Hyperlinks have been added to sections of the standard BLAST output so that the
results may be more easily examined. At the top of the output an additional summary
of detected fingerprints has been included. The three main parts to the
BLAST output are,
i) fingerprint summary - this displays in tabular form, upto 10 of the most frequently detected fingerprints in the BLAST summary. Hyperlinks to the fingerprint entry and graphScan are included. GraphScan help is availbale here.
ii) blast summary - displays the first 60 characters of the FASTA sequence description line, followed by the score of the highest scoring HSP (high scoring segment pair) , the lowest P-value (probablity that match is by chance) of a given HSP set, and finally the number of HSPs which give the lowest P-values. The description line consists of the PRINTS ID name (1), PRINTS accession number (2), motifs in the protein (3) / total motifs in fingerprint (4), sequence ID name (5), sequence accession number (6) and protein description (7).
PRINTS ID Code|Occur | Picture --------------+------+----------- CALPAIN | 25 | GraphScan
Three hyperlinks are present in each description line, the PRINTS (1) and protein (5) ID codes are linked to the appropriate database entries, and the number of matching motifs (3/4) are linked to a graphical representation of the fingerprint with both the given sequence and a verified family member. A labelled summary section is shown below,
iii) blast alignments - includes the full sequence description line, alignments and statistical information of all HSPs for each sequence.
Smallest Sum High Probability Sequences producing High-scoring Segment Pairs: Score P(N) N 1 | 2 |3/4| 5 | 6 | 7 PRINTS:CALPAIN PR00704 9/9 CAN1_HUMAN P07384 CALPAIN 1, L... 3762 0.0 1 PRINTS:CALPAIN PR00704 9/9 AF021847 AF021847 Mus musculus... 2196 0.0 2 PRINTS:CALPAIN PR00704 9/9 RNU53858 U53858 Rattus norvegi... 2191 0.0 2
More detailed information about BLAST including, descriptions of the BLAST search parameters, a BLAST manual and references for BLAST and its statistics, can be found on the NCBI BLAST web pages.
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