SPRINT Home UMBER Home Contents Standard Search Advanced Search Relation Search

==SPRINT==> PRINTS View



  selected as


PR00745

Identifier
GLHYDRLASE39  [View Relations]  [View Alignment]  
Accession
PR00745
No. of Motifs
7
Creation Date
11-JUN-1997  (UPDATE 07-JUN-1999)
Title
Glycosyl hydrolase family 39 signature
Database References

PROSITE; PS01027 GLYCOSYL_HYDROL_F39
BLOCKS; BL01027
PFAM; PF01229 Glycosyl_hydr19
INTERPRO; IPR000514
Literature References
1. HENRISSAT, B. AND BAIROCH, A.
New families in the classification of glycosyl hydrolases based on amino
acid sequence similarities.
BIOCHEM.J. 293 781-788 (1993).
 
2. HENRISSAT, B.
A classification of glycosyl hydrolases based on amino acid sequence
similarities.
BIOCHEM.J. 280 309-316 (1991).
 
3. DAVIES, G. AND HENRISSAT, B.
Structures and mechanisms of glycosyl hydrolases.
STRUCTURE 3 853-859 (1995).
 
4. HENRISSAT, B. AND BAIROCH, A.
Updating the sequence-based classification of glycosyl hydrolases.
BIOCHEM.J. 316 695-696 (1996).
 
5. STOLTZFUS, L.J., SOSA-PINEDA, B., MOSKOWITZ, S.M., MENON, K.P.,
DLOTT, B., HOOPER, L., TEPLOW, D.B., SHULL, R.M. AND NEUFELD, E.F.
Cloning and characterization of cDNA encoding canine alpha-L-iduronidase.
mRNA deficiency in mucopolysaccharidosis I dog.
J.BIOL.CHEM. 267(10) 6570-6575 (1992). 

Documentation
O-Glycosyl hydrolases (EC 3.2.1.-) are a widespread group of enzymes that
hydrolyse the glycosidic bond between two or more carbohydrates, or between
a carbohydrate and a non-carbohydrate moiety. A classification system for
glycosyl hydrolases, based on sequence similarity, has led to the definition
of up to 60 different families [1-4] (http://expasy.hcuge.ch/cgi-bin/lists?
glycosid.txt). Family 39 encompasses mammalian alpha-L-iduronidases and some
bacterial beta-xylosidases.
 
Alpha-L-iduronidase is a lysosomal enzyme, deficiency of which causes muco-
polysaccharidosis I (MPS I) [5]. The enzyme hydrolyses alpha-L-iduronosidic
linkages in desulphated dermatan.
 
GLHYDRLASE39 is a 7-element fingerprint that provides a signature for
family 39 glycosyl hydrolases. The fingerprint was derived from an initial
alignment of 5 sequences: the motifs were drawn from conserved regions 
spanning the central portion of the alignment - motif 3 includes the
region encoded by PROSITE pattern GLYCOSYL_HYDROL_F39 (PS01027), which
contains a Glu residue that may act as the proton donor in the catalytic
mechanism. A single iteration on OWL29.3 was required to reach convergence,
no further sequences being identified beyond the starting set.
 
An update on SPTR37_9f identified a true set of 6 sequences, and 1
partial match.
Summary Information
   6 codes involving  7 elements
1 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
76666666
61111110
50000000
40000000
30000000
20000000
1234567
True Positives
IDUA_CANFA    IDUA_HUMAN    IDUA_MOUSE    O30360        
XYNB_CALSA XYNB_THESA
True Positive Partials
Codes involving 6 elements
O30428
Sequence Titles
IDUA_CANFA  ALPHA-L-IDURONIDASE PRECURSOR (EC 3.2.1.76) - CANIS FAMILIARIS (DOG). 
IDUA_HUMAN ALPHA-L-IDURONIDASE PRECURSOR (EC 3.2.1.76) - HOMO SAPIENS (HUMAN).
IDUA_MOUSE ALPHA-L-IDURONIDASE PRECURSOR (EC 3.2.1.76) - MUS MUSCULUS (MOUSE).
O30360 BETA-XYLOSIDASE - THERMOANAEROBACTERIUM SP. 'JW/SL YS485'.
XYNB_CALSA BETA-XYLOSIDASE (EC 3.2.1.37) (1,4-BETA-D-XYLAN XYLOHYDROLASE) (XYLAN 1,4-BETA-XYLOSIDASE) - CALDOCELLUM SACCHAROLYTICUM (CALDICELLULOSIRUPTOR SACCHAROLYTICUS).
XYNB_THESA BETA-XYLOSIDASE (EC 3.2.1.37) (1,4-BETA-D-XYLAN XYLOHYDROLASE) (XYLAN 1,4-BETA-XYLOSIDASE) - THERMOANAEROBACTER SACCHAROLYTICUM.

O30428 XYLANASE - CALDOCELLUM SACCHAROLYTICUM (CALDICELLULOSIRUPTOR SACCHAROLYTICUS).
Scan History
OWL29_3    1  25   NSINGLE    
SPTR37_9f 2 7 NSINGLE
Initial Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
LDGYLDLLRENQLLPGF IDUA_HUMAN 114 114 -
LDGYLDLLRENQLLPGF IDUA_CANFA 113 113 -
LDAFLDLLMENQLLPGF IDUA_MOUSE 104 104 -
FDSFLEIGIRPFVEIGF XYNB_THESA 86 86 -
IDFLLEIGMKPFIELSF XYNB_CALSA 89 89 -

Motif 2 width=18
Element Seqn Id St Int Rpt
LVSSLARRYIGRYGLAHV IDUA_HUMAN 155 24 -
LVSLLARRYIGRYGLSYV IDUA_CANFA 154 24 -
LVSLLARRYIGRYGLTHV IDUA_MOUSE 145 24 -
LVKAVLHHFISRYGIEEV XYNB_THESA 133 30 -
LIEELARHLISRYGKNEV XYNB_CALSA 136 30 -

Motif 3 width=17
Element Seqn Id St Int Rpt
VSKWNFETWNEPDHHDF IDUA_HUMAN 172 -1 -
VSKWNFETWNEPDHHDF IDUA_CANFA 171 -1 -
VSKWNFETWNEPDHHDF IDUA_MOUSE 162 -1 -
VLKWPFEIWNEPNLKEF XYNB_THESA 150 -1 -
VREWFFEVWNEPNLKDF XYNB_CALSA 153 -1 -

Motif 4 width=15
Element Seqn Id St Int Rpt
FTGEAGVRLDYISLH IDUA_HUMAN 248 59 -
FTGELGVRLDYISLH IDUA_CANFA 247 59 -
FTGEVGVRLDYISLH IDUA_MOUSE 238 59 -
FCYEENVPVDFVSRH XYNB_THESA 214 47 -
FCTKNGVPIDFISTH XYNB_CALSA 215 45 -

Motif 5 width=18
Element Seqn Id St Int Rpt
PWRADVTYAAMVVKVIAQ IDUA_HUMAN 311 48 -
PWRADVTYAAMVVKVVAQ IDUA_CANFA 310 48 -
PWRADVTYAALVVKVIAQ IDUA_MOUSE 301 48 -
PVHDTPFNAAYIARILSE XYNB_THESA 287 58 -
PYHDIPYDAAFIVKTIID XYNB_CALSA 285 55 -

Motif 6 width=12
Element Seqn Id St Int Rpt
LDNGLCSPDGEW IDUA_HUMAN 476 147 -
LDNPRCSPHGEW IDUA_CANFA 475 147 -
LDNQLSSPYSAW IDUA_MOUSE 466 147 -
IDEEHGNPWGTW XYNB_THESA 415 110 -
IDEYNANPKRVW XYNB_CALSA 420 117 -

Motif 7 width=15
Element Seqn Id St Int Rpt
WRRLGRPVFPTAEQF IDUA_HUMAN 487 -1 -
WQRLGRPVFPTAEEF IDUA_CANFA 486 -1 -
WQHMGQPVFPSAEQF IDUA_MOUSE 477 -1 -
WIHMGRPRYPSKEQV XYNB_THESA 426 -1 -
WLEMGSPAYLNREQI XYNB_CALSA 431 -1 -
Final Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
LDGYLDLLRENQLLPGF IDUA_HUMAN 114 114 -
LDGYLDLLRENQLLPGF IDUA_CANFA 113 113 -
LDAFLDLLMENQLLPGF IDUA_MOUSE 104 104 -
FDSFLEIGIRPFVEIGF XYNB_THESA 86 86 -
FDSFLEIGIRPFVEIGF O30360 86 86 -
IDFLLEIGMKPFIELSF XYNB_CALSA 89 89 -

Motif 2 width=18
Element Seqn Id St Int Rpt
LVSSLARRYIGRYGLAHV IDUA_HUMAN 155 24 -
LVSLLARRYIGRYGLSYV IDUA_CANFA 154 24 -
LVSLLARRYIGRYGLTHV IDUA_MOUSE 145 24 -
LVKAVLHHFISRYGIEEV XYNB_THESA 133 30 -
LIKAVVSHFISRYGIDEV O30360 133 30 -
LIEELARHLISRYGKNEV XYNB_CALSA 136 30 -

Motif 3 width=17
Element Seqn Id St Int Rpt
VSKWNFETWNEPDHHDF IDUA_HUMAN 172 -1 -
VSKWNFETWNEPDHHDF IDUA_CANFA 171 -1 -
VSKWNFETWNEPDHHDF IDUA_MOUSE 162 -1 -
VLKWPFEIWNEPNLKEF XYNB_THESA 150 -1 -
VAKWPFEIWNEPNLKEF O30360 150 -1 -
VREWFFEVWNEPNLKDF XYNB_CALSA 153 -1 -

Motif 4 width=15
Element Seqn Id St Int Rpt
FTGEAGVRLDYISLH IDUA_HUMAN 248 59 -
FTGELGVRLDYISLH IDUA_CANFA 247 59 -
FTGEVGVRLDYISLH IDUA_MOUSE 238 59 -
FCYEENVPVDFVSRH XYNB_THESA 214 47 -
FCYEENVPVDFVSRH O30360 214 47 -
FCTKNGVPIDFISTH XYNB_CALSA 215 45 -

Motif 5 width=18
Element Seqn Id St Int Rpt
PWRADVTYAAMVVKVIAQ IDUA_HUMAN 311 48 -
PWRADVTYAAMVVKVVAQ IDUA_CANFA 310 48 -
PWRADVTYAALVVKVIAQ IDUA_MOUSE 301 48 -
PVHDTPFNAAYIARILSE XYNB_THESA 287 58 -
PVHDTPFNAAYIARILSE O30360 287 58 -
PYHDIPYDAAFIVKTIID XYNB_CALSA 285 55 -

Motif 6 width=12
Element Seqn Id St Int Rpt
LDNGLCSPDGEW IDUA_HUMAN 476 147 -
LDNPRCSPHGEW IDUA_CANFA 475 147 -
LDNQLSSPYSAW IDUA_MOUSE 466 147 -
IDEEHGNPWGTW XYNB_THESA 415 110 -
IDEEHGNPWGTW O30360 415 110 -
IDEYNANPKRVW XYNB_CALSA 420 117 -

Motif 7 width=15
Element Seqn Id St Int Rpt
WRRLGRPVFPTAEQF IDUA_HUMAN 487 -1 -
WQRLGRPVFPTAEEF IDUA_CANFA 486 -1 -
WQHMGQPVFPSAEQF IDUA_MOUSE 477 -1 -
WIHMGRPRYPSKEQV XYNB_THESA 426 -1 -
WIHMGRPRYPSKKEI O30360 426 -1 -
WLEMGSPAYLNREQI XYNB_CALSA 431 -1 -