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PR00683

Identifier
SPECTRINPH  [View Relations]  [View Alignment]  
Accession
PR00683
No. of Motifs
4
Creation Date
16-JAN-1997  (UPDATE 07-JUN-1999)
Title
Spectrin pleckstrin homology domain signature
Database References

PROSITE; PS50003 PH_DOMAIN
BLOCKS; BL50003
PFAM; PF00169 PH
INTERPRO; IPR001605
PDB; 1DRO
SCOP; 1DRO
Literature References
1. MACIAS, M.J, MUSACCHIO, A., PONSTINGL, H., NILGES, M., SARASTE, M. 
AND OSCHKINAT, H.
Structure of the pleckstrin homology domain from B-spectrin.       
NATURE 369 675-677 (1994).
 
2. HO SUP YOON, HAJDUK, P., PETROS, A., OLEJNICZAK, E., MEADOWS, R. 
AND FESIK, S. 
Solution structure of a pleckstrin homology domain.
NATURE 369 672-675 (1994).
 
3. ZHOU, M., HUANG, B., OLEJNICZAK, E., MEADOWS, R., SHUKER, S., 
MIYAZAKI, M., TRUB, T., SHOELSON, S. AND  FESIK, S.
Structural basis for IL-4 receptor phosphopeptide recognition by the IRS-1
PTB domain.
NAT.STRUCT.BIOL. 3 388-393 (1996). 

Documentation
Spectrin is the major constituent of the cytoskeletal network underlying 
the erythrocyte plasma membrane; it associates with band 4.1 and actin to 
form the cytoskeletal super-structure. The native spectrin molecule is a
tetramer comprising two anti-parallel heterodimers joined head to head,
such that the C-terminus of the alpha-subunit and the N-terminus of the
beta-subunit are included within the same molecule.
 
Spectrin is similar to alpha-actinin and dystrophin, and contains a
pleckstrin homology (PH) domain. The exact function of the PH domain is
unknown, but evidence suggests that it contains an electrostatically-
polarised pocket that facilitates binding of a ligand (e.g., a peptide).
The PH domain contains a number of hydrophobic residues that form a
hydrophobic core responsible for protein stability. The spectrin PH
domain [1], which has the familiar 7-stranded anti-parallel up and down
beta-barrel capped by a C-terminal amphiphilic alpha helical cap [2],
contains insertions that confer 2 additional turns of alpha-helix in the
loop between strands 3 and 4. The C-terminal helix is packed into a gorge
between strands 1 and 2, and lies parallel to strand 7 [3].
 
SPECTRINPH is a 4-element fingerprint that provides a signature for the
spectrin PH domain. The fingerprint was derived from an initial alignment
of 6 sequences: motif 1 encodes strand 1; motif 2 spans strands 2, 3 and
part of the additional alpha-helix; motif 3 spans strands 5 and 6; and
motif 4 spans strand 7 and part of the C-terminal helix. Two iterations
on OWL 28.3 were required to reach convergence, at which point a true set
comprising 8 sequences was identified. A single partial matches was also
found, HSTYL, a human TYL gene product that matches motifs 3 and 4.
 
An update on SPTR37_9f identified a true set of 9 sequences.
Summary Information
9 codes involving  4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
49999
30000
20000
1234
True Positives
O02425        O15020        O61612        O88197        
Q22014 Q94275 SPCB_DROME SPCO_HUMAN
SPCO_MOUSE
Sequence Titles
O02425      R31.1 PROTEIN - CAENORHABDITIS ELEGANS.       
O15020 BETA-SPECTRIN III - HOMO SAPIENS (HUMAN).
O61612 BETA CHAIN SPECTRIN HOMOLOG SMA1 - CAENORHABDITIS ELEGANS.
O88197 BETA-SPECTRIN III - RATTUS NORVEGICUS (RAT).
Q22014 R31.1 PROTEIN - CAENORHABDITIS ELEGANS.
Q94275 SIMILAR TO SPECTRIN BETA CHAIN - CAENORHABDITIS ELEGANS.
SPCB_DROME SPECTRIN BETA CHAIN - DROSOPHILA MELANOGASTER (FRUIT FLY).
SPCO_HUMAN SPECTRIN BETA CHAIN, BRAIN (SPECTRIN, NON-ERYTHROID BETA CHAIN) (FODRIN BETA CHAIN) (SPTBN1) - HOMO SAPIENS (HUMAN).
SPCO_MOUSE SPECTRIN BETA CHAIN, BRAIN (SPECTRIN, NON-ERYTHROID BETA CHAIN) (FODRIN BETA CHAIN) (SPTBN1) - MUS MUSCULUS (MOUSE).
Scan History
OWL28_3    2  100  NSINGLE    
SPTR37_9f 2 11 NSINGLE
Initial Motifs
Motif 1  width=20
Element Seqn Id St Int Rpt
MEGFLNRKHEWEAHNKKASS A44159 2200 2200 -
MEGYLGRKHDLEGPNKKASN A23659 116 116 -
MEGYLGRKHDLEGPNKKASN HUMSPC1 1127 1127 -
HEGYVTRKHEWDSTTKKASN SPCB_DROME 2150 2150 -
MEGFLNRKHEWEAHNKKASS S29854 543 543 -
MEGFLNRKHEWEAHNKKASS MUSSPNA 2199 2199 -

Motif 2 width=22
Element Seqn Id St Int Rpt
RSWHNVYCVINNQEMGFYKDAK S29854 563 0 -
RSWHNVYCVINNQEMGFYKDAK MUSSPNA 2219 0 -
RSWNNLYCVLRNSELTFYKDAK A23659 136 0 -
RSWDKVYMAAKAGRISFYKDQK SPCB_DROME 2170 0 -
RSWNNLYCVLRNSELTFYKDAK HUMSPC1 1147 0 -
RSWHNVYCVINNQEMGFYKDAK A44159 2220 0 -

Motif 3 width=18
Element Seqn Id St Int Rpt
CEVALDYKKKKHVFKLRL MUSSPNA 2261 20 -
CEVALDYKKKKHVFKLRL A44159 2262 20 -
CEVALDYKKKKHVFKLRL S29854 605 20 -
CEIAANYKKKKHVFKLRL A23659 178 20 -
CEIAANYKKKKHVFKLRL HUMSPC1 1189 20 -
IEIASDYTKKKHVLRVKL SPCB_DROME 2214 22 -

Motif 4 width=19
Element Seqn Id St Int Rpt
GSEWLFHGKDEEEMLSWLQ A23659 198 2 -
GSEWLFHGKDEEEMLSWLQ HUMSPC1 1209 2 -
GNEYLFQAKDDEEMNTWIQ S29854 625 2 -
GNEYLFQAKDDEEMNTWIQ A44159 2282 2 -
GALFLLQAHDDTEMSQWVT SPCB_DROME 2234 2 -
GNEYLFQAKDDEEMNTWIQ MUSSPNA 2281 2 -
Final Motifs
Motif 1  width=20
Element Seqn Id St Int Rpt
MEGMLCRKQEMEAFGKKAAN O15020 2221 2221 -
MEGFLNRKHEWEAHNKKASS SPCO_HUMAN 2200 2200 -
MEGFLNRKHEWEAHNKKASS SPCO_MOUSE 2199 2199 -
MEGTLCRKQEMEAFNKKAAN O88197 2221 2221 -
MKGFFDRKQCQQSGGKRATI O02425 3699 3699 -
MKGFFDRKQCQQSGGKRATI O61612 3677 3677 -
MKGFFDRKQCQQSGGKRATI Q22014 2163 2163 -
FEGTLIRKHTYESLDRKAAN Q94275 2189 2189 -
HEGYVTRKHEWDSTTKKASN SPCB_DROME 2150 2150 -

Motif 2 width=22
Element Seqn Id St Int Rpt
RSWQNVYCVLRRGSLGFYKDAK O15020 2241 0 -
RSWHNVYCVINNQEMGFYKDAK SPCO_HUMAN 2220 0 -
RSWHNVYCVINNQEMGFYKDAK SPCO_MOUSE 2219 0 -
RSWQNVYCVLRRGSLGFYKDAR O88197 2241 0 -
RSWKNYYGILCGQLLCFFKDEQ O02425 3719 0 -
RSWKNYYGILCGQLLCFFKDEQ O61612 3697 0 -
RSWKNYYGILCGQLLCFFKDEQ Q22014 2183 0 -
RSWEKLYAVLRQNELSFYKDPK Q94275 2209 0 -
RSWDKVYMAAKAGRISFYKDQK SPCB_DROME 2170 0 -

Motif 3 width=18
Element Seqn Id St Int Rpt
GSVAFDYRKRKHVFKLGL O15020 2283 20 -
CEVALDYKKKKHVFKLRL SPCO_HUMAN 2262 20 -
CEVALDYKKKKHVFKLRL SPCO_MOUSE 2261 20 -
GSVAFDYRKRKHVFKLGL O88197 2283 20 -
CEQYPEYAKRKNSFRLLL O02425 3759 18 -
CEQYPEYAKRKNSFRLLL O61612 3737 18 -
CEQYPEYAKRKNSFRLLL Q22014 2223 18 -
VNVASDYQKKKNVLSLRL Q94275 2249 18 -
IEIASDYTKKKHVLRVKL SPCB_DROME 2214 22 -

Motif 4 width=19
Element Seqn Id St Int Rpt
GKEYLFQAKDEAEMSSWLR O15020 2303 2 -
GNEYLFQAKDDEEMNTWIQ SPCO_HUMAN 2282 2 -
GNEYLFQAKDDEEMNTWIQ SPCO_MOUSE 2281 2 -
GKEYLFQAKDEAEMSSWLR O88197 2303 2 -
GSEFIFSCPDERQMLEWVA O02425 3779 2 -
GSEFIFSCPDERQMLEWVA O61612 3757 2 -
GSEFIFSCPDERQMLEWVA Q22014 2243 2 -
GAEYLFQCGSEEDMQRWLT Q94275 2269 2 -
GALFLLQAHDDTEMSQWVT SPCB_DROME 2234 2 -