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PR01409

Identifier
TCELLCD5  [View Relations]  [View Alignment]  
Accession
PR01409
No. of Motifs
10
Creation Date
30-MAR-2000
Title
T-cell surface glycoprotein CD5 signature
Database References
PRINTS; PR00258 SPERACTRCPTR
Literature References
1. DANGOTT, L.J., JORDAN, J.E., BELLET, R.A. AND GARBERS, D.L.
Cloning of the messenger RNA for the protein that cross-links to
egg peptide speract.
PROC.NATL.ACAD.SCI.U.S.A. 86 2128-2132 (1989).
 
2. FREEMAN, M., ASHKENAS, J., REES, D.J., KINGSLEY, D.M., COPELAND, N.G.,
JENKINS, N.A. AND KRIEGER, M.
An ancient, highly conserved family of cysteine-rich protein domains
revealed by cloning type-I and type-II murine macrophage scavenger
receptors.
PROC.NATL.ACAD.SCI.U.S.A. 87 8810-8814 (1990).
 
3. JONES, N.H., CLABBY, M.L., DIALYNAS, D.P., HUANG, H.J., HERZENBERG, L.A.
AND STROMINGER, J.L.
Isolation of complementary DNA clones encoding the human lymphocyte
glycoprotein T1/Leu-1.
NATURE 323 346-349 (1986). 
 
4.VAN DE VELDE, H., VON HOEGEN, I., LUO, W., PARNES, J.R. AND THIELEMANS, K. 
The B-cell surface protein CD72/Lyb-2 is the ligand for CD5.
NATURE 351 662-665 (1991). 

Documentation
The egg peptide speract receptor is a transmembrane glycoprotein of about
500 amino acids [1]. Topologically, it comprises a large extracellular
domain of about 450 residues, followed by a transmembrane domain and a
short cytoplasmic region of about 12 amino acids. The extracellular
domain contains 4 repeats of a well-conserved region, which spans 115
amino acids and contains 6 conserved cysteines. A similar domain is also
found towards the C-terminus of macrophage scavenger receptor type I [2],
a membrane glycoprotein implicated in the pathologic deposition of
cholesterol in arterial walls during artherogenesis, and in the CD5
glycoprotein, which acts as a receptor in regulating T-cell proliferation.
 
The T1/Leu-1/CD5 glycoprotein is expressed at the surface membrane of all
mature T cells [3,4]. It has been implicated both in the proliferative 
response of activated T cells and in T-cell helper function [3]. The
complete amino-acid sequence of the T1 precursor has been deduced from cDNA
clones. The protein contains a classical signal peptide; a 347-residue 
extracellular segment; a transmembrane region; and a 93-residue intra-
cellular segment [3]. The extracellular region contains several cysteine
residues and comprises 2 speract receptor domains separated by a proline/
threonine-rich region [3]. CD5 has been shown to function as a receptor,
delivering co-stimulatory signals to T-cells, interacting specifically with
the cell-surface protein CD72 (Lyb-2 in mice) exclusive to B-cells [4].   
 
TCELLCD5 is a 10-element fingerprint that provides a signature for the
T-cell surface glycoprotein CD5 precursor. The fingerprint was derived from 
an initial alignment of 4 sequences: the motifs were drawn from conserved
regions spanning virtually the full alignment length, focusing on those
sections that characterise the CD5 proteins but distuinguish them from the
speract receptor family - motifs 1-4 span the region between the 2 speract
receptor domains (motif 4 includes the N-terminal portion of the second 
domain); motif 5 resides within the second speract domain; motif 6 encodes
the transmembrane domain; and motifs 7-10 span the cytoplasmic domain. A
single iteration on SPTR37_10f was required to reach convergence, no further
sequences being identified beyond the starting set. A single partial match
was found, O73906, a chicken CD5 mRNA precursor that matches motifs 1, 4 
and 6-10.
Summary Information
   4 codes involving 10 elements
0 codes involving 9 elements
0 codes involving 8 elements
1 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
104444444444
90000000000
80000000000
71001011111
60000000000
50000000000
40000000000
30000000000
20000000000
12345678910
True Positives
CD5_BOVIN     CD5_HUMAN     CD5_MOUSE     CD5_RAT       
True Positive Partials
Codes involving 7 elements
O73906
Sequence Titles
CD5_BOVIN   T-CELL SURFACE GLYCOPROTEIN CD5 PRECURSOR (LYMPHOCYTE GLYCOPROTEIN T1/LEU-1) (LYMPHOCYTE ANTIGEN CD5) - BOS TAURUS (BOVINE). 
CD5_HUMAN T-CELL SURFACE GLYCOPROTEIN CD5 PRECURSOR (LYMPHOCYTE GLYCOPROTEIN T1/LEU-1) (LYMPHOCYTE ANTIGEN CD5) - HOMO SAPIENS (HUMAN).
CD5_MOUSE T-CELL SURFACE GLYCOPROTEIN CD5 PRECURSOR (LYMPHOCYTE GLYCOPROTEIN LY- 1) (LYT-1) (LYMPHOCYTE ANTIGEN CD5) - MUS MUSCULUS (MOUSE).
CD5_RAT T-CELL SURFACE GLYCOPROTEIN CD5 PRECURSOR (LYMPHOCYTE GLYCOPROTEIN LY- 1) (LYT-1) (LYMPHOCYTE ANTIGEN CD5) - RATTUS NORVEGICUS (RAT).

O73906 CD5 MRNA PRECURSOR - GALLUS GALLUS (CHICKEN).
Scan History
SPTR37_10f 1  50   NSINGLE    
Initial Motifs
Motif 1  width=23
Element Seqn Id St Int Rpt
CLEPQRTTPPPTTTPPTTVPEPT CD5_MOUSE 132 132 -
CLEPPRTTAPPTTSPPTTTPEPT CD5_BOVIN 133 133 -
CLEPQKTTPPTTRPPPTTTPEPT CD5_HUMAN 132 132 -

Motif 2 width=23
Element Seqn Id St Int Rpt
DLENFLCNNLQCGSFLKHLPETE CD5_HUMAN 195 40 -
GLGNLICKSLQCGSFLTHLSGTE CD5_MOUSE 195 40 -
DLGQLICAALQCGSFLKPLPETE CD5_BOVIN 196 40 -

Motif 3 width=22
Element Seqn Id St Int Rpt
PLPIRWEAPNGSCVSLQQCFQK CD5_MOUSE 232 14 -
PLPIRWEIQNPKCTSLEQCFRK CD5_BOVIN 230 11 -
PLPIQWKIQNSSCTSLEHCFRK CD5_HUMAN 232 14 -

Motif 4 width=26
Element Seqn Id St Int Rpt
CSDFQPKVQSRLVGGSSVCEGIAEVR CD5_MOUSE 267 13 -
CSGFQPKVQSRLVGGSSICEGTVEVR CD5_HUMAN 267 13 -
CSDFQPKVQSRLVGGSDVCEGSVEVR CD5_BOVIN 265 13 -

Motif 5 width=15
Element Seqn Id St Int Rpt
GFYCPPGILSRCHKL CD5_BOVIN 339 48 -
GFLCAQEKLSQCYHL CD5_MOUSE 338 45 -
GLFCPHQKLSQCHEL CD5_HUMAN 339 46 -

Motif 6 width=21
Element Seqn Id St Int Rpt
SIILTLVLLVVLLAMCGPLVY CD5_MOUSE 381 28 -
SIILALVLLVVLLVVCGPLAY CD5_HUMAN 382 28 -
SIILALLLLAVLLVVCGPLAY CD5_BOVIN 382 28 -

Motif 7 width=17
Element Seqn Id St Int Rpt
KKLVKKFRQKKQRQWIG CD5_HUMAN 403 0 -
KKVVKKFRQKKQRQWIG CD5_BOVIN 403 0 -
KKLVKKFRQKKQRQWIG CD5_MOUSE 402 0 -

Motif 8 width=17
Element Seqn Id St Int Rpt
TGMNQNMSFHRNHTVTV CD5_BOVIN 421 1 -
TGMNQNMSFHRNHTATV CD5_HUMAN 421 1 -
TGVNQNMSFHRSHTATV CD5_MOUSE 420 1 -

Motif 9 width=19
Element Seqn Id St Int Rpt
ENPTASHVDNEYSQPPRNS CD5_HUMAN 442 4 -
ENPTASHVDNEYSQPPRNS CD5_MOUSE 441 4 -
ENATASHVENEYSQPPRNS CD5_BOVIN 442 4 -

Motif 10 width=26
Element Seqn Id St Int Rpt
SAYPALEGALHRSSTQPDNSSDSDYD CD5_MOUSE 462 2 -
SAYPALEGALHRISTQPDNSSDSDYE CD5_BOVIN 463 2 -
SAYPALEGVLHRSSMQPDNSSDSDYD CD5_HUMAN 463 2 -
Final Motifs
Motif 1  width=23
Element Seqn Id St Int Rpt
CLEPQKTTPLPTTTLPTTMPEPT CD5_RAT 130 130 -
CLEPQRTTPPPTTTPPTTVPEPT CD5_MOUSE 132 132 -
CLEPPRTTAPPTTSPPTTTPEPT CD5_BOVIN 133 133 -
CLEPQKTTPPTTRPPPTTTPEPT CD5_HUMAN 132 132 -

Motif 2 width=23
Element Seqn Id St Int Rpt
DLGNLICKSLQCGSFLTHLSRIE CD5_RAT 193 40 -
GLGNLICKSLQCGSFLTHLSGTE CD5_MOUSE 195 40 -
DLGQLICAALQCGSFLKPLPETE CD5_BOVIN 196 40 -
DLENFLCNNLQCGSFLKHLPETE CD5_HUMAN 195 40 -

Motif 3 width=22
Element Seqn Id St Int Rpt
PLPIQWKIQNSSCTSLEHCFRK CD5_HUMAN 232 14 -
PLPIRWEAPNGSCVSLQQCFQK CD5_MOUSE 232 14 -
PLPIRWEIQNPKCTSLEQCFRK CD5_BOVIN 230 11 -
PLPIRWEAQNGSCTSLQQCFQK CD5_RAT 230 14 -

Motif 4 width=26
Element Seqn Id St Int Rpt
CSDFQPKVQSRLVGGSSVCEGIAEVR CD5_MOUSE 267 13 -
CSDFQPKVQSRLVGGSSVCEGIAEVR CD5_RAT 265 13 -
CSGFQPKVQSRLVGGSSICEGTVEVR CD5_HUMAN 267 13 -
CSDFQPKVQSRLVGGSDVCEGSVEVR CD5_BOVIN 265 13 -

Motif 5 width=15
Element Seqn Id St Int Rpt
GFLCAQEKLSQCYHL CD5_MOUSE 338 45 -
GVLCTQEKLSQCYQL CD5_RAT 336 45 -
GLFCPHQKLSQCHEL CD5_HUMAN 339 46 -
GFYCPPGILSRCHKL CD5_BOVIN 339 48 -

Motif 6 width=21
Element Seqn Id St Int Rpt
SIILALLLLAVLLVVCGPLAY CD5_BOVIN 382 28 -
SIILTLVLLVVLMVMCGPLIY CD5_RAT 378 27 -
SIILALVLLVVLLVVCGPLAY CD5_HUMAN 382 28 -
SIILTLVLLVVLLAMCGPLVY CD5_MOUSE 381 28 -

Motif 7 width=17
Element Seqn Id St Int Rpt
KKLVKKFRQKKQRQWIG CD5_HUMAN 403 0 -
KKLVKKFRQKKQRQWIG CD5_MOUSE 402 0 -
KKVVKKFRQKKQRQWIG CD5_BOVIN 403 0 -
KKLVKKFRQKKQRQWIG CD5_RAT 399 0 -

Motif 8 width=17
Element Seqn Id St Int Rpt
TGMNQNMSFHRNHTVTV CD5_BOVIN 421 1 -
TGVNQSMSFHRSHTATV CD5_RAT 417 1 -
TGMNQNMSFHRNHTATV CD5_HUMAN 421 1 -
TGVNQNMSFHRSHTATV CD5_MOUSE 420 1 -

Motif 9 width=19
Element Seqn Id St Int Rpt
ENPAASHVDNEYSQPPRNS CD5_RAT 438 4 -
ENPTASHVDNEYSQPPRNS CD5_MOUSE 441 4 -
ENATASHVENEYSQPPRNS CD5_BOVIN 442 4 -
ENPTASHVDNEYSQPPRNS CD5_HUMAN 442 4 -

Motif 10 width=26
Element Seqn Id St Int Rpt
SAYPALEGVLHRSSMQPDNSSDSDYD CD5_HUMAN 463 2 -
SAYPALEGALHRSSTQPDNSSDSDYD CD5_MOUSE 462 2 -
SAYPALEGALHRISTQPDNSSDSDYE CD5_BOVIN 463 2 -
SAYPALEGALHRSSTQPDNSSDSDYD CD5_RAT 459 2 -