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PR01910

Identifier
ADSPHPHTASEB  [View Relations]  [View Alignment]  
Accession
PR01910
No. of Motifs
4
Creation Date
11-MAY-2006
Title
Atypical dual specificity phosphatase subfamily B signature
Database References
PRINTS; PR00700 PRTYPHPHTASE; PR01764 MAPKPHPHTASE; PR01908 ADSPHPHTASE

INTERPRO; IPR000340; IPR000387
PDB; 2ESB
Literature References
1. ISHIBASHI, T., BOTTARO, D.P., CHAN, A., MIKI, T. AND AARONSON, S.A. 
Expression cloning of a human dual-specificity phosphatase. 
PROC.NATL.ACAD.SCI.U.S.A. 89 12170-12174 (1992). 
 
2. YUVANIYAMA, J., DENU, J.M., DIXON, J.E. AND SAPER, M.A. 
Crystal structure of the dual specificity protein phosphatase VHR. 
SCIENCE 272 1328-1331 (1996). 
 
3. TODD, J.L., TANNER, K.G. AND DENU, J.M. 
Extracellular regulated kinases (ERK) 1 and ERK2 are authentic substrates 
for the dual-specificity protein-tyrosine phosphatase VHR. A novel role in 
down-regulating the ERK pathway.
J.BIOL.CHEM. 274(19) 13271-80 (1999).
 
4. TODD, J.L., RIGAS, J.D., RAFTY, L.A. AND DENU, J.M. 
Dual-specificity protein tyrosine phosphatase VHR down-regulates c-Jun 
N-terminal kinase (JNK).
ONCOGENE 21(16) 2573-83 (2002).
 
5. VASUDEVAN, S.A., SKOKO, J., WANG, K., BURLINGAME, S.M., PATEL, P.N., 
LAZO, J.S., NUCHTERN, J.G. AND YANG, J. 
MKP-8, a novel MAPK phosphatase that inhibits p38 kinase.
BIOCHEM.BIOPHYS.RES.COMMUN. 330(2) 511-8 (2005).
 
6. RAHMOUNI, S., CERIGNOLI, F., ALONSO, A., TSUTJI, T., HENKENS, R., 
ZHU, C., LOUIS-DIT-SULLY, C., MOUTSCHEN, M., JIANG, W. AND MUSTELIN, T. 
Loss of the VHR dual-specific phosphatase causes cell-cycle arrest and
senescence.
NAT.CELL BIOL. 8(5) 524-31 (2006). 

Documentation
Dual Specificity Phosphatases (DUSPs) are members of the superfamily of 
Protein Tyrosine Phosphatases (PTPs). They remove the phosphate group from
both phospho-tyrosine and phospho-serine/threonine residues. Many of these
proteins have been related to the MAP kinase signalling pathway and hence they
are also termed MAP kinase phosphatases (MKPs). A group of DUSPs share a high
degree of similarity with the MKP subgroup, but lack the N-terminal regulatory
domain, which provides the substrate specificity towards the MAP kinases.
These proteins form a heterogeneous group and have in common the presence of
a single catalytic PTP domain. They are small-sized and have been named Low
Molecular Weight-DUSPs (LMW-DUSPs) or Atypical-DUSPs. VHR was the first
characterised member of this subfamily; its crystal structure is known [1,2].
 
The functions of many of the members of this group remain unknown, although 
some have been related to regulation of MAP kinase pathways [3-5]. VHR has
also been related to the control of cell-senescence [6]. On the basis of
sequence similarity, this family can be subdivided into two groups, for
convenience termed A and B. Each of these subgroups is characterised by its
own distinctive set of motifs, the functions of which are as yet unknown. 
 
ADSPHPHTASEB is a 4-element fingerprint that provides a signature for the
atypical dual specificity phosphatase subfamily B. The fingerprint was derived
from an initial alignment of 11 sequences: the motifs were drawn from conserved
regions spanning the N-terminal portion of the alignment, avoiding the catalytic
PTP domain, which is conserved in the PTP superfamily. Two iterations on
SPTR49_32f were required to reach convergence, at which point a true set comprising
21 sequences was identified. 
Summary Information
21 codes involving  4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
421212121
30000
20000
1234
True Positives
DUS14_HUMAN   DUS14_MOUSE   DUS18_BOVIN   DUS18_HUMAN   
DUS18_MOUSE DUS18_PONPY DUS18_RAT DUS21_HUMAN
Q25DK0_MACFA Q32KN1_BOVIN Q3KQ00_XENLA Q3TLZ7_MOUSE
Q4R3T3_MACFA Q5PQ70_XENLA Q5SV91_MOUSE Q5XJN5_BRARE
Q6FI36_HUMAN Q6GM01_XENLA Q7TPY1_MOUSE Q9D9D8_MOUSE
Q9Y346_HUMAN
Sequence Titles
DUS14_HUMAN Dual specificity protein phosphatase 14 (EC 3.1.3.48) (EC 3.1.3.16) (Mitogen-activated protein kinase phosphatase 6) (MAP kinase phosphatase 6) (MKP-6) (MKP-1-like protein tyrosine phosphatase) (MKP- L) - Homo sapiens (Human). 
DUS14_MOUSE Dual specificity protein phosphatase 14 (EC 3.1.3.48) (EC 3.1.3.16) (Mitogen-activated protein kinase phosphatase 6) (MAP kinase phosphatase 6) (MKP-6) - Mus musculus (Mouse).
DUS18_BOVIN Dual specificity protein phosphatase 18 (EC 3.1.3.48) (EC 3.1.3.16) - Bos taurus (Bovine).
DUS18_HUMAN Dual specificity protein phosphatase 18 (EC 3.1.3.48) (EC 3.1.3.16) (Low molecular weight dual specificity phosphatase 20) - Homo sapiens (Human).
DUS18_MOUSE Dual specificity protein phosphatase 18 (EC 3.1.3.48) (EC 3.1.3.16) - Mus musculus (Mouse).
DUS18_PONPY Dual specificity protein phosphatase 18 (EC 3.1.3.48) (EC 3.1.3.16) - Pongo pygmaeus (Orangutan).
DUS18_RAT Dual specificity protein phosphatase 18 (EC 3.1.3.48) (EC 3.1.3.16) - Rattus norvegicus (Rat).
DUS21_HUMAN Dual specificity protein phosphatase 21 (EC 3.1.3.48) (EC 3.1.3.16) (Low molecular weight dual specificity phosphatase 21) - Homo sapiens (Human).
Q25DK0_MACFA Brain cDNA clone: QtrA-16417, similar to human dual specificity phosphatase 14 (DUSP14), - Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey).
Q32KN1_BOVIN Hypothetical protein DUSP18 - Bos taurus (Bovine).
Q3KQ00_XENLA Hypothetical protein - Xenopus laevis (African clawed frog).
Q3TLZ7_MOUSE Mammary gland RCB-0526 Jyg-MC(A) cDNA, RIKEN full-length enriched library, clone:G830020J01 product:dual specificity phosphatase 14, full insert sequence - Mus musculus (Mouse).
Q4R3T3_MACFA Testis cDNA clone: QtsA-14359, similar to human dual specificity phosphatase 18 (DUSP18), - Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey).
Q5PQ70_XENLA LOC495963 protein - Xenopus laevis (African clawed frog).
Q5SV91_MOUSE Dual specificity phosphatase 14 (16 days neonate cerebellum cDNA, RIKEN full-length enriched library, clone:9630043M20 product:dual specificity phosphatase 14, full insert sequence) (Blastocyst blastocyst cDNA, RIKEN full-length enriched library, clone:I1C0016E23 product:dual specificity phosphatase 14, full insert sequence) - Mus musculus (Mouse).
Q5XJN5_BRARE Zgc:101746 - Brachydanio rerio (Zebrafish) (Danio rerio).
Q6FI36_HUMAN DUSP14 protein - Homo sapiens (Human).
Q6GM01_XENLA MGC84083 protein - Xenopus laevis (African clawed frog).
Q7TPY1_MOUSE Dusp14 protein - Mus musculus (Mouse).
Q9D9D8_MOUSE Adult male testis cDNA, RIKEN full-length enriched library, clone:1700094E07 product:hypothetical Dual specificity protein phosphatase containing protein, full insert sequence (Dual specificity phosphatase 21) - Mus musculus (Mouse).
Q9Y346_HUMAN Hypothetical protein (BJ-HCC-26 tumor antigen) - Homo sapiens (Human).
Scan History
SPTR49_32f 2  100  NSINGLE    
Initial Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
QITSSLFLGRGSVASNR DUS14_HUMAN 29 29 -
QITSSLFLGRASVASNW DUS14_MOUSE 29 29 -
QITKSLYISNGVAANNK DUS18_PONPY 22 22 -
QITSSLYISSGVAANNR DUS18_BOVIN 22 22 -
QITKSLYISNGVAANNK DUS18_HUMAN 22 22 -
QITSSLFLGRASVASNW Q7TPY1_MOUSE 29 29 -
QITKSLFISNGAAANDK DUS18_RAT 22 22 -
QITKSLFISNGVAANNK DUS18_MOUSE 22 22 -
QITASLFISNSAVANDK Q9D9D8_MOUSE 23 23 -
QITRSLFLSNGVAANDK DUS21_HUMAN 24 24 -
QITRSLFLSNGVAANDK Q9Y346_HUMAN 24 24 -

Motif 2 width=15
Element Seqn Id St Int Rpt
LQARGITCIVNATIE DUS14_HUMAN 48 2 -
LQARGITCVINATIE DUS14_MOUSE 48 2 -
LSSNQITMVINVSVE DUS18_PONPY 41 2 -
LSSNRISTVINVSVE DUS18_BOVIN 41 2 -
LSSNQITMVINVSVE DUS18_HUMAN 41 2 -
LQARGITCDINATIE Q7TPY1_MOUSE 48 2 -
LSSNQITTVINVSVE DUS18_RAT 41 2 -
LSSNQITTVINVSVE DUS18_MOUSE 41 2 -
LSNNHITTIINVSAE Q9D9D8_MOUSE 42 2 -
LSSNRITAIVNASVE DUS21_HUMAN 43 2 -
LSSNRITAIVNASVG Q9Y346_HUMAN 43 2 -

Motif 3 width=10
Element Seqn Id St Int Rpt
YVKVPLADMP DUS14_HUMAN 73 10 -
YVKVPLADIP DUS14_MOUSE 73 10 -
YLQVPVADAP DUS18_PONPY 66 10 -
YVQVPVADTP DUS18_BOVIN 66 10 -
YMQVPVADSP DUS18_HUMAN 66 10 -
YVKVPLADIP Q7TPY1_MOUSE 73 10 -
YVQVPVVDAP DUS18_RAT 66 10 -
YVQVPVVDAP DUS18_MOUSE 66 10 -
YVQVPVSDAP Q9D9D8_MOUSE 67 10 -
YIKVPVTDAR DUS21_HUMAN 68 10 -
YIKVPVTDAR Q9Y346_HUMAN 68 10 -

Motif 4 width=15
Element Seqn Id St Int Rpt
YFDTVADKIHSVSRK DUS14_HUMAN 89 6 -
YFDTVADKIHSVSKK DUS14_MOUSE 89 6 -
FFDPVADHIHSVEMK DUS18_PONPY 82 6 -
FFDPIADHIHSVEMK DUS18_BOVIN 82 6 -
FFDPIADHIHSVEMK DUS18_HUMAN 82 6 -
YFDTVADKIHSVSKK Q7TPY1_MOUSE 89 6 -
FFDPIADHIHSVEMK DUS18_RAT 82 6 -
FFDSVADRIHSVEMQ DUS18_MOUSE 82 6 -
FFDPIADHIHGVEMR Q9D9D8_MOUSE 83 6 -
FFDPIADLIHTIDMR DUS21_HUMAN 84 6 -
FFDPIADLIHTIDMR Q9Y346_HUMAN 84 6 -
Final Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
QITSSLFLGRASVASNR Q3TLZ7_MOUSE 29 29 -
QITSSLFLGRGSVASNR DUS14_HUMAN 29 29 -
QITSSLFLGRGSVASNR Q6FI36_HUMAN 29 29 -
QITSSLFLGRGSVASNR Q25DK0_MACFA 29 29 -
QITSSLFLGRASVASNW DUS14_MOUSE 29 29 -
QITSSLFLGRASVASNW Q5SV91_MOUSE 29 29 -
QITKSLYISNGVAANNK Q4R3T3_MACFA 22 22 -
QITKSLYISNGVAANNK DUS18_PONPY 22 22 -
QITSSLYISRGVAANNR Q32KN1_BOVIN 22 22 -
QITSSLYISSGVAANNR DUS18_BOVIN 22 22 -
QITKSLYISNGVAANNK DUS18_HUMAN 22 22 -
QITSSLFLGRASVASNW Q7TPY1_MOUSE 29 29 -
QITKSLFISNGAAANDK DUS18_RAT 22 22 -
QITKSLFISNGVAANNK DUS18_MOUSE 22 22 -
QITPSLFLGRGNVASNR Q5XJN5_BRARE 52 52 -
QITASLFISNSAVANDK Q9D9D8_MOUSE 23 23 -
QITRSLFLSNGVAANDK DUS21_HUMAN 24 24 -
QITRSLFLSNGVAANDK Q9Y346_HUMAN 24 24 -
QISPCLYLSSGNAAGSR Q6GM01_XENLA 39 39 -
QISPCLYLSSGNAAGSR Q5PQ70_XENLA 35 35 -
RISEGLYLASAKAASNR Q3KQ00_XENLA 25 25 -

Motif 2 width=15
Element Seqn Id St Int Rpt
LQARGITCVINATIE Q3TLZ7_MOUSE 48 2 -
LQARGITCIVNATIE DUS14_HUMAN 48 2 -
LQARGITCIVNATIE Q6FI36_HUMAN 48 2 -
LQARGITCIVNATIE Q25DK0_MACFA 48 2 -
LQARGITCVINATIE DUS14_MOUSE 48 2 -
LQARGITCVINATIE Q5SV91_MOUSE 48 2 -
LSSNQITMVINVSVE Q4R3T3_MACFA 41 2 -
LSSNQITMVINVSVE DUS18_PONPY 41 2 -
LSSNRISTVINVSVE Q32KN1_BOVIN 41 2 -
LSSNRISTVINVSVE DUS18_BOVIN 41 2 -
LSSNQITMVINVSVE DUS18_HUMAN 41 2 -
LQARGITCDINATIE Q7TPY1_MOUSE 48 2 -
LSSNQITTVINVSVE DUS18_RAT 41 2 -
LSSNQITTVINVSVE DUS18_MOUSE 41 2 -
LLSKGITCVVNATIE Q5XJN5_BRARE 71 2 -
LSNNHITTIINVSAE Q9D9D8_MOUSE 42 2 -
LSSNRITAIVNASVE DUS21_HUMAN 43 2 -
LSSNRITAIVNASVG Q9Y346_HUMAN 43 2 -
LYSRNVTCIVNATLE Q6GM01_XENLA 58 2 -
VYSRNVTCIVNATLE Q5PQ70_XENLA 54 2 -
LATHRITCVINVSQE Q3KQ00_XENLA 44 2 -

Motif 3 width=10
Element Seqn Id St Int Rpt
YVKVPLADIP Q3TLZ7_MOUSE 73 10 -
YVKVPLADMP DUS14_HUMAN 73 10 -
YVKVPLADMP Q6FI36_HUMAN 73 10 -
YVKVPLADMP Q25DK0_MACFA 73 10 -
YVKVPLADIP DUS14_MOUSE 73 10 -
YVKVPLADIP Q5SV91_MOUSE 73 10 -
YMQVPVADAP Q4R3T3_MACFA 66 10 -
YLQVPVADAP DUS18_PONPY 66 10 -
YVQVPVADTP Q32KN1_BOVIN 66 10 -
YVQVPVADTP DUS18_BOVIN 66 10 -
YMQVPVADSP DUS18_HUMAN 66 10 -
YVKVPLADIP Q7TPY1_MOUSE 73 10 -
YVQVPVVDAP DUS18_RAT 66 10 -
YVQVPVVDAP DUS18_MOUSE 66 10 -
YVKVPLADMP Q5XJN5_BRARE 96 10 -
YVQVPVSDAP Q9D9D8_MOUSE 67 10 -
YIKVPVTDAR DUS21_HUMAN 68 10 -
YIKVPVTDAR Q9Y346_HUMAN 68 10 -
YMKVPVPDLP Q6GM01_XENLA 83 10 -
YMKVPVPDLP Q5PQ70_XENLA 79 10 -
YVNIPVSDTP Q3KQ00_XENLA 69 10 -

Motif 4 width=15
Element Seqn Id St Int Rpt
YFDTVADKIHSVSKK Q3TLZ7_MOUSE 89 6 -
YFDTVADKIHSVSRK DUS14_HUMAN 89 6 -
YFDTVADKIHSVSRK Q6FI36_HUMAN 89 6 -
YFDTVADKIHSVSRK Q25DK0_MACFA 89 6 -
YFDTVADKIHSVSKK DUS14_MOUSE 89 6 -
YFDTVADKIHSVSKK Q5SV91_MOUSE 89 6 -
FFDPIADHIHSVEMK Q4R3T3_MACFA 82 6 -
FFDPVADHIHSVEMK DUS18_PONPY 82 6 -
FFDPIADHIHSVEMK Q32KN1_BOVIN 82 6 -
FFDPIADHIHSVEMK DUS18_BOVIN 82 6 -
FFDPIADHIHSVEMK DUS18_HUMAN 82 6 -
YFDTVADKIHSVSKK Q7TPY1_MOUSE 89 6 -
FFDPIADHIHSVEMK DUS18_RAT 82 6 -
FFDSVADRIHSVEMQ DUS18_MOUSE 82 6 -
YFDSVADKIHSVGRK Q5XJN5_BRARE 112 6 -
FFDPIADHIHGVEMR Q9D9D8_MOUSE 83 6 -
FFDPIADLIHTIDMR DUS21_HUMAN 84 6 -
FFDPIADLIHTIDMR Q9Y346_HUMAN 84 6 -
YFDSVADRIHQNGKR Q6GM01_XENLA 99 6 -
YFDSVADRIHQNGKR Q5PQ70_XENLA 95 6 -
YFEDIADKIHTVKAG Q3KQ00_XENLA 85 6 -