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PR01993

Identifier
GPR162  [View Relations]  [View Alignment]  
Accession
PR01993
No. of Motifs
10
Creation Date
02-MAR-2009
Title
GP162 7TM protein signature
Database References
PRINTS; PR01991 GPR153GPR162
PROSITE; PS00237 G_PROTEIN_RECEP_F1_1; PS50262 G_PROTEIN_RECEP_F1_2
PFAM; PF00001 7tm_1
INTERPRO; IPR000276
Literature References
1. ANSARI-LARI, M.A., OELTJEN, J.C., SCHWARTZ, S., ZHANG, Z., MUZNY, D.M.,
LU, J., GORRELL, J.H., CHINAULT, A.C., BELMONT, J.W., MILLER, W. AND
GIBBS, R.A.
Comparative sequence analysis of a gene-rich cluster at human chromosome
12p13 and its syntenic region in mouse chromosome 6.
GENOME RES. 8 29-40 (1998).
 
2. ANSARI-LARI, M.A., SHEN, Y., MUZNY, D.M., LEE, W. AND GIBBS, R.A.
Large-scale sequencing in human chromosome 12p13: experimental and
computational gene structure determination.
GENOME RES. 7 268-280 (1997).
 
3. ANSARI-LARI, M.A., MUZNY, D.M., LU, J., LU, F., LILLEY, C.E., SPANOS, S.,
MALLEY, T. AND GIBBS, R.A.
A gene-rich cluster between the CD4 and triosephosphate isomerase genes at
human chromosome 12p13.
GENOME RES. 6 314-326 (1996)
 
4. VASSILATIS, D.K., HOHMANN, J.G., ZENG, H., LI, F., RANCHALIS, J.E.,
MORTRUD, M.T., BROWN, A., RODRIGUEZ, S.S., WELLER, J.R., WRIGHT, A.C.,
BERGMANN, J.E. AND GAITANARIS, G.A.
The G protein-coupled receptor repertoires of human and mouse.
PROC.NATL.ACAD.SCI.U.S.A. 100 4903-4908 (2003).

Documentation
Computational methods, including percent identity plots, hydropathy profiles
and BLAST, have been used to analyse a gene-rich cluster at human chromosome
12p13 and to compare it with its syntenic region in mouse chromosome 6 [1-3].
Of 6 genes identified, one of these, termed Gene A, revealed 3 splice forms:
form A2 demonstrated a clear 7-transmembrane (TM) hydropathy profile, and
showed very weak similarity to members of the G protein-coupled receptor 
(GPCR) superfamily [3].
 
GPCRs mediate a wide range of physiological functions, are major drug
targets, and are consequently the subject of considerable research interest.
In 2003, Vassilatis et al. reported that the repertoire of GPCRs for 
endogenous ligands consisted of 367 receptors in humans and 392 in mice,
including 26 from human and 83 from mouse that had not been identified
previously [4]. 
 
Of these novel receptors, GP162 (also known as GRCA) does show some
similarity to rhodopsin-class GPCRs (most notably to certain tyramine and
serotonin receptors, particularly in the region of TM domain 4), but the
overall degree of similarity is very low, and evidence that it is functional
GPCR has not been gathered experimentally.
 
GPR162 is a 10-element fingerprint that provides a signature for the 
GP162 7TM protein. The fingerprint was derived from an initial alignment of
3 sequences - the motifs were drawn from conserved regions spanning
virtually the full alignment length, focusing on those sections that 
characterise GP162 but distinguish it from closely related GP153 proteins.
A single iteration on SPTR55_38f was required to reach convergence, no
further sequences being identified beyond the starting set. A single partial
match was also found, Q3TY71_MOUSE, a murine visual cortex protein that
matches motifs 1-3.
Summary Information
   3 codes involving 10 elements
0 codes involving 9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
1 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
103333333333
90000000000
80000000000
70000000000
60000000000
50000000000
40000000000
31110000000
20000000000
12345678910
True Positives
GP162_HUMAN   GP162_MOUSE   Q0VD40_BOVIN  
True Positive Partials
Codes involving 3 elements
Q3TY71_MOUSE
Sequence Titles
GP162_HUMAN Probable G-protein coupled receptor 162 (Gene-rich cluster gene A protein) - Homo sapiens (Human). 
GP162_MOUSE Probable G-protein coupled receptor 162 - Mus musculus (Mouse).
Q0VD40_BOVIN Similar to G protein-coupled receptor 162 - Bos taurus (Bovine).

Q3TY71_MOUSE Visual cortex cDNA, RIKEN full-length enriched library, clone:K430345D13 product:hypothetical Rhodopsin-like GPCR superfamily containing protein, full insert sequence - Mus musculus (Mouse).
Scan History
SPTR55_38f 1  100  NSINGLE    
Initial Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
MARGGAGAEEASLRSNALS GP162_HUMAN 1 1 -
MARGGLGAEEASLRSNALS GP162_MOUSE 1 1 -
MARGGAGAEEASLRSNALS Q0VD40_BOVIN 1 1 -

Motif 2 width=21
Element Seqn Id St Int Rpt
TFYQTLWARPRRARQARRVGG GP162_HUMAN 201 181 -
TFYQTLWARPRRARQARRAGG GP162_MOUSE 201 181 -
TFYQTLWARPRRARQARRAGG Q0VD40_BOVIN 201 181 -

Motif 3 width=18
Element Seqn Id St Int Rpt
GTKAGGPGALGTRPAFEV GP162_HUMAN 224 2 -
GTKAGGLGGLGTRPAFEV GP162_MOUSE 224 2 -
GARGGGPGGLGTRPAFEV Q0VD40_BOVIN 224 2 -

Motif 4 width=22
Element Seqn Id St Int Rpt
MSEEDGDDDGGCDDYAEGRVCK GP162_HUMAN 345 103 -
MSEDDGDDDGTCDDYTDGRVCK GP162_MOUSE 345 103 -
MSEEDGEDDEGCDDYADGRVCK Q0VD40_BOVIN 345 103 -

Motif 5 width=28
Element Seqn Id St Int Rpt
RFDANGATGPGSRDPAQVKLLPGRHMLF GP162_HUMAN 368 1 -
RFDANGATGSGSRDPTQVKLLPGRHMLF GP162_MOUSE 368 1 -
RFDANGATGPGGRDPTQVKLLPGRHMLF Q0VD40_BOVIN 368 1 -

Motif 6 width=16
Element Seqn Id St Int Rpt
NIFSTPREPGSFLHKW GP162_HUMAN 418 22 -
NIFSTPRAPGSFLHKW GP162_MOUSE 418 22 -
NIFSTPRAPGSFLHKW Q0VD40_BOVIN 418 22 -

Motif 7 width=18
Element Seqn Id St Int Rpt
RVLPAQSRALGGPPEYLG GP162_HUMAN 440 6 -
RILPAQSRALGGPPEYLG GP162_MOUSE 440 6 -
RVLPAQSRALGGPPEHLG Q0VD40_BOVIN 440 6 -

Motif 8 width=19
Element Seqn Id St Int Rpt
GGLASLRQFLESGVLGSGG GP162_HUMAN 474 16 -
GGLASLRQFLESGVLGSGG GP162_MOUSE 474 16 -
GGLATLRQFLEGGVLGSGG Q0VD40_BOVIN 474 16 -

Motif 9 width=23
Element Seqn Id St Int Rpt
SLGSPESRAVGLPLGLSAGRRCS GP162_HUMAN 539 46 -
SLGSPDSRAVGLPLGLSAGRRCS GP162_MOUSE 539 46 -
SLGSPDSRAIGLPLGLSAGRRCS Q0VD40_BOVIN 539 46 -

Motif 10 width=20
Element Seqn Id St Int Rpt
SARAWGGSWGPGNPIFPQLT GP162_HUMAN 568 6 -
SSRAWGRPWGPGNPIFPQLT GP162_MOUSE 568 6 -
STKAWGGSWGPGNPIFPQLT Q0VD40_BOVIN 568 6 -
Final Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
MARGGAGAEEASLRSNALS GP162_HUMAN 1 1 -
MARGGLGAEEASLRSNALS GP162_MOUSE 1 1 -
MARGGAGAEEASLRSNALS Q0VD40_BOVIN 1 1 -

Motif 2 width=21
Element Seqn Id St Int Rpt
TFYQTLWARPRRARQARRVGG GP162_HUMAN 201 181 -
TFYQTLWARPRRARQARRAGG GP162_MOUSE 201 181 -
TFYQTLWARPRRARQARRAGG Q0VD40_BOVIN 201 181 -

Motif 3 width=18
Element Seqn Id St Int Rpt
GTKAGGPGALGTRPAFEV GP162_HUMAN 224 2 -
GTKAGGLGGLGTRPAFEV GP162_MOUSE 224 2 -
GARGGGPGGLGTRPAFEV Q0VD40_BOVIN 224 2 -

Motif 4 width=22
Element Seqn Id St Int Rpt
MSEEDGDDDGGCDDYAEGRVCK GP162_HUMAN 345 103 -
MSEDDGDDDGTCDDYTDGRVCK GP162_MOUSE 345 103 -
MSEEDGEDDEGCDDYADGRVCK Q0VD40_BOVIN 345 103 -

Motif 5 width=28
Element Seqn Id St Int Rpt
RFDANGATGPGSRDPAQVKLLPGRHMLF GP162_HUMAN 368 1 -
RFDANGATGSGSRDPTQVKLLPGRHMLF GP162_MOUSE 368 1 -
RFDANGATGPGGRDPTQVKLLPGRHMLF Q0VD40_BOVIN 368 1 -

Motif 6 width=16
Element Seqn Id St Int Rpt
NIFSTPREPGSFLHKW GP162_HUMAN 418 22 -
NIFSTPRAPGSFLHKW GP162_MOUSE 418 22 -
NIFSTPRAPGSFLHKW Q0VD40_BOVIN 418 22 -

Motif 7 width=18
Element Seqn Id St Int Rpt
RVLPAQSRALGGPPEYLG GP162_HUMAN 440 6 -
RILPAQSRALGGPPEYLG GP162_MOUSE 440 6 -
RVLPAQSRALGGPPEHLG Q0VD40_BOVIN 440 6 -

Motif 8 width=19
Element Seqn Id St Int Rpt
GGLASLRQFLESGVLGSGG GP162_HUMAN 474 16 -
GGLASLRQFLESGVLGSGG GP162_MOUSE 474 16 -
GGLATLRQFLEGGVLGSGG Q0VD40_BOVIN 474 16 -

Motif 9 width=23
Element Seqn Id St Int Rpt
SLGSPESRAVGLPLGLSAGRRCS GP162_HUMAN 539 46 -
SLGSPDSRAVGLPLGLSAGRRCS GP162_MOUSE 539 46 -
SLGSPDSRAIGLPLGLSAGRRCS Q0VD40_BOVIN 539 46 -

Motif 10 width=20
Element Seqn Id St Int Rpt
SARAWGGSWGPGNPIFPQLT GP162_HUMAN 568 6 -
SSRAWGRPWGPGNPIFPQLT GP162_MOUSE 568 6 -
STKAWGGSWGPGNPIFPQLT Q0VD40_BOVIN 568 6 -