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PR01764

Identifier
MAPKPHPHTASE  [View Relations]  [View Alignment]  
Accession
PR01764
No. of Motifs
5
Creation Date
18-JUL-2002
Title
MAP kinase phosphatase signature
Database References
PRINTS; PR00700 PRTYPHPHTASE; PR01908 ADSPHPHTASE; PR01911 PFDSPHPHTASE

PDB; 1HZM; 1VHR
SCOP; 1HZM; 1VHR
CATH; 1HZM; 1VHR
Literature References
1. SUN, H., CHARLES, C.H., LAU, L.F. AND TONKS, N.K. 
MKP-1 (3CH134), an immediate-early gene-product is a dual specificity
phosphatase that dephosphorylates MAP kinase in-vivo. 
CELL 75(3) 487-493 (1993).
 
2. MUDA, M., THEODOSIOU, A., RODRIGUES, N., BOSCHERT, U., CAMPS, M., 
GILLIERON, C., DAVIES, K., ASHWORTH, A. AND ARKINSTALL, S.
The dual specificity phosphatases M3/6 and MKP-3 are highly selective for 
inactivation of distinct mitogen activated protein kinases. 
J.BIOL.CHEM. 271(44) 27205-27208 (1996).
 
3. DIXON, J.E. AND SAPER, M.A.
Crystal structure of the dual specificity protein phosphatase VHR.
SCIENCE 272 1328-1331 (1996).

Documentation
The dual specificity phosphatases, also known as MAP Kinase Phosphatases 
(MKP), constitute a class of phosphatases that reverse the activation of MAP
(mitogen activated protein) kinases by dephosphorylating critical tyrosine 
and threonine residues [1]. This regulation is mediated via interaction of a 
Kinase Interaction Motif (KIM) with the common docking domain of the kinase 
- this motif is shared with a number of other protein families that interact 
with MAP kinases: these include kinases (MEKs), phosphatases (PTP-SL) and 
transcription factors (Elk1). MKPs also share an active site motif with the
protein tyrosine phosphatases (PTPs). Different MKPs exhibit specificity 
towards different members of the MAP kinase family [2].
 
The structure of the MPK catalytic domain is similar to that of the PTPs 
[3] - the fold exhibits a complex mixed alpha-/beta-architecture. The 
catalytic mechanism involves a general acid (Asp92 in 1VHR) and a catalytic 
cysteine (130 in 1VHR), which acts as a nucleophile.
 
MAPKPHPHTASE is a 5-element fingerprint that provides a signature for the 
MAP kinase phosphatases. The fingerprint was derived from an initial 
alignment of 12 sequences: the motifs were drawn from conserved regions 
spanning the full alignment length. Two iterations on SPTR40_20f were
required to reach convergence, at which point a true set comprising 30 
sequences was identified. Several partial matches were also found, most of
which appear to be N- or C-terminal fragments.
Summary Information
  30 codes involving  5 elements
0 codes involving 4 elements
5 codes involving 3 elements
2 codes involving 2 elements
Composite Feature Index
53030303030
400000
300555
211101
12345
True Positives
DUS1_HUMAN    DUS1_MOUSE    DUS1_RAT      DUS2_HUMAN    
DUS2_MOUSE DUS4_CHICK DUS4_HUMAN DUS4_RAT
DUS5_HUMAN DUS5_RAT DUS6_HUMAN DUS6_RAT
DUS8_HUMAN DUS8_MOUSE DUS9_HUMAN Q63683
Q64193 Q8UW48 Q90W58 Q91663
Q91790 Q920R2 Q96N49 Q99KC2
Q99MG5 Q99MG6 Q9AG15 Q9AG16
Q9CZY9 Q9ESS0
True Positive Partials
Codes involving 3 elements
Q8WXV0 Q95SV1 Q9H9Z5 Q9NSW1
Q9VVW4
Codes involving 2 elements
O44128 Q96QS2
Sequence Titles
DUS1_HUMAN  Dual specificity protein phosphatase 1 (EC 3.1.3.48) (EC 3.1.3.16) (MAP kinase phosphatase-1) (MKP-1) (Protein-tyrosine phosphatase CL100) (Dual specificity protein phosphatase hVH1) - Homo sapiens (Human). 
DUS1_MOUSE Dual specificity protein phosphatase 1 (EC 3.1.3.48) (EC 3.1.3.16) (MAP kinase phosphatase-1) (MKP-1) (Protein-tyrosine phosphatase 3CH134) (Protein-tyrosine phosphatase ERP) - Mus musculus (Mouse).
DUS1_RAT Dual specificity protein phosphatase 1 (EC 3.1.3.48) (EC 3.1.3.16) (MAP kinase phosphatase-1) (MKP-1) (Protein-tyrosine phosphatase CL100) - Rattus norvegicus (Rat).
DUS2_HUMAN Dual specificity protein phosphatase 2 (EC 3.1.3.48) (EC 3.1.3.16) (Dual specificity protein phosphatase PAC-1) - Homo sapiens (Human).
DUS2_MOUSE Dual specificity protein phosphatase 2 (EC 3.1.3.48) (EC 3.1.3.16) (Dual specificity protein phosphatase PAC-1) - Mus musculus (Mouse).
DUS4_CHICK Dual specificity protein phosphatase 4 (EC 3.1.3.48) (EC 3.1.3.16) (Mitogen-activated protein kinase phosphatase-2) (MAP kinase phosphatase-2) (MKP-2) - Gallus gallus (Chicken).
DUS4_HUMAN Dual specificity protein phosphatase 4 (EC 3.1.3.48) (EC 3.1.3.16) (Dual specificity protein phosphatase hVH2) - Homo sapiens (Human).
DUS4_RAT Dual specificity protein phosphatase 4 (EC 3.1.3.48) (EC 3.1.3.16) (Mitogen-activated protein kinase phosphatase-2) (MAP kinase phosphatase-2) (MKP-2) - Rattus norvegicus (Rat).
DUS5_HUMAN Dual specificity protein phosphatase 5 (EC 3.1.3.48) (EC 3.1.3.16) (Dual specificity protein phosphatase hVH3) - Homo sapiens (Human).
DUS5_RAT Dual specificity protein phosphatase 5 (EC 3.1.3.48) (EC 3.1.3.16) (MAP-kinase phosphatase CPG21) - Rattus norvegicus (Rat).
DUS6_HUMAN Dual specificity protein phosphatase 6 (EC 3.1.3.48) (EC 3.1.3.16) (Mitogen-activated protein kinase phosphatase 3) (MAP kinase phosphatase 3) (MKP-3) (Dual specificity protein phosphatase PYST1) - Homo sapiens (Human).
DUS6_RAT Dual specificity protein phosphatase 6 (EC 3.1.3.48) (EC 3.1.3.16) (Mitogen-activated protein kinase phosphatase 3) (MAP kinase phosphatase 3) (MKP-3) - Rattus norvegicus (Rat).
DUS8_HUMAN Dual specificity protein phosphatase 8 (EC 3.1.3.48) (EC 3.1.3.16) (Dual specificity protein phosphatase hVH-5) - Homo sapiens (Human).
DUS8_MOUSE Dual specificity protein phosphatase 8 (EC 3.1.3.48) (EC 3.1.3.16) (Neuronal tyrosine threonine phosphatase 1) - Mus musculus (Mouse).
DUS9_HUMAN Dual specificity protein phosphatase 9 (EC 3.1.3.48) (EC 3.1.3.16) (Mitogen-activated protein kinase phosphatase 4) (MAP kinase phosphatase 4) (MKP-4) - Homo sapiens (Human).
Q63683 PROTEIN TYROSINE PHOSPHATASE - Rattus norvegicus (Rat).
Q64193 3CH134/CL100 PTPASE - Rattus sp.
Q8UW48 DUAL SPECIFICITY PHOSPHATASE 6, ISOFORM A - Fugu rubripes (Japanese pufferfish) (Takifugu rubripes).
Q90W58 MAP KINASE PHOSPHATASE XCL100(BETA) PROTEIN - Xenopus laevis (African clawed frog).
Q91663 DUAL SPECIFICITY PROTEIN PHOSPHATASE ? (EC 3.1.3.48) (EC 3.1.3.16) (MAP KINASE PHOSPHATASE X17C) - Xenopus laevis (African clawed frog).
Q91790 DUAL SPECIFICITY PROTEIN PHOSPHATASE XCL100 (EC 3.1.3.48) (EC 3.1.3.16) (MAP KINASE PHOSPHATASE) - Xenopus laevis (African clawed frog).
Q920R2 MAP KINASE PHOSPHATASE-7 - Mus musculus (Mouse).
Q96N49 CDNA FLJ31411 FIS, CLONE NT2NE2000214, MODERATELY SIMILAR TO DUAL SPECIFICITY PROTEIN PHOSPHATASE 8 (EC 3.1.3.48) - Homo sapiens (Human).
Q99KC2 SIMILAR TO DUAL SPECIFICITY PHOSPHATASE 9 - Mus musculus (Mouse).
Q99MG5 MAP KINASE PHOSPHATASE-M A2 ISOFORM - Mus musculus (Mouse).
Q99MG6 MAP KINASE PHOSPHATASE-M A1 ISOFORM - Mus musculus (Mouse).
Q9AG15 MAP KINASE PHOSPHATASE-M B2 ISOFORM - Mus musculus (Mouse).
Q9AG16 MAP KINASE PHOSPHATASE-M B1 ISOFORM - Mus musculus (Mouse).
Q9CZY9 2610306G15RIK PROTEIN - Mus musculus (Mouse).
Q9ESS0 MAP KINASE PHOSPHATASE-5 - Mus musculus (Mouse).

Q8WXV0 PHOSPHATASE - Homo sapiens (Human).
Q95SV1 SD06439P - Drosophila melanogaster (Fruit fly).
Q9H9Z5 CDNA FLJ12451 FIS, CLONE NT2RM1000377, HIGHLY SIMILAR TO HOMO SAPIENS DUAL SPECIFICITY PHOSPHATASE MKP5 (MKP5) MRNA (DUAL SPECIFICITY PHOSPHATASE 10) - Homo sapiens (Human).
Q9NSW1 HYPOTHETICAL 33.0 KDA PROTEIN - Homo sapiens (Human).
Q9VVW4 CG14080 PROTEIN - Drosophila melanogaster (Fruit fly).

O44128 C05B10.1 PROTEIN - Caenorhabditis elegans.
Q96QS2 TRUNCATED MAPK PHOSPHATASE 7 - Homo sapiens (Human).
Scan History
SPTR40_20f 2  75   NSINGLE    
Initial Motifs
Motif 1  width=11
Element Seqn Id St Int Rpt
LLLDCRSFFAF DUS1_HUMAN 25 25 -
LLLDCRSFFAF DUS1_MOUSE 25 25 -
LLLDCRSFFAF DUS1_RAT 25 25 -
LLLDCRSFFAF Q64193 25 25 -
LILDCRSFFAF Q90W58 26 26 -
LLLDCRPFLAH DUS4_HUMAN 46 46 -
LLLDCRPFLAH DUS4_RAT 47 47 -
LLLDCRPFLAF DUS2_HUMAN 28 28 -
LLLDCRSRELY DUS9_HUMAN 23 23 -
LLMDCRPQELY DUS6_HUMAN 35 35 -
LLMDCRPQELY DUS6_RAT 35 35 -
VVLDCRPYLAF DUS5_RAT 24 24 -

Motif 2 width=13
Element Seqn Id St Int Rpt
TIVRRRAKGAMGL DUS1_HUMAN 50 14 -
TIVRRRAKGAMGL DUS1_MOUSE 50 14 -
TIVRRRAKGAMGL DUS1_RAT 50 14 -
TIVRRRAKGAMGL Q64193 50 14 -
TIVKRRAKGSMGL Q90W58 51 14 -
TIVRRRAKGSVSL DUS4_HUMAN 71 14 -
TIVRRRAKGSVSL DUS4_RAT 72 14 -
ALLRRRARGPPAA DUS2_HUMAN 53 14 -
LLLRRLRRGSLSV DUS9_HUMAN 49 15 -
IMLRRLQKGNLPV DUS6_HUMAN 61 15 -
IMLRRLQKGNLPV DUS6_RAT 61 15 -
VVLRRARGGAVSA DUS5_RAT 50 15 -

Motif 3 width=11
Element Seqn Id St Int Rpt
ITALINVSANC DUS1_HUMAN 200 137 -
ITALINVSANC DUS1_MOUSE 200 137 -
ITALINVSANC DUS1_RAT 200 137 -
ITALINVSANC Q64193 200 137 -
ITALINVSANC Q90W58 202 138 -
ITALLNVSSDC DUS4_HUMAN 222 138 -
ITALLNVSSDC DUS4_RAT 223 138 -
ITAVLNVSASC DUS2_HUMAN 199 133 -
IRYILNVTPNL DUS9_HUMAN 230 168 -
IKYILNVTPNL DUS6_HUMAN 233 159 -
IKYILNVTPNL DUS6_RAT 233 159 -
ITALLNVSRRT DUS5_RAT 205 142 -

Motif 4 width=10
Element Seqn Id St Int Rpt
YKSIPVEDNH DUS1_HUMAN 220 9 -
YKSIPVEDNH DUS1_MOUSE 220 9 -
YKSIPVEDNH DUS1_RAT 220 9 -
YKSIPVEDNH Q64193 220 9 -
YKSIPVEDSH Q90W58 222 9 -
YKCIPVEDNH DUS4_HUMAN 242 9 -
YKCIPVEDNH DUS4_RAT 243 9 -
YKSIPVEDNQ DUS2_HUMAN 219 9 -
YKQIPISDHW DUS9_HUMAN 252 11 -
YKQIPISDHW DUS6_HUMAN 255 11 -
YKQIPISDHW DUS6_RAT 255 11 -
YKWIPVEDSH DUS5_RAT 225 9 -

Motif 5 width=13
Element Seqn Id St Int Rpt
WFNEAIDFIDSIK DUS1_HUMAN 236 6 -
WFNEAIDFIDSIK DUS1_MOUSE 236 6 -
WFNEAIDFIDSIK DUS1_RAT 236 6 -
WFNEAIDFIDSIK Q64193 236 6 -
WFNEAIDFIDSIK Q90W58 238 6 -
WFMEAIEYIDAVK DUS4_HUMAN 258 6 -
WFMEAIEYIDAVK DUS4_RAT 259 6 -
WFQEAIGFIDWVK DUS2_HUMAN 235 6 -
FFPEAIEFIDEAL DUS9_HUMAN 268 6 -
FFPEAISFIDEAR DUS6_HUMAN 271 6 -
FFPEAISFIDEAR DUS6_RAT 271 6 -
HFQEAIDFIDCVR DUS5_RAT 241 6 -
Final Motifs
Motif 1  width=11
Element Seqn Id St Int Rpt
LLLDCRSFFAF DUS1_HUMAN 25 25 -
LLLDCRSFFAF DUS1_MOUSE 25 25 -
LLLDCRSFFAF DUS1_RAT 25 25 -
LLLDCRSFFAF Q63683 25 25 -
LLLDCRSFFAF Q64193 25 25 -
LILDCRSFFAF Q90W58 26 26 -
LILDCRSFFSF Q91790 26 26 -
LLLDCRPFLAH DUS4_CHICK 30 30 -
LLLDCRPFLAH DUS4_HUMAN 46 46 -
LLLDCRPFLAH DUS4_RAT 47 47 -
LLLDCRPFLAF DUS2_MOUSE 32 32 -
LLLDCRPFLAF DUS2_HUMAN 28 28 -
LLIDSRPFVEY Q920R2 27 27 -
LLIDSRPFVEY Q96N49 27 27 -
LLIDSRPFVEY Q99MG5 27 27 -
LLIDSRPFVEY Q99MG6 27 27 -
LLIDSRPFVEY Q9AG15 27 27 -
LLIDSRPFVEY Q9AG16 27 27 -
LLLDCRSRELY DUS9_HUMAN 23 23 -
LLMDCRPQELY DUS6_HUMAN 35 35 -
LLMDCRPQELY DUS6_RAT 35 35 -
LLLDCRSRELY Q99KC2 23 23 -
VVLDCRPYLAF DUS5_HUMAN 24 24 -
VVLDCRPYLAF DUS5_RAT 24 24 -
LVIDSRSFVEY DUS8_MOUSE 28 28 -
LLMDCRAHELY Q91663 34 34 -
LVIDSRSFVEY DUS8_HUMAN 28 28 -
LVMDCRAQELY Q8UW48 36 36 -
VIIDCRPFMEY Q9CZY9 174 174 -
VIIDCRPFMEY Q9ESS0 174 174 -

Motif 2 width=13
Element Seqn Id St Int Rpt
TIVRRRAKGAMGL DUS1_HUMAN 50 14 -
TIVRRRAKGAMGL DUS1_MOUSE 50 14 -
TIVRRRAKGAMGL DUS1_RAT 50 14 -
TIVRRRAKGAMGL Q63683 50 14 -
TIVRRRAKGAMGL Q64193 50 14 -
TIVKRRAKGSMGL Q90W58 51 14 -
TIVKRRAKGSMGL Q91790 51 14 -
TIVRRRAKGAVSL DUS4_CHICK 55 14 -
TIVRRRAKGSVSL DUS4_HUMAN 71 14 -
TIVRRRAKGSVSL DUS4_RAT 72 14 -
ALLRRRAPGTPAA DUS2_MOUSE 57 14 -
ALLRRRARGPPAA DUS2_HUMAN 53 14 -
KLMKRRLQQDKVL Q920R2 52 14 -
KLMKRRLQQDKVL Q96N49 52 14 -
KLMKRRLQQDRVL Q99MG5 52 14 -
KLMKRRLQQDRVL Q99MG6 52 14 -
KLMKRRLQQDRVL Q9AG15 52 14 -
KLMKRRLQQDRVL Q9AG16 52 14 -
LLLRRLRRGSLSV DUS9_HUMAN 49 15 -
IMLRRLQKGNLPV DUS6_HUMAN 61 15 -
IMLRRLQKGNLPV DUS6_RAT 61 15 -
LMLRRLRRGSMSV Q99KC2 49 15 -
VVLRRARGGAVSA DUS5_HUMAN 50 15 -
VVLRRARGGAVSA DUS5_RAT 50 15 -
KLVKRRLQQGKVT DUS8_MOUSE 53 14 -
IMLRRLKKGNLPI Q91663 60 15 -
KLVKRRLQQGKVT DUS8_HUMAN 53 14 -
LMLRRLKKGNLPV Q8UW48 62 15 -
KISRRRLQQGKIT Q9CZY9 200 15 -
KISRRRLQQGKIT Q9ESS0 200 15 -

Motif 3 width=11
Element Seqn Id St Int Rpt
ITALINVSANC DUS1_HUMAN 200 137 -
ITALINVSANC DUS1_MOUSE 200 137 -
ITALINVSANC DUS1_RAT 200 137 -
ITALINVSANC Q63683 200 137 -
ITALINVSANC Q64193 200 137 -
ITALINVSANC Q90W58 202 138 -
ITALINVSANC Q91790 202 138 -
ITALLNVSSDC DUS4_CHICK 203 135 -
ITALLNVSSDC DUS4_HUMAN 222 138 -
ITALLNVSSDC DUS4_RAT 223 138 -
ITAVLNVSASC DUS2_MOUSE 203 133 -
ITAVLNVSASC DUS2_HUMAN 199 133 -
IGYVLNASNTC Q920R2 185 120 -
IGYVLNASNTC Q96N49 185 120 -
IGYVLNASNTC Q99MG5 130 65 -
IGYVLNASNTC Q99MG6 185 120 -
IGYVLNASNTC Q9AG15 130 65 -
IGYVLNASNTC Q9AG16 185 120 -
IRYILNVTPNL DUS9_HUMAN 230 168 -
IKYILNVTPNL DUS6_HUMAN 233 159 -
IKYILNVTPNL DUS6_RAT 233 159 -
IRYILNVTPNL Q99KC2 282 220 -
ITALLNVSRRT DUS5_HUMAN 205 142 -
ITALLNVSRRT DUS5_RAT 205 142 -
ISYVLNASNSC DUS8_MOUSE 187 121 -
IKYILNVTPNL Q91663 230 157 -
ISYVLNASNSC DUS8_HUMAN 187 121 -
IKYILNVTPNL Q8UW48 233 158 -
IGYVINVTTHL Q9CZY9 349 136 -
IGYVINVTTHL Q9ESS0 349 136 -

Motif 4 width=10
Element Seqn Id St Int Rpt
YKSIPVEDNH DUS1_HUMAN 220 9 -
YKSIPVEDNH DUS1_MOUSE 220 9 -
YKSIPVEDNH DUS1_RAT 220 9 -
YKSIPVEDNH Q63683 220 9 -
YKSIPVEDNH Q64193 220 9 -
YKSIPVEDSH Q90W58 222 9 -
YKSIPVEDSH Q91790 222 9 -
YKCIPVEDNH DUS4_CHICK 223 9 -
YKCIPVEDNH DUS4_HUMAN 242 9 -
YKCIPVEDNH DUS4_RAT 243 9 -
YKSIPVEDNQ DUS2_MOUSE 223 9 -
YKSIPVEDNQ DUS2_HUMAN 219 9 -
FLRVPVNDSF Q920R2 206 10 -
FLRVPVNDSF Q96N49 206 10 -
FLRVPVNDSF Q99MG5 151 10 -
FLRVPVNDSF Q99MG6 206 10 -
FLRVPVNDSF Q9AG15 151 10 -
FLRVPVNDSF Q9AG16 206 10 -
YKQIPISDHW DUS9_HUMAN 252 11 -
YKQIPISDHW DUS6_HUMAN 255 11 -
YKQIPISDHW DUS6_RAT 255 11 -
YKQIPISDHW Q99KC2 304 11 -
YKWIPVEDSH DUS5_HUMAN 225 9 -
YKWIPVEDSH DUS5_RAT 225 9 -
FMRIPINDNY DUS8_MOUSE 208 10 -
YKQIPISDHW Q91663 252 11 -
FMRVPINDNY DUS8_HUMAN 208 10 -
YKQIPISDHW Q8UW48 255 11 -
YKRLPATDSN Q9CZY9 371 11 -
YKRLPATDSN Q9ESS0 371 11 -

Motif 5 width=13
Element Seqn Id St Int Rpt
WFNEAIDFIDSIK DUS1_HUMAN 236 6 -
WFNEAIDFIDSIK DUS1_MOUSE 236 6 -
WFNEAIDFIDSIK DUS1_RAT 236 6 -
WFNEAIDFIDSIK Q63683 236 6 -
WFNEAIDFIDSIK Q64193 236 6 -
WFNEAIDFIDSIK Q90W58 238 6 -
WFNEAIDFIDSVK Q91790 238 6 -
WFMEAIEYIDSVK DUS4_CHICK 239 6 -
WFMEAIEYIDAVK DUS4_HUMAN 258 6 -
WFMEAIEYIDAVK DUS4_RAT 259 6 -
WFQEAISFIDSVK DUS2_MOUSE 239 6 -
WFQEAIGFIDWVK DUS2_HUMAN 235 6 -
WLDKSVDFIEKAK Q920R2 222 6 -
WLDKSVDFIEKAK Q96N49 222 6 -
WLDKSVDFIEKAK Q99MG5 167 6 -
WLDKSVDFIEKAK Q99MG6 222 6 -
WLDKSVDFIEKAK Q9AG15 167 6 -
WLDKSVDFIEKAK Q9AG16 222 6 -
FFPEAIEFIDEAL DUS9_HUMAN 268 6 -
FFPEAISFIDEAR DUS6_HUMAN 271 6 -
FFPEAISFIDEAR DUS6_RAT 271 6 -
FFPEAIAFIDEAL Q99KC2 320 6 -
HFQEAIDFIDCVR DUS5_HUMAN 241 6 -
HFQEAIDFIDCVR DUS5_RAT 241 6 -
WLDKSIEFIDKAK DUS8_MOUSE 224 6 -
FFPEAISFIDEAR Q91663 268 6 -
WLDKSIEFIDKAK DUS8_HUMAN 224 6 -
FFPEAIGFIDEAR Q8UW48 271 6 -
YFEEAFEFIEEAH Q9CZY9 387 6 -
YFEEAFEFIEEAH Q9ESS0 387 6 -