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PR00889

Identifier
CALPONIN  [View Relations]  [View Alignment]  
Accession
PR00889
No. of Motifs
6
Creation Date
07-JUN-1998  (UPDATE 06-JUN-1999)
Title
Calponin signature
Database References
PRINTS; PR00888 SM22CALPONIN
INTERPRO; IPR001997
Literature References
1. STRASSER, P., GIMONA, M., MOESSLER, H., HERZOG, M. AND SMALL, J.V.
Mammalian calponin. Identification and expression of genetic variants.
FEBS LETT. 330 13-18 (1993). 
 
2. APPLEGATE, D., FENG, W., GREEN, R.S.and TAUBMAN, M.B.
Cloning and expression of a novel acidic calponin isoform from rat 
aortic vascular smooth muscle. 
J.BIOL.CHEM. 269 10683-10690 (1994). 
 
3. SAMAHA, F.F., IP, H.S., MORRISEY, E.E., SELTZER, J., TANG, Z., 
SOLWAY, J. AND PARMACEK, M.S.
Developmental pattern of expression and genomic organization of the 
calponin-h1 gene. a contractile smooth muscle cell marker. 
J.BIOL.CHEM. 271 395-403 (1996). 

Documentation
Calponin is a smooth muscle-specific, actin-, tropomyosin- and calmodulin-
binding protein believed to be involved in regulation or modulation of 
contraction [1,2]; interaction of the protein with actin inhibits actomyosin 
MgATPase activity. Multiple isoforms are found in smooth muscle [2].
 
Calponin is a basic protein of ~34 Kd [3]. The protein contains three 
repeats of a well-conserved 26-residue domain, which is also found in a 
variety of proteins whose physiological role is as yet unclear (these 
include vertebrate smooth muscle protein, Drosophila synchronous flight 
muscle protein, C.elegans unc-87 protein, and rat neuronal protein NP25).
 
CALPONIN is a 6-element fingerprint that provides a signature for the
calponins. The fingerprint was derived from an initial alignment of 6
sequences: the motifs were drawn from short conserved sections, focusing
on those regions that characterise the calponins but distinguish them from
the rest of the SM22/calponin family - motif 4 includes the C-terminus of
the first calponin domain repeat; motif 5 lies within the second domain
repeat and includes the central part of the region encoded by PROSITE 
pattern CALPONIN (PS01052); and motif 6 includes the third repeat. Two 
iterations on OWL30.2 were required to reach convergence, at which point
a true set comprising 12 sequences was identified. Several partial matches
were also found, all of which are fragments and/or SM22/calponin family
members that fail to match one or more motifs.
 
An update on SPTR37_9f identified a true set of 10 sequences.
Summary Information
10 codes involving  6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
6101010101010
5000000
4000000
3000000
2000000
123456
True Positives
CLP1_HUMAN    CLP1_MOUSE    CLP1_PIG      CLP1_RAT      
CLP2_MOUSE CLPA_RAT CLPO_CHICK O93547
Q15417 Q92578
Sequence Titles
CLP1_HUMAN  CALPONIN H1, SMOOTH MUSCLE (BASIC CALPONIN) - HOMO SAPIENS (HUMAN). 
CLP1_MOUSE CALPONIN H1, SMOOTH MUSCLE (BASIC CALPONIN) - MUS MUSCULUS (MOUSE).
CLP1_PIG CALPONIN H1, SMOOTH MUSCLE (BASIC CALPONIN) - SUS SCROFA (PIG).
CLP1_RAT CALPONIN H1, SMOOTH MUSCLE (BASIC CALPONIN) - RATTUS NORVEGICUS (RAT).
CLP2_MOUSE CALPONIN H2, SMOOTH MUSCLE - MUS MUSCULUS (MOUSE).
CLPA_RAT CALPONIN, ACIDIC ISOFORM (CALPONIN, NON-MUSCLE ISOFORM) - RATTUS NORVEGICUS (RAT).
CLPO_CHICK CALPONIN ALPHA AND BETA, SMOOTH MUSCLE - GALLUS GALLUS (CHICKEN).
O93547 CALPONIN H3 - XENOPUS LAEVIS (AFRICAN CLAWED FROG).
Q15417 ACIDIC CALPONIN - HOMO SAPIENS (HUMAN).
Q92578 NEUTRAL CALPONIN - HOMO SAPIENS (HUMAN).
Scan History
OWL30_2    2  100  NSINGLE    
SPTR37_9f 2 12 NSINGLE
Initial Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
QELREWIEGVTGRRI CLP1_PIG 32 32 -
QELREWIEGVTGRRI CLP1_RAT 32 32 -
QELREWIEGVTGRRI CLP1_MOUSE 32 32 -
QELREWIEGVTGRRI CLP1_HUMAN 32 32 -
EDLRNWIEEVTGMGI CLPA_RAT 30 30 -
AELRSWIEGLTGLSI CLP2_PIG 32 32 -

Motif 2 width=18
Element Seqn Id St Int Rpt
CEFINKLQPGSVKKVNES CLP1_PIG 61 14 -
CEFINKLQPGSVKKVNES CLP1_MOUSE 61 14 -
CTLMNKLQPGSVPKINRS CLP2_PIG 61 14 -
CELINKLQPGSVKKVNES CLPA_RAT 59 14 -
CEFINKLQPGSVKKINES CLP1_HUMAN 61 14 -
CEFINKLQPGSVKKVNES CLP1_RAT 61 14 -

Motif 3 width=16
Element Seqn Id St Int Rpt
ENTNHTQVQSTLLALA CLP1_MOUSE 113 34 -
ESGNMTQVQVSLLALA CLP2_PIG 113 34 -
ENTNHTQVQSTLLALA CLP1_PIG 113 34 -
ENTNHTQVQSTLLALA CLP1_RAT 113 34 -
ENTNHTQVQSTLLALA CLP1_HUMAN 113 34 -
ENGNMTQVQTTLVALA CLPA_RAT 111 34 -

Motif 4 width=20
Element Seqn Id St Int Rpt
YGTRRHLYDPKLGTDQPLDQ CLP1_HUMAN 182 53 -
YGTRRHLYDPKNHILPPMDH CLP2_PIG 184 55 -
YGTRRHLYDPKMQTDKPFDQ CLPA_RAT 182 55 -
YGTRRHLYDPKLGTDQPLDQ CLP1_PIG 182 53 -
YGTRRHLYDPKLGTDQPLDQ CLP1_RAT 182 53 -
YGTRRHLYDPKLGTDQPLDQ CLP1_MOUSE 182 53 -

Motif 5 width=16
Element Seqn Id St Int Rpt
QATISLQMGTNKGASQ CLP1_RAT 201 -1 -
QATISLQMGTNKGASQ CLP1_PIG 201 -1 -
QATISLQMGTNKGASQ CLP1_MOUSE 201 -1 -
QATISLQMGTNKGASQ CLP1_HUMAN 201 -1 -
QTTISLQMGTNKGASQ CLPA_RAT 201 -1 -
HSTISLQMGTNKCASQ CLP2_PIG 203 -1 -

Motif 6 width=18
Element Seqn Id St Int Rpt
TVYGLPRQVYDPKYCLTP CLP1_RAT 259 42 -
SVYGLGRQVYDPKYCAAP CLPA_RAT 259 42 -
TVYGLPRQVYDPKYCLTP CLP1_PIG 259 42 -
TVYGLPRQVYDPKYCLTP CLP1_HUMAN 259 42 -
QVFGLGRQIYDPKYCPQG CLP2_PIG 260 41 -
TVYGLPRQVYDPKYCLNP CLP1_MOUSE 259 42 -
Final Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
QELREWIEGVTGRRI CLP1_PIG 32 32 -
QELREWIEGVTGRRI CLP1_RAT 32 32 -
QELREWIEGVTGRRI CLP1_MOUSE 32 32 -
QELREWIEGVTGRRI CLP1_HUMAN 32 32 -
EDLRNWIEEVTGMSI Q15417 30 30 -
EDLRNWIEEVTGMGI CLPA_RAT 30 30 -
RQLRVWIEGATGRRI CLPO_CHICK 32 32 -
AELRTWIEGLTGLSI Q92578 32 32 -
AELRSWIEGLTGLSI CLP2_MOUSE 32 32 -
TELKVWIEEVTGMSI O93547 31 31 -

Motif 2 width=18
Element Seqn Id St Int Rpt
CEFINKLQPGSVKKVNES CLP1_PIG 61 14 -
CEFINKLQPGSVKKVNES CLP1_RAT 61 14 -
CEFINKLQPGSVKKVNES CLP1_MOUSE 61 14 -
CEFINKLQPGSVKKINES CLP1_HUMAN 61 14 -
CELINKLQPGSVKKVNES Q15417 59 14 -
CELINKLQPGSVKKVNES CLPA_RAT 59 14 -
CELINTLQPGSVQKVNDP CLPO_CHICK 61 14 -
CTLMNKLQPGSVPKINRS Q92578 61 14 -
CTLMNKLQPGSVPKINRS CLP2_MOUSE 61 14 -
CELMNKLRPRAIPKVNVS O93547 60 14 -

Motif 3 width=16
Element Seqn Id St Int Rpt
ENTNHTQVQSTLLALA CLP1_PIG 113 34 -
ENTNHTQVQSTLLALA CLP1_RAT 113 34 -
ENTNHTQVQSTLLALA CLP1_MOUSE 113 34 -
ENTNHTQVQSTLLALA CLP1_HUMAN 113 34 -
ENGNMTQVQTTLVALA Q15417 111 34 -
ENGNMTQVQTTLVALA CLPA_RAT 111 34 -
ENTNHTQVQSTLIALA CLPO_CHICK 113 34 -
ESGNMTQVQVSLLALA Q92578 113 34 -
ESGNMTQVQVSLLALA CLP2_MOUSE 113 34 -
ENGNMTQVQVSLLSLA O93547 112 34 -

Motif 4 width=20
Element Seqn Id St Int Rpt
YGTRRHLYDPKLGTDQPLDQ CLP1_PIG 182 53 -
YGTRRHLYDPKLGTDQPLDQ CLP1_RAT 182 53 -
YGTRRHLYDPKLGTDQPLDQ CLP1_MOUSE 182 53 -
YGTRRHLYDPKLGTDQPLDQ CLP1_HUMAN 182 53 -
YGTRRHLYDPKMQTDKPFDQ Q15417 182 55 -
YGTRRHLYDPKMQTDKPFDQ CLPA_RAT 182 55 -
YGTRRHLYDPKLGTDQPLDQ CLPO_CHICK 182 53 -
YGTRRHLYDPKNHILPPMDH Q92578 184 55 -
YGTRRHLYDPKNHILPPMDH CLP2_MOUSE 184 55 -
YGTRRHLYDPKNTILPPMDH O93547 183 55 -

Motif 5 width=16
Element Seqn Id St Int Rpt
QATISLQMGTNKGASQ CLP1_PIG 201 -1 -
QATISLQMGTNKGASQ CLP1_RAT 201 -1 -
QATISLQMGTNKGASQ CLP1_MOUSE 201 -1 -
QATISLQMGTNKGASQ CLP1_HUMAN 201 -1 -
QTTISLQMGTNKGASQ Q15417 201 -1 -
QTTISLQMGTNKGASQ CLPA_RAT 201 -1 -
QATISLQMGTNKGASQ CLPO_CHICK 201 -1 -
HSTISLQMGTNKCASQ Q92578 203 -1 -
HCTISLQMGTNKCASQ CLP2_MOUSE 203 -1 -
HSTISLQMGSNKGASQ O93547 202 -1 -

Motif 6 width=18
Element Seqn Id St Int Rpt
TVYGLPRQVYDPKYCLTP CLP1_PIG 259 42 -
TVYGLPRQVYDPKYCLTP CLP1_RAT 259 42 -
TVYGLPRQVYDPKYCLNP CLP1_MOUSE 259 42 -
TVYGLPRQVYDPKYCLTP CLP1_HUMAN 259 42 -
SVYGLGRQVYDPKYCAAP Q15417 259 42 -
SVYGLGRQVYDPKYCAAP CLPA_RAT 259 42 -
TVYGLPRQVYDPKYCDAP CLPO_CHICK 259 42 -
QVFGLGRQIYDPKYCPQG Q92578 260 41 -
QVFGLGRQIYDPKYCPQG CLP2_MOUSE 260 41 -
QIFGLGRQIYDPKYCPTG O93547 259 41 -