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PR01143

Identifier
FSHRECEPTOR  [View Relations]  [View Alignment]  
Accession
PR01143
No. of Motifs
10
Creation Date
12-MAR-1999
Title
Follicle stimulating hormone receptor precursor signature 
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR00373 GLYCHORMONER
INTERPRO; IPR002272
GCRDB; GCR_0766; GCR_0071; GCR_0404; GCR_0588; GCR_0690; GCR_1251

GCRDB; GCR_1561
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL, S.
Glycoprotein hormones.
In THE G-PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, pp142-149.
 
7. JIANG, X., DREANO, M., BUCKLER, D.R., CHENG, S., YTHIER, A., WU, H.,
HENDRICKSON, W.A. AND EL TAYAR, N.
Structural predictions for the ligand-binding region of glycoprotein hormone
receptors and the nature of hormone-receptor interactions.
STRUCTURE 3 1341-1353 (1995).

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
The gonadotrophins (luteinising hormone, choriogonadotrophin, follicle-
stimulating hormone) and thyroid-stimulating hormone are heterotrimeric
glycoproteins composed of a common alpha-subunit and distinct beta-subunits
[6]. The carbohydrate moiety of the glycoproteins has an essential role in
receptor recognition. The receptors share close sequence similarity, and
are characterised by large extracellular domains believed to be involved
in hormone binding via leucine-rich repeats (LRR) [7]. Modelling of this
portion of the receptors suggests that they contain three-dimensional
structures similar to that of porcine ribonuclease inhibitor [7].
 
FSH is released from the anterior pituitary under the influence of
gonadotrophin-releasing hormone and oestrogens, and from the placenta during
pregnancy [6]. In females, it acts on the ovaries, promoting development
of follicles and is the principal hormone regulating secretion of
oestrogens [6]. In males, it is responsible for the integrity of the
seminiferous tubules and acts on Sertoli cells to support gametogenesis [6].
FSH is used clinically to treat infertility in females and for some types
of spermatogenesis in males [6]. The receptor is found in ovary and testis,
its predominant effector pathway being via activation of adenylyl cyclase
through Gs [6].
 
FSHRECEPTOR is a 10-element fingerprint that provides a signature for the
follicle stimulating hormone receptors. The fingerprint was derived from an
initial alignment of 9 sequences: the motifs were drawn from conserved 
regions spanning virtually the full alignment length, focusing on those 
sections that characterise the FSH receptors but distinguish them from the
rest of the glycoprotein hormone receptor family - motifs 1-9 reside within
the N-terminal half of the alignment, prior to the TM domains; and motif 10
lies at the C-terminus. Two iterations on SPTR37_9f were required to reach 
convergence, at which point a true set comprising 10 sequences was identfied.
Two partial matches were also found, Q28574 and Q28573, both of which are
fragments.
Summary Information
  10 codes involving 10 elements
0 codes involving 9 elements
0 codes involving 8 elements
1 codes involving 7 elements
0 codes involving 6 elements
1 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
1010101010101010101010
90000000000
80000000000
71111111000
60000000000
51111100000
40000000000
30000000000
20000000000
12345678910
True Positives
FSHR_BOVIN    FSHR_CHICK    FSHR_EQUAS    FSHR_HORSE    
FSHR_HUMAN FSHR_MACFA FSHR_PIG FSHR_RAT
FSHR_SHEEP Q64183
True Positive Partials
Codes involving 7 elements
Q28574
Codes involving 5 elements
Q28573
Sequence Titles
FSHR_BOVIN  Follicle-stimulating hormone receptor precursor (FSH-R) (Follitropin receptor) - Bos taurus (Bovine). 
FSHR_CHICK Follicle-stimulating hormone receptor precursor (FSH-R) (Follitropin receptor) - Gallus gallus (Chicken).
FSHR_EQUAS Follicle-stimulating hormone receptor precursor (FSH-R) (Follitropin receptor) - Equus asinus (Donkey).
FSHR_HORSE Follicle-stimulating hormone receptor precursor (FSH-R) (Follitropin receptor) - Equus caballus (Horse).
FSHR_HUMAN Follicle-stimulating hormone receptor precursor (FSH-R) (Follitropin receptor) - Homo sapiens (Human).
FSHR_MACFA Follicle-stimulating hormone receptor precursor (FSH-R) (Follitropin receptor) - Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey).
FSHR_PIG Follicle-stimulating hormone receptor precursor (FSH-R) (Follitropin receptor) - Sus scrofa (Pig).
FSHR_RAT Follicle-stimulating hormone receptor precursor (FSH-R) (Follitropin receptor) - Rattus norvegicus (Rat).
FSHR_SHEEP Follicle-stimulating hormone receptor precursor (FSH-R) (Follitropin receptor) - Ovis aries (Sheep).
Q64183 FOLLICLE-STIMULATING HORMONE RECEPTOR - RATTUS NORVEGICUS (RAT).

Q28574 FOLLITROPIN RECEPTOR PRECURSOR - OVIS ARIES (SHEEP).

Q28573 FOLLITROPIN RECEPTOR PRECURSOR - OVIS ARIES (SHEEP).
Scan History
SPTR37_9f  2  12   NSINGLE    
Initial Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
LLLVALLAFLSLGSGCHHR FSHR_BOVIN 3 3 -
LLLVSLLAFLSLGSGCHHR FSHR_HUMAN 3 3 -
LLLVSLLAFLSLGSGCHHR FSHR_MACFA 3 3 -
LLLVSLLAFLTLGSGCHHR FSHR_PIG 3 3 -
LLLVSLLAFLSLGSGCHHR FSHR_HORSE 3 3 -
LFLVALLAFLSLGSGCHHR FSHR_SHEEP 3 3 -
LLLVSLLAFLSLGSGCHHQ FSHR_EQUAS 3 3 -
LLLVSLLAFLGTGSGCHHW FSHR_RAT 3 3 -
LGLTCLLILLASCSGCQHH FSHR_CHICK 3 3 -

Motif 2 width=17
Element Seqn Id St Int Rpt
CSNGVFLCQESKVTEIP FSHR_BOVIN 25 3 -
CSNRVFLCQESKVTEIP FSHR_HUMAN 25 3 -
CSNRVFLCQESKVTEIP FSHR_MACFA 25 3 -
CSNGVFLCQESKVTEIP FSHR_PIG 25 3 -
CSNRVFLCQESKVTEIP FSHR_HORSE 25 3 -
CSNGVFLCQDSKVTEMP FSHR_SHEEP 25 3 -
YSNRVFLCQESKVTEIP FSHR_EQUAS 25 3 -
CSNRVFLCQDSKVTEIP FSHR_RAT 25 3 -
CEGRIFICQEIKVVQLP FSHR_CHICK 25 3 -

Motif 3 width=19
Element Seqn Id St Int Rpt
DLPRDAVELRFVLTKLRVI FSHR_BOVIN 43 1 -
DLPRNAIELRFVLTKLRVI FSHR_HUMAN 43 1 -
DLPRNAIELRFVHTKLRVI FSHR_MACFA 43 1 -
DLPRNAVELRFVLTKLRVI FSHR_PIG 43 1 -
DLPRNALELRFVLTKLRVI FSHR_HORSE 43 1 -
DLPRDAVELRFVLTKLRVI FSHR_SHEEP 43 1 -
DLPRNALELRFVLTKLRVI FSHR_EQUAS 43 1 -
DLPRNAIELRFVLTKLRVI FSHR_RAT 43 1 -
DIPTNATELRFVLTKMRVI FSHR_CHICK 43 1 -

Motif 4 width=12
Element Seqn Id St Int Rpt
GAFSGFGDLEKI FSHR_BOVIN 64 2 -
GAFSGFGDLEKI FSHR_HUMAN 64 2 -
GAFSGFGDLEKI FSHR_MACFA 64 2 -
GAFSGFGDLEKI FSHR_PIG 64 2 -
GAFSGFGDLEKI FSHR_HORSE 64 2 -
GAFSGFGDLEKI FSHR_SHEEP 64 2 -
GAFSGFGDLKKI FSHR_EQUAS 64 2 -
GSFAGFGDLEKI FSHR_RAT 64 2 -
GAFTGLHDLEKI FSHR_CHICK 64 2 -

Motif 5 width=21
Element Seqn Id St Int Rpt
LPKLHEIRIEKANNLLYIDPD FSHR_BOVIN 94 18 -
LPKLHEIRIEKANNLLYINPE FSHR_HUMAN 94 18 -
LPKLHEIRIEKANNLLYINPE FSHR_MACFA 94 18 -
LPKLHEIRIEKANNLLYIDPD FSHR_PIG 94 18 -
LPKLHEIRIEKANNLLYIDHD FSHR_HORSE 94 18 -
LPKLHEIRIEKANNLLYIDPD FSHR_SHEEP 94 18 -
LPKLHEIRIEKANNLLYIDHD FSHR_EQUAS 94 18 -
LPKLHEIRIEKANNLLYINPE FSHR_RAT 94 18 -
LPKLHEIRIEKANKLMKIDQD FSHR_CHICK 94 18 -

Motif 6 width=18
Element Seqn Id St Int Rpt
PAVHKIQSLQKVLLDIQD FSHR_BOVIN 136 21 -
PDVHKIHSLQKVLLDIQD FSHR_HUMAN 136 21 -
PDVHKIHSFQKVLLDIQD FSHR_MACFA 136 21 -
PAVHKIQSLQKVLLDIQD FSHR_PIG 136 21 -
PAVHKIQSLQKVLLDIQD FSHR_HORSE 136 21 -
PAVHKIQSLQKVLLDIQD FSHR_SHEEP 136 21 -
PAVHKIQSLQKVLLDIQD FSHR_EQUAS 136 21 -
PAVHKIQSLQKVLLDIQD FSHR_RAT 136 21 -
PVVHKVHSFQKVLLDVQD FSHR_CHICK 136 21 -

Motif 7 width=18
Element Seqn Id St Int Rpt
TQLDELNLSDNSNLEELP FSHR_BOVIN 193 39 -
TQLDELNLSDNNNLEELP FSHR_HUMAN 193 39 -
TQLDELNLSDNNNLEELP FSHR_MACFA 193 39 -
TQLDELNLSDNDNLEELP FSHR_PIG 193 39 -
TQLDELNLSYNNNLEELP FSHR_HORSE 193 39 -
TQLDELNLSDNSNLEELP FSHR_SHEEP 193 39 -
TQLDELNLSDNNNLEELP FSHR_EQUAS 193 39 -
TQLDELNLSDNNNLEELP FSHR_RAT 193 39 -
TCLDELNLSDNYNLEKLP FSHR_CHICK 193 39 -

Motif 8 width=21
Element Seqn Id St Int Rpt
NWRRQTSDLHPICNKSILRQE FSHR_BOVIN 280 69 -
NWRRQISELHPICNKSILRQE FSHR_HUMAN 280 69 -
NWRRQISELHPICNKSILRQE FSHR_MACFA 280 69 -
NWRRQISDLHPICNKSILRQE FSHR_PIG 280 69 -
NWRRQTSELQTTCNKSILRQE FSHR_HORSE 280 69 -
NWRRQTSDLHPICNKSILRQE FSHR_SHEEP 280 69 -
NWRQQTSELQTTCNKSILRQE FSHR_EQUAS 280 69 -
NLKRQISELHPICNKSILRQD FSHR_RAT 280 69 -
NRKTQNTEFYPICSMSPAKQD FSHR_CHICK 280 69 -

Motif 9 width=13
Element Seqn Id St Int Rpt
TQARGQRVSLAED FSHR_BOVIN 305 4 -
TQTRGQRSSLAED FSHR_HUMAN 305 4 -
TQTRGQRSSLAED FSHR_MACFA 305 4 -
TQARGQRVSLAED FSHR_PIG 305 4 -
TQARGERVSLAED FSHR_HORSE 304 3 -
TQARGQRISLAED FSHR_SHEEP 305 4 -
TQARGERVSLAED FSHR_EQUAS 304 3 -
TQIGDQRVSLIDD FSHR_RAT 305 4 -
TGKRKHRRSAAED FSHR_CHICK 305 4 -

Motif 10 width=18
Element Seqn Id St Int Rpt
RSETSSTAHNFHPRNGHC FSHR_BOVIN 655 337 -
RTETSSTVHNTHPRNGHC FSHR_HUMAN 655 337 -
RTETSSTAHNSHPRNGHC FSHR_MACFA 655 337 -
RTENLSTAHNIHPRNGHC FSHR_PIG 655 337 -
RTETSSTAHISHPRNGHC FSHR_HORSE 654 337 -
RSETSFTAHNFHPRNGHC FSHR_SHEEP 655 337 -
RTETSSTGHISHPKNGPC FSHR_EQUAS 647 330 -
RTETSSATHNFHARKSHC FSHR_RAT 654 336 -
RTETSSSAHNFHTRNGHY FSHR_CHICK 655 337 -
Final Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
LLLVALLAFLSLGSGCHHR FSHR_BOVIN 3 3 -
LLLVSLLAFLSLGSGCHHR FSHR_HUMAN 3 3 -
LLLVSLLAFLSLGSGCHHR FSHR_MACFA 3 3 -
LLLVSLLAFLTLGSGCHHR FSHR_PIG 3 3 -
LLLVSLLAFLSLGSGCHHR FSHR_HORSE 3 3 -
LFLVALLAFLSLGSGCHHR FSHR_SHEEP 3 3 -
LLLVSLLAFLSLGSGCHHQ FSHR_EQUAS 3 3 -
LLLVSLLAFLGTGSGCHHW FSHR_RAT 3 3 -
LLLVSLLAFLGTGSGCHHW Q64183 3 3 -
LGLTCLLILLASCSGCQHH FSHR_CHICK 3 3 -

Motif 2 width=17
Element Seqn Id St Int Rpt
CSNGVFLCQESKVTEIP FSHR_BOVIN 25 3 -
CSNRVFLCQESKVTEIP FSHR_HUMAN 25 3 -
CSNRVFLCQESKVTEIP FSHR_MACFA 25 3 -
CSNGVFLCQESKVTEIP FSHR_PIG 25 3 -
CSNRVFLCQESKVTEIP FSHR_HORSE 25 3 -
CSNGVFLCQDSKVTEMP FSHR_SHEEP 25 3 -
YSNRVFLCQESKVTEIP FSHR_EQUAS 25 3 -
CSNRVFLCQDSKVTEIP FSHR_RAT 25 3 -
CSNRVFLCQDSKVTEIP Q64183 25 3 -
CEGRIFICQEIKVVQLP FSHR_CHICK 25 3 -

Motif 3 width=19
Element Seqn Id St Int Rpt
DLPRDAVELRFVLTKLRVI FSHR_BOVIN 43 1 -
DLPRNAIELRFVLTKLRVI FSHR_HUMAN 43 1 -
DLPRNAIELRFVHTKLRVI FSHR_MACFA 43 1 -
DLPRNAVELRFVLTKLRVI FSHR_PIG 43 1 -
DLPRNALELRFVLTKLRVI FSHR_HORSE 43 1 -
DLPRDAVELRFVLTKLRVI FSHR_SHEEP 43 1 -
DLPRNALELRFVLTKLRVI FSHR_EQUAS 43 1 -
DLPRNAIELRFVLTKLRVI FSHR_RAT 43 1 -
DLPRNAIELRFVLTKLRVI Q64183 43 1 -
DIPTNATELRFVLTKMRVI FSHR_CHICK 43 1 -

Motif 4 width=12
Element Seqn Id St Int Rpt
GAFSGFGDLEKI FSHR_BOVIN 64 2 -
GAFSGFGDLEKI FSHR_HUMAN 64 2 -
GAFSGFGDLEKI FSHR_MACFA 64 2 -
GAFSGFGDLEKI FSHR_PIG 64 2 -
GAFSGFGDLEKI FSHR_HORSE 64 2 -
GAFSGFGDLEKI FSHR_SHEEP 64 2 -
GAFSGFGDLKKI FSHR_EQUAS 64 2 -
GSFAGFGDLEKI FSHR_RAT 64 2 -
GSFAGFGDLEKI Q64183 64 2 -
GAFTGLHDLEKI FSHR_CHICK 64 2 -

Motif 5 width=21
Element Seqn Id St Int Rpt
LPKLHEIRIEKANNLLYIDPD FSHR_BOVIN 94 18 -
LPKLHEIRIEKANNLLYINPE FSHR_HUMAN 94 18 -
LPKLHEIRIEKANNLLYINPE FSHR_MACFA 94 18 -
LPKLHEIRIEKANNLLYIDPD FSHR_PIG 94 18 -
LPKLHEIRIEKANNLLYIDHD FSHR_HORSE 94 18 -
LPKLHEIRIEKANNLLYIDPD FSHR_SHEEP 94 18 -
LPKLHEIRIEKANNLLYIDHD FSHR_EQUAS 94 18 -
LPKLHEIRIEKANNLLYINPE FSHR_RAT 94 18 -
LPKLHEIRIEKANNLLYINPE Q64183 94 18 -
LPKLHEIRIEKANKLMKIDQD FSHR_CHICK 94 18 -

Motif 6 width=18
Element Seqn Id St Int Rpt
PAVHKIQSLQKVLLDIQD FSHR_BOVIN 136 21 -
PDVHKIHSLQKVLLDIQD FSHR_HUMAN 136 21 -
PDVHKIHSFQKVLLDIQD FSHR_MACFA 136 21 -
PAVHKIQSLQKVLLDIQD FSHR_PIG 136 21 -
PAVHKIQSLQKVLLDIQD FSHR_HORSE 136 21 -
PAVHKIQSLQKVLLDIQD FSHR_SHEEP 136 21 -
PAVHKIQSLQKVLLDIQD FSHR_EQUAS 136 21 -
PAVHKIQSLQKVLLDIQD FSHR_RAT 136 21 -
PAVHKIQSLQKVLLDIQD Q64183 136 21 -
PVVHKVHSFQKVLLDVQD FSHR_CHICK 136 21 -

Motif 7 width=18
Element Seqn Id St Int Rpt
TQLDELNLSDNSNLEELP FSHR_BOVIN 193 39 -
TQLDELNLSDNNNLEELP FSHR_HUMAN 193 39 -
TQLDELNLSDNNNLEELP FSHR_MACFA 193 39 -
TQLDELNLSDNDNLEELP FSHR_PIG 193 39 -
TQLDELNLSYNNNLEELP FSHR_HORSE 193 39 -
TQLDELNLSDNSNLEELP FSHR_SHEEP 193 39 -
TQLDELNLSDNNNLEELP FSHR_EQUAS 193 39 -
TQLDELNLSDNNNLEELP FSHR_RAT 193 39 -
TQLDELNLSDNNNLEELP Q64183 189 35 -
TCLDELNLSDNYNLEKLP FSHR_CHICK 193 39 -

Motif 8 width=21
Element Seqn Id St Int Rpt
NWRRQTSDLHPICNKSILRQE FSHR_BOVIN 280 69 -
NWRRQISELHPICNKSILRQE FSHR_HUMAN 280 69 -
NWRRQISELHPICNKSILRQE FSHR_MACFA 280 69 -
NWRRQISDLHPICNKSILRQE FSHR_PIG 280 69 -
NWRRQTSELQTTCNKSILRQE FSHR_HORSE 280 69 -
NWRRQTSDLHPICNKSILRQE FSHR_SHEEP 280 69 -
NWRQQTSELQTTCNKSILRQE FSHR_EQUAS 280 69 -
NLKRQISELHPICNKSILRQD FSHR_RAT 280 69 -
NLKRQISELHPICNKSILRQD Q64183 276 69 -
NRKTQNTEFYPICSMSPAKQD FSHR_CHICK 280 69 -

Motif 9 width=13
Element Seqn Id St Int Rpt
TQARGQRVSLAED FSHR_BOVIN 305 4 -
TQTRGQRSSLAED FSHR_HUMAN 305 4 -
TQTRGQRSSLAED FSHR_MACFA 305 4 -
TQARGQRVSLAED FSHR_PIG 305 4 -
TQARGERVSLAED FSHR_HORSE 304 3 -
TQARGQRISLAED FSHR_SHEEP 305 4 -
TQARGERVSLAED FSHR_EQUAS 304 3 -
TQIGDQRVSLIDD FSHR_RAT 305 4 -
TQIGDQRVSLIDD Q64183 301 4 -
TGKRKHRRSAAED FSHR_CHICK 305 4 -

Motif 10 width=18
Element Seqn Id St Int Rpt
RSETSSTAHNFHPRNGHC FSHR_BOVIN 655 337 -
RTETSSTVHNTHPRNGHC FSHR_HUMAN 655 337 -
RTETSSTAHNSHPRNGHC FSHR_MACFA 655 337 -
RTENLSTAHNIHPRNGHC FSHR_PIG 655 337 -
RTETSSTAHISHPRNGHC FSHR_HORSE 654 337 -
RSETSFTAHNFHPRNGHC FSHR_SHEEP 655 337 -
RTETSSTGHISHPKNGPC FSHR_EQUAS 647 330 -
RTETSSATHNFHARKSHC FSHR_RAT 654 336 -
RTETSSATHNFHARKSHC Q64183 650 336 -
RTETSSSAHNFHTRNGHY FSHR_CHICK 655 337 -