SPRINT Home UMBER Home Contents Standard Search Advanced Search Relation Search

==SPRINT==> PRINTS View



  selected as


PR01842

Identifier
WNT2PROTEIN  [View Relations]  [View Alignment]  
Accession
PR01842
No. of Motifs
3
Creation Date
07-JAN-2003
Title
Wnt-2 protein signature
Database References
PRINTS; PR01349 WNTPROTEIN
MIM; 147870
Literature References
1. WODARZ, A. AND NUSSE, R.
Mechanisms of Wnt signal transduction.
ANNU.REV.CELL DEV.BIOL. 14 59-88 (1998).
 
2. BEJSOVEC, A.
Signal transduction: Wnt signalling shows its versatility.
CURR.BIOL. 9 R684-R687 (1999).
 
3. DE FERRARI, G.V. AND INESTROSA, N.C.
Wnt signaling function in Alzheimer's disease.
BRAIN RES.BRAIN RES.REV. 33 1-12 (2000).
 
4. SEMENOV, M.V. AND SNYDER, M.
Human dishevelled genes constitute a DHR-containing multigene family.
GENOMICS 42 302-310 (1997).
 
5. PEIFER, M. AND POLAKIS, P.
Wnt signalling in oncogenesis and embryogenesis - a look outside the
nucleus.
SCIENCE 287 1606-1609 (2000).
 
6. MOON, R.T.
An introduction to non-canonical Wnt and Frizzled signaling.
SEMIN.CELL DEV.BIOL. 13 215 (2002).
 
WICKING, C., ESTIVILL, X., COURTNEY, M., BOUE, A. AND PEDERSEN, P.S.
Isolation of a human gene with protein sequence similarity to human and
murine int-1 and the Drosophila segment polarity mutant wingless.
EMBO J. 7 1743-1748 (1988).

Documentation
7. WAINWRIGHT, B.J., SCAMBLER, P.J., STANIER, P., WATSON, E.K., BELL, G.,
Wnt proteins constitute a large family of secreted molecules that are
involved in intercellular signalling during development. The name derives
from the first 2 members of the family to be discovered: int-1 (mouse) and
wingless (Drosophila) [1]. It is now recognised that Wnt signalling controls
many cell fate decisions in a variety of different organisms, including
mammals [2]. Wnt signalling has been implicated in tumorigenesis, early
mesodermal patterning of the embryo, morphogenesis of the brain and kidneys,
regulation of mammary gland proliferation and Alzheimer's disease [3,4].
 
Wnt-mediated signalling is believed to proceed initially through binding to
cell surface receptors of the frizzled family; the signal is subsequently
transduced through several cytoplasmic components to B-catenin, which enters
the nucleus and activates the transcription of several genes important in
development [5]. More recently, however, several non-canonical Wnt
signalling pathways have been elucidated that act independently of
B-catenin [6]. Members of the Wnt gene family are defined by their sequence
similarity to mouse Wnt-1 and Wingless in Drosophila. They encode proteins
of ~350-400 residues in length, with orthologues identified in several,
mostly vertebrate, species. Very little is known about the structure of 
Wnts as they are notoriously insoluble; in terms of primary structure, the
family is characterised by a signal sequence and an almost invariant pattern
of 23-24 conserved cysteines [1]. Fifteen major Wnt gene families have been 
identified in vertebrates, with multiple subtypes within some classes.
 
The Wnt-2 gene was isolated from a human lung cDNA library in 1988 [7]. The 
protein was initially named IRP for Int-1 Related Protein and later termed 
Wnt-2. Northern blot analysis from a range of embryonic and adult tissues 
revealed that the gene was expressed almost exclusively in placenta and lung.
Mutation of the mouse Wnt-2 gene leads to placental defects.
 
WNT2PROTEIN is a 3-element fingerprint that provides a signature for Wnt-2
proteins. The fingerprint was derived from an initial alignment of 3
sequences: the motifs were drawn from conserved regions spanning the N-
terminal half of the alignment, focusing on those sections that characterise
Wnt-2 proteins but distinguish them from related Wnt subtypes - motif 1 
includes the first conserved cysteine; and motif 3 lies in the central 
portion of the protein. Two iterations on SPTR40_20f were required to reach
convergence, at which point a true set comprising 9 sequences was identified.
Summary Information
9 codes involving  3 elements
0 codes involving 2 elements
Composite Feature Index
3999
2000
123
True Positives
Q8UW39        Q98SN7        Q9CZW3        WN13_HUMAN    
WN2B_MOUSE WN2B_XENLA WNT2_BRARE WNT2_HUMAN
WNT2_MOUSE
Sequence Titles
Q8UW39      WNT2 PROTEIN - Fugu rubripes (Japanese pufferfish) (Takifugu rubripes). 
Q98SN7 WG/INT-1 RELATED GENE PRODUCT WNT-2B - Gallus gallus (Chicken).
Q9CZW3 2610510E18RIK PROTEIN - Mus musculus (Mouse).
WN13_HUMAN WNT-13 PROTEIN PRECURSOR - HOMO SAPIENS (HUMAN).
WN2B_MOUSE WNT-2B protein precursor (WNT-13) - Mus musculus (Mouse).
WN2B_XENLA WNT-2B protein precursor (Xwnt-2b) - Xenopus laevis (African clawed frog).
WNT2_BRARE WNT-2 protein precursor - Brachydanio rerio (Zebrafish) (Zebra danio).
WNT2_HUMAN WNT-2 protein precursor (IRP protein) (Int-1 related protein) - Homo sapiens (Human).
WNT2_MOUSE WNT-2 protein precursor (IRP protein) (INT-1 related protein) - Mus musculus (Mouse).
Scan History
SPTR40_20f 2  300  NSINGLE    
Initial Motifs
Motif 1  width=12
Element Seqn Id St Int Rpt
SRVMCDNVPGLV WNT2_HUMAN 37 37 -
SRVMCDNVPGLV WNT2_MOUSE 37 37 -
SQVMCDNIPGLI WNT2_BRARE 35 35 -

Motif 2 width=11
Element Seqn Id St Int Rpt
TLDRDHSLFGR WNT2_HUMAN 89 40 -
TLDRDHSLFGR WNT2_MOUSE 89 40 -
TMAREHNLFGR WNT2_BRARE 87 40 -

Motif 3 width=10
Element Seqn Id St Int Rpt
RKGKDARALM WNT2_HUMAN 177 77 -
RKGKDARALM WNT2_MOUSE 177 77 -
RKERDARALM WNT2_BRARE 175 77 -
Final Motifs
Motif 1  width=12
Element Seqn Id St Int Rpt
SRVMCDNVPGLV Q9CZW3 37 37 -
SRVMCDNVPGLV WNT2_HUMAN 37 37 -
SRVMCDNVPGLV WNT2_MOUSE 37 37 -
ARVICDNIPGLV Q98SN7 62 62 -
ARVICDNIPGLV WN13_HUMAN 68 68 -
ARVICDNIPGLV WN2B_MOUSE 66 66 -
ARVICDNIPGLV WN2B_XENLA 28 28 -
SQVMCDNIPGLV Q8UW39 35 35 -
SQVMCDNIPGLI WNT2_BRARE 35 35 -

Motif 2 width=11
Element Seqn Id St Int Rpt
TLDRDHSLFGR Q9CZW3 89 40 -
TLDRDHSLFGR WNT2_HUMAN 89 40 -
TLDRDHSLFGR WNT2_MOUSE 89 40 -
TLDRDHTVFGR Q98SN7 114 40 -
TLDRDHTVFGR WN13_HUMAN 120 40 -
TLDRDHTVFGR WN2B_MOUSE 118 40 -
TLDRDHTVFGR WN2B_XENLA 80 40 -
TTARDHNLFGR Q8UW39 87 40 -
TMAREHNLFGR WNT2_BRARE 87 40 -

Motif 3 width=10
Element Seqn Id St Int Rpt
RKGKDARALM Q9CZW3 177 77 -
RKGKDARALM WNT2_HUMAN 177 77 -
RKGKDARALM WNT2_MOUSE 177 77 -
KKVKDARALM Q98SN7 202 77 -
KRLKDARALM WN13_HUMAN 208 77 -
KRLKDARALM WN2B_MOUSE 206 77 -
KRLKDARALM WN2B_XENLA 168 77 -
RKERDARALM Q8UW39 175 77 -
RKERDARALM WNT2_BRARE 175 77 -