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PR00006

Identifier
COFILIN  [View Relations]  [View Alignment]  
Accession
PR00006
No. of Motifs
5
Creation Date
24-JUN-1993  (UPDATE 14-JUN-1999)
Title
Cofilin/destrin family signature
Database References

PROSITE; PS00325 COFILIN_TROPOMYOSIN
BLOCKS; BL00325
PFAM; PF00241 cofilin_ADF
INTERPRO; IPR002108
Literature References
1. ABE, H., ENDO, T., YAMAMOTO, K. AND OBINATA, T.
Sequence of cDNA's encoding actin depolymerizing factor and cofilin of
embryonic chicken skeletal muscle - 2 functionally distinct actin regulatory
proteins exhibit high structural homology.
BIOCHEMISTRY 29(32) 7420-7425 (1990).
 
2. OGAWA, K., TASHIMA, M., YUMOTO, Y., OKUDA, T., SAWADA, H., OKUMA, M. 
AND MARUYAMA, Y.
Coding sequence of human placenta Cofilin cDNA.
NUCLEIC ACIDS RES. 18 7169-7169 (1990).
 
3. MORIYAMA, K., NISHIDA, E., YONEZAWA, N., SAKAI, H., MATSUMOTO, S., 
IIDA, K. AND YAHARA, I.
Destrin, a mammalian actin depolymerizing protein, is closely related to
cofilin - Cloning and expression of porcine brain destrin cDNA.
J.BIOL.CHEM. 265(10) 5768-5773 (1990).
 
4. ADAMS, M.E., MINAMIDE, L.S., DUESTER, G. AND BAMBURG, J.R.
Nucleotide sequence and expression of a cDNA encoding chick brain actin
depolymerizing factor.
BIOCHEMISTRY 29(32) 7414-7420 (1990).

Documentation
Actin is a major component of skeletal muscle thin filaments and rapidly 
polymerises in vitro when purified, although it is unclear how this is
regulated in developing muscle cells [1]. Three regulatory proteins have
been identified in embryonic muscle, where actin is largely unpolymerised,
one of which is cofilin. Cofilin is an actin-modulating protein present in 
brain tissue but not muscle cells, and forms intracellular actin/cofilin
rods in cultured cells exposed to heat shock [2]. It also forms a 1:1
complex with G-actin (monomers) to inhibit polymerisation, and rapidly
depolymerises filamentous actin (F-actin).
 
Destrin is a protein that also depolymerises F-actin and is very similar
to cofilin, but by contrast is not pH sensitive [3]. Destrin is able to
interact directly with F-actin protomers and remove actin monomers from 
the entire region of F-actin. The 2 proteins differ in their interaction 
with actin, although they are similar in sequence, suggesting that subtle
differences in amino acid composition, possibly in the C-terminal region,
are responsible for their functional distinction. Both proteins contain the
sequence DAIKKK, also found at the C-terminus of tropomyosin, which is
thought to be involved in actin binding [4].
 
COFILIN is a 5-element fingerprint that provides a signature for the 
cofilin/destrin family. The fingerprint was derived from an initial 
alignment of 4 sequences: motif 4 includes the F-actin binding domain 
encoded by PROSITE pattern COFILIN_TROPOMYOSIN (PS00325). Two iterations
on OWL20.0 were required to reach convergence, at which point a true set
comprising 6 sequences was identified. Three partial matches were also
found: an actophorin and 2 actin depolymerising factor fragments.
 
An update on SPTR37_9f identified a true set of 10 sequences, and 1
partial match.
Summary Information
  10 codes involving  5 elements
0 codes involving 4 elements
0 codes involving 3 elements
1 codes involving 2 elements
Composite Feature Index
51010101010
400000
300000
200110
12345
True Positives
COF1_HUMAN    COF1_MOUSE    COF1_PIG      COF1_RAT      
COF1_XENLA COF2_MOUSE COF2_XENLA COFI_CHICK
DEST_CHICK DEST_HUMAN
True Positive Partials
Codes involving 2 elements
ACTP_ACACA
Sequence Titles
COF1_HUMAN  COFILIN, NON-MUSCLE ISOFORM - HOMO SAPIENS (HUMAN). 
COF1_MOUSE COFILIN, NON-MUSCLE ISOFORM - MUS MUSCULUS (MOUSE).
COF1_PIG COFILIN, NON-MUSCLE ISOFORM - SUS SCROFA (PIG).
COF1_RAT COFILIN, NON-MUSCLE ISOFORM - RATTUS NORVEGICUS (RAT).
COF1_XENLA COFILIN 1 - XENOPUS LAEVIS (AFRICAN CLAWED FROG).
COF2_MOUSE COFILIN, MUSCLE ISOFORM - MUS MUSCULUS (MOUSE).
COF2_XENLA COFILIN 2 - XENOPUS LAEVIS (AFRICAN CLAWED FROG).
COFI_CHICK COFILIN - GALLUS GALLUS (CHICKEN).
DEST_CHICK DESTRIN (ACTIN DEPOLYMERIZING FACTOR) (ADF) - GALLUS GALLUS (CHICKEN).
DEST_HUMAN DESTRIN (ACTIN DEPOLYMERIZING FACTOR) (ADF) - HOMO SAPIENS (HUMAN), AND SUS SCROFA (PIG).

ACTP_ACACA ACTOPHORIN - ACANTHAMOEBA CASTELLANII (AMOEBA).
Scan History
OWL20_0    2  100  NSINGLE    
OWL26_0 1 250 NSINGLE
SPTR37_9f 2 50 NSINGLE
Initial Motifs
Motif 1  width=25
Element Seqn Id St Int Rpt
DMKVRKSSTPEEVKKRKKAVLFCLS COFI_PIG 17 17 -
DMKVRKCSTPEEIKKRKKAVIFCLS DEST_PIG 17 17 -
DMKVRKSSTPEEIKKRKKAVLFCLS COFI_CHICK 17 17 -
DMKVRKCSTPEEVKKRKKAVIFCLS DEST_CHICK 17 17 -

Motif 2 width=21
Element Seqn Id St Int Rpt
DCRYALYDASFETKESRKEEL DEST_PIG 79 37 -
DCRYALYDATYETKESKKEDL COFI_CHICK 79 37 -
DCRYALYDASFETKESKKEEL DEST_CHICK 79 37 -
DCRYALYDATYETKESKKEDL COFI_PIG 79 37 -

Motif 3 width=22
Element Seqn Id St Int Rpt
MFFLWAPELAPLKSKMIYASSK DEST_PIG 100 0 -
VFIFWAPECAPLKSKMIYASSK COFI_PIG 100 0 -
MFFLWAPEQAPLKSKMIYASSK DEST_CHICK 100 0 -
VFIFWAPESAPLKSKMIYASSK COFI_CHICK 100 0 -

Motif 4 width=17
Element Seqn Id St Int Rpt
DAIKKKFQGIKHECQAN DEST_PIG 122 0 -
DAIKKKFQGIKHECQAN DEST_CHICK 122 0 -
DAIKKKFTGIKHEWQVN COFI_CHICK 122 0 -
DAIKKKLTGIKHELQAN COFI_PIG 122 0 -

Motif 5 width=13
Element Seqn Id St Int Rpt
LGGNVVVSLEGKP COFI_CHICK 153 14 -
LGGSLIVAFEGCP DEST_PIG 152 13 -
LGGSAVISLEGKP COFI_PIG 153 14 -
LGGSLVVAFEGSP DEST_CHICK 152 13 -
Final Motifs
Motif 1  width=25
Element Seqn Id St Int Rpt
DMKVRKSSTPEEVKKRKKAVLFCLS COF1_RAT 17 17 -
DMKVRKSSTPEEVKKRKKAVLFCLS COF1_MOUSE 17 17 -
DMKVRKSSTPEEVKKRKKAVLFCLS COF1_PIG 17 17 -
DMKVRKSSTQEEIKKRKKAVLFCLS COF2_MOUSE 17 17 -
DMKVRKSSTPEEIKKRKKAVLFCLS COFI_CHICK 17 17 -
DMKVRKCSTPEEVKKRKKAVIFCLS DEST_CHICK 17 17 -
DMKVRHQLSPEEAKKRKKAVIFCLS COF2_XENLA 17 17 -
EMKVRHQLSPEDAKKRKKAVVFCLS COF1_XENLA 17 17 -
DMKVRKSSTPEEVKKRKKAVLFCLS COF1_HUMAN 17 17 -
DMKVRKCSTPEEIKKRKKAVIFCLS DEST_HUMAN 17 17 -

Motif 2 width=21
Element Seqn Id St Int Rpt
DCRYALYDALYETKETKKEDL COF1_XENLA 79 37 -
DCRYALYDATYETKESKKEDL COF1_HUMAN 79 37 -
DCRYALYDATYETKESKKEDL COF1_RAT 79 37 -
DCRYALYDATYETKESKKEDL COF1_MOUSE 79 37 -
DCRYALYDATYETKESKKEDL COF1_PIG 79 37 -
DCRYALYDATYETKESKKEDL COF2_MOUSE 79 37 -
DCRYALYDATYETKESKKEDL COFI_CHICK 79 37 -
DCRYALYDASFETKESKKEEL DEST_CHICK 79 37 -
DCRYALYDALYETKETKKEDL COF2_XENLA 79 37 -
DCRYALYDASFETKESRKEEL DEST_HUMAN 79 37 -

Motif 3 width=22
Element Seqn Id St Int Rpt
VFIFWAPESAPLKSKMIYASSK COF2_MOUSE 100 0 -
VFIFWAPESAPLKSKMIYASSK COF1_HUMAN 100 0 -
VFIFWAPESAPLKSKMIYASSK COF1_RAT 100 0 -
VFIFWAPENAPLKSKMIYASSK COF1_MOUSE 100 0 -
VFIFWAPECAPLKSKMIYASSK COF1_PIG 100 0 -
VFIFWAPESAPLKSKMIYASSK COFI_CHICK 100 0 -
MFFLWAPEQAPLKSKMIYASSK DEST_CHICK 100 0 -
VFVFWAPEEASLKSKMIYASSK COF2_XENLA 100 0 -
VFVFWAPEEASLKSKMIYASSK COF1_XENLA 100 0 -
MFFLWAPELAPLKSKMIYASSK DEST_HUMAN 100 0 -

Motif 4 width=17
Element Seqn Id St Int Rpt
DAIKKKLTGIKHELQAN COF1_HUMAN 122 0 -
DAIKKKLTGIKHELQAN COF1_RAT 122 0 -
DAIKKKLTGIKHELQAN COF1_MOUSE 122 0 -
DAIKKKLTGIKHELQAN COF1_PIG 122 0 -
DAIKKKFTGIKHEWQVN COF2_MOUSE 122 0 -
DAIKKKFTGIKHEWQVN COFI_CHICK 122 0 -
DAIKKKFQGIKHECQAN DEST_CHICK 122 0 -
DAIRKRFTGIKHEWQTN COF2_XENLA 122 0 -
DAIKKRLPGIKHEWQIN COF1_XENLA 122 0 -
DAIKKKFQGIKHECQAN DEST_HUMAN 122 0 -

Motif 5 width=13
Element Seqn Id St Int Rpt
LGGNTVVSLEGKS COF1_XENLA 153 14 -
LGGSAVISLEGKP COF1_HUMAN 153 14 -
LGGSAVISLEGKP COF1_RAT 153 14 -
LGGSAVISLEGKP COF1_MOUSE 153 14 -
LGGSAVISLEGKP COF1_PIG 153 14 -
LGGSVVVSLEGKP COF2_MOUSE 153 14 -
LGGNVVVSLEGKP COFI_CHICK 153 14 -
LGGSLVVAFEGSP DEST_CHICK 152 13 -
LGGNTVVSLEGKS COF2_XENLA 153 14 -
LGGSLIVAFEGCP DEST_HUMAN 152 13 -