WORKLIST ENTRIES (1):

LYSOZYMEG View alignment        Lysozyme G signature
 Type of fingerprint: COMPOUND with 8  elements
Links:
   PRINTS; PR00131 GLHYDRLASE1; PR00132 GLHYDRLASE2; PR00133 GLHYDRLASE3
   PRINTS; PR00732 GLHYDRLASE4; PR00733 GLHYDRLASE6; PR00734 GLHYDRLASE7
   PRINTS; PR00735 GLHYDRLASE8; PR00134 GLHYDRLASE10; PR00911 GLHYDRLASE11
   PRINTS; PR00736 GLHYDRLASE15; PR00737 GLHYDRLASE16; PR00738 GLHYDRLASE20
   PRINTS; PR00739 GLHYDRLASE26; PR00740 GLHYDRLASE27; PR00741 GLHYDRLASE29
   PRINTS; PR00843 GLHYDRLASE30; PR00742 GLHYDRLASE35; PR00743 GLHYDRLASE36
   PRINTS; PR00744 GLHYDRLASE37; PR00745 GLHYDRLASE39; PR00746 GLHYDRLASE41
   PRINTS; PR00747 GLHYDRLASE47; PR00844 GLHYDRLASE48; PR00845 GLHYDRLASE52
   PRINTS; PR00846 GLHYDRLASE56; PR00849 GLHYDRLASE58; PR00850 GLHYDRLASE59
   PRINTS; PR00748 MELIBIASE; PR00135 LYZLACT; PR00137 LYSOZYME
   PRINTS; PR00684 T4LYSOZYME; PR00110 ALPHAAMYLASE; PR00750 BETAAMYLASE
   INTERPRO; IPR002152
   PDB; 153L 3Dinfo
   SCOP; 153L
   CATH; 153L

 Creation date 04-JUN-1997; UPDATE 07-JUN-1999

   1. WEAVER, L.H., GRUTTER, M.G. AND MATTHEWS, B.W.
   The refined structures of goose lysozyme and its complex with a bound
   trisaccharide show that the "goose-type" lysozymes lack a catalytic
   aspartate residue. 
   J.MOL.BIOL. 245(1) 54-68 (1995).

   2. HENRISSAT, B. AND BAIROCH, A.
   Updating the sequence-based classification of glycosyl hydrolases.
   BIOCHEM.J. 316 695-696 (1996).

   Goose-type lysozyme (lysozyme G) hydrolyses 1,4-beta-linkages between
   N-acetyl-D-glucosamine and N-acetylmuramic acid in peptidoglycan hetero-
   polymers of prokaryote cell walls. Lysozyme G shows preference for 
   N-acetylmuramic acid residues that are substituted with a peptide moiety;
   it acts only as a glycanohydrolase.
  
   The structure of goose egg-white lysozyme (GEWL) with a bound trisaccharide
   has been refined to 1.6A resolution [1]. The trisaccharide occupies
   analogous sites to the B, C and D subsites of chicken (HEWL) and phage T4
   (T4L) lysozymes. All of these enzymes display the same characteristic 
   hydrogen bonding pattern between protein and substrate [1]. Glu73 of GEWL 
   corresponds closely to Glu35 of HEWL (Glu11 of T4L), supporting the view
   that this group is critical to the catalytic mechanism. However, lysozyme G
   has no obvious counterpart to Asp52 of chicken lysozyme (Asp20 in T4L),
   suggesting that a second acidic residue is not essential for its catalytic
   activity, and may not be required for the activity of other lysozymes.
  
   The structure of GEWL belongs to the mainly alpha class, its sequence
   showing no discernible similarity to other lysozymes. The enzyme has
   been classified as belonging to family 23 of glycosyl hydrolases [2]
   (http://expasy.hcuge.ch/cgi-bin/lists?glycosid.txt).
  
   LYSOZYMEG is an 8-element fingerprint that provides a signature for
   lysozyme G. The fingerprint was derived from an initial alignment of 4
   sequences: the motifs were drawn from conserved regions spanning the
   full alignment length - motif 1 spans the first 3 helices; motif 2 spans
   helices 4 and 5; motif 3 encompasses part of helices 6 and 7; motif 4 spans
   the C-terminus of helix 7 and the first beta-strand; motif 5 includes the
   second beta-strand; motif 6 encompasses part of helices 8 and 9; motif 7
   spans the C-terminus of helix 9 and helix 10; and motif 8 spans helices 11
   and 12. A single iteration on OWL29.3 was required to reach convergence,
   no further sequences being identified beyond the starting set. 
  
   An update on SPTR37_9f identified a true set of 4 sequences.

  SUMMARY INFORMATION
      4 codes involving  8 elements
      0 codes involving  7 elements
      0 codes involving  6 elements
      0 codes involving  5 elements
      0 codes involving  4 elements
      0 codes involving  3 elements
      0 codes involving  2 elements

   COMPOSITE FINGERPRINT INDEX
  
    8|   4    4    4    4    4    4    4    4  
    7|   0    0    0    0    0    0    0    0  
    6|   0    0    0    0    0    0    0    0  
    5|   0    0    0    0    0    0    0    0  
    4|   0    0    0    0    0    0    0    0  
    3|   0    0    0    0    0    0    0    0  
    2|   0    0    0    0    0    0    0    0  
   --+-----------------------------------------
     |   1    2    3    4    5    6    7    8  

True positives..
 LYG_ANSAN      LYG_CYGAT      LYG_STRCA      LYG_CHICK      


  PROTEIN TITLES
   LYG_ANSAN        LYSOZYME G (EC 3.2.1.17) (1,4-BETA-N-ACETYLMURAMIDASE) (GOOS
   LYG_CYGAT        LYSOZYME G (EC 3.2.1.17) (1,4-BETA-N-ACETYLMURAMIDASE) (GOOS
   LYG_STRCA        LYSOZYME G (EC 3.2.1.17) (1,4-BETA-N-ACETYLMURAMIDASE) (GOOS
   LYG_CHICK        LYSOZYME G PRECURSOR (EC 3.2.1.17) (1,4-BETA-N-ACETYLMURAMID

SCAN HISTORY OWL29_3 1 25 NSINGLE SPTR37_9f 2 6 NSINGLE INITIAL MOTIF SETS LYSOZYMEG1 Length of motif = 21 Motif number = 1 Lysozyme G motif I - 1 PCODE ST INT CYGNVNRIDTTGASCKTAKPE LYG_ANSAN 4 4 CYGNVNRIDTTGASCKTAKPE LYG_CYGAT 4 4 CYGSVSRIDTTGASCRTAKPE LYG_CHICK 30 30 CYGDVNRVDTTGASCKSAKPE LYG_STRCA 4 4 LYSOZYMEG2 Length of motif = 22 Motif number = 2 Lysozyme G motif II - 1 PCODE ST INT YCGVSASKKIAERDLQAMDRYK LYG_ANSAN 28 3 YCGVPASKTIAERDLKAMDRYK LYG_CYGAT 28 3 YCGVRASRTIAERDLGSMNKYK LYG_CHICK 54 3 YCGVAASRKIAERDLQSMDRYK LYG_STRCA 28 3 LYSOZYMEG3 Length of motif = 19 Motif number = 3 Lysozyme G motif III - 1 PCODE ST INT IKKVGEKLCVEPAVIAGII LYG_ANSAN 52 2 IKKVGEKLCVEPAVIAGII LYG_CYGAT 52 2 IKRVGEALCIEPAVIAGII LYG_CHICK 78 2 IKKVGQKLCVDPAVIAGII LYG_STRCA 52 2 LYSOZYMEG4 Length of motif = 21 Motif number = 4 Lysozyme G motif IV - 1 PCODE ST INT SRESHAGKVLKNGWGDRGNGF LYG_ANSAN 71 0 SRESHAGKVLKNGWGDRGNGF LYG_CYGAT 71 0 SRESHAGKILKNGWGDRGNGF LYG_CHICK 97 0 SRESHAGKALRNGWGDNGNGF LYG_STRCA 71 0 LYSOZYMEG5 Length of motif = 19 Motif number = 5 Lysozyme G motif V - 1 PCODE ST INT GLMQVDKRSHKPQGTWNGE LYG_ANSAN 92 0 GLMQVDKRSHKPQGTWNGE LYG_CYGAT 92 0 GLMQVDKRYHKIEGTWNGE LYG_CHICK 118 0 GLMQVDRRSHKPVGEWNGE LYG_STRCA 92 0 LYSOZYMEG6 Length of motif = 17 Motif number = 6 Lysozyme G motif VI - 1 PCODE ST INT KTIQKKFPSWTKDQQLK LYG_ANSAN 125 14 KRIQKKFPSWTKDQQLK LYG_CYGAT 125 14 KKIQRKFPRWTRDQQLK LYG_CHICK 151 14 KAIQKKFPRWTKEQQLK LYG_STRCA 125 14 LYSOZYMEG7 Length of motif = 22 Motif number = 7 Lysozyme G motif VII - 1 PCODE ST INT GGISAYNAGAGNVRSYARMDIG LYG_ANSAN 142 0 GGISAYNAGAGNVRSYARMDIG LYG_CYGAT 142 0 GGISAYNAGVGNVRSYERMDIG LYG_CHICK 168 0 GGISAYNAGPGNVRSYERMDIG LYG_STRCA 142 0 LYSOZYMEG8 Length of motif = 21 Motif number = 8 Lysozyme G motif VIII - 1 PCODE ST INT TTHDDYANDVVARAQYYKQHG LYG_ANSAN 164 0 TTHDDYANDVVARAQYYKQHG LYG_CYGAT 164 0 TLHDDYSNDVVARAQYFKQHG LYG_CHICK 190 0 TTHDDYANDVVARAQYYKQHG LYG_STRCA 164 0 FINAL MOTIF SETS LYSOZYMEG1 Length of motif = 21 Motif number = 1 Lysozyme G motif I - 2 PCODE ST INT CYGNVNRIDTTGASCKTAKPE LYG_ANSAN 4 4 CYGNVNRIDTTGASCKTAKPE LYG_CYGAT 4 4 CYGDVNRVDTTGASCKSAKPE LYG_STRCA 4 4 CYGSVSRIDTTGASCRTAKPE LYG_CHICK 30 30 LYSOZYMEG2 Length of motif = 22 Motif number = 2 Lysozyme G motif II - 2 PCODE ST INT YCGVSASKKIAERDLQAMDRYK LYG_ANSAN 28 3 YCGVPASKTIAERDLKAMDRYK LYG_CYGAT 28 3 YCGVAASRKIAERDLQSMDRYK LYG_STRCA 28 3 YCGVRASRTIAERDLGSMNKYK LYG_CHICK 54 3 LYSOZYMEG3 Length of motif = 19 Motif number = 3 Lysozyme G motif III - 2 PCODE ST INT IKKVGEKLCVEPAVIAGII LYG_ANSAN 52 2 IKKVGEKLCVEPAVIAGII LYG_CYGAT 52 2 IKKVGQKLCVDPAVIAGII LYG_STRCA 52 2 IKRVGEALCIEPAVIAGII LYG_CHICK 78 2 LYSOZYMEG4 Length of motif = 21 Motif number = 4 Lysozyme G motif IV - 2 PCODE ST INT SRESHAGKVLKNGWGDRGNGF LYG_ANSAN 71 0 SRESHAGKVLKNGWGDRGNGF LYG_CYGAT 71 0 SRESHAGKALRNGWGDNGNGF LYG_STRCA 71 0 SRESHAGKILKNGWGDRGNGF LYG_CHICK 97 0 LYSOZYMEG5 Length of motif = 19 Motif number = 5 Lysozyme G motif V - 2 PCODE ST INT GLMQVDKRSHKPQGTWNGE LYG_ANSAN 92 0 GLMQVDKRSHKPQGTWNGE LYG_CYGAT 92 0 GLMQVDRRSHKPVGEWNGE LYG_STRCA 92 0 GLMQVDKRYHKIEGTWNGE LYG_CHICK 118 0 LYSOZYMEG6 Length of motif = 17 Motif number = 6 Lysozyme G motif VI - 2 PCODE ST INT KTIQKKFPSWTKDQQLK LYG_ANSAN 125 14 KRIQKKFPSWTKDQQLK LYG_CYGAT 125 14 KAIQKKFPRWTKEQQLK LYG_STRCA 125 14 KKIQRKFPRWTRDQQLK LYG_CHICK 151 14 LYSOZYMEG7 Length of motif = 22 Motif number = 7 Lysozyme G motif VII - 2 PCODE ST INT GGISAYNAGAGNVRSYARMDIG LYG_ANSAN 142 0 GGISAYNAGAGNVRSYARMDIG LYG_CYGAT 142 0 GGISAYNAGPGNVRSYERMDIG LYG_STRCA 142 0 GGISAYNAGVGNVRSYERMDIG LYG_CHICK 168 0 LYSOZYMEG8 Length of motif = 21 Motif number = 8 Lysozyme G motif VIII - 2 PCODE ST INT TTHDDYANDVVARAQYYKQHG LYG_ANSAN 164 0 TTHDDYANDVVARAQYYKQHG LYG_CYGAT 164 0 TTHDDYANDVVARAQYYKQHG LYG_STRCA 164 0 TLHDDYSNDVVARAQYFKQHG LYG_CHICK 190 0

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