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PR00002

Identifier
GLABONE  [View Relations]  [View Alignment]  
Accession
PR00002
No. of Motifs
2
Creation Date
19-JUL-1991  (UPDATE 19-JUN-1999)
Title
Bone matrix GLA domain signature
Database References

PROSITE; PS00011 GLU_CARBOXYLATION
BLOCKS; BL00011
PFAM; PF00594 gla
INTERPRO; IPR002384
Literature References
1. PRICE, P.A., FRASER, J.D. AND METZ-VIRCA, G.
Molecular cloning of matrix Gla protein: Implications for substrate
recognition by the vitamin K-dependent gamma-carboxylase.
PROC.NATL.ACAD.SCI.U.S.A. 84 8335-8339 (1987).
 
2. PRICE, P.A., OTSUKA, A.S., POSER, J.W., KRISTAPONIS, J. AND RAMAN, N.
Characterization of a gamma-carboxyglutamic acid-containing protein 
from bone.
PROC.NATL.ACAD.SCI.U.S.A. 73 1447-1451 (1976).
 
3. PRICE, P.A. AND WILLIAMSON, M.K.
Primary structure of bovine matrix protein, a new vitamin K-dependent 
bone protein.
J.BIOL.CHEM. 260 14971-14975 (1985).
 
4. PAN, L.C., WILLIAMSON, M.K. AND PRICE, P.A.
Sequence of the precursor to rat bone gamma-carboxyglutamic acid protein 
that accumulates in warfarin-treated osteosarcoma cells.
J.BIOL.CHEM. 260 13398-13401 (1985).

Documentation
Bone gamma-carboxyglutamic acid protein (BGP) and matrix gamma- 
carboxyglutamic acid protein (MGP) are the only vitamin K-dependent 
proteins that have been isolated from bone. MGP is a 79 residue protein 
found in bone, dentin and cartilage: it contains 5 gamma-carboxyglutamic 
acid (Gla) residues (formed by post-translational modification of glutamic 
acid by vitamin K-dependent carboxylase) and a single disulphide bond. 
BGP is a 49 residue protein found in bone: it contains 3 Gla residues and 
a single disulphide bond. There is little similarity between these vitamin 
K-dependent bone proteins and the blood coagulation proteins, though they 
are believed to have diverged from a common ancestor [1].
 
GLABONE is a 2-element fingerprint that provides a signature for the 
invariant Gla domain of bone protein. The fingerprint was derived from an
initial alignment of 5 sequences (after Price et al. [1]): the motifs span
the full length of the Gla domain. Two iterations on OWL11.0 were required
to reach convergence, at which point a true set comprising 17 sequences
was identified.
 
An update on SPTR37_9f identified a true set of 22 sequences.
Summary Information
22 codes involving  2 elements
Composite Feature Index
22222
12
True Positives
MGP_BOVIN     MGP_GALGA     MGP_HUMAN     MGP_MOUSE     
MGP_RABIT MGP_RAT O42413 OSTC_BOVIN
OSTC_CANFA OSTC_CHICK OSTC_DRONO OSTC_FELCA
OSTC_HUMAN OSTC_LEPMA OSTC_MACFA OSTC_MOUSE
OSTC_RABIT OSTC_RAT OSTC_SPAAU OSTC_XENLA
OSTC_XIPGL OSTR_MOUSE
Sequence Titles
MGP_BOVIN   MATRIX GLA-PROTEIN PRECURSOR (MGP) - BOS TAURUS (BOVINE). 
MGP_GALGA MATRIX GLA-PROTEIN (MGP) - GALEORHINUS GALEUS (SOUPFIN SHARK).
MGP_HUMAN MATRIX GLA-PROTEIN PRECURSOR (MGP) - HOMO SAPIENS (HUMAN).
MGP_MOUSE MATRIX GLA-PROTEIN PRECURSOR (MGP) - MUS MUSCULUS (MOUSE).
MGP_RABIT MATRIX GLA-PROTEIN PRECURSOR (MGP) - ORYCTOLAGUS CUNICULUS (RABBIT).
MGP_RAT MATRIX GLA-PROTEIN PRECURSOR (MGP) - RATTUS NORVEGICUS (RAT).
O42413 MATRIX GLA-PROTEIN PRECURSOR (MGP) - GALLUS GALLUS (CHICKEN).
OSTC_BOVIN OSTEOCALCIN PRECURSOR (GAMMA-CARBOXYGLUTAMIC ACID-CONTAINING PROTEIN) (BONE GLA-PROTEIN) (BGP) - BOS TAURUS (BOVINE).
OSTC_CANFA OSTEOCALCIN (GAMMA-CARBOXYGLUTAMIC ACID-CONTAINING PROTEIN) (BONE GLA- PROTEIN) (BGP) - CANIS FAMILIARIS (DOG).
OSTC_CHICK OSTEOCALCIN PRECURSOR (GAMMA-CARBOXYGLUTAMIC ACID-CONTAINING PROTEIN) (BONE GLA-PROTEIN) (BGP) - GALLUS GALLUS (CHICKEN).
OSTC_DRONO OSTEOCALCIN (GAMMA-CARBOXYGLUTAMIC ACID-CONTAINING PROTEIN) (BONE GLA- PROTEIN) (BGP) - DROMAIUS NOVAE-HOLLANDIAE (EMU).
OSTC_FELCA OSTEOCALCIN (GAMMA-CARBOXYGLUTAMIC ACID-CONTAINING PROTEIN) (BONE GLA- PROTEIN) (BGP) - FELIS SILVESTRIS CATUS (CAT).
OSTC_HUMAN OSTEOCALCIN PRECURSOR (GAMMA-CARBOXYGLUTAMIC ACID-CONTAINING PROTEIN) (BONE GLA-PROTEIN) (BGP) - HOMO SAPIENS (HUMAN).
OSTC_LEPMA OSTEOCALCIN (GAMMA-CARBOXYGLUTAMIC ACID-CONTAINING PROTEIN) (BONE GLA- PROTEIN) (BGP) - LEPOMIS MACROCHIRUS (BLUEGILL).
OSTC_MACFA OSTEOCALCIN (GAMMA-CARBOXYGLUTAMIC ACID-CONTAINING PROTEIN) (BONE GLA- PROTEIN) (BGP) - MACACA FASCICULARIS (CRAB EATING MACAQUE) (CYNOMOLGUS MONKEY).
OSTC_MOUSE OSTEOCALCIN PRECURSOR (GAMMA-CARBOXYGLUTAMIC ACID-CONTAINING PROTEIN) (BONE GLA-PROTEIN) (BGP) - MUS MUSCULUS (MOUSE).
OSTC_RABIT OSTEOCALCIN (GAMMA-CARBOXYGLUTAMIC ACID-CONTAINING PROTEIN) (BONE GLA-PROTEIN) (BGP) - ORYCTOLAGUS CUNICULUS (RABBIT).
OSTC_RAT OSTEOCALCIN PRECURSOR (GAMMA-CARBOXYGLUTAMIC ACID-CONTAINING PROTEIN) (BONE GLA-PROTEIN) (BGP) - RATTUS NORVEGICUS (RAT).
OSTC_SPAAU OSTEOCALCIN (GAMMA-CARBOXYGLUTAMIC ACID-CONTAINING PROTEIN) (BONE GLA- PROTEIN) (BGP) - SPARUS AURATA (GILTHEAD SEA BREAM).
OSTC_XENLA OSTEOCALCIN (GAMMA-CARBOXYGLUTAMIC ACID-CONTAINING PROTEIN) (BONE GLA- PROTEIN) (BGP) - XENOPUS LAEVIS (AFRICAN CLAWED FROG).
OSTC_XIPGL OSTEOCALCIN (GAMMA-CARBOXYGLUTAMIC ACID-CONTAINING PROTEIN) (BONE GLA- PROTEIN) (BGP) - XIPHIAS GLADIUS (SWORDFISH).
OSTR_MOUSE OSTEOCALCIN-RELATED PROTEIN PRECURSOR (OC-X) (NEPHROCALCIN) - MUS MUSCULUS (MOUSE).
Scan History
OWL11_0    2  50   NSINGLE    
OWL17_1 1 50 NSINGLE
OWL18_0 1 50 NSINGLE
OWL19_1 1 50 NSINGLE
OWL26_0 1 200 NSINGLE
SPTR37_9f 2 100 NSINGLE
Initial Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
ELNREACDDFKLCERYA GEBOM 48 48 -
EPHREVCELNPNCDELA GERT 66 66 -
EPRREVCELNPDCDELA OSTC_HUMAN 68 68 -
EPTREQCELNPACDELS OSTC_MOUSE 62 62 -
ELNREACDDFKLCERYA MGP_BOVIN 67 67 -

Motif 2 width=11
Element Seqn Id St Int Rpt
YGLKTAYKRIY OSTC_MOUSE 81 2 -
YGYNAAYDRYF MGP_BOVIN 86 2 -
YGYNAAYDRYF GEBOM 67 2 -
IGFQDAYKRIY GERT 85 2 -
IGFQEAYRRFY OSTC_HUMAN 87 2 -
Final Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
EPKREVCELNPDCDELA OSTC_MACFA 17 17 -
EPKREVCELNPDCDELA OSTC_BOVIN 68 68 -
EPRREVCELNPDCDELA OSTC_HUMAN 68 68 -
ESQREVCELNPDCDELA OSTC_XENLA 17 17 -
EAQREVCELNPDCDELA OSTC_DRONO 16 16 -
EPKREVCELNPNCDELA OSTC_CANFA 17 17 -
EPKREICELNPDCDELA OSTC_FELCA 17 17 -
EAQREVCELSPDCDELA OSTC_CHICK 65 65 -
EPHREVCELNPNCDELA OSTC_RAT 66 66 -
EPKREVCELNPDCDELA OSTC_RABIT 17 17 -
EPTREQCELNPACDELS OSTC_MOUSE 62 62 -
EPTRELCELDPACDELS OSTR_MOUSE 62 62 -
ESLREVCELNVSCDEMA OSTC_XIPGL 13 13 -
ELNREACDDYKLCERYA MGP_RABIT 67 67 -
EINREACDDYKLCERYA MGP_MOUSE 67 67 -
EINREACDDYKLCERYA MGP_RAT 67 67 -
ELNREACDDYRLCERYA MGP_HUMAN 67 67 -
ELNREACDDFKLCERYA MGP_BOVIN 67 67 -
ESLREVCELNLACEHMM OSTC_SPAAU 11 11 -
ESLREVCEANLACEDMM OSTC_LEPMA 11 11 -
ERQREICEDFHLCEQYA O42413 68 68 -
ERTREKCEEYRPCERLA MGP_GALGA 43 43 -

Motif 2 width=11
Element Seqn Id St Int Rpt
IGFQEAYRRFY OSTC_MACFA 36 2 -
IGFQEAYRRFY OSTC_BOVIN 87 2 -
IGFQEAYRRFY OSTC_HUMAN 87 2 -
IGFQEAYRRFY OSTC_XENLA 36 2 -
IGFQEAYRRFY OSTC_DRONO 35 2 -
IGFQEAYQRFY OSTC_CANFA 36 2 -
IGFQDAYRRFY OSTC_FELCA 36 2 -
IGFQEAYRRFY OSTC_CHICK 84 2 -
IGFQDAYKRIY OSTC_RAT 85 2 -
VGLQDAYQRFY OSTC_RABIT 36 2 -
YGLKTAYKRIY OSTC_MOUSE 81 2 -
YGLKTAYRRIY OSTR_MOUSE 81 2 -
AGIVAAYIAYY OSTC_XIPGL 32 2 -
YGYNAAYNRYF MGP_RABIT 86 2 -
YGYNAAYNRYF MGP_MOUSE 86 2 -
YGYNAAYNRYF MGP_RAT 86 2 -
YGYNAAYNRYF MGP_HUMAN 86 2 -
YGYNAAYDRYF MGP_BOVIN 86 2 -
EGIIAAYTAYY OSTC_SPAAU 30 2 -
QGIIAAYTAYY OSTC_LEPMA 30 2 -
HGYPAAYRHYF O42413 87 2 -
VGLKRAYGKYF MGP_GALGA 62 2 -