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PR01895

Identifier
WNT16PROTEIN  [View Relations]  [View Alignment]  
Accession
PR01895
No. of Motifs
6
Creation Date
27-MAY-2003
Title
Wnt-16 protein signature
Database References
PRINTS; PR01349 WNTPROTEIN
MIM; 606267
Literature References
1. WODARZ, A. AND NUSSE, R.
Mechanisms of Wnt signal transduction.
ANNU.REV.CELL DEV.BIOL. 14 59-88 (1998).
 
2. BEJSOVEC, A.
Signal transduction: Wnt signalling shows its versatility.
CURR.BIOL. 9 R684-R687 (1999).
 
3. DE FERRARI, G.V. AND INESTROSA, N.C.
Wnt signaling function in Alzheimer's disease.
BRAIN RES.REV. 33 1-12 (2000).
 
4. SEMENOV, M.V. AND SNYDER, M.
Human dishevelled genes constitute a DHR-containing multigene family.
GENOMICS 42 302-310 (1997).
 
5. PEIFER, M. AND POLAKIS, P.
Wnt signalling in oncogenesis and embryogenesis - a look outside the
nucleus.
SCIENCE 287 1606-1609 (2000).
 
6. MOON, R.T.
An introduction to non-canonical Wnt and Frizzled signaling.
SEMIN.CELL DEV.BIOL. 13 215 (2002).
 
7. MCWHIRTER, J.R., NEUTEBOOM, S.T., WANCEWICZ, E.V., MONIA, B.P., 
DOWNING, J.R. AND MURRE, C.
Oncogenic homeodomain transcription factor E2A-Pbx1 activates a novel
WNT gene in pre-B acute lymphoblastoid leukemia.
PROC.NATL.ACAD.SCI.U.S.A. 96 11464-11469 (1999).

Documentation
Wnt proteins constitute a large family of secreted molecules that are
involved in intercellular signalling during development. The name derives
from the first 2 members of the family to be discovered: int-1 (mouse) and
wingless (Drosophila) [1]. It is now recognised that Wnt signalling controls
many cell fate decisions in a variety of different organisms, including
mammals [2]. Wnt signalling has been implicated in tumorigenesis, early
mesodermal patterning of the embryo, morphogenesis of the brain and kidney,
regulation of mammary gland proliferation, and Alzheimer's disease [3,4].
 
Wnt-mediated signalling is believed to proceed initially through binding to
cell surface receptors of the frizzled family; the signal is subsequently
transduced through several cytoplasmic components to B-catenin, which enters
the nucleus and activates the transcription of several genes important in
development [5]. More recently, however, several non-canonical Wnt
signalling pathways have been elucidated that act independently of
B-catenin [6]. Members of the Wnt gene family are defined by their sequence
similarity to mouse Wnt-1 and Wingless in Drosophila. They encode proteins
of ~350-400 residues in length, with orthologues identified in several,
mostly vertebrate, species. Very little is known about the structure of 
Wnts as they are notoriously insoluble; in terms of primary structure, the
family is characterised by a signal sequence and an almost invariant pattern
of 23-24 conserved cysteines [1]. Fifteen major Wnt gene families have been 
identified in vertebrates, with multiple subtypes within some classes.
 
Expression of Wnt-16 is activated by E2A-Pbx1, a fusion protein resulting 
from a t(1;19) translocation that occurs in a large proportion of pediatric 
pre-B acute lymphoblastoid leukaemias (pre-B ALL) [7]. The Wnt-16 
transcript, normally absent in bone marrow, was found to be highly expressed 
in the bone marrow of pre-B ALL patients.
 
WNT16PROTEIN is a 6-element fingerprint that provides a signature for the 
Wnt-16 proteins. The fingerprint was derived from an initial alignment of 2 
sequences: the motifs were drawn from conserved regions spanning the full
alignment length, focusing on those sections that characterise Wnt-16 
proteins but distinguish them from other Wnt subtypes - motif 1 lies at the 
N-terminus, within the putative signal peptide; motif 2 resides within the 
N-terminal portion, following the signal peptide; motifs 3-5 are located 
between highly conserved cysteines; and motif 6 includes conserved Cys16. A 
single iteration on SPTR40_22f was required to reach convergence, no further
sequences being identified beyond the starting set.
Summary Information
2 codes involving  6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
6222222
5000000
4000000
3000000
2000000
123456
True Positives
WN16_HUMAN    WN16_MOUSE    
Sequence Titles
WN16_HUMAN  WNT-16 protein precursor - Homo sapiens (Human). 
WN16_MOUSE WNT-16 protein precursor - Mus musculus (Mouse).
Scan History
SPTR40_22f 1  100  NSINGLE    
Initial Motifs
Motif 1  width=21
Element Seqn Id St Int Rpt
AALLGLARLCALWAALLVLFP WN16_HUMAN 4 4 -
AALLALPSLCALWAAVLSLLP WN16_MOUSE 4 4 -

Motif 2 width=12
Element Seqn Id St Int Rpt
IASFGVPEKLGC WN16_HUMAN 36 11 -
IASFGVPEKLGC WN16_MOUSE 36 11 -

Motif 3 width=14
Element Seqn Id St Int Rpt
AAATTAPMGASPLF WN16_HUMAN 96 48 -
ATTTSTQLATAPLF WN16_MOUSE 95 47 -

Motif 4 width=17
Element Seqn Id St Int Rpt
LDFPIGNTTGKENKVLL WN16_HUMAN 183 73 -
LDLPIRNTTGKESRVLL WN16_MOUSE 182 73 -

Motif 5 width=14
Element Seqn Id St Int Rpt
REKDQRKIPIHKDD WN16_HUMAN 270 70 -
REKDQRQTPILKDD WN16_MOUSE 269 70 -

Motif 6 width=11
Element Seqn Id St Int Rpt
LLYVNKSPNYC WN16_HUMAN 284 0 -
LLYVHKSPNYC WN16_MOUSE 283 0 -
Final Motifs
Motif 1  width=21
Element Seqn Id St Int Rpt
AALLGLARLCALWAALLVLFP WN16_HUMAN 4 4 -
AALLALPSLCALWAAVLSLLP WN16_MOUSE 4 4 -

Motif 2 width=12
Element Seqn Id St Int Rpt
IASFGVPEKLGC WN16_HUMAN 36 11 -
IASFGVPEKLGC WN16_MOUSE 36 11 -

Motif 3 width=14
Element Seqn Id St Int Rpt
AAATTAPMGASPLF WN16_HUMAN 96 48 -
ATTTSTQLATAPLF WN16_MOUSE 95 47 -

Motif 4 width=17
Element Seqn Id St Int Rpt
LDFPIGNTTGKENKVLL WN16_HUMAN 183 73 -
LDLPIRNTTGKESRVLL WN16_MOUSE 182 73 -

Motif 5 width=14
Element Seqn Id St Int Rpt
REKDQRKIPIHKDD WN16_HUMAN 270 70 -
REKDQRQTPILKDD WN16_MOUSE 269 70 -

Motif 6 width=11
Element Seqn Id St Int Rpt
LLYVNKSPNYC WN16_HUMAN 284 0 -
LLYVHKSPNYC WN16_MOUSE 283 0 -