WORKLIST ENTRIES (1):

GLHYDRLASE8 View alignment      Glycosyl hydrolase family 8 signature
 Type of fingerprint: COMPOUND with 6  elements
Links:
   PRINTS; PR00131 GLHYDRLASE1; PR00132 GLHYDRLASE2; PR00133 GLHYDRLASE3
   PRINTS; PR00732 GLHYDRLASE4; PR00733 GLHYDRLASE6; PR00734 GLHYDRLASE7
   PRINTS; PR00134 GLHYDRLASE10; PR00911 GLHYDRLASE11; PR00736 GLHYDRLASE15
   PRINTS; PR00737 GLHYDRLASE16; PR00738 GLHYDRLASE20; PR00739 GLHYDRLASE26
   PRINTS; PR00740 GLHYDRLASE27; PR00741 GLHYDRLASE29; PR00843 GLHYDRLASE30
   PRINTS; PR00742 GLHYDRLASE35; PR00743 GLHYDRLASE36; PR00744 GLHYDRLASE37
   PRINTS; PR00745 GLHYDRLASE39; PR00746 GLHYDRLASE41; PR00747 GLHYDRLASE47
   PRINTS; PR00844 GLHYDRLASE48; PR00845 GLHYDRLASE52; PR00846 GLHYDRLASE56
   PRINTS; PR00849 GLHYDRLASE58; PR00850 GLHYDRLASE59; PR00748 MELIBIASE
   PRINTS; PR00137 LYSOZYME; PR00684 T4LYSOZYME; PR00749 LYSOZYMEG
   PRINTS; PR00110 ALPHAAMYLASE; PR00750 BETAAMYLASE
   INTERPRO; IPR002037
   PROSITE; PS00812 GLYCOSYL_HYDROL_F8
   PFAM; PF01270 Glycosyl_hydr20

 Creation date 03-JUN-1997; UPDATE 07-JUN-1999

   1. HENRISSAT, B. AND BAIROCH, A.
   New families in the classification of glycosyl hydrolases based on amino
   acid sequence similarities.
   BIOCHEM.J. 293 781-788 (1993).

   2. HENRISSAT, B.
   A classification of glycosyl hydrolases based on amino acid sequence
   similarities.
   BIOCHEM.J. 280 309-316 (1991).

   3. DAVIES, G. AND HENRISSAT, B.
   Structures and mechanisms of glycosyl hydrolases.
   STRUCTURE 3 853-859 (1995).

   4. HENRISSAT, B. AND BAIROCH, A.
   Updating the sequence-based classification of glycosyl hydrolases.
   BIOCHEM.J. 316 695-696 (1996).

   5. ALZARI, P.M., SOUCHON, H. AND DOMINGUEZ, R.
   The crystal structure of endoglucanase celA, a family8 glycosyl hydrolase
   from Clostridium thermocellum.
   STRUCTURE 4(3) 265-275 (1996).

   O-Glycosyl hydrolases (EC 3.2.1.-) are a widespread group of enzymes that
   hydrolyse the glycosidic bond between two or more carbohydrates, or between
   a carbohydrate and a non-carbohydrate moiety. A classification system for
   glycosyl hydrolases, based on sequence similarity, has led to the definition
   of up to 60 different families [1-4] (http://expasy.hcuge.ch/cgi-bin/lists?
   glycosid.txt). Family 8 includes mostly endoglucanases, which are essential
   enzymes for microbial degradation of cellulose and xylans. 
  
   GLHYDRLASE8 is a 6-element fingerprint that provides a signature for
   family 8 glycosyl hydrolases. The fingerprint was derived from an initial
   alignment of 8 sequences: the motifs were drawn from conserved regions
   spanning virtually the full alignment length - motif 2 includes the region
   encoded by PROSITE pattern GLYCOSYL_HYDROL_F8 (PS00812), the first 
   aspartate of which is thought to act as the nucleophile in the catalytic
   mechanism [5]. A single iteration on OWL29.3 was required to reach
   convergence, no further sequences being identified beyond the starting set.
   Several partial matches were found, all of which are family members that
   fail to make significant matches with one or more motifs.
  
   An update on SPTR37_9f identified a true set of 9 sequences, and 2
   partial matches.

  SUMMARY INFORMATION
      9 codes involving  6 elements
      0 codes involving  5 elements
      1 codes involving  4 elements
      1 codes involving  3 elements
      0 codes involving  2 elements

   COMPOSITE FINGERPRINT INDEX
  
    6|   9    9    9    9    9    9  
    5|   0    0    0    0    0    0  
    4|   1    1    1    0    1    0  
    3|   1    1    0    0    1    0  
    2|   0    0    0    0    0    0  
   --+-------------------------------
     |   1    2    3    4    5    6  

True positives..
 GUN2_CLOJO     GUNC_CLOCE     GUNA_CLOTM     GUNA_ACEXY     
 O82857         GUNY_ERWCH     GUN_BACSP      GUB_BACCI      
 YHJM_ECOLI     
Subfamily:  Codes involving 4 elements
 Subfamily True positives..
 GUN_CELUD      
Subfamily:  Codes involving 3 elements
 Subfamily True positives..
 Q44416         


  PROTEIN TITLES
   GUN2_CLOJO       ENDOGLUCANASE 2 PRECURSOR (EC 3.2.1.4) (ENDO-1,4-BETA-GLUCAN
   GUNC_CLOCE       ENDOGLUCANASE C PRECURSOR (EC 3.2.1.4) (ENDO-1,4-BETA-GLUCAN
   GUNA_CLOTM       ENDOGLUCANASE A PRECURSOR (EC 3.2.1.4) (EGA) (ENDO-1,4-BETA-
   GUNA_ACEXY       PROBABLE ENDOGLUCANASE PRECURSOR (EC 3.2.1.4) (ENDO-1,4-BETA
   O82857           ENDOGLUCANASE - ACETOBACTER XYLINUM (ACETOBACTER PASTEURIANU
   GUNY_ERWCH       MINOR ENDOGLUCANASE Y PRECURSOR (EC 3.2.1.4) (ENDO-1,4-BETA-
   GUN_BACSP        ENDOGLUCANASE PRECURSOR (EC 3.2.1.4) (ENDO-1,4-BETA-GLUCANAS
   GUB_BACCI        BETA-GLUCANASE PRECURSOR (EC 3.2.1.73) (ENDO-BETA-1,3-1,4 GL
   YHJM_ECOLI       HYPOTHETICAL 41.7 KD PROTEIN IN DCTA-DPPF INTERGENIC REGION 
 
   GUN_CELUD        ENDOGLUCANASE PRECURSOR (EC 3.2.1.4) (ENDO-1,4-BETA-GLUCANAS
 
   Q44416           ENDOGLUCANASE - AGROBACTERIUM TUMEFACIENS.

SCAN HISTORY OWL29_3 1 50 NSINGLE SPTR37_9f 2 22 NSINGLE INITIAL MOTIF SETS GLHYDRLASE81 Length of motif = 14 Motif number = 1 Glycosyl hydrolase family 8 motif I - 1 PCODE ST INT TVSEGLGYGMLLAV GUN2_CLOJO 96 96 TVSEGMGYGLLLAV GUNA_CLOTM 92 92 TVSEGLGYGLLLSV GUNC_CLOCE 96 96 SHSEGQGYGMLFAA GUNA_ACEXY 54 54 TVSEAHGYGMLATV GUB_BACCI 92 92 GTSEGQGYGMIITV GUN_BACSP 127 127 TTSEGQSYGMFSAL YHJM_ECOLI 52 52 SHTEGQGFAMLMAV GUNY_ERWCH 50 50 GLHYDRLASE82 Length of motif = 19 Motif number = 2 Glycosyl hydrolase family 8 motif II - 1 PCODE ST INT ATDADEDIAVSLVFAHKKW GUN2_CLOJO 153 43 ATDADEDIALALIFADKLW GUNA_CLOTM 150 44 ATDADEDIAVSLVFAHKKW GUNC_CLOCE 153 43 ATDGDLLIALALGRAGKRF GUNA_ACEXY 112 44 ATDGDMDIAYSLLLADKQW GUB_BACCI 154 48 ATDGDLDIAYSLLLAHKQW GUN_BACSP 189 48 ASDGDVWMAWSLLEAGRLW YHJM_ECOLI 114 48 ASDGDVLIAWALLKAGNKW GUNY_ERWCH 108 44 GLHYDRLASE83 Length of motif = 18 Motif number = 3 Glycosyl hydrolase family 8 motif III - 1 PCODE ST INT NPSYFAPAWYRIFADFTG GUN2_CLOJO 215 43 NPSYFAPAWYKVYAQYTG GUNA_CLOTM 212 43 NPSYFAPAWYRIFADFTG GUNC_CLOCE 215 43 NLSYYVMPSLLQAFDLTA GUNA_ACEXY 176 45 RPSDFMLNHLKAFQAATG GUB_BACCI 219 46 RPSDWMMSHLRAFYEFTG GUN_BACSP 252 44 NPSYLPPTLAQYFTRFGA YHJM_ECOLI 179 46 NPSYFLFPAWRDFANRSH GUNY_ERWCH 172 45 GLHYDRLASE84 Length of motif = 12 Motif number = 4 Glycosyl hydrolase family 8 motif IV - 1 PCODE ST INT TGLVPDWCTANG GUN2_CLOJO 255 22 TGLVPDWCTASG GUNA_CLOTM 254 24 TGLVPDWCTANG GUNC_CLOCE 255 22 WRLPPDWLAVNR GUNA_ACEXY 216 22 TGLLPDFVVLSG GUB_BACCI 262 25 TGLISDFVVKNP GUN_BACSP 295 25 KGFSPDWVRYEK YHJM_ECOLI 215 18 VGLPTDWAALNA GUNY_ERWCH 212 22 GLHYDRLASE85 Length of motif = 10 Motif number = 5 Glycosyl hydrolase family 8 motif V - 1 PCODE ST INT FYYDAIRYQW GUN2_CLOJO 276 9 YKYDATRYGW GUNA_CLOTM 275 9 FYYDAIRYQW GUNC_CLOCE 276 9 FSYDAIRVPL GUNA_ACEXY 242 14 YDYNSCRTPW GUB_BACCI 292 18 YYYNASRVPL GUN_BACSP 324 17 SSYDAIRVYM YHJM_ECOLI 240 13 FSYDAIRIPL GUNY_ERWCH 237 13 GLHYDRLASE86 Length of motif = 15 Motif number = 6 Glycosyl hydrolase family 8 motif VI - 1 PCODE ST INT YFGNTLRMMILLYTT GUN2_CLOJO 372 86 YYGNSLRLLTLLYIT GUNA_CLOTM 371 86 YFGNTLRMMVLLYTT GUNC_CLOCE 372 86 YYSAALTLLVYIARA GUNA_ACEXY 323 71 YYEDSIKLFSMIVMS GUB_BACCI 389 87 YFSDSYNLLTMLFLT GUN_BACSP 420 86 YYNYVLTLFGQGWDQ YHJM_ECOLI 331 81 YYSSSLRLLVMLARG GUNY_ERWCH 317 70 FINAL MOTIF SETS GLHYDRLASE81 Length of motif = 14 Motif number = 1 Glycosyl hydrolase family 8 motif I - 2 PCODE ST INT TVSEGLGYGMLLAV GUN2_CLOJO 96 96 TVSEGLGYGLLLSV GUNC_CLOCE 96 96 TVSEGMGYGLLLAV GUNA_CLOTM 92 92 SHSEGQGYGMLFAA GUNA_ACEXY 54 54 SHSEGQGYGMLFSA O82857 53 53 SHTEGQGFAMLMAV GUNY_ERWCH 50 50 GTSEGQGYGMIITV GUN_BACSP 127 127 TVSEAHGYGMLATV GUB_BACCI 92 92 TTSEGQSYGMFSAL YHJM_ECOLI 52 52 GLHYDRLASE82 Length of motif = 19 Motif number = 2 Glycosyl hydrolase family 8 motif II - 2 PCODE ST INT ATDADEDIAVSLVFAHKKW GUN2_CLOJO 153 43 ATDADEDIAVSLVFAHKKW GUNC_CLOCE 153 43 ATDADEDIALALIFADKLW GUNA_CLOTM 150 44 ATDGDLLIALALGRAGKRF GUNA_ACEXY 112 44 ATDGDLLIALALAWAGKRW O82857 111 44 ASDGDVLIAWALLKAGNKW GUNY_ERWCH 108 44 ATDGDLDIAYSLLLAHKQW GUN_BACSP 189 48 ATDGDMDIAYSLLLADKQW GUB_BACCI 154 48 ASDGDVWMAWSLLEAGRLW YHJM_ECOLI 114 48 GLHYDRLASE83 Length of motif = 18 Motif number = 3 Glycosyl hydrolase family 8 motif III - 2 PCODE ST INT NPSYFAPAWYRIFADFTG GUN2_CLOJO 215 43 NPSYFAPAWYRIFADFTG GUNC_CLOCE 215 43 NPSYFAPAWYKVYAQYTG GUNA_CLOTM 212 43 NLSYYVMPSLLQAFDLTA GUNA_ACEXY 176 45 NLSYYVMPSLMQAFALTG O82857 175 45 NPSYFLFPAWRDFANRSH GUNY_ERWCH 172 45 RPSDWMMSHLRAFYEFTG GUN_BACSP 252 44 RPSDFMLNHLKAFQAATG GUB_BACCI 219 46 NPSYLPPTLAQYFTRFGA YHJM_ECOLI 179 46 GLHYDRLASE84 Length of motif = 12 Motif number = 4 Glycosyl hydrolase family 8 motif IV - 2 PCODE ST INT TGLVPDWCTANG GUN2_CLOJO 255 22 TGLVPDWCTANG GUNC_CLOCE 255 22 TGLVPDWCTASG GUNA_CLOTM 254 24 WRLPPDWLAVNR GUNA_ACEXY 216 22 WKLPPDWLSINL O82857 215 22 VGLPTDWAALNA GUNY_ERWCH 212 22 TGLISDFVVKNP GUN_BACSP 295 25 TGLLPDFVVLSG GUB_BACCI 262 25 KGFSPDWVRYEK YHJM_ECOLI 215 18 GLHYDRLASE85 Length of motif = 10 Motif number = 5 Glycosyl hydrolase family 8 motif V - 2 PCODE ST INT FYYDAIRYQW GUN2_CLOJO 276 9 FYYDAIRYQW GUNC_CLOCE 276 9 YKYDATRYGW GUNA_CLOTM 275 9 FSYDAIRVPL GUNA_ACEXY 242 14 FSYDAIRVPL O82857 241 14 FSYDAIRIPL GUNY_ERWCH 237 13 YYYNASRVPL GUN_BACSP 324 17 YDYNSCRTPW GUB_BACCI 292 18 SSYDAIRVYM YHJM_ECOLI 240 13 GLHYDRLASE86 Length of motif = 15 Motif number = 6 Glycosyl hydrolase family 8 motif VI - 2 PCODE ST INT YFGNTLRMMILLYTT GUN2_CLOJO 372 86 YFGNTLRMMVLLYTT GUNC_CLOCE 372 86 YYGNSLRLLTLLYIT GUNA_CLOTM 371 86 YYSAALTLLVYIARA GUNA_ACEXY 323 71 YYSAALTMLAYIARN O82857 322 71 YYSSSLRLLVMLARG GUNY_ERWCH 317 70 YFSDSYNLLTMLFLT GUN_BACSP 420 86 YYEDSIKLFSMIVMS GUB_BACCI 389 87 YYNYVLTLFGQGWDQ YHJM_ECOLI 331 81

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