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PR00748

Identifier
MELIBIASE  [View Relations]  [View Alignment]  
Accession
PR00748
No. of Motifs
8
Creation Date
11-JUN-1997  (UPDATE 07-JUN-1999)
Title
Melibiase signature
Database References

PROSITE; PS00512 ALPHA_GALACTOSIDASE
BLOCKS; BL00512
INTERPRO; IPR002287
Literature References
1. HENRISSAT, B. AND BAIROCH, A.
New families in the classification of glycosyl hydrolases based on amino
acid sequence similarities.
BIOCHEM.J. 293 781-788 (1993).
 
2. HENRISSAT, B.
A classification of glycosyl hydrolases based on amino acid sequence
similarities.
BIOCHEM.J. 280 309-316 (1991).
 
3. DAVIES, G. AND HENRISSAT, B.
Structures and mechanisms of glycosyl hydrolases.
STRUCTURE 3 853-859 (1995).
 
4. HENRISSAT, B. AND BAIROCH, A.
Updating the sequence-based classification of glycosyl hydrolases.
BIOCHEM.J. 316 695-696 (1996).
 
5. TURAKAINEN, H., KRISTO, P. AND KORHOLA, M. 
Consideration of the evolution of the Saccharomyces cerevisiae MEL gene
family on the basis of the nucleotide sequences of the genes and their
flanking regions.
YEAST 10(12) 1559-1568 (1994). 

Documentation
O-Glycosyl hydrolases (EC 3.2.1.-) are a widespread group of enzymes that
hydrolyse the glycosidic bond between two or more carbohydrates, or between
a carbohydrate and a non-carbohydrate moiety. A classification system for
glycosyl hydrolases, based on sequence similarity, has led to the definition
of up to 60 different families [1-4] (http://expasy.hcuge.ch/cgi-bin/lists?
glycosid.txt). Family 27 encompasses alpha-galactosidases (melibiase) and
alpha-N-acetylgalactosaminidases.
 
Melibiase catalyses the hydrolysis of melibiose into galactose and glucose
[5]. In man, deficiency in the enzyme results in Fabry's disease (X-linked
sphingolipidosis).  
 
MELIBIASE is an 8-element fingerprint that provides a signature for
melibiases. The fingerprint was derived from an initial alignment of 4
sequences: the motifs were drawn from conserved regions spanning the
central and C-terminal portions of the alignment. Two iterations on OWL29.3
were required to reach convergence, at which point a true set comprising 8
sequences was identified. A single partial match was also found, S79400, a
related sequence that matches only motifs 1 and 4.
 
An update on SPTR37_9f identified a true set of 8 sequences.
Summary Information
8 codes involving  8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
888888888
700000000
600000000
500000000
400000000
300000000
200000000
12345678
True Positives
MEL1_YEAST    MEL2_YEAST    MEL5_YEAST    MEL6_YEAST    
Q03647 Q09187 Q11129 Q99172
Sequence Titles
MEL1_YEAST  ALPHA-GALACTOSIDASE 1 PRECURSOR (EC 3.2.1.22) (MELIBIASE 1) (ALPHA-D- GALACTOSIDE GALACTOHYDROLASE 1) - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST). 
MEL2_YEAST ALPHA-GALACTOSIDASE 2 PRECURSOR (EC 3.2.1.22) (MELIBIASE 2) (ALPHA-D- GALACTOSIDE GALACTOHYDROLASE 2) - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
MEL5_YEAST ALPHA-GALACTOSIDASE 5 PRECURSOR (EC 3.2.1.22) (MELIBIASE 5) (ALPHA-D- GALACTOSIDE GALACTOHYDROLASE 5) - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
MEL6_YEAST ALPHA-GALACTOSIDASE 6 PRECURSOR (EC 3.2.1.22) (MELIBIASE 6) (ALPHA-D- GALACTOSIDE GALACTOHYDROLASE 6) - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
Q03647 ALPHA-GALACTOSIDASE PRECURSOR (EC 3.2.1.22) (MELIBIASE) (ALPHA-D-GALACTOSIDE GALACTOHYDROLASE) - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
Q09187 ALPHA-GALACTOSIDASE MEL GENE - SACCHAROMYCES PARADOXUS (YEAST).
Q11129 ALPHA-GALACTOSIDASE MEL GENE - SACCHAROMYCES SP.
Q99172 ALPHA-GALACTOSIDASE - ZYGOSACCHAROMYCES CIDRI.
Scan History
OWL29_3    2  100  NSINGLE    
SPTR37_9f 2 9 NSINGLE
Initial Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
NCYNKGQFGTPEISYH MEL2_YEAST 150 150 -
NCYNKGQFGTPDVSYH MEL5_YEAST 150 150 -
NCYNKGQFGTPDVSYH MEL6_YEAST 150 150 -
NCYNKGQFGTPEISYH MEL1_YEAST 150 150 -

Motif 2 width=20
Element Seqn Id St Int Rpt
EFTRPDSRCPCDGDEYDCKY MEL2_YEAST 213 47 -
EFTRPDSRCPCDGDEYDCKY MEL5_YEAST 213 47 -
EFTRPDSRCPCDGDEYDCKY MEL6_YEAST 213 47 -
EFTRPDSRCPCDGDEYDCKY MEL1_YEAST 213 47 -

Motif 3 width=17
Element Seqn Id St Int Rpt
RVWRYYVSDTDEYGQGE MEL2_YEAST 326 93 -
RVWRYYVSDTDEYGQGE MEL5_YEAST 326 93 -
RVWRYYVSDTDEYGQGE MEL6_YEAST 326 93 -
RVWRYYVSDTDEYGQGE MEL1_YEAST 326 93 -

Motif 4 width=17
Element Seqn Id St Int Rpt
DNGDQVVALLNGGSVPR MEL2_YEAST 351 8 -
DNGDQVVALLNGGSVAR MEL5_YEAST 351 8 -
DNGDQVVALLNGGSVAR MEL6_YEAST 351 8 -
DNGDQVVALLNGGSVSR MEL1_YEAST 351 8 -

Motif 5 width=24
Element Seqn Id St Int Rpt
EEIFFDSNLGSKELTSTWDIYDLW MEL2_YEAST 374 6 -
EEIFFDSNLGSKELTSTWDIYDLW MEL5_YEAST 374 6 -
EEIFFDSNLGSKELTSTWDIYDLW MEL6_YEAST 374 6 -
EEIFFDSNLGSKKLTSTWDIYDLW MEL1_YEAST 374 6 -

Motif 6 width=23
Element Seqn Id St Int Rpt
RVDNSTASAILGQNKTATGILYN MEL2_YEAST 400 2 -
RVDNSTASAILEQNKAATGILYN MEL5_YEAST 400 2 -
RVDNSTASAILEQNKAATGILYN MEL6_YEAST 400 2 -
RVDNSTASAILGRNKTATGILYN MEL1_YEAST 400 2 -

Motif 7 width=17
Element Seqn Id St Int Rpt
YKDGLSKNDTRLFGQKI MEL2_YEAST 428 5 -
YKDGLSKNDTRLFGQKI MEL5_YEAST 428 5 -
YKDGLSKNDTRLFGQKI MEL6_YEAST 428 5 -
YKDGLSKNDTRLFGQKI MEL1_YEAST 428 5 -

Motif 8 width=21
Element Seqn Id St Int Rpt
PNAILNTTVPAHGIAFYRLRP MEL2_YEAST 449 4 -
PNAILNTTVPAHGIAFYRLRP MEL5_YEAST 449 4 -
PNAILNTTVPAHGIAFYRLRP MEL6_YEAST 449 4 -
PNAILNTTVPAHGIAFYRLRP MEL1_YEAST 449 4 -
Final Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
NCYNKGQFGTPEISYH MEL2_YEAST 150 150 -
NCYNKGQFGTPDVSYH MEL5_YEAST 150 150 -
NCYNKGQFGTPDVSYH MEL6_YEAST 150 150 -
NCYNKGQFGTPEISYH MEL1_YEAST 150 150 -
NCYNKGKFGTPETSYK Q11129 150 150 -
NCYNKGRFGTPESSHK Q09187 150 150 -
NCYNKGQFGAPETSYK Q03647 150 150 -
NCYNKGEFGTPEISYK Q99172 149 149 -

Motif 2 width=20
Element Seqn Id St Int Rpt
EFTRPDSRCPCDGDEYDCKY MEL2_YEAST 213 47 -
EFTRPDSRCPCDGDEYDCKY MEL5_YEAST 213 47 -
EFTRPDSRCPCDGDEYDCKY MEL6_YEAST 213 47 -
EFTRPDSRCPCDGDEYDCKY MEL1_YEAST 213 47 -
EFDRPDSRCPCDGDQYDCSY Q11129 213 47 -
EFSRPDSRCPCDGDEYDCKY Q09187 213 47 -
QFTRPDSRCPCDGDQFDCAY Q03647 213 47 -
QFDRPDSRCPCSGDEYDCSY Q99172 212 47 -

Motif 3 width=17
Element Seqn Id St Int Rpt
RVWRYYVSDTDEYGQGE MEL2_YEAST 326 93 -
RVWRYYVSDTDEYGQGE MEL5_YEAST 326 93 -
RVWRYYVSDTDEYGQGE MEL6_YEAST 326 93 -
RVWRYYVSDTDEYGQGE MEL1_YEAST 326 93 -
RVWRHYVSQTDKYGKGE Q11129 326 93 -
RVWRYYVPQTDKYGQGE Q09187 326 93 -
RVWRHQVPQTDKYGQGE Q03647 326 93 -
RIWKYHVSDKDQYGEGE Q99172 325 93 -

Motif 4 width=17
Element Seqn Id St Int Rpt
DNGDQVVALLNGGSVPR MEL2_YEAST 351 8 -
DNGDQVVALLNGGSVAR MEL5_YEAST 351 8 -
DNGDQVVALLNGGSVAR MEL6_YEAST 351 8 -
DNGDQVVALLNGGSVSR MEL1_YEAST 351 8 -
DNGDQVIALLNGSNKKR Q11129 351 8 -
ENGDQVIALLNGGMKAR Q09187 351 8 -
DNGDQVIALLNGGIKPR Q03647 351 8 -
DNGDHVVALLNGGNNER Q99172 350 8 -

Motif 5 width=24
Element Seqn Id St Int Rpt
EEIFFDSNLGSKELTSTWDIYDLW MEL2_YEAST 374 6 -
EEIFFDSNLGSKELTSTWDIYDLW MEL5_YEAST 374 6 -
EEIFFDSNLGSKELTSTWDIYDLW MEL6_YEAST 374 6 -
EEIFFDSNLGSKKLTSTWDIYDLW MEL1_YEAST 374 6 -
EDIFFDSYLGSEELSSSWDIYDLW Q11129 374 6 -
EDIFFDSYQGSEELSTSWDIYDLW Q09187 374 6 -
EEIFFDSYLGFEQLSSNWDIYDLW Q03647 374 6 -
NDIFIDYLADSDELSNTWGLYDLW Q99172 373 6 -

Motif 6 width=23
Element Seqn Id St Int Rpt
RVDNSTASAILGQNKTATGILYN MEL2_YEAST 400 2 -
RVDNSTASAILEQNKAATGILYN MEL5_YEAST 400 2 -
RVDNSTASAILEQNKAATGILYN MEL6_YEAST 400 2 -
RVDNSTASAILGRNKTATGILYN MEL1_YEAST 400 2 -
RIDNTIASNILKNNKVTNSSLYN Q11129 400 2 -
RIDNATASAILENNKVTNNTLYN Q09187 400 2 -
RVDNATSANILNNNSVGNATIYN Q03647 400 2 -
RMSNATAASILSGNMTSAGLNYN Q99172 399 2 -

Motif 7 width=17
Element Seqn Id St Int Rpt
YKDGLSKNDTRLFGQKI MEL2_YEAST 428 5 -
YKDGLSKNDTRLFGQKI MEL5_YEAST 428 5 -
YKDGLSKNDTRLFGQKI MEL6_YEAST 428 5 -
YKDGLSKNDTRLFGQKI MEL1_YEAST 428 5 -
YKEGLSKNDTRLFGVQI Q11129 428 5 -
YKEGLLNGDSRLFGTKV Q09187 428 5 -
YKDGMAKNDTRLFGTKI Q03647 428 5 -
YTAGIARNDSRLFGDRV Q99172 427 5 -

Motif 8 width=21
Element Seqn Id St Int Rpt
PNAILNTTVPAHGIAFYRLRP MEL2_YEAST 449 4 -
PNAILNTTVPAHGIAFYRLRP MEL5_YEAST 449 4 -
PNAILNTTVPAHGIAFYRLRP MEL6_YEAST 449 4 -
PNAILNTTVPAHGIAFYRLRP MEL1_YEAST 449 4 -
PGGLLNTTVPAHGVALYRLRR Q11129 449 4 -
PDGIINTTVPAHGIALYRLRS Q09187 449 4 -
PDGLLNTTVPAHGIAFYRLRR Q03647 449 4 -
KGESLTATVPGHGVALFRLRP Q99172 448 4 -