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PR01843

Identifier
WNT3PROTEIN  [View Relations]  [View Alignment]  
Accession
PR01843
No. of Motifs
3
Creation Date
06-JAN-2003
Title
Wnt-3 protein signature
Database References
PRINTS; PR01349 WNTPROTEIN
MIM; 165330
Literature References
1. WODARZ, A. AND NUSSE, R.
Mechanisms of Wnt signal transduction.
ANNU.REV.CELL DEV.BIOL. 14 59-88 (1998).
 
2. BEJSOVEC, A.
Signal transduction: Wnt signalling shows its versatility.
CURR.BIOL. 9 R684-R687 (1999).
 
3. DE FERRARI, G.V. AND INESTROSA, N.C.
Wnt signaling function in Alzheimer's disease.
BRAIN RES.BRAIN RES.REV. 33 1-12 (2000).
 
4. SEMENOV, M.V. AND SNYDER, M.
Human dishevelled genes constitute a DHR-containing multigene family.
GENOMICS 42 302-310 (1997).
 
5. PEIFER, M. AND POLAKIS, P.
Wnt signalling in oncogenesis and embryogenesis - a look outside the
nucleus.
SCIENCE 287 1606-1609 (2000).
 
6. MOON, R.T.
An introduction to non-canonical Wnt and Frizzled signaling.
SEMIN.CELL DEV.BIOL. 13 215 (2002).
 
7. ROELINK, H., WAGENAAR, E., SILVA, S.L.D. AND NUSSE, R.
Wnt-3, a gene activated by proviral insertion in mouse mammary tumors, 
is homologous to int-1/Wnt-1 and is normally expressed in mouse embryos and
adult brain.
PROC.NATL.ACAD.SCI.U.S.A. 87 4519-4523 (1990).
 
8. ROELINK, H., WANG, J., BLACK, D.M., SOLOMON, E. AND NUSSE, R.
Molecular cloning and chromosomal localization to 17q21 of the human WNT3
gene.
GENOMICS 17 790-792 (1993).

Documentation
Wnt proteins constitute a large family of secreted molecules that are
involved in intercellular signalling during development. The name derives
from the first 2 members of the family to be discovered: int-1 (mouse) and
wingless (Drosophila) [1]. It is now recognised that Wnt signalling controls
many cell fate decisions in a variety of different organisms, including
mammals [2]. Wnt signalling has been implicated in tumorigenesis, early
mesodermal patterning of the embryo, morphogenesis of the brain and kidneys,
regulation of mammary gland proliferation and Alzheimer's disease [3,4].
 
Wnt-mediated signalling is believed to proceed initially through binding to
cell surface receptors of the frizzled family; the signal is subsequently
transduced through several cytoplasmic components to B-catenin, which enters
the nucleus and activates the transcription of several genes important in
development [5]. More recently, however, several non-canonical Wnt
signalling pathways have been elucidated that act independently of
B-catenin [6]. Members of the Wnt gene family are defined by their sequence
similarity to mouse Wnt-1 and Wingless in Drosophila. They encode proteins
of ~350-400 residues in length, with orthologues identified in several,
mostly vertebrate, species. Very little is known about the structure of 
Wnts as they are notoriously insoluble; in terms of primary structure, the
family is characterised by a signal sequence and an almost invariant pattern
of 23-24 conserved cysteines [1]. Fifteen major Wnt gene families have been 
identified in vertebrates, with multiple subtypes within some classes.
 
The Wnt-3 gene was first identified in mouse in 1987, where it was found to
be expressed during embryogenesis and in adult brain. Insertion of proviral
DNA from mouse mammary tumour virus (MMTV) occurs at the mouse Wnt-3 locus,
leading to activation of the gene [7]. However, the human Wnt-3 gene was not
found to be amplified or rearranged in breast cancer tissues [8].
 
WNT3PROTEIN is a 3-element fingerprint that provides a signature for
Wnt-3 proteins. The fingerprint was derived from an initial alignment of 2
sequences: the motifs were drawn from conserved regions spanning the C-
terminal half of the alignment, focusing on those sections that characterise
Wnt-3 proteins but distinguish them from related Wnt subtypes - the motifs
reside between a number of highly conserved cysteines. Two iterations on
SPTR40_20f were required to reach convergence, at which point a true set 
comprising 6 sequences was identified. A single partial match was also 
found, Q8WS76, a putative WNT3 protein from Branchiostoma floridae (Florida 
lancelet) that fails to make a significant match with motif 2.
Summary Information
   6 codes involving  3 elements
1 codes involving 2 elements
Composite Feature Index
3666
2101
123
True Positives
Q969P2        Q9PWH1        WN3A_MOUSE    WN3A_XENLA    
WNT3_HUMAN WNT3_MOUSE
True Positive Partials
Codes involving 2 elements
Q8WS76
Sequence Titles
Q969P2      CDNA FLJ31716 FIS, CLONE NT2RI2006565, HIGHLY SIMILAR TO WNT-3A PROTEIN PRECURSOR (WNT3A) - Homo sapiens (Human). 
Q9PWH1 WNT-3A - Gallus gallus (Chicken).
WN3A_MOUSE WNT-3A protein precursor - Mus musculus (Mouse).
WN3A_XENLA WNT-3A protein precursor (XWNT-3A) - Xenopus laevis (African clawed frog).
WNT3_HUMAN WNT-3 proto-oncogene protein precursor - Homo sapiens (Human).
WNT3_MOUSE WNT-3 proto-oncogene protein precursor - Mus musculus (Mouse).

Q8WS76 SECRETED GLYCOPROTEIN WNT3 - Branchiostoma floridae (Florida lancelet) (Amphioxus).
Scan History
SPTR40_20f 2  300  NSINGLE    
Initial Motifs
Motif 1  width=10
Element Seqn Id St Int Rpt
SHHKGPPGEG WNT3_HUMAN 143 143 -
SHHKGPPGEG WNT3_MOUSE 143 143 -

Motif 2 width=13
Element Seqn Id St Int Rpt
ADARENRPDARSA WNT3_HUMAN 173 20 -
ADARENRPDARSA WNT3_MOUSE 173 20 -

Motif 3 width=12
Element Seqn Id St Int Rpt
GHNTRTEKRKEK WNT3_HUMAN 318 132 -
GHNTRTEKRKEK WNT3_MOUSE 318 132 -
Final Motifs
Motif 1  width=10
Element Seqn Id St Int Rpt
SHHKGPPGEG WNT3_HUMAN 143 143 -
SHHKGPPGEG WNT3_MOUSE 143 143 -
TRHKGSPGEG Q9PWH1 164 164 -
THHKGPPGEG WN3A_XENLA 140 140 -
SRLQGSPGEG WN3A_MOUSE 140 140 -
SRHQGSPGKG Q969P2 140 140 -

Motif 2 width=13
Element Seqn Id St Int Rpt
ADARENRPDARSA WNT3_HUMAN 173 20 -
ADARENRPDARSA WNT3_MOUSE 173 20 -
ADARENRPDARSA Q9PWH1 194 20 -
ADARENRPDARSA WN3A_XENLA 170 20 -
ADARENRPDARSA WN3A_MOUSE 170 20 -
ADARENRPDARSA Q969P2 170 20 -

Motif 3 width=12
Element Seqn Id St Int Rpt
GHNTRTEKRKEK WNT3_HUMAN 318 132 -
GHNTRTEKRKEK WNT3_MOUSE 318 132 -
GHNTRTEKRKEK Q9PWH1 339 132 -
GQNTRTEKRKEK WN3A_XENLA 315 132 -
GHNARTERRREK WN3A_MOUSE 315 132 -
GHNARAERRREK Q969P2 315 132 -