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PR00180

Identifier
CRETINALDHBP  [View Relations]  [View Alignment]  
Accession
PR00180
No. of Motifs
5
Creation Date
28-FEB-1995  (UPDATE 04-JUL-1999)
Title
Cellular retinaldehyde-binding protein signature
Database References

PFAM; PF00650 CRAL_TRIO
INTERPRO; IPR001071
Literature References
1. CRABB, J.W., JOHNSON, C.M., CARR, S.A., ARMES, L.G. AND SAARI, J.C.
The complete primary structure of the cellular retinaldehyde-binding
protein from bovine retina.
J.BIOL.CHEM. 263 18678-18687 (1988).
 
2. CRABB, J.W., JOHNSON, C.M., CARR, S.A., ARMES, L.G. AND SAARI, J.C.
Cloning of the cDNAs encoding the cellular retinaldehyde-binding protein
from bovine and human retina and comparison of the protein structures.
J.BIOL.CHEM. 263 18688-18692 (1988).
 
3. SATO, Y., ARAI, H., MIYATA, A., TOKITA, S., YAMAMOTO, K., TANABE, T.
AND INOUE, K.
Primary structure of alpha-tocopherol transfer protein from rat liver. 
Homology with cellular retinaldehyde-binding protein.
J.BIOL.CHEM. 268 17705-17710 (1993). 

Documentation
Cellular retinaldehyde-binding protein (CRALBP) carries 11-cis-retinol and
11-cis-retinaldehyde as endogenous ligands: it is found only in visual 
tissue, where it serves as a stereo-selective agent and/or a substrate
carrier protein [1]. CRALBP can protect its substrate from photo-isomeris-
ation, suggesting a possible role in the generation of 11-cis-retinols in
the visual cycle [2]. Sequence studies have revealed no similarities either
to other lipophile-binding proteins (such as fatty-acid binding proteins)
or to other retinoid-binding proteins (such as rhodopsin or cone visual
pigments) [1]. The location of the binding site and exact function of
CRALBP are still unclear [1,2].
 
Alpha-tocopherol transport protein is known to share sequence similarity
with CRALBP [3]. This protein binds only alpha-tocopherol (and not the
gamma form), and as such displays the same sort of stereo-selective
binding as implicated with CRALBP [3]. 
 
CRETINALDHBP is a 5-element fingerprint that provides a signature for
cellular retinaldehyde binding and alpha-tocopherol transport proteins.
The fingerprint was derived from an initial alignment of 3 sequences:
the motifs were drawn from conserved regions spanning virtually the full
alignment length. A single iteration on OWL25.1 was required to reach
convergence, no further sequences being identified beyond the starting set.
 
An update on SPTR37_9f identified a true set of 4 sequences, and 3
partial matches.
Summary Information
   4 codes involving  5 elements
0 codes involving 4 elements
0 codes involving 3 elements
3 codes involving 2 elements
Composite Feature Index
544444
400000
300000
230030
12345
True Positives
CRAL_BOVIN    CRAL_HUMAN    TTPA_HUMAN    TTPA_RAT      
True Positive Partials
Codes involving 2 elements
O76054 SC14_YARLI YKJ1_YEAST
Sequence Titles
CRAL_BOVIN  CELLULAR RETINALDEHYDE-BINDING PROTEIN (CRALBP) - BOS TAURUS (BOVINE). 
CRAL_HUMAN CELLULAR RETINALDEHYDE-BINDING PROTEIN (CRALBP) - HOMO SAPIENS (HUMAN).
TTPA_HUMAN ALPHA-TOCOPHEROL TRANSFER PROTEIN (ALPHA-TTP) - HOMO SAPIENS (HUMAN).
TTPA_RAT ALPHA-TOCOPHEROL TRANSFER PROTEIN (ALPHA-TTP) - RATTUS NORVEGICUS (RAT).

O76054 WUGSC:H_DJ130H16.5 PROTEIN - HOMO SAPIENS (HUMAN).
SC14_YARLI SEC14 CYTOSOLIC FACTOR (PHOSPHATIDYLINOSITOL/PHOSPHATIDYLCHOLINE TRANSFER PROTEIN) (PI/PC TP) - YARROWIA LIPOLYTICA (CANDIDA LIPOLYTICA).
YKJ1_YEAST 36.1 KD PROTEIN IN BUD2-MIF2 INTERGENIC REGION - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
Scan History
OWL25_1    1  100  NSINGLE    
SPTR37_9f 2 26 NSINGLE
Initial Motifs
Motif 1  width=23
Element Seqn Id St Int Rpt
FFLRFIRARKFNVGRAYELLRGY CRAL_HUMAN 94 94 -
FFLRFIRARKFHVGRAYELLRGY CRAL_BOVIN 94 94 -
FLLRFLRARDFDLDLAWRLMKNY A47404 51 51 -

Motif 2 width=25
Element Seqn Id St Int Rpt
AGYPGVLSTRDKYGRVVMLFIIENW CRAL_BOVIN 141 24 -
AGYHGVLRSRDPTGSRVLIYRISYW A47404 98 24 -
AGYPGVLSSRDKYGRVVMLFNIENW CRAL_HUMAN 141 24 -

Motif 3 width=22
Element Seqn Id St Int Rpt
SDLRKMVDMLQDSFPARFKAIH CRAL_BOVIN 217 51 -
SVAKKIAAVVTDSFPLKVRGIH A47404 174 51 -
SDLRKMVDMLQDSFPARFKAIH CRAL_HUMAN 217 51 -

Motif 4 width=20
Element Seqn Id St Int Rpt
FSMIKPFLTEKIKGRIHLHG A47404 207 11 -
YNVVKPFLKSKLLQRVFVHG CRAL_BOVIN 250 11 -
YNVVKPFLKSKLLERVFVHG CRAL_HUMAN 250 11 -

Motif 5 width=10
Element Seqn Id St Int Rpt
DILPLEYGGN A47404 239 12 -
NILPSDFGGT CRAL_HUMAN 282 12 -
DILPSDFGGT CRAL_BOVIN 282 12 -
Final Motifs
Motif 1  width=23
Element Seqn Id St Int Rpt
FFLRFIRARKFHVGRAYELLRGY CRAL_BOVIN 94 94 -
FLLRFLRARDFDLDLAWRLLKNY TTPA_HUMAN 51 51 -
FFLRFIRARKFNVGRAYELLRGY CRAL_HUMAN 94 94 -
FLLRFLRARDFDLDLAWRLMKNY TTPA_RAT 51 51 -

Motif 2 width=25
Element Seqn Id St Int Rpt
AGYPGVLSTRDKYGRVVMLFIIENW CRAL_BOVIN 141 24 -
AGYHGVLRSRDPTGSKVLIYRIAHW TTPA_HUMAN 98 24 -
AGYPGVLSSRDKYGRVVMLFNIENW CRAL_HUMAN 141 24 -
AGYHGVLRSRDPTGSRVLIYRISYW TTPA_RAT 98 24 -

Motif 3 width=22
Element Seqn Id St Int Rpt
SDLRKMVDMLQDSFPARFKAIH CRAL_BOVIN 217 51 -
SVAKKIAAVLTDSFPLKVRGIH TTPA_HUMAN 174 51 -
SDLRKMVDMLQDSFPARFKAIH CRAL_HUMAN 217 51 -
SVAKKIAAVVTDSFPLKVRGIH TTPA_RAT 174 51 -

Motif 4 width=20
Element Seqn Id St Int Rpt
YNVVKPFLKSKLLQRVFVHG CRAL_BOVIN 250 11 -
FSMIKPFLTEKIKERIHMHG TTPA_HUMAN 207 11 -
YNVVKPFLKSKLLERVFVHG CRAL_HUMAN 250 11 -
FSMIKPFLTEKIKGRIHLHG TTPA_RAT 207 11 -

Motif 5 width=10
Element Seqn Id St Int Rpt
DILPSDFGGT CRAL_BOVIN 282 12 -
DILPLEYGGE TTPA_HUMAN 239 12 -
NILPSDFGGT CRAL_HUMAN 282 12 -
DILPLEYGGN TTPA_RAT 239 12 -