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PR00532

Identifier
KAPPAOPIOIDR  [View Relations]  [View Alignment]  
Accession
PR00532
No. of Motifs
7
Creation Date
19-MAY-1996  (UPDATE 07-JUN-1999)
Title
Kappa opioid receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR00384 OPIOIDR
INTERPRO; IPR000452
GCRDB; GCR_0991
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL, S.
Opioid peptides.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.205-213.

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
The term opioid refers to a class of substance that produces its effects
via the major classes of opioid receptor, termed mu, delta and kappa [6].
In the CNS, the kappa opioid receptor is found in the cerebral cortex,
substantia nigra, interpeduncular nucleus, striatum and hippocampus. In
the periphery, it is found in the myenteric plexus of the guinea pig ileum,
and it is also in certain smooth muscles, e.g. rabbit vas deferens [6].
K-opioid receptors are believed to mediate analgesia, sedation, miosis and
diuresis. Dynorphin is the most potent endogenous ligand.     
 
KAPPAOPIOIDR is a 7-element fingerprint that provides a signature for the 
kappa opioid receptors. The fingerprint was derived from an initial
alignment of 4 sequences: the motifs were drawn from conserved sections
within either loop or N- and C-terminal regions, focusing on those areas
of the alignment that characterise the K-opioid receptors but distinguish
them from the rest of the opioid receptor family - motifs 1-3 were drawn
from the N-terminal region preceding TM domain 1; motifs 4 and 5 span the
second external loop; motif 6 spans the third external loop leading into
TM domain 7; and motif 7 was drawn from the C-terminus. A single iteration
on OWL27.1 was required to reach convergence, no further sequences being
identified beyond the starting set.
 
An update on SPTR37_9f identified a true set of 4 sequences.
Summary Information
4 codes involving  7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
74444444
60000000
50000000
40000000
30000000
20000000
1234567
True Positives
OPRK_CAVPO    OPRK_HUMAN    OPRK_MOUSE    OPRK_RAT      
Sequence Titles
OPRK_CAVPO  Kappa-type opioid receptor (KOR-1) - Cavia porcellus (Guinea pig). 
OPRK_HUMAN Kappa-type opioid receptor (KOR-1) - Homo sapiens (Human).
OPRK_MOUSE Kappa-type opioid receptor (KOR-1) (MSL-1) - Mus musculus (Mouse).
OPRK_RAT Kappa-type opioid receptor (KOR-1) - Rattus norvegicus (Rat).
Scan History
OWL27_1    1  50   NSINGLE    
SPTR37_9f 2 5 NSINGLE
Initial Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
ACLLPNSSSWFPNWAE OPRK_RAT 20 20 -
ACLLPNSSSWFPNWAE OPRK_MOUSE 20 20 -
ACLPPNSSAWFPGWAE OPRK_HUMAN 20 20 -
ACLLPNGSAWLPGWAE OPRK_CAVPO 20 20 -

Motif 2 width=14
Element Seqn Id St Int Rpt
DSNGSVGSEDQQLE OPRK_RAT 37 1 -
DSNGSVGSEDQQLE OPRK_MOUSE 37 1 -
DSNGSAGSEDAQLE OPRK_HUMAN 37 1 -
DGNGSAGPQDEQLE OPRK_CAVPO 37 1 -

Motif 3 width=13
Element Seqn Id St Int Rpt
EPAHISPAIPVII OPRK_RAT 50 -1 -
ESAHISPAIPVII OPRK_MOUSE 50 -1 -
EPAHISPAIPVII OPRK_HUMAN 50 -1 -
EPAHISPAIPVII OPRK_CAVPO 50 -1 -

Motif 4 width=14
Element Seqn Id St Int Rpt
LGGTKVREDVDVIE OPRK_RAT 196 133 -
LGGTKVREDVDVIE OPRK_MOUSE 196 133 -
LGGTKVREDVDVIE OPRK_HUMAN 196 133 -
LGGTKVREDVDIIE OPRK_CAVPO 196 133 -

Motif 5 width=11
Element Seqn Id St Int Rpt
DDEYSWWDLFM OPRK_RAT 216 6 -
DDEYSWWDLFM OPRK_MOUSE 216 6 -
DDDYSWWDLFM OPRK_HUMAN 216 6 -
DDDYSWWDLFM OPRK_CAVPO 216 6 -

Motif 6 width=18
Element Seqn Id St Int Rpt
EALGSTSHSTAVLSSYYF OPRK_RAT 297 70 -
EALGSTSHSTAALSSYYF OPRK_MOUSE 297 70 -
EALGSTSHSTAALSSYYF OPRK_HUMAN 297 70 -
EALGSTSHSTAALSSYYF OPRK_CAVPO 297 70 -

Motif 7 width=16
Element Seqn Id St Int Rpt
VQDPASMRDVGGMNKP OPRK_RAT 364 49 -
VQDPASMRDVGGMNKP OPRK_MOUSE 364 49 -
VQDPAYLRDIDGMNKP OPRK_HUMAN 364 49 -
VQDPAYMRNVDGVNKP OPRK_CAVPO 364 49 -
Final Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
ACLLPNSSSWFPNWAE OPRK_RAT 20 20 -
ACLLPNSSSWFPNWAE OPRK_MOUSE 20 20 -
ACLPPNSSAWFPGWAE OPRK_HUMAN 20 20 -
ACLLPNGSAWLPGWAE OPRK_CAVPO 20 20 -

Motif 2 width=14
Element Seqn Id St Int Rpt
DSNGSVGSEDQQLE OPRK_RAT 37 1 -
DSNGSVGSEDQQLE OPRK_MOUSE 37 1 -
DSNGSAGSEDAQLE OPRK_HUMAN 37 1 -
DGNGSAGPQDEQLE OPRK_CAVPO 37 1 -

Motif 3 width=13
Element Seqn Id St Int Rpt
EPAHISPAIPVII OPRK_RAT 50 -1 -
ESAHISPAIPVII OPRK_MOUSE 50 -1 -
EPAHISPAIPVII OPRK_HUMAN 50 -1 -
EPAHISPAIPVII OPRK_CAVPO 50 -1 -

Motif 4 width=14
Element Seqn Id St Int Rpt
LGGTKVREDVDVIE OPRK_RAT 196 133 -
LGGTKVREDVDVIE OPRK_MOUSE 196 133 -
LGGTKVREDVDVIE OPRK_HUMAN 196 133 -
LGGTKVREDVDIIE OPRK_CAVPO 196 133 -

Motif 5 width=11
Element Seqn Id St Int Rpt
DDEYSWWDLFM OPRK_RAT 216 6 -
DDEYSWWDLFM OPRK_MOUSE 216 6 -
DDDYSWWDLFM OPRK_HUMAN 216 6 -
DDDYSWWDLFM OPRK_CAVPO 216 6 -

Motif 6 width=18
Element Seqn Id St Int Rpt
EALGSTSHSTAVLSSYYF OPRK_RAT 297 70 -
EALGSTSHSTAALSSYYF OPRK_MOUSE 297 70 -
EALGSTSHSTAALSSYYF OPRK_HUMAN 297 70 -
EALGSTSHSTAALSSYYF OPRK_CAVPO 297 70 -

Motif 7 width=16
Element Seqn Id St Int Rpt
VQDPASMRDVGGMNKP OPRK_RAT 364 49 -
VQDPASMRDVGGMNKP OPRK_MOUSE 364 49 -
VQDPAYLRDIDGMNKP OPRK_HUMAN 364 49 -
VQDPAYMRNVDGVNKP OPRK_CAVPO 364 49 -