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PR01407

Identifier
BUTYPHLNCDUF  [View Relations]  [View Alignment]  
Accession
PR01407
No. of Motifs
6
Creation Date
25-APR-2000
Title
Butyrophylin C-terminal DUF signature 
Database References
PRINTS; PR01406 BBOXZNFINGER
PFAM; PF00622 SPRY
INTERPRO; IPR000107
Literature References
1. BORDEN, K.L. 
RING fingers and B-boxes: zinc-binding protein-protein interaction domains. 
BIOCHEM.CELL BIOL. 76 351-358 (1998).
 
2. BORDEN, K.L., LALLY, J.M., MARTIN, S.R., O'REILLY, N.J., ETKIN, L.D.
AND FREEMONT, P.S.
Novel topology of a zinc-binding domain from a protein involved in
regulating early Xenopus development. 
EMBO J. 14(23) 5947-5956 (1995).
 
3. PONTING, C., SCHULTZ, J. AND BORK, P
SPRY domains in ryanodine receptors (Ca(2+)-release channels).
TRENDS BIOCHEM.SCI. 22 193-194 (1997).

Documentation
Several proteins that contain RING fingers also contain a well-conserved
40-residue cysteine-rich domain termed a B-box zinc finger. Often, one or 
two copies of the B-box are associated with a coiled coil domain in addition
to the ring finger, forming a tripartite motif. The tripartite motif is 
found in transcription factors, ribonucleoproteins and proto-oncoproteins,
but no function has yet been ascribed to the domain [1]. 
 
The solution structure of the B-box motif has been determined by NMR [2]. 
The protein is a monomer, with 2 beta-strands, 2 helical turns and 3
extended loop regions packed in a novel topology [2]. Of 7 potential zinc
ligands, only 4 are used, binding a single zinc atom in a C2-H2 tetrahedral 
arrangement [2]. The B-box structure differs in tertiary fold from all
other known zinc-binding motifs.
 
A group of proteins that contain the B-box motif also host a well conserved
domain of unknown function (DUF). Proteins that include this domain are,
e.g.: butyrophilin, the RET finger protein, the 52kDa Ro protein and the
Xenopus nuclear factor protein. The C-terminal portion of this region has
been termed the SPRY domain (after SPla and the RYanodine Receptor) [1].
 
BUTYPHLNCDUF is a 6-element fingerprint that provides a signature for the
butyrophilin C-terminal domain and related proteins. The fingerprint was
derived from an initial alignment of 7 proteins: the motifs were drawn from
short conserved regions spanning the C-terminal portion of the alignment -
motifs 1-3 lie N-terminal to the SPRY domain, and motifs 4-6 reside within
it. Five iterations on SPTR37_10f were required to reach convergence, at
which point a true set comprising 26 sequences was identified. Several
partial matches were also found, all of which are related DNA-binding
proteins that fail to match one or more motifs.
Summary Information
  26 codes involving  6 elements
0 codes involving 5 elements
1 codes involving 4 elements
2 codes involving 3 elements
7 codes involving 2 elements
Composite Feature Index
6262626262626
5000000
4001111
3102120
2206042
123456
True Positives
A33_PLEWA     BUTY_BOVIN    BUTY_HUMAN    BUTY_MOUSE    
O00475 O00478 O00480 O00481
O00635 O15553 O75656 O75657
O75658 O75677 O75678 O75679
O95604 P78408 P78409 Q12899
Q91431 Q92021 RFP_HUMAN RFP_MOUSE
RO52_HUMAN RO52_MOUSE
True Positive Partials
Codes involving 4 elements
Q91885
Codes involving 3 elements
O95361 Q91884
Codes involving 2 elements
O15344 O35418 O70583 Q14258
Q61510 Q91453 Y129_HUMAN
Sequence Titles
A33_PLEWA   ZINC-BINDING PROTEIN A33 - PLEURODELES WALTLII (IBERIAN RIBBED NEWT). 
BUTY_BOVIN BUTYROPHILIN PRECURSOR (BT) - BOS TAURUS (BOVINE).
BUTY_HUMAN BUTYROPHILIN PRECURSOR (BT) - HOMO SAPIENS (HUMAN).
BUTY_MOUSE BUTYROPHILIN PRECURSOR (BT) - MUS MUSCULUS (MOUSE).
O00475 BUTYROPHILIN - HOMO SAPIENS (HUMAN).
O00478 BUTYROPHILIN - HOMO SAPIENS (HUMAN).
O00480 BUTYROPHILIN (BTF2) (BUTYROPHILIN) - HOMO SAPIENS (HUMAN).
O00481 BUTYROPHILIN - HOMO SAPIENS (HUMAN).
O00635 SIMILAR TO THE 52KD RO/SSA PROTEIN AND RFP - HOMO SAPIENS (HUMAN).
O15553 PYRIN - HOMO SAPIENS (HUMAN).
O75656 DJ45P21.2 (BUTYROPHILIN) - HOMO SAPIENS (HUMAN).
O75657 BTF5 (BUTYROPHILIN) - HOMO SAPIENS (HUMAN).
O75658 BTF4 (BUTYROPHILIN) - HOMO SAPIENS (HUMAN).
O75677 RFPL1L - HOMO SAPIENS (HUMAN).
O75678 RET FINGER PROTEIN-LIKE 2 - HOMO SAPIENS (HUMAN).
O75679 RET FINGER PROTEIN-LIKE 3 - HOMO SAPIENS (HUMAN).
O95604 ZINC FINGER PROTEIN - HOMO SAPIENS (HUMAN).
P78408 BUTYROPHILIN PROTEIN - HOMO SAPIENS (HUMAN).
P78409 BUTYROPHILIN - HOMO SAPIENS (HUMAN).
Q12899 ACID FINGER PROTEIN - HOMO SAPIENS (HUMAN).
Q91431 NUCLEAR FACTOR 7 - XENOPUS LAEVIS (AFRICAN CLAWED FROG).
Q92021 XNF7=ZINC FINGER NUCLEAR PHOSPHOPROTEIN - XENOPUS LAEVIS (AFRICAN CLAWED FROG).
RFP_HUMAN ZINC-FINGER PROTEIN RFP (RET FINGER PROTEIN) - HOMO SAPIENS (HUMAN).
RFP_MOUSE ZINC-FINGER PROTEIN RFP (RET FINGER PROTEIN) - MUS MUSCULUS (MOUSE).
RO52_HUMAN 52 KD RO PROTEIN (SJOGREN SYNDROME TYPE A ANTIGEN (SS-A)) (RO(SS-A)) - HOMO SAPIENS (HUMAN).
RO52_MOUSE 52 KD RO PROTEIN (SJOGREN SYNDROME TYPE A ANTIGEN (SS-A)) (RO(SS-A)) (RO52) - MUS MUSCULUS (MOUSE).

Q91885 RING FINGER PROTEIN - XENOPUS LAEVIS (AFRICAN CLAWED FROG).

O95361 ESTROGEN-RESPONSIVE B BOX PROTEIN - HOMO SAPIENS (HUMAN).
Q91884 RING FINGER PROTEIN - XENOPUS LAEVIS (AFRICAN CLAWED FROG).

O15344 PUTATIVE TRANSCRIPTION FACTOR XPRF (MIDLINE 1 FETAL KIDNEY ISOFORM 3) - HOMO SAPIENS (HUMAN).
O35418 MIDLINE 1 (RING FINGER PROTEIN) - MUS MUSCULUS (MOUSE).
O70583 MIDLINE 1 (MIDLINE 1 PROTEIN) - MUS MUSCULUS (MOUSE).
Q14258 ESTROGEN RESPONSIVE FINGER PROTEIN (EFP) - HOMO SAPIENS (HUMAN).
Q61510 ESTROGEN-RESPONSIVE FINGER PROTEIN - MUS MUSCULUS (MOUSE).
Q91453 STONUSTOXIN BETA-SUBUNIT - SYNANCEIA HORRIDA.
Y129_HUMAN HYPOTHETICAL PROTEIN KIAA0129 - HOMO SAPIENS (HUMAN).
Scan History
SPTR37_10f 5  75   NSINGLE    
Initial Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
GLAPLTLDPNTAHPNLVL A33_PLEWA 444 444 -
HAVDVTLDPDTAHPHLFL BUTY_HUMAN 301 301 -
YSVDVTLDPDTAYPSLIL RFP_MOUSE 323 323 -
YSVDVTLDPDTAYPSLIL RFP_HUMAN 314 314 -
CAVHITLDPDTANPWLIL RO52_HUMAN 285 285 -
HAVDVTLDPDTAHPHLFL BUTY_BOVIN 301 301 -
HEVDVTLDPDTAHPHLFL BUTY_MOUSE 302 302 -

Motif 2 width=18
Element Seqn Id St Int Rpt
LYEDSKSVRLEDSRQKLP BUTY_BOVIN 318 -1 -
LYEDSKSVRLEDSRQILP BUTY_MOUSE 319 -1 -
LYEDSKSVRLEDSRQKLP BUTY_HUMAN 318 -1 -
LSEGLTSVKYTDTKQQLP A33_PLEWA 461 -1 -
LSDNLRQVRYSYLQQDLP RFP_MOUSE 340 -1 -
LSDNLRQVRYSYLQQDLP RFP_HUMAN 331 -1 -
LSEDRRQVRLGDTQQSIP RO52_HUMAN 302 -1 -

Motif 3 width=25
Element Seqn Id St Int Rpt
RFSQCILVLGAEGFDSGKHYWEVEV A33_PLEWA 483 4 -
RFDSWPCVLGRETFTSGRHYWEVEV BUTY_HUMAN 340 4 -
RFDSWPCVLGRETFTSGRHYWEVEV BUTY_MOUSE 341 4 -
RFDSWPCVMGREAFTSGRHYWEVEV BUTY_BOVIN 340 4 -
RFDSYPMVLGAQHFHSGKHYWEVDV RO52_HUMAN 324 4 -
RFNLFPCVLGSPCFIAGRHYWEVEV RFP_HUMAN 353 4 -
RFNLFPCVLGSPCFIAGRHYWEVEV RFP_MOUSE 362 4 -

Motif 4 width=14
Element Seqn Id St Int Rpt
WAIGVCRENVVKKG BUTY_MOUSE 371 5 -
WAIGVCRENVMKKG BUTY_HUMAN 370 5 -
WAIGVCRENVMKKG BUTY_BOVIN 370 5 -
WDLGVCRDSVRRKG RO52_HUMAN 354 5 -
WTIGVCEDSVCRKG RFP_HUMAN 383 5 -
WTIGVCEDSVCRKG RFP_MOUSE 392 5 -
WDVGMASESSNRKG A33_PLEWA 513 5 -

Motif 5 width=25
Element Seqn Id St Int Rpt
LPLRTPLQRVGIFLDYDAGEVSFYN RFP_MOUSE 436 30 -
LPLRTPLQRVGIFLDYDAGEVSFYN RFP_HUMAN 427 30 -
LHLQVPPCQVGIFLDYEAGMVSFYN RO52_HUMAN 398 30 -
LPLAGPPRRVGVFLDYESGDIFFYN BUTY_BOVIN 413 29 -
LRLAGPPRRVGVFLDYDAGDISFYN BUTY_MOUSE 414 29 -
LPLAGPPRRVGIFLDYESGDISFYN BUTY_HUMAN 413 29 -
LNLTSKPSKIGVYLDYEGGQVSFYN A33_PLEWA 557 30 -

Motif 6 width=19
Element Seqn Id St Int Rpt
PIYTFNGSFTEKLYPYLSP A33_PLEWA 587 5 -
TFTFSHATFCGPVRPYFSL RFP_MOUSE 467 6 -
TFTFSHATFCGPVRPYFSL RFP_HUMAN 458 6 -
IYSFSECAFTGPLRPFFSP RO52_HUMAN 430 7 -
IYTFSKASFSGPLRPFFCL BUTY_BOVIN 444 6 -
IYTFPSISFSGPLRPFFCL BUTY_MOUSE 445 6 -
IYTFSNVTFSGPLRPFFCL BUTY_HUMAN 444 6 -
Final Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
FQVDMTLDADTANNFLLI O75679 94 94 -
KTVSVTLDPQSASGYLQL Q12899 311 311 -
DSGVITLDPQTASRSLVL O95604 292 292 -
SLTPMLLDPTSAHPNLHL Q92021 430 430 -
SLTPMLLDPNSAHPNLHL Q91431 431 431 -
GLAPLTLDPNTAHPNLVL A33_PLEWA 444 444 -
FQVDMTLDANTANNFLLI O75678 94 94 -
HAVDVTLDPDTAHPHLFL BUTY_HUMAN 301 301 -
CWVHITLDRNTANSWLII RO52_MOUSE 289 289 -
FQVDMTLDADTANNFLLI O75677 94 94 -
HAVNVILDAETAYPNLIF O15553 596 596 -
HQVSVTLDPDTAHHELIL O00635 290 290 -
HAANVVLDQDTGHPYLFV O75656 243 243 -
LTPDVILYPDMANAILLV O75658 311 311 -
SAADVILYPDMANAILLV P78409 496 496 -
YSVDVTLDPDTAYPSLIL RFP_MOUSE 323 323 -
YSVDVTLDPDTAYPSLIL RFP_HUMAN 314 314 -
HAVDVVLDPDTAHPDLFL P78408 312 312 -
KPADVILDPKTANPILLV O75657 320 320 -
KPADVILDPKTANPILLV O00481 338 338 -
CAVHITLDPDTANPWLIL RO52_HUMAN 285 285 -
KPADVILDPDTANAILLV O00478 338 338 -
HAVDVVLDPDTAHPDLFL O00475 326 326 -
HAVDVTLDPDTAHPHLFL BUTY_BOVIN 301 301 -
HAADVVLDPDTAHPELFL O00480 325 325 -
HEVDVTLDPDTAHPHLFL BUTY_MOUSE 302 302 -

Motif 2 width=18
Element Seqn Id St Int Rpt
VSEDQRSVQRAKEPQDLP O75657 337 -1 -
LSEDRRQVRLGDTQQSIP RO52_HUMAN 302 -1 -
VSEDQRSVQRAEEPRDLP O00478 355 -1 -
LSEDRRSVRRCPFRHLGE O00475 343 -1 -
LYEDSKSVRLEDSRQKLP BUTY_BOVIN 318 -1 -
LSEDRRSVRRGPYRQRVP O00480 342 -1 -
LYEDSKSVRLEDSRQILP BUTY_MOUSE 319 -1 -
LYEDSKSVRLEDSRQKLP BUTY_HUMAN 318 -1 -
LSEDWKCVTYTSLYKSAY Q12899 328 -1 -
LSEDRKSVRYTRQKKSLP O95604 309 -1 -
LSDGLTSVRYGENKLSLP Q92021 447 -1 -
LSDGLTSVRYGENKLSLP Q91431 448 -1 -
LSEGLTSVKYTDTKQQLP A33_PLEWA 461 -1 -
ISDDLRSVRSGRIRQNRQ O75678 111 -1 -
ISDDLRSVRSGLITQNRQ O75679 111 -1 -
ISKDRRQVRMGDTHQNVS RO52_MOUSE 306 -1 -
ISDDLRSVRSGCITQNRQ O75677 111 -1 -
FSDDLKSVRLGNKWERLP O15553 613 -1 -
LSEDRRQVTRGYTQENQD O00635 307 -1 -
VSEDKRSVTLDPSRESIP O75656 260 -1 -
VSEDQRSVQRAEEPHDLP O75658 328 -1 -
VSEDQRSVQRAEEPHDLP P78409 513 -1 -
LSDNLRQVRYSYLQQDLP RFP_MOUSE 340 -1 -
LSDNLRQVRYSYLQQDLP RFP_HUMAN 331 -1 -
LSEDRRSVRRCPFRHLGE P78408 329 -1 -
VSEDQRSVQRAKEPQDLP O00481 355 -1 -

Motif 3 width=25
Element Seqn Id St Int Rpt
RFDVSICILGSPRFTCGRHYWEVDV O75677 133 4 -
RFSNYPMVLGAQRFSSGKMYWEVDV RO52_MOUSE 328 4 -
RFDVSVCILGSPRFTCGRHYWEVDV O75679 133 4 -
RFDVSVCILGSPRFTCGRHCWEVDV O75678 133 4 -
RFSQCILVLGAEGFDSGKHYWEVEV A33_PLEWA 483 4 -
RFSQCILVLGSQGFDSGRHYWEVEV Q91431 470 4 -
RFSQCILVLGSQGFDSGRHYWEVEV Q92021 469 4 -
RFDGLPAVLGFPGFSSGRHRWQVDL O95604 331 4 -
QFDCEPGVLGSKGFTWGKVYWEVEV Q12899 350 4 -
RFDSWPCVLGRETFTSGRHYWEVEV BUTY_HUMAN 340 4 -
RFDSWPCVLGRETFTSGRHYWEVEV BUTY_MOUSE 341 4 -
RFDSQPCVLGWESFASGKHYWEVEV O00480 364 4 -
RFDSWPCVMGREAFTSGRHYWEVEV BUTY_BOVIN 340 4 -
RFDSQPCVLGRESFASGKHYWEVEV O00475 368 7 -
RFEWRYCVLGCENFTSGRHYWEVEV O00478 377 4 -
RFDSYPMVLGAQHFHSGKHYWEVDV RO52_HUMAN 324 4 -
RFNWHYCVLGCESFISGRHYWEVEV O00481 377 4 -
RFNWHYCVLGCESFISGRHYWEVEV O75657 359 4 -
RFDSQPCVLGRESFASGKHYWEVEV P78408 354 7 -
RFNLFPCVLGSPCFIAGRHYWEVEV RFP_HUMAN 353 4 -
RFNLFPCVLGSPCFIAGRHYWEVEV RFP_MOUSE 362 4 -
RFEWRYCVLGCESFMSERHYWEVEV P78409 535 4 -
RFEWRYCVLGCESFMSERHYWEVEV O75658 350 4 -
RFDSQLCVLGQESFASGKHYLEVDV O75656 282 4 -
RFTAFPCVLGCEGFTSGRRYFEVDV O00635 329 4 -
RFDSCIIVLGSPSFLSGRRYWEVEV O15553 635 4 -

Motif 4 width=14
Element Seqn Id St Int Rpt
WDLGVCRESVHCKG O75679 163 5 -
WDLGVCRDSVQRKG RO52_MOUSE 358 5 -
WAIGVCRENVMKKG BUTY_HUMAN 370 5 -
WAIGVCRENVVKKG BUTY_MOUSE 371 5 -
WTVGVCRHSVERKG O00480 394 5 -
WAIGVCRENVMKKG BUTY_BOVIN 370 5 -
WTVGVCRDSVERKG O00475 398 5 -
WHIGVCSKNVERKK O00478 407 5 -
WDLGVCRDSVRRKG RO52_HUMAN 354 5 -
WHIGVCSKNVQRKG O00481 407 5 -
WHIGVCSKNVQRKG O75657 389 5 -
WTVGVCRDSVERKG P78408 384 5 -
WTIGVCEDSVCRKG RFP_HUMAN 383 5 -
WTIGVCEDSVCRKG RFP_MOUSE 392 5 -
WHIGVCSKNVERKK P78409 565 5 -
WHIGVCSKNVERKK O75658 380 5 -
WTVGICRDNVERKW O75656 312 5 -
WDLGVCMENVQRGT O00635 359 5 -
WILGACKTSISRKG O15553 665 5 -
WDLGVCRESVHRKG O75677 163 5 -
WDLGVCRESVHRKG O75678 163 5 -
WDVGMASESSNRKG A33_PLEWA 513 5 -
WDVGMASESSNRKG Q91431 500 5 -
WDVGMASESSNRKG Q92021 499 5 -
CTVGVAGEGVRRKG O95604 363 7 -
CMVGVARDSVKRKG Q12899 437 62 -

Motif 5 width=25
Element Seqn Id St Int Rpt
LKLPEPPRKVGVILDYETGHISFYN P78409 610 31 -
LPLRTPLQRVGIFLDYDAGEVSFYN RFP_MOUSE 436 30 -
LPLRTPLQRVGIFLDYDAGEVSFYN RFP_HUMAN 427 30 -
LPLKESLCRVGVFLDYEAGDVSFYN P78408 427 29 -
LKLPKPPKKVGVFLDYETGDISFYN O75657 433 30 -
LKLPKPPKKVGVFLDYETGDISFYN O00481 451 30 -
LHLQVPPCQVGIFLDYEAGMVSFYN RO52_HUMAN 398 30 -
LKLPEPPRKVGIFLDYETGEISFYN O00478 452 31 -
LPLKESLCRVGVFLDYEAGDVSFYN O00475 441 29 -
LPLAGPPRRVGVFLDYESGDIFFYN BUTY_BOVIN 413 29 -
LPLKESLCRVGVFLDYEAGDVSFYN O00480 437 29 -
LRLAGPPRRVGVFLDYDAGDISFYN BUTY_MOUSE 414 29 -
LPLAGPPRRVGIFLDYESGDISFYN BUTY_HUMAN 413 29 -
LHIQVPPCQIGIFVDYEAGVVSFYN RO52_MOUSE 401 29 -
LFVDRKLQRVGIFLDMGMQNVSFFD O75677 207 30 -
LLIKEPPKRVGIFVDYRVGSISFYN O15553 709 30 -
LHLHEQPLLVGIFLDYEAGVVSFYN O00635 403 30 -
LSLEEPLCQVGIFLDYEAGDVSFYN O75656 355 29 -
LKLPEPPRKVGVILDYETGHISFYN O75658 425 31 -
LLVDRKLQRVGIFLDMGMQNVSFFD O75679 207 30 -
LFPALRPRRVGIALDYEGGTVTFTN Q12899 480 29 -
LPLSEIPRGVRVALDYEAGQVTLHN O95604 406 29 -
LSLSSHPRKIGVYVDYEGGQISFYN Q92021 543 30 -
LSLTSHPRKIGVYVDYEGGQISFYN Q91431 544 30 -
LNLTSKPSKIGVYLDYEGGQVSFYN A33_PLEWA 557 30 -
LFVDRKLQRVGIFLDMGMQNVSFFD O75678 207 30 -

Motif 6 width=19
Element Seqn Id St Int Rpt
IYTFSNVTFSGPLRPFFCL BUTY_HUMAN 444 6 -
LIYTFTATFTRRLVPFLWL Q12899 510 5 -
PIFTFTASFSGKVFPFFAV O95604 436 5 -
IIYTFNATFTEKLYPYLSP Q92021 573 5 -
IIYTFSATFTEKLYPYLSP Q91431 574 5 -
PIYTFNGSFTEKLYPYLSP A33_PLEWA 587 5 -
VYTFRSVSAEEPLCPFLAP O75678 238 6 -
VYTFRSVSAEEPLRPFLAP O75679 238 6 -
IYTFSECVFAGPLRPFFNV RO52_MOUSE 433 7 -
VYTFRSVSAEEPLHLFFAP O75677 238 6 -
IYTFASCSFSGPLQPIFSP O15553 740 6 -
IFTFPKASFSDTLRPYFQV O00635 434 6 -
IYTFPHSAFSVPVRPFFSL O75656 386 6 -
IYTFLHASSSEPLYPVFRI O75658 456 6 -
IYTFLHASSSEPLYPVFRI P78409 641 6 -
TFTFSHATFCGPVRPYFSL RFP_MOUSE 467 6 -
TFTFSHATFCGPVRPYFSL RFP_HUMAN 458 6 -
IYTCPRSAFSGPDTSQSGD P78408 458 6 -
IHTFLDVSFSEALYPVFRI O75657 464 6 -
IHTFLDVSFSEALYPVFRI O00481 482 6 -
IYSFSECAFTGPLRPFFSP RO52_HUMAN 430 7 -
IYTFPHASFSEPLYPVFRI O00478 483 6 -
IYTCPRSAFSVPVRPFFRL O00475 472 6 -
IYTFSKASFSGPLRPFFCL BUTY_BOVIN 444 6 -
IYTCPRSAFTVPVRPFFRL O00480 468 6 -
IYTFPSISFSGPLRPFFCL BUTY_MOUSE 445 6 -