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PR00537

Identifier
MUOPIOIDR  [View Relations]  [View Alignment]  
Accession
PR00537
No. of Motifs
7
Creation Date
19-MAY-1996  (UPDATE 07-JUN-1999)
Title
Mu opioid receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR00384 OPIOIDR
INTERPRO; IPR000105
GCRDB; GCR_0885; GCR_0966
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL, S.
Opioid peptides.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.205-213.

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
The term opioid refers to a class of substance that produces its effects
via the major classes of opioid receptor, termed mu, delta and kappa [6].
In the CNS, the mu opioid receptor is found in the cerebral cortex,
thalamus, hypothalamus, periaqueductal grey, interpeduncular nucleus and
median raphe. In the periphery, it is found in the myenteric plexus, and
in certain smooth muscles, e.g. mouse vas deferens [6]. Mu opioid
receptors are believed to mediate analgesia, hypothermia, respiratory
depression, miosis, bradycardia, nausea, euphoria and physical dependence.
Beta-endorphin is the most potent endogenous ligand.
 
MUOPIOIDR is a 7-element fingerprint that provides a signature for the 
mu opioid receptors. The fingerprint was derived from an initial
alignment of 3 sequences: the motifs were drawn from conserved sections
within either loop or N- and C-terminal regions, focusing on those areas
of the alignment that characterise the mu opioid receptors but distinguish
them from the rest of the opioid receptor family - motifs 1-3 were drawn
from the N-terminal region preceding TM domain 1; motif 3 spans the
C-terminus of TM domain 4 leading into the second external loop; motif 5
spans the third external loop; and motifs 6 and 7 were drawn from the
C-terminal region following TM domain 7. Two iterations on OWL27.1 were
required to reach convergence, at which point a true set comprising 5
sequences was identified.
 
An update on SPTR37_9f identified a true set of 5 sequences.
Summary Information
5 codes involving  7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
75555555
60000000
50000000
40000000
30000000
20000000
1234567
True Positives
OPRM_BOVIN    OPRM_HUMAN    OPRM_MOUSE    OPRM_PIG      
OPRM_RAT
Sequence Titles
OPRM_BOVIN  Mu-type opioid receptor (MOR-1) - Bos taurus (Bovine). 
OPRM_HUMAN Mu-type opioid receptor (MOR-1) - Homo sapiens (Human).
OPRM_MOUSE Mu-type opioid receptor (MOR-1) - Mus musculus (Mouse).
OPRM_PIG Mu-type opioid receptor (MOR-1) - Sus scrofa (Pig).
OPRM_RAT Mu-type opioid receptor (MOR-1) (Opioid receptor B) (MUOR1) - Rattus norvegicus (Rat).
Scan History
OWL27_1    2  100  NSINGLE    
SPTR37_9f 2 180 NSINGLE
Initial Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
CSDPLAPASCSPAP OPRM_MOUSE 13 13 -
CSDPLAQASCSPAP OPRM_RAT 13 13 -
CTDALAYSSCSPAP OPRM_HUMAN 13 13 -

Motif 2 width=17
Element Seqn Id St Int Rpt
GSWLNLSHVDGNQSDPC OPRM_MOUSE 27 0 -
GSWLNLSHVDGNQSDPC OPRM_RAT 27 0 -
GSWVNLSHLDGNLSDPC OPRM_HUMAN 29 2 -

Motif 3 width=15
Element Seqn Id St Int Rpt
CGPNRTGLGGSHSLC OPRM_MOUSE 43 -1 -
CGLNRTGLGGNDSLC OPRM_RAT 43 -1 -
CGPNRTNLGGRDSLC OPRM_HUMAN 45 -1 -

Motif 4 width=14
Element Seqn Id St Int Rpt
MFMATTKYRQGSID OPRM_MOUSE 203 145 -
MFMATTKYRQGSID OPRM_RAT 203 145 -
MFMATTKYRQGSID OPRM_HUMAN 205 145 -

Motif 5 width=18
Element Seqn Id St Int Rpt
KALITIPETTFQTVSWHF OPRM_MOUSE 303 86 -
KALITIPETTFQTVSWHF OPRM_RAT 303 86 -
KALVTIPETTFQTVSWHF OPRM_HUMAN 305 86 -

Motif 6 width=13
Element Seqn Id St Int Rpt
IPTSSTIEQQNSA OPRM_MOUSE 352 31 -
IPTSSTIEQQNST OPRM_RAT 352 31 -
IPTSSNIEQQNST OPRM_HUMAN 354 31 -

Motif 7 width=15
Element Seqn Id St Int Rpt
HPSTANTVDRTNHQL OPRM_MOUSE 373 8 -
HPSTANTVDRTNHQL OPRM_RAT 373 8 -
HPSTANTVDRTNHQL OPRM_HUMAN 375 8 -
Final Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
CSDPLAPASCSPAP OPRM_MOUSE 13 13 -
CSDPLAQASCSPAP OPRM_RAT 13 13 -
CTDALAYSSCSPAP OPRM_HUMAN 13 13 -
TDPFTHPSSCSPAP OPRM_BOVIN 14 14 -
TDPFSPSSMCSPVP OPRM_PIG 14 14 -

Motif 2 width=17
Element Seqn Id St Int Rpt
GSWLNLSHVDGNQSDPC OPRM_MOUSE 27 0 -
GSWLNLSHVDGNQSDPC OPRM_RAT 27 0 -
GSWVNLSHLDGNLSDPC OPRM_HUMAN 29 2 -
SSWVNFSHLEGNLSDPC OPRM_BOVIN 30 2 -
SSWVNFSHLEGNLSDPC OPRM_PIG 30 2 -

Motif 3 width=15
Element Seqn Id St Int Rpt
CGPNRTGLGGSHSLC OPRM_MOUSE 43 -1 -
CGLNRTGLGGNDSLC OPRM_RAT 43 -1 -
CGPNRTNLGGRDSLC OPRM_HUMAN 45 -1 -
CGPNRTELGGSDRLC OPRM_BOVIN 46 -1 -
CIRNRTELGGSDSLC OPRM_PIG 46 -1 -

Motif 4 width=14
Element Seqn Id St Int Rpt
MFMATTKYRQGSID OPRM_MOUSE 203 145 -
MFMATTKYRQGSID OPRM_RAT 203 145 -
MFMATTKYRQGSID OPRM_HUMAN 205 145 -
MFMATTKYRQGSID OPRM_BOVIN 206 145 -
MFMATTKYRNGSID OPRM_PIG 206 145 -

Motif 5 width=18
Element Seqn Id St Int Rpt
KALITIPETTFQTVSWHF OPRM_MOUSE 303 86 -
KALITIPETTFQTVSWHF OPRM_RAT 303 86 -
KALVTIPETTFQTVSWHF OPRM_HUMAN 305 86 -
KALITIPETTFQTVSWHF OPRM_BOVIN 306 86 -
KALITIPETTFQTVSWHF OPRM_PIG 306 86 -

Motif 6 width=13
Element Seqn Id St Int Rpt
IPTSSTIEQQNSA OPRM_MOUSE 352 31 -
IPTSSTIEQQNST OPRM_RAT 352 31 -
IPTSSNIEQQNST OPRM_HUMAN 354 31 -
IPTSSTIEQQNST OPRM_BOVIN 355 31 -
IPTSSTIEQQNSA OPRM_PIG 355 31 -

Motif 7 width=15
Element Seqn Id St Int Rpt
HPSTANTVDRTNHQL OPRM_MOUSE 373 8 -
HPSTANTVDRTNHQL OPRM_RAT 373 8 -
HPSTANTVDRTNHQL OPRM_HUMAN 375 8 -
HPSTANTVDRTNHQL OPRM_BOVIN 376 8 -
HPSTANTVDRTNHQL OPRM_PIG 376 8 -