SPRINT Home UMBER Home Contents Standard Search Advanced Search Relation Search

==SPRINT==> PRINTS View



  selected as


PR00691

Identifier
ADHESINB  [View Relations]  [View Alignment]  
Accession
PR00691
No. of Motifs
9
Creation Date
09-APR-1997  (UPDATE 22-JUN-1999)
Title
Adhesin B signature
Database References
PRINTS; PR00690 ADHESNFAMILY
INTERPRO; IPR001987
Literature References
1. SAMPSON, J.S., O'CONNOR, S.P., STINSON, A.R., THARPE, J.A. AND RUSSELL, H.
Cloning and nucleotide sequence analysis of psaA, the Streptococcus
pneumoniae gene encoding a 37-kilodalton protein homologous to previously
reported Streptococcus sp. adhesins.
INFECT.IMMUN. 62(1) 319-324 (1994).
 
2. GANESHKUMAR, N., HANNAM, P.M., KOLENBRANDER, P.E. AND MCBRIDE, B.C.
Nucleotide sequence of a gene coding for a saliva-binding protein (ssaB)
from Streptococcus sanguis 12 and possible role of the protein in
coaggregation with Actinomyces. 
INFECT.IMMUN. 59(3) 1093-1099 (1991).

Documentation
The Streptococcus pneumoniae psaA gene encodes a protein with significant
similarity to previously-reported Streptococcal proteins, SsaB (80%
similarity) and FimA (92.3% similarity), from S.sanguis and S.parasanguis
[1]. These homologues are associated with bacterial adhesion, and PsaA 
may play a similar role [1].
 
The SsaB protein has a putative hydrophobic 19-amino-acid signal sequence
yielding a 32,620-Mr secreted protein [2]. SsaB is hydrophilic and appears
not to have a hydrophobic membrane anchor in its C-terminal region. A high
degree of similarity exists between S.sanguis ssaB and type 1 fimbrial
genes [2]. Comparison of the gene products reveals close similarity of the
two proteins. It is thought that ssaB adhesion may play a role in oral
colonisation by binding either to a receptor on saliva or to a receptor
on Actinomyces. 
 
ADHESINB is a 9-element fingerprint that provides a signature for adhesins.
The fingerprint was derived from an initial alignment of 9 sequences: the
motifs were drawn from conserved regions spanning the full alignment
length, focusing on those sections that characterise the adhesin B
precursors but distinguish them from the rest of the adhesin family. A
single iteration on OWL29.2 was required to reach convergence, no further
sequences being identified beyond the starting set. Several partial matches
were also found, all of which are adhesin B homologues.
 
An update on SPTR37_9f identified a true set of 8 sequences, and 12
partial matches.
Summary Information
   8 codes involving  9 elements
0 codes involving 8 elements
0 codes involving 7 elements
3 codes involving 6 elements
0 codes involving 5 elements
4 codes involving 4 elements
1 codes involving 3 elements
4 codes involving 2 elements
Composite Feature Index
9888888888
8000000000
7000000000
6333333000
5000000000
4414030103
3101010000
2221200001
123456789
True Positives
ADHS_STRGC    ADHS_STRPA    ADHS_STRPN    ADHS_STRSA    
P72415 P72538 Q47723 Q53891
True Positive Partials
Codes involving 6 elements
Q55280 Q56952 Q57449
Codes involving 4 elements
O34385 O83077 P96116 Q56329
Codes involving 3 elements
Q47869
Codes involving 2 elements
O34966 O66870 O67917 P73085
Sequence Titles
ADHS_STRGC  COAGGREGATION-MEDIATING ADHESIN PRECURSOR - STREPTOCOCCUS GORDONII CHALLIS. 
ADHS_STRPA ADHESIN B PRECURSOR (SALIVA-BINDING PROTEIN) - STREPTOCOCCUS PARASANGUIS.
ADHS_STRPN ADHESION PROTEIN PRECURSOR - STREPTOCOCCUS PNEUMONIAE.
ADHS_STRSA ADHESIN B PRECURSOR (SALIVA-BINDING PROTEIN) - STREPTOCOCCUS SANGUIS.
P72415 LIPOPROTEIN - STAPHYLOCOCCUS EPIDERMIDIS.
P72538 SURFACE ADHESIN A PRECURSOR - STREPTOCOCCUS PNEUMONIAE.
Q47723 ENDOCARDITIS SPECIFIC ANTIGEN - ENTEROCOCCUS FAECALIS (STREPTOCOCCUS FAECALIS).
Q53891 SCBA - STREPTOCOCCUS CRISTATUS.

Q55280 PERIPLASMIC-BINDING PROTEIN - SYNECHOCYSTIS SP. (STRAIN PCC 6803).
Q56952 YFEA - YERSINIA PESTIS.
Q57449 HYPOTHETICAL PROTEIN HI0362 PRECURSOR - HAEMOPHILUS INFLUENZAE.

O34385 YTGA - BACILLUS SUBTILIS.
O83077 ABC TRANSPORTER, PERIPLASMIC BINDING PROTEIN - TREPONEMA PALLIDUM.
P96116 TROA PRECURSOR - TREPONEMA PALLIDUM.
Q56329 TROMP1 - TREPONEMA PALLIDUM.

Q47869 EWLA - ERYSIPELOTHRIX RHUSIOPATHIAE.

O34966 YCDH - BACILLUS SUBTILIS.
O66870 ADHESION B PRECURSOR - AQUIFEX AEOLICUS.
O67917 ADHESION PROTEIN - AQUIFEX AEOLICUS.
P73085 ADHESION PROTEIN - SYNECHOCYSTIS SP. (STRAIN PCC 6803).
Scan History
OWL29_2    1  50   NSINGLE    
SPTR37_9f 2 100 NSINGLE
Initial Motifs
Motif 1  width=22
Element Seqn Id St Int Rpt
KLKVVTTNSILADITKNIAGDK ADHS_STRPN 33 33 -
KLKVVATNSIIADITKNIAGDK ADHS_STRSA 32 32 -
KLKVVATNSIIADITKNIAGDK SPU407861 32 32 -
KLAIVTTNSILSDLVKNVGQDK EFU03756 30 30 -
KLKVVTTNSILYDMVKRVGGNK SEABCTS2 32 32 -
KLNVVATNSIIADITKNIAGDK SCU465421 33 33 -
KLKVVATNSIIADITKNIAGDK SPU53509 32 32 -
KLNVVATNSIIADITKNIAGDK ADHS_STRGC 33 33 -
KLKVVTTNSILADITKNIAGDK ADHS_STRPA 32 32 -

Motif 2 width=19
Element Seqn Id St Int Rpt
HSIVPIGTDPHEYEPLPED EFU03756 55 3 -
HSIVPVGQDPHKYEPLPED ADHS_STRGC 58 3 -
HSIVPVGKDPHEYEPLPED ADHS_STRPA 57 3 -
HSIVPIGQDPHDYEPLAED SPU407861 57 3 -
HSIVPVGQDPHEYEPLPED ADHS_STRPN 58 3 -
HSIVPIGQDPHEYEPLPED SPU53509 57 3 -
HSIVPVGQDPHEYEPLPED SCU465421 58 3 -
HSIVPVGKDPHEYEPLPED ADHS_STRSA 57 3 -
HSIVPVGQDPHEYEVKPKD SEABCTS2 57 3 -

Motif 3 width=20
Element Seqn Id St Int Rpt
VKKTSQADLIFYNGINLETG SCU465421 77 0 -
VKKTSQADLIFYNGINLETG ADHS_STRPA 76 0 -
VKKTSKADLIFYNGINLETG ADHS_STRGC 77 0 -
VKKTSEADLIFYNGINLETG SPU407861 76 0 -
VKKTSEADLIFYNGINLETG SPU53509 76 0 -
VKKTSQADLIFYNGINLETG ADHS_STRPN 77 0 -
IKALTDADVVFYNGLNLETG SEABCTS2 76 0 -
IAKASEADILFFNGLNLETG EFU03756 74 0 -
VKKTSQADLIFYNGINLETG ADHS_STRSA 76 0 -

Motif 4 width=20
Element Seqn Id St Int Rpt
EDPHAWLDIENGIKYVENIR EFU03756 134 40 -
QDPHAWLSLENGIKYVKTIQ SEABCTS2 137 41 -
EDPHAWLNLENGIIYAQNIA SCU465421 137 40 -
EDPHAWLNLENGIIFAKNIA SPU53509 136 40 -
EDPHAWLNLENGIIFAKNIA SPU407861 136 40 -
EDPHAWLNLENGILYAKNIA ADHS_STRPA 136 40 -
EDPHAWLNLENGIIYAKNIA ADHS_STRPN 137 40 -
EDPHAWLNLENGIIYAQNIA ADHS_STRSA 136 40 -
EDPHAWLNLENGIIYAQNIA ADHS_STRGC 137 40 -

Motif 5 width=18
Element Seqn Id St Int Rpt
IPDDKKLLVTSEGAFKYF EFU03756 192 38 -
IPKNQRAMMTSEGAFKYF SEABCTS2 195 38 -
IPGEKKMIVTSEGCFKYF SCU465421 195 38 -
IPAEKKLIVTSEGAFKYF SPU53509 194 38 -
IPEEKKMIVTSEGCPKYF ADHS_STRGC 195 38 -
IPEDKKMIVTSEGCFKYF ADHS_STRPA 194 38 -
IPAEKKMIVTSEGCFKYF ADHS_STRPN 195 38 -
IPEEKKMIVTSEGCFKYF ADHS_STRSA 194 38 -
IPAEKKLIVTSEGAFKYF SPU407861 194 38 -

Motif 6 width=19
Element Seqn Id St Int Rpt
AYIWEINTEEEGTPEQIKT SPU53509 220 8 -
GYIWEINTEKQGTPGQMKQ SEABCTS2 221 8 -
AYIWEINTESQGTPEQMTT EFU03756 218 8 -
AYIWEINTEEEGTPEQFKT SPU407861 220 8 -
AYIWEINTEEEGTPDQIKS ADHS_STRGC 221 8 -
AYIWEINTEEEGTPEQIKT ADHS_STRPA 220 8 -
AYIWEINTEVEGTPEQIKT ADHS_STRPN 221 8 -
AYIWEINTEEEGTPDQIKS ADHS_STRSA 220 8 -
AYIWEINTEEEGTPDQIKT SCU465421 221 8 -

Motif 7 width=18
Element Seqn Id St Int Rpt
LRQTKVPSLFVESSVDER ADHS_STRPN 244 4 -
LRQTKVPSLFVDSSVDDR SPU407861 243 4 -
LRQTKVPSLFVESSVDDR SPU53509 243 4 -
LRKTKVPSLFVESSVDDR SCU465421 244 4 -
VKDNHLKHLLVETSVDKK SEABCTS2 244 4 -
IKKSKAPVLFVETSVDKR EFU03756 241 4 -
LRKTKVPSLFVESSVDDR ADHS_STRSA 243 4 -
LRQTKVPALFVESSVDER ADHS_STRPA 243 4 -
LRKTKVPSLFVESSVDDR ADHS_STRGC 244 4 -

Motif 8 width=19
Element Seqn Id St Int Rpt
VSKDTNIPIYAKIFTDSVA SCU465421 266 4 -
VSKDTNIPIHAKIFTDSIA ADHS_STRSA 265 4 -
VSKDSNIPIFAKIFTDSIA ADHS_STRPN 266 4 -
VAKDTNIPIYAKIFTDSIA ADHS_STRPA 265 4 -
VSKDTNIPIYAKIFTDSIA ADHS_STRGC 266 4 -
VSQDTNIPIYAQIFTDSIA SPU53509 265 4 -
VSKEVKQPIYDTLFTDSLA EFU03756 263 4 -
VSQDTNIPIYAQIFTDSIA SPU407861 265 4 -
LSEETKKDIYGEVFTDSIG SEABCTS2 266 4 -

Motif 9 width=19
Element Seqn Id St Int Rpt
GEEGDSYYSMMKYNLEKIA SCU465421 287 2 -
GKEGDRYYSMMKYNLDKIA SPU407861 286 2 -
GEEGDSYYSMMKWNLEKIA ADHS_STRPN 287 2 -
GEKGDSYYSMMKWNLDKIA ADHS_STRPA 286 2 -
GEDGDSYYSMMKYNLDKIS ADHS_STRGC 287 2 -
GEEGDTYYSMMKYNLDKIS ADHS_STRSA 286 2 -
GTEGDTYYSMMNWNLTKIH EFU03756 284 2 -
GTKGDSYYKMMKSNIDTIH SEABCTS2 287 2 -
GKEGDSYYSMMKYNLDKIA SPU53509 286 2 -
Final Motifs
Motif 1  width=22
Element Seqn Id St Int Rpt
KLNVVATNSIIADITKNIAGDK Q53891 33 33 -
KLKVVATNSIIADITKNIAGDK ADHS_STRSA 32 32 -
KLKVVTTNSILADITKNIAGDK ADHS_STRPN 33 33 -
KLKVVTTNSILADITKNIAGDK ADHS_STRPA 32 32 -
KLNVVATNSIIADITKNIAGDK ADHS_STRGC 33 33 -
KLKVVATNSIIADITKNIAGDK P72538 32 32 -
KLAIVTTNSILSDLVKNVGQDK Q47723 30 30 -
KLKVVTTNSILYDMVKRVGGNK P72415 32 32 -

Motif 2 width=19
Element Seqn Id St Int Rpt
HSIVPVGQDPHEYEPLPED Q53891 58 3 -
HSIVPVGKDPHEYEPLPED ADHS_STRSA 57 3 -
HSIVPVGQDPHEYEPLPED ADHS_STRPN 58 3 -
HSIVPVGKDPHEYEPLPED ADHS_STRPA 57 3 -
HSIVPVGQDPHKYEPLPED ADHS_STRGC 58 3 -
HSIVPIGQDPHEYEPLPED P72538 57 3 -
HSIVPIGTDPHEYEPLPED Q47723 55 3 -
HSIVPVGQDPHEYEVKPKD P72415 57 3 -

Motif 3 width=20
Element Seqn Id St Int Rpt
VKKTSQADLIFYNGINLETG Q53891 77 0 -
VKKTSQADLIFYNGINLETG ADHS_STRSA 76 0 -
VKKTSQADLIFYNGINLETG ADHS_STRPN 77 0 -
VKKTSQADLIFYNGINLETG ADHS_STRPA 76 0 -
VKKTSKADLIFYNGINLETG ADHS_STRGC 77 0 -
VKKTSEADLIFYNGINLETG P72538 76 0 -
IAKASEADILFFNGLNLETG Q47723 74 0 -
IKALTDADVVFYNGLNLETG P72415 76 0 -

Motif 4 width=20
Element Seqn Id St Int Rpt
EDPHAWLNLENGIIYAQNIA Q53891 137 40 -
EDPHAWLNLENGIIYAQNIA ADHS_STRSA 136 40 -
EDPHAWLNLENGIIYAKNIA ADHS_STRPN 137 40 -
EDPHAWLNLENGILYAKNIA ADHS_STRPA 136 40 -
EDPHAWLNLENGIIYAQNIA ADHS_STRGC 137 40 -
EDPHAWLNLENGIIFAKNIA P72538 136 40 -
EDPHAWLDIENGIKYVENIR Q47723 134 40 -
QDPHAWLSLENGIKYVKTIQ P72415 137 41 -

Motif 5 width=18
Element Seqn Id St Int Rpt
IPGEKKMIVTSEGCFKYF Q53891 195 38 -
IPEEKKMIVTSEGCFKYF ADHS_STRSA 194 38 -
IPAEKKMIVTSEGCFKYF ADHS_STRPN 195 38 -
IPEDKKMIVTSEGCFKYF ADHS_STRPA 194 38 -
IPEEKKMIVTSEGCPKYF ADHS_STRGC 195 38 -
IPAEKKLIVTSEGAFKYF P72538 194 38 -
IPDDKKLLVTSEGAFKYF Q47723 192 38 -
IPKNQRAMMTSEGAFKYF P72415 195 38 -

Motif 6 width=19
Element Seqn Id St Int Rpt
AYIWEINTEEEGTPDQIKT Q53891 221 8 -
AYIWEINTEEEGTPDQIKS ADHS_STRSA 220 8 -
AYIWEINTEVEGTPEQIKT ADHS_STRPN 221 8 -
AYIWEINTEEEGTPEQIKT ADHS_STRPA 220 8 -
AYIWEINTEEEGTPDQIKS ADHS_STRGC 221 8 -
AYIWEINTEEEGTPEQIKT P72538 220 8 -
AYIWEINTESQGTPEQMTT Q47723 218 8 -
GYIWEINTEKQGTPGQMKQ P72415 221 8 -

Motif 7 width=18
Element Seqn Id St Int Rpt
LRKTKVPSLFVESSVDDR Q53891 244 4 -
LRKTKVPSLFVESSVDDR ADHS_STRSA 243 4 -
LRQTKVPSLFVESSVDER ADHS_STRPN 244 4 -
LRQTKVPALFVESSVDER ADHS_STRPA 243 4 -
LRKTKVPSLFVESSVDDR ADHS_STRGC 244 4 -
LRQTKVPSLFVESSVDDR P72538 243 4 -
IKKSKAPVLFVETSVDKR Q47723 241 4 -
VKDNHLKHLLVETSVDKK P72415 244 4 -

Motif 8 width=19
Element Seqn Id St Int Rpt
VSKDTNIPIYAKIFTDSVA Q53891 266 4 -
VSKDTNIPIHAKIFTDSIA ADHS_STRSA 265 4 -
VSKDSNIPIFAKIFTDSIA ADHS_STRPN 266 4 -
VAKDTNIPIYAKIFTDSIA ADHS_STRPA 265 4 -
VSKDTNIPIYAKIFTDSIA ADHS_STRGC 266 4 -
VSQDTNIPIYAQIFTDSIA P72538 265 4 -
VSKEVKQPIYDTLFTDSLA Q47723 263 4 -
LSEETKKDIYGEVFTDSIG P72415 266 4 -

Motif 9 width=19
Element Seqn Id St Int Rpt
GEEGDSYYSMMKYNLEKIA Q53891 287 2 -
GEEGDTYYSMMKYNLDKIS ADHS_STRSA 286 2 -
GEEGDSYYSMMKWNLEKIA ADHS_STRPN 287 2 -
GEKGDSYYSMMKWNLDKIA ADHS_STRPA 286 2 -
GEDGDSYYSMMKYNLDKIS ADHS_STRGC 287 2 -
GKEGDSYYSMMKYNLDKIA P72538 286 2 -
GTEGDTYYSMMNWNLTKIH Q47723 284 2 -
GTKGDSYYKMMKSNIDTIH P72415 287 2 -