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PR01294

Identifier
INTRLEUKIN10  [View Relations]  [View Alignment]  
Accession
PR01294
No. of Motifs
4
Creation Date
08-MAR-2000
Title
Interleukin-10 signature
Database References

PROSITE; PS00520 INTERLEUKIN_10
BLOCKS; BL00520
PFAM; PF00726 IL10
PDB; 1ILK
SCOP; 1ILK
CATH; 1ILK
Literature References
1. NICOLA N.A.
Interleukin-10 (IL-10).
IN GUIDEBOOK TO CYTOKINES AND THEIR RECEPTORS (NEW YORK), 1994, PP.83-85
 
2. ZDANOV A., SCHALK-HIHI C., GUSTCHINA A., TSANG M., WEATHERBEE J.
AND WLODAWER A.
Crystal structure of interleukin-10 reveals the functional dimer with an
unexpected topological similarity to interferon gamma.
STRUCTURE 3 591-601 (1995).

Documentation
Interleukin 10 (IL-10) is a secreted 17-21kDa protein that is non-
glycosylated in humans but exists as a non-covalent homodimer. IL-10 is
secreted by some types of T-lymphocyte, B-lymphocytes and macrophages late
after antigen activation.
 
Generally, IL-10 inhibits T-cell and natural killer cell synthesis of other
cytokines at the transcriptional level, indirectly, by affecting
macrophages. These cytokines include interferon-gamma, IL-2, IL-3, tumour
necrosis factor and granulocyte-macrophage colony-stimulating factor
(GM-CSF). The mechanism of IL-10 induced inhibition appears to involve a
reduction in the abundance of the macrophage surface molecules that directly
stimulate T-cells and natural killer cells [1].
 
Proteins encoded by viruses such as Epstein-Barr virus and equine herpes
virus show a high degree of sequence identity to IL-10. These proteins are
thought to be involved in evasion of host immune responses. The IL-10 
molecule is a tight dimer of 2 interpenetrating subunits that form a 
V-shaped structure. Each half of the structure consists of 6 alpha-helices
(4 from 1 subunit and 2 from the other). Four of the helices form an 
up-up-down-down bundle, which is observed in all other helical cytokines.
The overall topology resembles interferon-gamma [2].
 
INTRLEUKIN10 is a 4-element fingerprint that provides a signature for 
interleukin-10. The fingerprint was derived from an initial alignment of 6
sequences: the motifs were drawn from conserved regions spanning the full
alignment length - motif 1 spans alpha-helix 1; motif 2 contains helix 3 and
the preceding loop; motif 3 contains helix 7 and the preceding loop; and
motif 4 spans helix 9 and the N-terminal portion of helix 10. Two iterations
on SPTR37_10f were required to reach convergence, at which point a true set
comprising 21 sequences was identified.
Summary Information
21 codes involving  4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
421212121
30000
20000
1234
True Positives
BCRF_EBV      I10H_HSVE2    IL10_BOVIN    IL10_CANFA    
IL10_CEREL IL10_CERTO IL10_FELCA IL10_HORSE
IL10_HUMAN IL10_MACFA IL10_MACMU IL10_MACNE
IL10_MERUN IL10_MOUSE IL10_PIG IL10_RAT
IL10_SHEEP O11386 O11387 O97798
Q9Z1Y5
Sequence Titles
BCRF_EBV    BCRF1 PROTEIN PRECURSOR (20 KD PROTEIN) - EPSTEIN-BARR VIRUS (STRAIN B95-8) (HUM 
I10H_HSVE2 INTERLEUKIN-10 HOMOLOG PRECURSOR - EQUINE HERPESVIRUS TYPE 2 (STRAIN 86/87) (EHV
IL10_BOVIN INTERLEUKIN-10 PRECURSOR (IL-10) (CYTOKINE SYNTHESIS INHIBITORY FACTOR) (CSIF) -
IL10_CANFA INTERLEUKIN-10 PRECURSOR (IL-10) (CYTOKINE SYNTHESIS INHIBITORY FACTOR) (CSIF) -
IL10_CEREL INTERLEUKIN-10 PRECURSOR (IL-10) (CYTOKINE SYNTHESIS INHIBITORY FACTOR) (CSIF) -
IL10_CERTO INTERLEUKIN-10 PRECURSOR (IL-10) (CYTOKINE SYNTHESIS INHIBITORY FACTOR) (CSIF) -
IL10_FELCA INTERLEUKIN-10 PRECURSOR (IL-10) (CYTOKINE SYNTHESIS INHIBITORY FACTOR) (CSIF) -
IL10_HORSE INTERLEUKIN-10 PRECURSOR (IL-10) (CYTOKINE SYNTHESIS INHIBITORY FACTOR) (CSIF) -
IL10_HUMAN INTERLEUKIN-10 PRECURSOR (IL-10) (CYTOKINE SYNTHESIS INHIBITORY FACTOR) (CSIF) -
IL10_MACFA INTERLEUKIN-10 PRECURSOR (IL-10) (CYTOKINE SYNTHESIS INHIBITORY FACTOR) (CSIF) -
IL10_MACMU INTERLEUKIN-10 PRECURSOR (IL-10) (CYTOKINE SYNTHESIS INHIBITORY FACTOR) (CSIF) -
IL10_MACNE INTERLEUKIN-10 PRECURSOR (IL-10) (CYTOKINE SYNTHESIS INHIBITORY FACTOR) (CSIF) -
IL10_MERUN INTERLEUKIN-10 PRECURSOR (IL-10) (CYTOKINE SYNTHESIS INHIBITORY FACTOR) (CSIF) -
IL10_MOUSE INTERLEUKIN-10 PRECURSOR (IL-10) (CYTOKINE SYNTHESIS INHIBITORY FACTOR) (CSIF) -
IL10_PIG INTERLEUKIN-10 PRECURSOR (IL-10) (CYTOKINE SYNTHESIS INHIBITORY FACTOR) (CSIF) -
IL10_RAT INTERLEUKIN-10 PRECURSOR (IL-10) (CYTOKINE SYNTHESIS INHIBITORY FACTOR) (CSIF) -
IL10_SHEEP INTERLEUKIN-10 PRECURSOR (IL-10) (CYTOKINE SYNTHESIS INHIBITORY FACTOR) (CSIF) -
O11386 INTERLEUKIN-10 - ORF VIRUS.
O11387 PUTATIVE INTERLEUKIN-10 - ORF VIRUS.
O97798 INTERLEUKIN-10 - TRICHOSURUS VULPECULA (BRUSH-TAILED POSSUM).
Q9Z1Y5 INTERLEUKIN 10 - CAVIA PORCELLUS (GUINEA PIG).
Scan History
SPTR37_10f 2  200  NSINGLE    
Initial Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
MLRDLRDAFSRVKTFFQ IL10_MACNE 40 40 -
MLRDLRDAFSRVKTFFQ BCRF_EBV 33 33 -
MLRDLRDAFSRVKTFFQ IL10_HUMAN 40 40 -
MLRELRAAFGRVKIFFQ IL10_CANFA 41 41 -
MLLELRTAFSQVKTFFQ IL10_MOUSE 40 40 -
MLRELRAAFSSVKTYFQ O97798 36 36 -

Motif 2 width=17
Element Seqn Id St Int Rpt
KGYLGCQALSEMIQFYL IL10_MACNE 75 18 -
KGYLGCQALSEMIQFYL BCRF_EBV 68 18 -
KGYLGCQALSEMIQFYL IL10_HUMAN 75 18 -
KSYLGCQALSEMIQFYL IL10_CANFA 76 18 -
KGYLGCQALSEMIQFYL IL10_MOUSE 75 18 -
KSYLGCQALSEMIKFYL O97798 71 18 -

Motif 3 width=10
Element Seqn Id St Int Rpt
RRCHRFLPCE IL10_MACNE 124 32 -
RRCHRFLPCE BCRF_EBV 117 32 -
RRCHRFLPCE IL10_HUMAN 124 32 -
RRCHRFLPCE IL10_CANFA 127 34 -
RRCHRFLKCE IL10_MOUSE 124 32 -
KRCHRFLPCE O97798 120 32 -

Motif 4 width=17
Element Seqn Id St Int Rpt
LQEKGVYKAMSEFDIFI IL10_MACNE 149 15 -
LQEKGIYKAMSEFDIFI BCRF_EBV 142 15 -
LQEKGIYKAMSEFDIFI IL10_HUMAN 149 15 -
LQEKGVYKAMSEFDIFI IL10_CANFA 152 15 -
LEDQGVYKAMNEFDIFI IL10_MOUSE 149 15 -
LQEQGVYKAMGDFDIFI O97798 145 15 -
Final Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
MLRDLRDAFSRVKTFFQ IL10_MACFA 40 40 -
MLRDLRDAFSRVKTFFQ IL10_MACNE 40 40 -
MLRDLRDAFSRVKTFFQ IL10_MACMU 40 40 -
MLHELRAAFSRVKTFFQ I10H_HSVE2 40 40 -
MLHELRAAFSRVKTFFQ IL10_HORSE 40 40 -
MLRELRAAFSQVKTFFQ IL10_MERUN 40 40 -
MLRELRAAFGKVKTFFQ O11386 50 50 -
MLRELRAAFGKVKTFFQ O11387 50 50 -
MLRDLRDAFSRVKTFFQ BCRF_EBV 33 33 -
MLRDLRDAFSRVKTFFQ IL10_HUMAN 40 40 -
MLRDLRDVFSRVKTFFQ IL10_CERTO 40 40 -
MLRELRTAFSRVKNFFQ IL10_CEREL 41 41 -
MLRELRAAFGRVKTFFQ Q9Z1Y5 40 40 -
MLRELRAAFGRVKIFFQ IL10_CANFA 41 41 -
MLXELRAAFGKVKTFFQ IL10_FELCA 40 40 -
MLRELRAAFGEAKTFFQ IL10_BOVIN 40 40 -
MLRDVRAAFGKVKTFFQ IL10_SHEEP 41 41 -
MLRELRAAFSQVKTFFQ IL10_RAT 40 40 -
MLRELRAAFGPVKSFFQ IL10_PIG 36 36 -
MLLELRTAFSQVKTFFQ IL10_MOUSE 40 40 -
MLRELRAAFSSVKTYFQ O97798 36 36 -

Motif 2 width=17
Element Seqn Id St Int Rpt
KGYLGCQALSEMIQFYL IL10_MACFA 75 18 -
KGYLGCQALSEMIQFYL IL10_MACNE 75 18 -
KGYLGCQALSEMIQFYL IL10_MACMU 75 18 -
KGYLGCQALSEMIQFYL I10H_HSVE2 75 18 -
KGYLGCQALSEMIQFYL IL10_HORSE 75 18 -
KGYLGCQALSEMIQFYL IL10_MERUN 75 18 -
KGYLGCQALSEMIQFYL O11386 85 18 -
KGYLGCQALSEMIQFYL O11387 85 18 -
KGYLGCQALSEMIQFYL BCRF_EBV 68 18 -
KGYLGCQALSEMIQFYL IL10_HUMAN 75 18 -
KGYLGCQALSEMIQFYL IL10_CERTO 75 18 -
KGYLGCQALSEMIQFYL IL10_CEREL 76 18 -
KGYLGCQALSEMIQFYL Q9Z1Y5 75 18 -
KSYLGCQALSEMIQFYL IL10_CANFA 76 18 -
KGYLGCQALSEMIQFYL IL10_FELCA 75 18 -
KGYLGCQALSEMIQFYL IL10_BOVIN 75 18 -
KGYLGCQALSEMIQFYL IL10_SHEEP 76 18 -
KGYLGCQALSEMIKFYL IL10_RAT 75 18 -
KGYLGCQALSEMIQFYL IL10_PIG 71 18 -
KGYLGCQALSEMIQFYL IL10_MOUSE 75 18 -
KSYLGCQALSEMIKFYL O97798 71 18 -

Motif 3 width=10
Element Seqn Id St Int Rpt
RRCHRFLPCE IL10_MACFA 124 32 -
RRCHRFLPCE IL10_MACNE 124 32 -
RRCHRFLPCE IL10_MACMU 124 32 -
RRCHRFLPCE I10H_HSVE2 125 33 -
RRCHRFLPCE IL10_HORSE 124 32 -
RRCHRFLPCE IL10_MERUN 124 32 -
RRCHRFLPCE O11386 134 32 -
RRCHRFLPCE O11387 134 32 -
RRCHRFLPCE BCRF_EBV 117 32 -
RRCHRFLPCE IL10_HUMAN 124 32 -
RRCHRFLPCE IL10_CERTO 124 32 -
RRCHRFLPCE IL10_CEREL 125 32 -
RRCHRFLPCE Q9Z1Y5 124 32 -
RRCHRFLPCE IL10_CANFA 127 34 -
RRCHRFLPCE IL10_FELCA 124 32 -
RRCHRFLPCE IL10_BOVIN 124 32 -
RRCHRFLPCE IL10_SHEEP 125 32 -
RRCHRFLPCE IL10_RAT 124 32 -
RRCHQFLPCE IL10_PIG 120 32 -
RRCHRFLKCE IL10_MOUSE 124 32 -
KRCHRFLPCE O97798 120 32 -

Motif 4 width=17
Element Seqn Id St Int Rpt
LQEKGVYKAMSEFDIFI IL10_MACFA 149 15 -
LQEKGVYKAMSEFDIFI IL10_MACNE 149 15 -
LQEKGVYKAMSEFDIFI IL10_MACMU 149 15 -
LQEKGVYKAMSEFDIFI I10H_HSVE2 150 15 -
LQEKGVYKAMSEFDIFI IL10_HORSE 149 15 -
LQEKGVYKAMNEFDIFI IL10_MERUN 149 15 -
LQERGVYKAMSEFDIFI O11386 159 15 -
LQERGVYKAMSEFDIFI O11387 159 15 -
LQEKGIYKAMSEFDIFI BCRF_EBV 142 15 -
LQEKGIYKAMSEFDIFI IL10_HUMAN 149 15 -
LQEKGVYKAMSEFDIFI IL10_CERTO 149 15 -
LQERGVYKAMSEFDIFI IL10_CEREL 150 15 -
LQDQGVYKAMNEFDIFI Q9Z1Y5 149 15 -
LQEKGVYKAMSEFDIFI IL10_CANFA 152 15 -
LQEKGVYKAMGEFDIFI IL10_FELCA 149 15 -
LQERGVYKAMSEFDIFI IL10_BOVIN 149 15 -
LQERGVYKAMSEFDIFI IL10_SHEEP 150 15 -
LQDKGVYKAMNEFDIFI IL10_RAT 149 15 -
LQERGVYKAMGEFDIFI IL10_PIG 145 15 -
LEDQGVYKAMNEFDIFI IL10_MOUSE 149 15 -
LQEQGVYKAMGDFDIFI O97798 145 15 -