00008 EGF
INTERPRO; IPR001336
PDB; 1EGF
SCOP; 1EGF
CATH; 1EGF
Literature References
1. CAMPBELL, I.D., BARON, M., COOKE, R.M., DUDGEON, T.J., FALLON, A.,
HARVEY, T.S. AND TAPPIN, M.J.
Structure-function relationship in Epidermal Growth Factor (EGF)
and Transforming Growth Factor-alpha (TGF-alpha).
BIOCHEM.PHARMACOL. 40(1) 35-40 (1990).

Documentation
Epidermal growth factors and transforming growth factors belong to a
general class of proteins that share a repeat pattern involving a number 
of conserved Cys residues. Growth factors are involved in cell recognition 
and division [1]: the repeating pattern, especially of cysteines (the 
so-called EGF repeat), is thought to be important to the 3D structure of 
the proteins, and hence its recognition by receptors and other molecules.
 
The EGF motif is found frequently in nature, particularly in extracellular 
proteins. The spacing of conserved cysteines, however, is variable, and for
this reason 2 further EGF, or EGF-like, motifs, have been classified - see 
signatures EGFBLOOD and EGFLAMININ. 
 
EGFTGF is a 4-element fingerprint that provides a signature for type I 
EGF repeats. The fingerprint was derived from an initial alignment of 9 
sequences (after Campbell et al., [1]): the motifs include 6 conserved
cysteines believed to be involved in disulphide bond formation, motifs 3
and 4 spanning the region encoded by PROSITE pattern EGF (PS00022). Four
iterations on OWL12.0 were required to reach convergence, at which point a 
true set comprising 12 sequences was identified: these included epidermal,
transforming and viral growth factors. 
 
An update on SPTR37_9f identified a true set of 22 sequences, and 21
partial matches.
Summary Information
  22 codes involving  4 elements
3 codes involving 3 elements
18 codes involving 2 elements
Composite Feature Index
422222222
33033
21021212
1234
True Positives
BTC_HUMAN     BTC_MOUSE     EGF_HUMAN     EGF_MOUSE     
EGF_RAT GRFA_MYXVL GRFA_SFVKA GRFA_VACCC
GRFA_VACCV GRFA_VARV NOTC_BRARE O14944
O57166 Q15577 Q61521 Q86607
Q89066 Q89756 TGFA_HUMAN TGFA_MOUSE
TGFA_PIG TGFA_RAT
True Positive Partials
Codes involving 3 elements
NOTC_XENLA NTC1_MOUSE NTC1_RAT
Codes involving 2 elements
FBN1_BOVIN FBN1_HUMAN FBN1_MOUSE NOTC_DROME
NTC3_MOUSE NTC4_MOUSE O00306 O16004
O35442 O61240 O88281 O88840
Q19350 Q21340 Q21756 Q25253
Q99940 YREC_VIBCH
Sequence Titles
BTC_HUMAN   BETACELLULIN PRECURSOR (BTC) - HOMO SAPIENS (HUMAN). 
BTC_MOUSE BETACELLULIN PRECURSOR (BTC) - MUS MUSCULUS (MOUSE).
EGF_HUMAN PRO-EPIDERMAL GROWTH FACTOR PRECURSOR (EGF) [CONTAINS: EPIDERMAL GROWTH FACTOR (UROGASTRONE)] - HOMO SAPIENS (HUMAN).
EGF_MOUSE PRO-EPIDERMAL GROWTH FACTOR PRECURSOR (EGF) [CONTAINS: EPIDERMAL GROWTH FACTOR] - MUS MUSCULUS (MOUSE).
EGF_RAT PRO-EPIDERMAL GROWTH FACTOR PRECURSOR (EGF) [CONTAINS: EPIDERMAL GROWTH FACTOR] - RATTUS NORVEGICUS (RAT).
GRFA_MYXVL GROWTH FACTOR (MGF) - MYXOMA VIRUS (STRAIN LAUSANNE).
GRFA_SFVKA GROWTH FACTOR - SHOPE FIBROMA VIRUS (STRAIN KASZA) (SFV).
GRFA_VACCC GROWTH FACTOR - VACCINIA VIRUS (STRAIN COPENHAGEN).
GRFA_VACCV GROWTH FACTOR - VACCINIA VIRUS (STRAIN WR).
GRFA_VARV GROWTH FACTOR - VARIOLA VIRUS.
NOTC_BRARE NEUROGENIC LOCUS NOTCH HOMOLOG PROTEIN PRECURSOR - Brachydanio rerio (Zebrafish) (Zebra danio).
O14944 EPIREGULIN - HOMO SAPIENS (HUMAN).
O57166 GROWTH FACTOR PROTEIN - VACCINIA VIRUS.
Q15577 TRANSFORMING GROWTH FACTOR-ALPHA PRECURSOR - HOMO SAPIENS (HUMAN).
Q61521 EPIREGULIN - MUS MUSCULUS (MOUSE).
Q86607 GROWTH FACTOR - VACCINIA VIRUS.
Q89066 GARCIA-1966 LEFT NEAR-TERMINAL REGION - VARIOLA VIRUS.
Q89756 HOMOLOG OF VACCINIA VIRUS CDS C11R - VARIOLA VIRUS.
TGFA_HUMAN TRANSFORMING GROWTH FACTOR ALPHA PRECURSOR (TGF-ALPHA) (EGF-LIKE TGF) (ETGF) (TGF TYPE 1) - HOMO SAPIENS (HUMAN).
TGFA_MOUSE TRANSFORMING GROWTH FACTOR ALPHA PRECURSOR (TGF-ALPHA) (EGF-LIKE TGF) (ETGF) (TGF TYPE 1) - MUS MUSCULUS (MOUSE).
TGFA_PIG TRANSFORMING GROWTH FACTOR ALPHA PRECURSOR (TGF-ALPHA) (EGF-LIKE TGF) (ETGF) (TGF TYPE 1) - SUS SCROFA (PIG).
TGFA_RAT TRANSFORMING GROWTH FACTOR ALPHA PRECURSOR (TGF-ALPHA) (EGF-LIKE TGF) (ETGF) (TGF TYPE 1) - RATTUS NORVEGICUS (RAT).

NOTC_XENLA NEUROGENIC LOCUS NOTCH PROTEIN HOMOLOG PRECURSOR (XOTCH PROTEIN) - Xenopus laevis (African clawed frog).
NTC1_MOUSE NEUROGENIC LOCUS NOTCH HOMOLOG PROTEIN 1 PRECURSOR (MOTCH PROTEIN) - Mus musculus (Mouse).
NTC1_RAT NEUROGENIC LOCUS NOTCH HOMOLOG PROTEIN 1 PRECURSOR - Rattus norvegicus (Rat).

FBN1_BOVIN FIBRILLIN 1 PRECURSOR (MP340) - BOS TAURUS (BOVINE).
FBN1_HUMAN FIBRILLIN 1 PRECURSOR - HOMO SAPIENS (HUMAN).
FBN1_MOUSE FIBRILLIN 1 PRECURSOR - MUS MUSCULUS (MOUSE).
NOTC_DROME NEUROGENIC LOCUS NOTCH PROTEIN PRECURSOR - Drosophila melanogaster (Fruit fly).
NTC3_MOUSE NEUROGENIC LOCUS NOTCH 3 PROTEIN - Mus musculus (Mouse).
NTC4_MOUSE NEUROGENIC LOCUS NOTCH HOMOLOG PROTEIN 4 PRECURSOR (TRANSFORMING PROTEIN INT-3) - Mus musculus (Mouse).
O00306 NOTCH4 - Homo sapiens (Human).
O16004 NOTCH HOMOLOG - Lytechinus variegatus (Sea urchin).
O35442 NOTCH4 - Mus musculus (Mouse).
O61240 HRNOTCH PROTEIN - Halocynthia roretzi (Sea squirt).
O88281 MEGF6 - RATTUS NORVEGICUS (RAT).
O88840 MUTANT FIBRILLIN-1 - MUS MUSCULUS (MOUSE).
Q19350 SIMILAR TO EGF-LIKE REPEATS. NCBI GI: 1125776 - CAENORHABDITIS ELEGANS.
Q21340 K08E5.3 PROTEIN.
Q21756 HYPOTHETICAL 39.1 KD PROTEIN - CAENORHABDITIS ELEGANS.
Q25253 NOTCH HOMOLOG SCALLOPED WINGS (SCL) - Lucilia cuprina (Greenbottle fly) (Australian sheep blowfly).
Q99940 NOTCH4 - Homo sapiens (Human).
YREC_VIBCH HYPOTHETICAL 41.3 KD PROTEIN - VIBRIO CHOLERAE.
Scan History
OWL12_0    4  50   NSINGLE    
OWL17_1 1 25 NSINGLE
OWL18_0 1 45 NSINGLE
OWL19_1 1 75 NSINGLE
OWL26_0 1 300 NSINGLE
SPTR37_9f 2 200 NSINGLE
Initial Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
PAIRLCGPEGDGYCLH GRFA_VACCV 40 40 -
SHFNKCPDSHTQYCFH TGFA_RAT 41 41 -
SHFNDCPDSHTQFCFH TGFA_HUMAN 42 42 -
KHVKVCNHDYENYCLN GRFA_SHFVK 28 28 -
KRIKLCNDDYKNYCLN GRFA_MYXV 32 32 -
NSYPGCPSSYDGYCLN EGF_MOUSE 977 977 -
NSDSECPLSHDGYCLH EGF_HUMAN 971 971 -
NSNTGCPPSYDGYCLN EGF_RAT 1 1 -
PAIRLCGPEGDGYCLH GRFA_VACCC 40 40 -

Motif 2 width=8
Element Seqn Id St Int Rpt
GTCRFLVQ TGFA_HUMAN 58 0 -
GTCFTVAL GRFA_MYXV 49 1 -
GTCFTIAL GRFA_SHFVK 45 1 -
GDCIHARD GRFA_VACCC 56 0 -
GDCIHARD GRFA_VACCV 56 0 -
GTCRFLVQ TGFA_RAT 57 0 -
GVCMYVES EGF_RAT 18 1 -
GVCMYIEA EGF_HUMAN 988 1 -
GVCMHIES EGF_MOUSE 994 1 -

Motif 3 width=12
Element Seqn Id St Int Rpt
SLNPFCACHINY GRFA_MYXV 60 3 -
IDGMYCRCSHGY GRFA_VACCC 64 0 -
IDGMYCRCSHGY GRFA_VACCV 64 0 -
EEKPACVCHSGY TGFA_RAT 65 0 -
EDKPACVCHSGY TGFA_HUMAN 66 0 -
VDRYVCNCVIGY EGF_RAT 26 0 -
LDKYACNCVVGY EGF_HUMAN 996 0 -
LDSYTCNCVIGY EGF_MOUSE 1002 0 -
SITPFCVCRINY GRFA_SHFVK 56 3 -

Motif 4 width=10
Element Seqn Id St Int Rpt
TGIRCQHVVL GRFA_VACCV 76 0 -
IGERCQHRDL EGF_RAT 38 0 -
IGERCQYRDL EGF_HUMAN 1008 0 -
SGDRCQTRDL EGF_MOUSE 1014 0 -
VGVRCEHADL TGFA_RAT 77 0 -
TGIRCQHVVL GRFA_VACCC 76 0 -
EGSRCQFINL GRFA_SHFVK 68 0 -
VGSRCQFINL GRFA_MYXV 72 0 -
VGARCEHADL TGFA_HUMAN 78 0 -
Final Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
PAIRLCGPEGDGYCLH GRFA_VACCC 40 40 -
PAIRLCGPEGDGYCLH GRFA_VACCV 40 40 -
PAIRLCGPEGDGYCLH O57166 40 40 -
PAIRLCGPEGDGYCLH Q86607 40 40 -
PAIRLCGPEGNGYCFH Q89066 40 40 -
PAIRLCGPEGNGYCFH Q89756 40 40 -
PAIRLCGPEGDRYCFH GRFA_VARV 40 40 -
SHFNKCPDSHTQYCFH TGFA_MOUSE 41 41 -
SHFNKCPDSHTQYCFH TGFA_RAT 41 41 -
SHFNDCPDSHTQFCFH TGFA_HUMAN 42 42 -
SHFNDCPDSHSQFCFH TGFA_PIG 42 42 -
SHFNDCPDSHTQFCFH Q15577 42 42 -
VSITKCSSDMNGYCLH O14944 63 63 -
VQITKCSSDMDGYCLH Q61521 56 56 -
THFSRCPKQYKHYCIH BTC_MOUSE 64 64 -
NSNTGCPPSYDGYCLN EGF_RAT 1 1 -
NSDSECPLSHDGYCLH EGF_HUMAN 971 971 -
GHFSRCPKQYKHYCIK BTC_HUMAN 64 64 -
NSYPGCPSSYDGYCLN EGF_MOUSE 977 977 -
KRIKLCNDDYKNYCLN GRFA_MYXVL 32 32 -
KAICTCPPGYTGSACN NOTC_BRARE 394 394 -
KHVKVCNHDYENYCLN GRFA_SFVKA 28 28 -

Motif 2 width=8
Element Seqn Id St Int Rpt
GDCIHARD GRFA_VACCC 56 0 -
GDCIHARD GRFA_VACCV 56 0 -
GDCIHARD O57166 56 0 -
GDCIHARD Q86607 56 0 -
GICIHARD Q89066 56 0 -
GICIHARD Q89756 56 0 -
GICIHARD GRFA_VARV 56 0 -
GTCRFLVQ TGFA_MOUSE 57 0 -
GTCRFLVQ TGFA_RAT 57 0 -
GTCRFLVQ TGFA_HUMAN 58 0 -
GTCRFLVQ TGFA_PIG 58 0 -
ATCRFLVH Q15577 58 0 -
GQCIYLVD O14944 79 0 -
GQCIYLVD Q61521 72 0 -
GRCRFVVD BTC_MOUSE 80 0 -
GVCMYVES EGF_RAT 18 1 -
GVCMYIEA EGF_HUMAN 988 1 -
GRCRFVVA BTC_HUMAN 80 0 -
GVCMHIES EGF_MOUSE 994 1 -
GTCFTVAL GRFA_MYXVL 49 1 -
GVCRESED NOTC_BRARE 840 430 -
GTCFTIAL GRFA_SFVKA 45 1 -

Motif 3 width=12
Element Seqn Id St Int Rpt
IDGMYCRCSHGY GRFA_VACCC 64 0 -
IDGMYCRCSHGY GRFA_VACCV 64 0 -
IDGMYCRCSHGY O57166 64 0 -
IDGMYCRCSHGY Q86607 64 0 -
IDGMYCRCSHGY Q89066 64 0 -
IDGMYCRCSHGY Q89756 64 0 -
IDGMYCRCSHGY GRFA_VARV 64 0 -
EEKPACVCHSGY TGFA_MOUSE 65 0 -
EEKPACVCHSGY TGFA_RAT 65 0 -
EDKPACVCHSGY TGFA_HUMAN 66 0 -
EDKPACVCHSGY TGFA_PIG 66 0 -
EDKPACVCHSGY Q15577 66 0 -
MSQNYCRCEVGY O14944 87 0 -
MREKFCRCEVGY Q61521 80 0 -
EQTPSCICEKGY BTC_MOUSE 88 0 -
VDRYVCNCVIGY EGF_RAT 26 0 -
LDKYACNCVVGY EGF_HUMAN 996 0 -
EQTPSCVCDEGY BTC_HUMAN 88 0 -
LDSYTCNCVIGY EGF_MOUSE 1002 0 -
SLNPFCACHINY GRFA_MYXVL 60 3 -
LGGYSCECVPGY NOTC_BRARE 1162 314 -
SITPFCVCRINY GRFA_SFVKA 56 3 -

Motif 4 width=10
Element Seqn Id St Int Rpt
TGIRCQHVVL GRFA_VACCC 76 0 -
TGIRCQHVVL GRFA_VACCV 76 0 -
TGIRCQHVVL O57166 76 0 -
TGIRCQHVVL Q86607 76 0 -
TGIRCQHVVL Q89066 76 0 -
TGIRCQHVVL Q89756 76 0 -
TGIRCQHVVL GRFA_VARV 76 0 -
VGVRCEHADL TGFA_MOUSE 77 0 -
VGVRCEHADL TGFA_RAT 77 0 -
VGARCEHADL TGFA_HUMAN 78 0 -
VGARCEHADL TGFA_PIG 78 0 -
VGARCEHADL Q15577 78 0 -
TGVRCEHFFL O14944 99 0 -
TGLRCEHFFL Q61521 92 0 -
FGARCERVDL BTC_MOUSE 100 0 -
IGERCQHRDL EGF_RAT 38 0 -
IGERCQYRDL EGF_HUMAN 1008 0 -
IGARCERVDL BTC_HUMAN 100 0 -
SGDRCQTRDL EGF_MOUSE 1014 0 -
VGSRCQFINL GRFA_MYXVL 72 0 -
VGERCEGDVN NOTC_BRARE 1258 84 -
EGSRCQFINL GRFA_SFVKA 68 0 -