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PR00384

Identifier
OPIOIDR  [View Relations]  [View Alignment]  
Accession
PR00384
No. of Motifs
6
Creation Date
05-JUL-1995  (UPDATE 29-JUN-1999)
Title
Opioid receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR00525 DELTAOPIOIDR; PR00532 KAPPAOPIOIDR; PR00537 MUOPIOIDR
PRINTS; PR00547 XOPIOIDR
INTERPRO; IPR001418
GCRDB; GCR_0633; GCR_0640; GCR_0864; GCR_0991
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL, S.
Opioid peptides.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.205-213.

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
The term opioid refers to a class of substance that produces its effects
via the major classes of opioid receptor, termed mu, delta and kappa [6].
The receptors are found in the CNS and certain smooth muscles: mu-opioid
receptors are believed to mediate analgesia, hypothermia, respiratory
depression, miosis, bradycardia, nausea, euphoria and physical dependence,
beta-endorphin being the most potent endogenous ligand; delta-receptors
mediate analgesia; and kappa-opioid receptors are believed to mediate
analgesia, sedation, miosis and diuresis, dynorphin being the most potent
endogenous ligand.
 
A human placental protein that binds opioid ligands with moderate affinity
has been provisionally classified as an opioid receptor [6]. However, it is
not selective for kappa-ligands and is 93% similar to the human NK3
tachykinin receptor, although it doesn't bind tachykinins.   
 
OPIOIDR is a 6-element fingerprint that provides a signature for the opioid
receptors. The fingerprint was derived from an initial alignment of 4
sequences: the motifs were drawn from conserved sections within either loop
or TM regions, focusing on those areas of the alignment that characterise
the opioid receptors but distinguish them from the rest of the rhodopsin-
like superfamily - motif 1 spans part of the first cytoplasmic loop and
TM domain 2; motif 2 lies in TM domain 2; motifs 3 and 4 span the second
and third cytoplasmic loops res.; and motifs 5 and 6 lie at the N- and
C-termini of TM domain 7. Two iterations on OWL26.0 were required to reach
convergence, at which point a true set comprising 20 sequences was
identfied. Several partial matches were also found, all of which are
somatostatin receptors that match motifs 1, 2 and 6. Notably, the putative
placental opioid receptor was not identified by this signature.
 
An update on SPTR37_9f identified a true set of 18 sequences, and 17
partial matches.
Summary Information
  18 codes involving  6 elements
1 codes involving 5 elements
0 codes involving 4 elements
8 codes involving 3 elements
8 codes involving 2 elements
Composite Feature Index
6181818181818
5111101
4000000
3880008
2870001
123456
True Positives
O42324        OPRD_HUMAN    OPRD_MOUSE    OPRD_RAT      
OPRK_CAVPO OPRK_HUMAN OPRK_MOUSE OPRK_RAT
OPRM_BOVIN OPRM_HUMAN OPRM_MOUSE OPRM_PIG
OPRM_RAT OPRX_CAVPO OPRX_HUMAN OPRX_MOUSE
OPRX_PIG OPRX_RAT
True Positive Partials
Codes involving 5 elements
O57585
Codes involving 3 elements
SSR1_HUMAN SSR1_MOUSE SSR1_RAT SSR2_BOVIN
SSR2_HUMAN SSR2_MOUSE SSR2_PIG SSR2_RAT
Codes involving 2 elements
GPR8_HUMAN SSR4_HUMAN SSR4_MOUSE SSR4_RAT
SSR5_HUMAN SSR5_MOUSE SSR5_RAT SSRL_FUGRU
Sequence Titles
O42324      MU-OPIOID RECEPTOR - CATOSTOMUS COMMERSONI (WHITE SUCKER). 
OPRD_HUMAN Delta-type opioid receptor (DOR-1) - Homo sapiens (Human).
OPRD_MOUSE Delta-type opioid receptor (DOR-1) (K56) (MSL-2) - Mus musculus (Mouse).
OPRD_RAT Delta-type opioid receptor (DOR-1) (Opioid receptor A) - Rattus norvegicus (Rat).
OPRK_CAVPO Kappa-type opioid receptor (KOR-1) - Cavia porcellus (Guinea pig).
OPRK_HUMAN Kappa-type opioid receptor (KOR-1) - Homo sapiens (Human).
OPRK_MOUSE Kappa-type opioid receptor (KOR-1) (MSL-1) - Mus musculus (Mouse).
OPRK_RAT Kappa-type opioid receptor (KOR-1) - Rattus norvegicus (Rat).
OPRM_BOVIN Mu-type opioid receptor (MOR-1) - Bos taurus (Bovine).
OPRM_HUMAN Mu-type opioid receptor (MOR-1) - Homo sapiens (Human).
OPRM_MOUSE Mu-type opioid receptor (MOR-1) - Mus musculus (Mouse).
OPRM_PIG Mu-type opioid receptor (MOR-1) - Sus scrofa (Pig).
OPRM_RAT Mu-type opioid receptor (MOR-1) (Opioid receptor B) (MUOR1) - Rattus norvegicus (Rat).
OPRX_CAVPO Nociceptin receptor (Orphanin FQ receptor) (Kappa-type 3 opioid receptor) (KOR-3) (XOR) - Cavia porcellus (Guinea pig).
OPRX_HUMAN Nociceptin receptor (Orphanin FQ receptor) (Kappa-type 3 opioid receptor) (KOR-3) - Homo sapiens (Human).
OPRX_MOUSE Nociceptin receptor (Orphanin FQ receptor) (Kappa-type 3 opioid receptor) (KOR-3) (ORGC) (K3 opiate receptor) - Mus musculus (Mouse).
OPRX_PIG Nociceptin receptor (Orphanin FQ receptor) (Kappa-type 3 opioid receptor) (KOR-3) (ORGC) (K3 opiate receptor) - Sus scrofa (Pig).
OPRX_RAT Nociceptin receptor (Orphanin FQ receptor) (Kappa-type 3 opioid receptor) (KOR-3) (ROR-C) (XOR1) - Rattus norvegicus (Rat).

O57585 OPIOID RECEPTOR HOMOLOGUE - BRACHYDANIO RERIO (ZEBRAFISH) (ZEBRA DANIO).

SSR1_HUMAN Somatostatin receptor type 1 (SS1R) (SRIF-2) - Homo sapiens (Human).
SSR1_MOUSE Somatostatin receptor type 1 (SS1R) (SRIF-2) - Mus musculus (Mouse).
SSR1_RAT Somatostatin receptor type 1 (SS1R) (SRIF-2) - Rattus norvegicus (Rat).
SSR2_BOVIN Somatostatin receptor type 2 (SS2R) (SRIF-1) - Bos taurus (Bovine).
SSR2_HUMAN Somatostatin receptor type 2 (SS2R) (SRIF-1) - Homo sapiens (Human).
SSR2_MOUSE Somatostatin receptor type 2 (SS2R) (SRIF-1) - Mus musculus (Mouse).
SSR2_PIG Somatostatin receptor type 2 (SS2R) (SRIF-1) - Sus scrofa (Pig).
SSR2_RAT Somatostatin receptor type 2 (SS2R) (SRIF-1) - Rattus norvegicus (Rat).

GPR8_HUMAN Probable G protein-coupled receptor GPR8 - Homo sapiens (Human).
SSR4_HUMAN Somatostatin receptor type 4 (SS4R) - Homo sapiens (Human).
SSR4_MOUSE Somatostatin receptor type 4 (SS4R) - Mus musculus (Mouse).
SSR4_RAT Somatostatin receptor type 4 (SS4R) - Rattus norvegicus (Rat).
SSR5_HUMAN Somatostatin receptor type 5 (SS5R) - Homo sapiens (Human).
SSR5_MOUSE Somatostatin receptor type 5 (SS5R) - Mus musculus (Mouse).
SSR5_RAT Somatostatin receptor type 5 (SS5R) - Rattus norvegicus (Rat).
SSRL_FUGRU Somatostatin-like receptor F_48D10.1 - Fugu rubripes (Japanese pufferfish) (Takifugu rubripes).
Scan History
OWL26_0    2  100  NSINGLE    
SPTR37_9f 2 50 NSINGLE
Initial Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
RYTKMKTATNIYIF OPRK_CAVPO 86 86 -
RYTKMKTATNIYIF OPRM_HUMAN 97 97 -
RYTKMKTATNIYIF OPRD_HUMAN 76 76 -
RHTKMKTATNIYIF OPRX_HUMAN 78 78 -

Motif 2 width=13
Element Seqn Id St Int Rpt
DYYNMFTSIFTLT OPRK_CAVPO 138 38 -
DYYNMFTSIFTLC OPRM_HUMAN 149 38 -
DYYNMFTSIFTLT OPRD_HUMAN 128 38 -
DYYNMFTSTFTLT OPRX_HUMAN 130 38 -

Motif 3 width=10
Element Seqn Id St Int Rpt
LDFRTPLKAK OPRK_CAVPO 167 16 -
LDFRTPRNAK OPRM_HUMAN 178 16 -
LDFRTPAKAK OPRD_HUMAN 157 16 -
LDVRTSSKAQ OPRX_HUMAN 159 16 -

Motif 4 width=13
Element Seqn Id St Int Rpt
LSGSREKDRNLRR OPRK_CAVPO 259 82 -
LSGSKEKDRNLRR OPRM_HUMAN 267 79 -
LSGSKEKDRSLRR OPRD_HUMAN 246 79 -
LSGSREKDRNLRR OPRX_HUMAN 248 79 -

Motif 5 width=8
Element Seqn Id St Int Rpt
FCIALGYT OPRK_CAVPO 314 42 -
FCIALGYT OPRM_HUMAN 322 42 -
LCIALGYA OPRD_HUMAN 302 43 -
FCTALGYV OPRX_HUMAN 303 42 -

Motif 6 width=10
Element Seqn Id St Int Rpt
FLDENFKRCF OPRK_CAVPO 332 10 -
FLDENFKRCF OPRM_HUMAN 340 10 -
FLDENFKRCF OPRD_HUMAN 320 10 -
FLDENFKACF OPRX_HUMAN 321 10 -
Final Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
RYTKMKTATNIYIF OPRK_RAT 86 86 -
RYTKMKTATNIYIF OPRK_MOUSE 86 86 -
RYTKMKTATNIYIF OPRK_HUMAN 86 86 -
RYTKMKTATNIYIF OPRK_CAVPO 86 86 -
RYTKMKTATNIYIF O42324 84 84 -
RYTKMKTATNIYIF OPRM_RAT 95 95 -
RYTKMKTATNIYIF OPRM_PIG 98 98 -
RYTKMKTATNIYIF OPRM_MOUSE 95 95 -
RYTKMKTATNIYIF OPRM_HUMAN 97 97 -
RYTKMKTATNIYIF OPRM_BOVIN 98 98 -
RYTKMKTATNIYIF OPRD_HUMAN 76 76 -
RYTKLKTATNIYIF OPRD_MOUSE 76 76 -
RYTKLKTATNIYIF OPRD_RAT 76 76 -
RHTKMKTATNIYIF OPRX_CAVPO 78 78 -
RHTKMKTATNIYIF OPRX_HUMAN 78 78 -
RHTKMKTATNIYIF OPRX_MOUSE 75 75 -
RHTKMKTATNIYIF OPRX_RAT 75 75 -
RHTKMKTATNIYIF OPRX_PIG 78 78 -

Motif 2 width=13
Element Seqn Id St Int Rpt
DYYNMFTSIFTLT OPRK_RAT 138 38 -
DYYNMFTSIFTLT OPRK_MOUSE 138 38 -
DYYNMFTSIFTLT OPRK_HUMAN 138 38 -
DYYNMFTSIFTLT OPRK_CAVPO 138 38 -
DYYNMFTSIFTLT O42324 136 38 -
DYYNMFTSIFTLC OPRM_RAT 147 38 -
DYYNMFTSIFTLC OPRM_PIG 150 38 -
DYYNMFTSIFTLC OPRM_MOUSE 147 38 -
DYYNMFTSIFTLC OPRM_HUMAN 149 38 -
DYYNMFTSIFTLC OPRM_BOVIN 150 38 -
DYYNMFTSIFTLT OPRD_HUMAN 128 38 -
DYYNMFTSIFTLT OPRD_MOUSE 128 38 -
DYYNMFTSIFTLT OPRD_RAT 128 38 -
DYYNMFTSTFTLT OPRX_CAVPO 130 38 -
DYYNMFTSTFTLT OPRX_HUMAN 130 38 -
DYYNMFTSTFTLT OPRX_MOUSE 127 38 -
DYYNMFTSTFTLT OPRX_RAT 127 38 -
DYYNMFTSAFTLT OPRX_PIG 130 38 -

Motif 3 width=10
Element Seqn Id St Int Rpt
LDFRTPLKAK OPRK_RAT 167 16 -
LDFRTPLKAK OPRK_MOUSE 167 16 -
LDFRTPLKAK OPRK_HUMAN 167 16 -
LDFRTPLKAK OPRK_CAVPO 167 16 -
LDFRTPRNAK O42324 165 16 -
LDFRTPRNAK OPRM_RAT 176 16 -
LDFRTPRNAK OPRM_PIG 179 16 -
LDFRTPRNAK OPRM_MOUSE 176 16 -
LDFRTPRNAK OPRM_HUMAN 178 16 -
LDLRTPRNAK OPRM_BOVIN 179 16 -
LDFRTPAKAK OPRD_HUMAN 157 16 -
LDFRTPAKAK OPRD_MOUSE 157 16 -
LDFRTPAKAK OPRD_RAT 157 16 -
LDVRTSSKAQ OPRX_CAVPO 159 16 -
LDVRTSSKAQ OPRX_HUMAN 159 16 -
LDVRTSSKAQ OPRX_MOUSE 156 16 -
LDVRTSSKAQ OPRX_RAT 156 16 -
LDVRTSSKAQ OPRX_PIG 159 16 -

Motif 4 width=13
Element Seqn Id St Int Rpt
LSGSREKDRNLRR OPRK_RAT 259 82 -
LSGSREKDRNLRR OPRK_MOUSE 259 82 -
LSGSREKDRNLRR OPRK_HUMAN 259 82 -
LSGSREKDRNLRR OPRK_CAVPO 259 82 -
LSGSKEKDRNLRR O42324 261 86 -
LSGSKEKDRNLRR OPRM_RAT 265 79 -
LSGSKEKDRNLRR OPRM_PIG 268 79 -
LSGSKEKDRNLRR OPRM_MOUSE 265 79 -
LSGSKEKDRNLRR OPRM_HUMAN 267 79 -
LSGSKEKDRNLRR OPRM_BOVIN 268 79 -
LSGSKEKDRSLRR OPRD_HUMAN 246 79 -
LSGSKEKDRSLRR OPRD_MOUSE 246 79 -
LSGSKEKDRSLRR OPRD_RAT 246 79 -
LSGSREKDRNLRR OPRX_CAVPO 248 79 -
LSGSREKDRNLRR OPRX_HUMAN 248 79 -
LSGSREKDRNLRR OPRX_MOUSE 245 79 -
LSGSREKDRNLRR OPRX_RAT 245 79 -
LSGSREKDRNLRR OPRX_PIG 248 79 -

Motif 5 width=8
Element Seqn Id St Int Rpt
FCIALGYT OPRK_RAT 314 42 -
FCIALGYT OPRK_MOUSE 314 42 -
FCIALGYT OPRK_HUMAN 314 42 -
FCIALGYT OPRK_CAVPO 314 42 -
FCIALGYT O42324 316 42 -
FCIALGYT OPRM_RAT 320 42 -
FCIALGYT OPRM_PIG 323 42 -
FCIALGYT OPRM_MOUSE 320 42 -
FCIALGYT OPRM_HUMAN 322 42 -
FCIALGYT OPRM_BOVIN 323 42 -
LCIALGYA OPRD_HUMAN 302 43 -
LCIALGYA OPRD_MOUSE 302 43 -
LCIALGYA OPRD_RAT 302 43 -
FCTALGYV OPRX_CAVPO 303 42 -
FCTALGYV OPRX_HUMAN 303 42 -
FCTALGYV OPRX_MOUSE 300 42 -
FCTALGYV OPRX_RAT 300 42 -
FCTALGYV OPRX_PIG 303 42 -

Motif 6 width=10
Element Seqn Id St Int Rpt
FLDENFKRCF OPRK_RAT 332 10 -
FLDENFKRCF OPRK_MOUSE 332 10 -
FLDENFKRCF OPRK_HUMAN 332 10 -
FLDENFKRCF OPRK_CAVPO 332 10 -
FLDENFKRCF O42324 334 10 -
FLDENFKRCF OPRM_RAT 338 10 -
FLDENFKRCF OPRM_PIG 341 10 -
FLDENFKRCF OPRM_MOUSE 338 10 -
FLDENFKRCF OPRM_HUMAN 340 10 -
FLDENFKRCF OPRM_BOVIN 341 10 -
FLDENFKRCF OPRD_HUMAN 320 10 -
FLDENFKRCF OPRD_MOUSE 320 10 -
FLDENFKRCF OPRD_RAT 320 10 -
FLDENFKACF OPRX_CAVPO 321 10 -
FLDENFKACF OPRX_HUMAN 321 10 -
FLDENFKACF OPRX_MOUSE 318 10 -
FLDENFKACF OPRX_RAT 318 10 -
FLDENFKACF OPRX_PIG 321 10 -