SPRINT Home UMBER Home Contents Standard Search Advanced Search Relation Search

==SPRINT==> PRINTS View



  selected as


PR01892

Identifier
WNT8PROTEIN  [View Relations]  [View Alignment]  
Accession
PR01892
No. of Motifs
4
Creation Date
13-MAY-2003
Title
Wnt-8 protein signature
Database References
PRINTS; PR01349 WNTPROTEIN
MIM; 606360; 601396
Literature References
1. WODARZ, A. AND NUSSE, R.
Mechanisms of Wnt signal transduction.
ANNU.REV.CELL DEV.BIOL. 14 59-88 (1998).
 
2. BEJSOVEC, A.
Signal transduction: Wnt signalling shows its versatility.
CURR.BIOL. 9 R684-R687 (1999).
 
3. DE FERRARI, G.V. AND INESTROSA, N.C.
Wnt signaling function in Alzheimer's disease.
BRAIN RES.REV. 33 1-12 (2000).
 
4. SEMENOV, M.V. AND SNYDER, M.
Human dishevelled genes constitute a DHR-containing multigene family.
GENOMICS 42 302-310 (1997).
 
5. PEIFER, M. AND POLAKIS, P.
Wnt signalling in oncogenesis and embryogenesis - a look outside the 
nucleus.
SCIENCE 287 1606-1609 (2000).
 
6. MOON, R.T.
An introduction to non-canonical Wnt and Frizzled signaling.
SEMIN.CELL DEV.BIOL. 13 215 (2002).
 
7. CHRISTIAN, J.L., MCMAHON, J.A., MCMAHON, A.P. AND MOON, R.T.
Xwnt-8, a Xenopus Wnt-1/int-1-related gene responsive to mesoderm-inducing 
growth factors, may play a role in ventral mesodermal patterning during 
embryogenesis.
DEVELOPMENT 111 1045-1055 (1991).

Documentation
Wnt proteins constitute a large family of secreted molecules that are
involved in intercellular signalling during development. The name derives
from the first 2 members of the family to be discovered: int-1 (mouse) and
wingless (Drosophila) [1]. It is now recognised that Wnt signalling controls
many cell fate decisions in a variety of different organisms, including
mammals [2]. Wnt signalling has been implicated in tumorigenesis, early
mesodermal patterning of the embryo, morphogenesis of the brain and kidney,
regulation of mammary gland proliferation, and Alzheimer's disease [3,4].
 
Wnt-mediated signalling is believed to proceed initially through binding to
cell surface receptors of the frizzled family; the signal is subsequently
transduced through several cytoplasmic components to B-catenin, which enters
the nucleus and activates the transcription of several genes important in
development [5]. More recently, however, several non-canonical Wnt
signalling pathways have been elucidated that act independently of
B-catenin [6]. Members of the Wnt gene family are defined by their sequence
similarity to mouse Wnt-1 and Wingless in Drosophila. They encode proteins
of ~350-400 residues in length, with orthologues identified in several,
mostly vertebrate, species. Very little is known about the structure of 
Wnts as they are notoriously insoluble; in terms of primary structure, the
family is characterised by a signal sequence and an almost invariant pattern
of 23-24 conserved cysteines [1]. Fifteen major Wnt gene families have been 
identified in vertebrates, with multiple subtypes within some classes.
 
Wnt-8 cDNA was first isolated from Xenopus, with orthologues later being 
identified in several other species including human, mouse and zebrafish. 
Alternative exon splicing gives rise to several Wnt-8 isoforms [7].
 
WNT8PROTEIN is a 4-element fingerprint that provides a signature for Wnt-8
proteins. The fingerprint was derived from an initial alignment of 10
sequences: the motifs were drawn from conserved regions spanning the full
alignment length, focusing on those sections that characterise Wnt-8
proteins but distinguish them from other Wnt subtypes - motif 1 lies at the
N-terminus, following the putative signal peptide; motifs 2 and 3 reside
between conserved cysteines; and motif 4 includes conserved cysteines
13-15. Two iterations on SPTR40_22f were required to reach convergence,
at which point a true set comprising 12 sequences was identified.
Summary Information
12 codes involving  4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
412121212
30000
20000
1234
True Positives
Q90YJ8        Q90YL8        Q90ZA3        WN8A_HUMAN    
WN8A_MOUSE WN8B_BRARE WN8B_HUMAN WN8B_MOUSE
WN8B_XENLA WN8C_CHICK WNT8_BRARE WNT8_XENLA
Sequence Titles
Q90YJ8      Wnt8-like protein 2 - Brachydanio rerio (Zebrafish) (Danio rerio). 
Q90YL8 Wnt8-like protein 2 - Brachydanio rerio (Zebrafish) (Danio rerio).
Q90ZA3 Secreted factor Axwnt-8 - Ambystoma mexicanum (Axolotl).
WN8A_HUMAN
WN8A_MOUSE
WN8B_BRARE Wnt-8b protein precursor - Brachydanio rerio (Zebrafish) (Danio rerio).
WN8B_HUMAN WNT-8B protein precursor - Homo sapiens (Human).
WN8B_MOUSE WNT-8B protein precursor - Mus musculus (Mouse).
WN8B_XENLA WNT-8B protein precursor (XWNT-8B) - Xenopus laevis (African clawed frog).
WN8C_CHICK WNT-8C protein precursor (CWNT-8) - Gallus gallus (Chicken).
WNT8_BRARE Wnt-8 protein precursor - Brachydanio rerio (Zebrafish) (Danio rerio).
WNT8_XENLA WNT-8 protein precursor (XWNT-8) - Xenopus laevis (African clawed frog).
Scan History
SPTR40_22f 2  100  NSINGLE    
Initial Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
LMTGPKAYLAYTS WNT8_BRARE 30 30 -
LMTGPKAYLIYSS WN8B_XENLA 29 29 -
LMTGPKAYLTYSS WN8C_CHICK 30 30 -
LMTGPKAYLVYSS WN8B_MOUSE 28 28 -
LMTGPKAYLTYSA WNT8_XENLA 30 30 -
LMTGPKAYLIYSS WN8B_HUMAN 29 29 -
LMTGPKAYLIYSS WN8B_BRARE 30 30 -
LITGPKAYLTYTT WN8A_HUMAN 29 29 -
LITGPKAYLTYTT WN8A_HUMAN 29 29 -
LITGPKAYLTYTA WN8A_MOUSE 29 29 -

Motif 2 width=10
Element Seqn Id St Int Rpt
KMGGRGWVWG WNT8_BRARE 122 79 -
QLGGQGWLWG WN8B_XENLA 194 152 -
RVGGRGWVWG WN8C_CHICK 122 79 -
QLGGQGWLWG WN8B_MOUSE 120 79 -
RIGGRGWVWG WNT8_XENLA 122 79 -
QLGGQGWLWG WN8B_HUMAN 121 79 -
QRGGQGWLWG WN8B_BRARE 122 79 -
KTGGHGWIWG WN8A_HUMAN 121 79 -
KTGGHGWIWG WN8A_HUMAN 121 79 -
KTGGHGWIWG WN8A_MOUSE 121 79 -

Motif 3 width=15
Element Seqn Id St Int Rpt
AGNSADNRGAIADTF WNT8_BRARE 228 96 -
AGNSAASRGAIAETF WN8B_XENLA 297 93 -
AGNSADSRGATAETF WN8C_CHICK 228 96 -
AGNSAAGRGAIADTF WN8B_MOUSE 223 93 -
SGNSADNRGAIADAF WNT8_XENLA 228 96 -
AGNSAAARGAIADTF WN8B_HUMAN 224 93 -
AGNSAASRGAIAETF WN8B_BRARE 225 93 -
AGNSAEGHWVPAEAF WN8A_HUMAN 227 96 -
AGNSAEGHWVPAEAF WN8A_HUMAN 227 96 -
AGNRAEGRWALTEAF WN8A_MOUSE 227 96 -

Motif 4 width=15
Element Seqn Id St Int Rpt
SQWERRSCRRLCHEC WNT8_BRARE 284 41 -
SKWEKRSCRRLCGDC WN8B_XENLA 353 41 -
SQWERRSCRRLSTEC WN8C_CHICK 284 41 -
GRWERRSCRRLCGDC WN8B_MOUSE 279 41 -
SQWERRSCKRLCTDC WNT8_XENLA 284 41 -
GRWELRSCRRLCGDC WN8B_HUMAN 280 41 -
SKWEKRTCKRLCGDC WN8B_BRARE 281 41 -
SRWERRSCGRLCTEC WN8A_HUMAN 283 41 -
SRWERRSCGRLCTEC WN8A_HUMAN 283 41 -
SRREQRSCGRLCTEC WN8A_MOUSE 283 41 -
Final Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
LMTGPKAYLTYST Q90ZA3 30 30 -
LMTGPKAYLAYTS WNT8_BRARE 30 30 -
LMTGPKAYLIYSS WN8B_XENLA 29 29 -
LMTGPKAYLTYSS WN8C_CHICK 30 30 -
LMTGPKAYLVYSS WN8B_MOUSE 28 28 -
LMTGPKAYLTYSA WNT8_XENLA 30 30 -
LMTGPKAYLIYSS WN8B_HUMAN 29 29 -
LITGPKAYLTYAN Q90YJ8 29 29 -
LITGPKAYLTYAN Q90YL8 29 29 -
LMTGPKAYLIYSS WN8B_BRARE 30 30 -
LITGPKAYLTYTT WN8A_HUMAN 29 29 -
LITGPKAYLTYTT WN8A_HUMAN 29 29 -
LITGPKAYLTYTA WN8A_MOUSE 29 29 -

Motif 2 width=10
Element Seqn Id St Int Rpt
RMGGRGWVWG Q90ZA3 122 79 -
KMGGRGWVWG WNT8_BRARE 122 79 -
QLGGQGWLWG WN8B_XENLA 194 152 -
RVGGRGWVWG WN8C_CHICK 122 79 -
QLGGQGWLWG WN8B_MOUSE 120 79 -
RIGGRGWVWG WNT8_XENLA 122 79 -
QLGGQGWLWG WN8B_HUMAN 121 79 -
RLGGRGWLWG Q90YJ8 121 79 -
RLGGRGWLWG Q90YL8 121 79 -
QRGGQGWLWG WN8B_BRARE 122 79 -
KTGGHGWIWG WN8A_HUMAN 121 79 -
KTGGHGWIWG WN8A_HUMAN 121 79 -
KTGGHGWIWG WN8A_MOUSE 121 79 -

Motif 3 width=15
Element Seqn Id St Int Rpt
AGNSADNRGAIAEAF Q90ZA3 228 96 -
AGNSADNRGAIADTF WNT8_BRARE 228 96 -
AGNSAASRGAIAETF WN8B_XENLA 297 93 -
AGNSADSRGATAETF WN8C_CHICK 228 96 -
AGNSAAGRGAIADTF WN8B_MOUSE 223 93 -
SGNSADNRGAIADAF WNT8_XENLA 228 96 -
AGNSAAARGAIADTF WN8B_HUMAN 224 93 -
AGNSADNRVTMTDAF Q90YJ8 227 96 -
AGNSADNRVTMTDAF Q90YL8 227 96 -
AGNSAASRGAIAETF WN8B_BRARE 225 93 -
AGNSAEGHWVPAEAF WN8A_HUMAN 227 96 -
AGNSAEGHWVPAEAF WN8A_HUMAN 227 96 -
AGNRAEGRWALTEAF WN8A_MOUSE 227 96 -

Motif 4 width=15
Element Seqn Id St Int Rpt
SQWEKRSCRRLCTEC Q90ZA3 284 41 -
SQWERRSCRRLCHEC WNT8_BRARE 284 41 -
SKWEKRSCRRLCGDC WN8B_XENLA 353 41 -
SQWERRSCRRLSTEC WN8C_CHICK 284 41 -
GRWERRSCRRLCGDC WN8B_MOUSE 279 41 -
SQWERRSCKRLCTDC WNT8_XENLA 284 41 -
GRWELRSCRRLCGDC WN8B_HUMAN 280 41 -
SQWERRSCRRLCHEC Q90YJ8 283 41 -
SQWERRSCRRLCHEC Q90YL8 283 41 -
SKWEKRTCKRLCGDC WN8B_BRARE 281 41 -
SRWERRSCGRLCTEC WN8A_HUMAN 283 41 -
SRWERRSCGRLCTEC WN8A_HUMAN 283 41 -
SRREQRSCGRLCTEC WN8A_MOUSE 283 41 -