WORKLIST ENTRIES (1):

GLHYDRLASE6 View alignment      Glycosyl hydrolase family 6 signature
 Type of fingerprint: COMPOUND with 8  elements
Links:
   PRINTS; PR00131 GLHYDRLASE1; PR00132 GLHYDRLASE2; PR00133 GLHYDRLASE3
   PRINTS; PR00732 GLHYDRLASE4; PR00734 GLHYDRLASE7; PR00735 GLHYDRLASE8
   PRINTS; PR00134 GLHYDRLASE10; PR00911 GLHYDRLASE11; PR00736 GLHYDRLASE15
   PRINTS; PR00737 GLHYDRLASE16; PR00738 GLHYDRLASE20; PR00739 GLHYDRLASE26
   PRINTS; PR00740 GLHYDRLASE27; PR00741 GLHYDRLASE29; PR00843 GLHYDRLASE30
   PRINTS; PR00742 GLHYDRLASE35; PR00743 GLHYDRLASE36; PR00744 GLHYDRLASE37
   PRINTS; PR00745 GLHYDRLASE39; PR00746 GLHYDRLASE41; PR00747 GLHYDRLASE47
   PRINTS; PR00844 GLHYDRLASE48; PR00845 GLHYDRLASE52; PR00846 GLHYDRLASE56
   PRINTS; PR00849 GLHYDRLASE58; PR00850 GLHYDRLASE59; PR00748 MELIBIASE
   PRINTS; PR00137 LYSOZYME; PR00684 T4LYSOZYME; PR00749 LYSOZYMEG
   PRINTS; PR00110 ALPHAAMYLASE; PR00750 BETAAMYLASE
   INTERPRO; IPR001524
   PROSITE; PS00655 GLYCOSYL_HYDROL_F6_1; PS00656 GLYCOSYL_HYDROL_F6_2
   PFAM; PF01341 Glycosyl_hydr21
   PDB; 3CBH 3Dinfo
   SCOP; 3CBH

 Creation date 04-JUN-1997; UPDATE 21-JUN-1999

   1. HENRISSAT, B. AND BAIROCH, A.
   New families in the classification of glycosyl hydrolases based on amino
   acid sequence similarities.
   BIOCHEM.J. 293 781-788 (1993).

   2. HENRISSAT, B.
   A classification of glycosyl hydrolases based on amino acid sequence
   similarities.
   BIOCHEM.J. 280 309-316 (1991).

   3. DAVIES, G. AND HENRISSAT, B.
   Structures and mechanisms of glycosyl hydrolases.
   STRUCTURE 3 853-859 (1995).

   4. HENRISSAT, B. AND BAIROCH, A.
   Updating the sequence-based classification of glycosyl hydrolases.
   BIOCHEM.J. 316 695-696 (1996).

   5. ROUVINEN, J., BERGFORS, T., TEERI, T., KNOWLES, J.K.C. AND JONES, T.A.
   3-Dimensional structure of cellobiohydrolase-II from Trichoderma reesei.
   SCIENCE 249 380-386 (1990). 

   O-Glycosyl hydrolases (EC 3.2.1.-) are a widespread group of enzymes that
   hydrolyse the glycosidic bond between two or more carbohydrates, or between
   a carbohydrate and a non-carbohydrate moiety. A classification system for
   glycosyl hydrolases, based on sequence similarity, has led to the definition
   of up to 60 different families [1-4] (http://expasy.hcuge.ch/cgi-bin/lists?
   glycosid.txt). Family 6 includes endoglucanases and cellobiohydrolases,
   which are essential enzymes for microbial degradation of cellulose and
   xylans. 
  
   The 3D structure of the enzymatic core of cellobiohydrolase II (CBHII) from
   the fungus Trichoderma reesei reveals an alpha-beta protein with a fold
   similar to the ubiquitous barrel topology first seen in triose phosphate
   isomerase [5]. The active site of CBHII is located at the C-terminal end of
   a parallel beta barrel, in an enclosed tunnel through which the cellulose
   threads. Two aspartic acid residues, located in the center of the tunnel
   are the probable catalytic residues [5].
   
   GLHYDRLASE6 is an 8-element fingerprint that provides a signature for 
   family 6 glycosyl hydrolases. The fingerprint was derived from an initial
   alignment of 7 sequences: the motifs were drawn from conserved regions 
   within the catalytic domain - motif 2 includes part of the region encoded
   by PROSITE pattern GLYCOSYL_HYDROL_F6_1 (PS00655), which contains a
   conserved aspartic acid residue that may be involved in the catalytic
   mechanism [5]. Two iterations on OWL29.3 were required to reach convergence,
   at which point a true set comprising 16 sequences was identified. Several
   partial matches were also found, all of which are family members that fail
   to make significant matches with one or more motifs.
  
   An update on SPTR37_9f identified a true set of 18 sequences, and 1
   partial match.

  SUMMARY INFORMATION
     18 codes involving  8 elements
      0 codes involving  7 elements
      0 codes involving  6 elements
      0 codes involving  5 elements
      0 codes involving  4 elements
      0 codes involving  3 elements
      1 codes involving  2 elements

   COMPOSITE FINGERPRINT INDEX
  
    8|  18   18   18   18   18   18   18   18  
    7|   0    0    0    0    0    0    0    0  
    6|   0    0    0    0    0    0    0    0  
    5|   0    0    0    0    0    0    0    0  
    4|   0    0    0    0    0    0    0    0  
    3|   0    0    0    0    0    0    0    0  
    2|   1    0    0    0    0    1    0    0  
   --+-----------------------------------------
     |   1    2    3    4    5    6    7    8  

True positives..
 GUN2_THEFU     P78721         GUX3_AGABI     GUNA_MICBI     
 P78720         Q12646         GUNA_CELFI     Q02321         
 GUNB_FUSOX     GUN1_STRHA     O53607         O86730         
 GUN1_STRSQ     GUX2_TRIRE     Q50901         GUXA_CELFI     
 Q60029         Q53488         


  PROTEIN TITLES
   GUN2_THEFU       ENDOGLUCANASE E-2 PRECURSOR (EC 3.2.1.4) (ENDO-1,4-BETA-GLUC
   P78721           CELLULASE C (EC 3.2.1.4) (ENDOGLUCANASE) (ENDO-1,4-BETA-GLUC
   GUX3_AGABI       EXOGLUCANASE 3 PRECURSOR (EC 3.2.1.91) (EXOCELLOBIOHYDROLASE
   GUNA_MICBI       ENDOGLUCANASE A PRECURSOR (EC 3.2.1.4) (ENDO-1,4-BETA-GLUCAN
   P78720           CELLULASE A (EC 3.2.1.4) (ENDOGLUCANASE) (ENDO-1,4-BETA-GLUC
   Q12646           CELLOBIOHYDROLASE PRECURSOR (EC 3.2.1.91) (CELLULOSE 1,4-BET
   GUNA_CELFI       ENDOGLUCANASE A PRECURSOR (EC 3.2.1.4) (ENDO-1,4-BETA-GLUCAN
   Q02321           EXOCELLOBIOHYDROLASE - PHANEROCHAETE CHRYSOSPORIUM.
   GUNB_FUSOX       PUTATIVE ENDOGLUCANASE TYPE B PRECURSOR (EC 3.2.1.4) (ENDO-1
   GUN1_STRHA       ENDOGLUCANASE 1 PRECURSOR (EC 3.2.1.4) (ENDO-1,4-BETA-GLUCAN
   O53607           PUTATIVE CELLULASE - MYCOBACTERIUM TUBERCULOSIS.
   O86730           PUTATIVE SECRETED CELLULASE - STREPTOMYCES COELICOLOR.
   GUN1_STRSQ       ENDOGLUCANASE 1 PRECURSOR (EC 3.2.1.4) (ENDO-1,4-BETA-GLUCAN
   GUX2_TRIRE       EXOGLUCANASE II PRECURSOR (EC 3.2.1.91) (EXOCELLOBIOHYDROLAS
   Q50901           BETA-1,4-GLYCANASE - MYXOCOCCUS XANTHUS.
   GUXA_CELFI       EXOGLUCANASE A PRECURSOR (EC 3.2.1.91) (EXOCELLOBIOHYDROLASE
   Q60029           BETA-1,4-EXOCELLULASE PRECURSOR (EC 3.2.1.91) (CELLULOSE 1,4
   Q53488           ENDO-BETA-1,4-GLUCANASE - MICROMONOSPORA CELLULOLYTICUM.

SCAN HISTORY OWL29_3 3 100 NSINGLE SPTR37_9f 3 50 NSINGLE INITIAL MOTIF SETS GLHYDRLASE61 Length of motif = 20 Motif number = 1 Glycosyl hydrolase family 6 motif I - 1 PCODE ST INT NPSLATKAASVAKIPTFVWF GUX3_AGABI 116 116 DGRAAAINASIANTPMARWF GUN1_STRHA 55 55 SGAKATAAAKVADVPSFQWM GUNB_FUSOX 132 132 DPRTPVIRDRIAAVPTGRWF GUNA_MICBI 57 57 DPRTPVIRDRIASVPQGTWF GUN2_THEFU 54 54 DHRAPLIAERIGSQPQAVWF GUN1_STRSQ 97 97 SGTDKALLEKIALTPQAYWV GUNA_CELFI 190 190 GLHYDRLASE62 Length of motif = 14 Motif number = 2 Glycosyl hydrolase family 6 motif II - 1 PCODE ST INT QLVQIVVYDLPDRD GUX3_AGABI 156 20 KLPILVAYNIYNRD GUN1_STRHA 97 22 YAGQFVVYDLPNRD GUNB_FUSOX 177 25 KIPIMVVYAMPNRD GUNA_MICBI 100 23 KIPILVVYNAPGRD GUN2_THEFU 97 23 QLPVVVPYMIPFRD GUN1_STRSQ 141 24 KTPMLVVYAIPGRD GUNA_CELFI 234 24 GLHYDRLASE63 Length of motif = 16 Motif number = 3 Glycosyl hydrolase family 6 motif III - 1 PCODE ST INT YKNYVDQIAAQIKQFP GUX3_AGABI 189 19 YADWIARFAGGIAARP GUN1_STRHA 126 15 YKAYIAKIKGILQNYS GUNB_FUSOX 210 19 YRAWIDEIAAGLRNRP GUNA_MICBI 128 14 YRSWIDEFAAGLKNRP GUN2_THEFU 125 14 YAEWSGLFAAGLGSEP GUN1_STRSQ 169 14 YARWVDTVAQGIKGNP GUNA_CELFI 261 13 GLHYDRLASE64 Length of motif = 14 Motif number = 4 Glycosyl hydrolase family 6 motif IV - 1 PCODE ST INT GVTMYIDAGHAGWL GUX3_AGABI 251 46 NTWVYMDAGNPRWA GUN1_STRHA 182 40 NVSMYLDAGHGGWL GUNB_FUSOX 272 46 QAKVYFDAGHDAWV GUNA_MICBI 185 41 QARIYFDAGHSAWH GUN2_THEFU 181 40 EARVYYDVGHSAWH GUN1_STRSQ 225 40 GARVYIDAGHAKWL GUNA_CELFI 312 35 GLHYDRLASE65 Length of motif = 11 Motif number = 5 Glycosyl hydrolase family 6 motif V - 1 PCODE ST INT LRGIATNVANF GUX3_AGABI 290 25 AHGFSLNVSNY GUN1_STRHA 212 16 VRGLVTNVSNY GUNB_FUSOX 311 25 ADGIALNVSNY GUNA_MICBI 216 17 AHGIATNTSNY GUN2_THEFU 212 17 GAGIATNISNY GUN1_STRSQ 256 17 AVGFALNTSNY GUNA_CELFI 342 16 GLHYDRLASE66 Length of motif = 14 Motif number = 6 Glycosyl hydrolase family 6 motif VI - 1 PCODE ST INT AHFIVDQGRSGVQN GUX3_AGABI 338 37 KPFVVDTSRNGNGS GUN1_STRHA 247 24 VKFIVDQGRSGKQP GUNB_FUSOX 360 38 LRAVIDTSRNGNGP GUNA_MICBI 248 21 LRAVIDTSRNGNGP GUN2_THEFU 244 21 LGAVVDTSRNGNGP GUN1_STRSQ 287 20 KKFVIDTSRNGNGS GUNA_CELFI 372 19 GLHYDRLASE67 Length of motif = 21 Motif number = 7 Glycosyl hydrolase family 6 motif VII - 1 PCODE ST INT WGDWCNVKGAGFGQRPTTNTG GUX3_AGABI 356 4 NGEWCNPSGRRIGTPTRTGGG GUN1_STRHA 261 0 QGDWCNAKGTGFGLRPSTNTG GUNB_FUSOX 379 5 GSEWCDPPGRATGTWSTTDTG GUNA_MICBI 263 1 GNEWCDPSGRAIGTPSTTNTG GUN2_THEFU 259 1 GSEWCDPPGRLVGNNPTVNPG GUN1_STRSQ 302 1 NGEWCNPRGRALGERPVAVND GUNA_CELFI 386 0 GLHYDRLASE68 Length of motif = 15 Motif number = 8 Glycosyl hydrolase family 6 motif VIII - 1 PCODE ST INT IDAIVWVKPGGECDG GUX3_AGABI 380 3 AEMLLWIKTPGESDG GUN1_STRHA 282 0 ADAFVWVKPGGESDG GUNB_FUSOX 403 3 IDAFLWIKPPGEADG GUNA_MICBI 287 3 IDAFLWIKLPGEADG GUN2_THEFU 283 3 VDAFLWIKLPGELDG GUN1_STRSQ 326 3 LDALLWVKLPGESDG GUNA_CELFI 410 3 FINAL MOTIF SETS GLHYDRLASE61 Length of motif = 20 Motif number = 1 Glycosyl hydrolase family 6 motif I - 3 PCODE ST INT DPRTPVIRDRIASVPQGTWF GUN2_THEFU 54 54 SGSLQEKAKKVKYVPTAAWL P78721 155 155 NPSLATKAASVAKIPTFVWF GUX3_AGABI 116 116 DPRTPVIRDRIAAVPTGRWF GUNA_MICBI 57 57 NGDLKAKAEKVKYVPTAVWL P78720 156 156 SYDLQQKAQKVKNVPTAVWL Q12646 131 131 SGTDKALLEKIALTPQAYWV GUNA_CELFI 190 190 DPTLSSKAASVANIPTFTWL Q02321 132 132 SGAKATAAAKVADVPSFQWM GUNB_FUSOX 132 132 DGRAAAINASIANTPMARWF GUN1_STRHA 55 55 ANPPNAELTSVANTPQSYWL O53607 111 111 NAAAEPGGDRIADEPTGVWL O86730 183 183 DHRAPLIAERIGSQPQAVWF GUN1_STRSQ 97 97 TGAMATAAAAVAKVPSFMWL GUX2_TRIRE 141 141 DSRAARIQSSVANNLAARWF Q50901 225 225 DPALAAKMRTVAGQPTAVWM GUXA_CELFI 74 74 KAAAEPGGSAVANESTAVWL Q60029 197 197 DFRAAVIREKIASQPQARWY Q53488 58 58 GLHYDRLASE62 Length of motif = 14 Motif number = 2 Glycosyl hydrolase family 6 motif II - 3 PCODE ST INT KIPILVVYNAPGRD GUN2_THEFU 97 23 KTVVFVLYMIPTRD P78721 193 18 QLVQIVVYDLPDRD GUX3_AGABI 156 20 KIPIMVVYAMPNRD GUNA_MICBI 100 23 KTVVFVLYMIPTRD P78720 194 18 KTVVFIMYMIPTRD Q12646 169 18 KTPMLVVYAIPGRD GUNA_CELFI 234 24 QLVQIVIYDLPDRD Q02321 178 26 YAGQFVVYDLPNRD GUNB_FUSOX 177 25 KLPILVAYNIYNRD GUN1_STRHA 97 22 AMPVLTLYGIPHRD O53607 155 24 MVVQLVIYNLPGRD O86730 233 30 QLPVVVPYMIPFRD GUN1_STRSQ 141 24 YAGQFVVYDLPDRD GUX2_TRIRE 186 25 KLPVLVAYNIPGRD Q50901 267 22 LVFNLVIYDLPGRD GUXA_CELFI 126 32 LTIQVVIYNLPGRD Q60029 251 34 QIPVLSVYEITNRD Q53488 101 23 GLHYDRLASE63 Length of motif = 16 Motif number = 3 Glycosyl hydrolase family 6 motif III - 3 PCODE ST INT YRSWIDEFAAGLKNRP GUN2_THEFU 125 14 YQGYVNSIYNTINQYP P78721 222 15 YKNYVDQIAAQIKQFP GUX3_AGABI 189 19 YRAWIDEIAAGLRNRP GUNA_MICBI 128 14 YKGYINNIYNTSNQYK P78720 223 15 YKGYVDNIARTIRSYP Q12646 198 15 YARWVDTVAQGIKGNP GUNA_CELFI 261 13 YENYIDQIVAQIQQFP Q02321 211 19 YKAYIAKIKGILQNYS GUNB_FUSOX 210 19 YADWIARFAGGIAARP GUN1_STRHA 126 15 YRGWIDAVASGLGSSP O53607 183 14 KTEYIDPIAEILSDSK O86730 265 18 YAEWSGLFAAGLGSEP GUN1_STRSQ 169 14 YKNYIDTIRQIVVEYS GUX2_TRIRE 219 19 YRTWISSFAAAIGNRP Q50901 295 14 KSEYIDPIADLLDNPE GUXA_CELFI 160 20 YVNGVGYALRKLGEIP Q60029 339 74 YQTWVSNFARGLGNQT Q53488 129 14 GLHYDRLASE64 Length of motif = 14 Motif number = 4 Glycosyl hydrolase family 6 motif IV - 3 PCODE ST INT QARIYFDAGHSAWH GUN2_THEFU 181 40 NVRVYLDAAHGGWL P78721 284 46 GVTMYIDAGHAGWL GUX3_AGABI 251 46 QAKVYFDAGHDAWV GUNA_MICBI 185 41 HVKVYLDAAHGAWL P78720 285 46 NVSVYLDAAHGAWL Q12646 260 46 GARVYIDAGHAKWL GUNA_CELFI 312 35 GVYMYMDAGHAGWL Q02321 273 46 NVSMYLDAGHGGWL GUNB_FUSOX 272 46 NTWVYMDAGNPRWA GUN1_STRHA 182 40 AAAVYVDAGHSRWL O53607 238 39 NVYNYVDAGHHGWL O86730 337 56 EARVYYDVGHSAWH GUN1_STRSQ 225 40 NVAMYLDAGHAGWL GUX2_TRIRE 281 46 NTWAYLDAGNALWI Q50901 352 41 NVYNYIDIGHSGWL GUXA_CELFI 225 49 NVYNYIDAAHHGWI Q60029 355 0 NAKVYLDGGHSTWN Q53488 185 40 GLHYDRLASE65 Length of motif = 11 Motif number = 5 Glycosyl hydrolase family 6 motif V - 3 PCODE ST INT AHGIATNTSNY GUN2_THEFU 212 17 IRGISTNVSNY P78721 320 22 LRGIATNVANF GUX3_AGABI 290 25 ADGIALNVSNY GUNA_MICBI 216 17 LRGISTNVSNY P78720 321 22 IRGLSTNISNY Q12646 296 22 AVGFALNTSNY GUNA_CELFI 342 16 IKGLATNVANY Q02321 312 25 VRGLVTNVSNY GUNB_FUSOX 311 25 AHGFSLNVSNY GUN1_STRHA 212 16 ARGFSLNVSNF O53607 268 16 VHGFIVNTANY O86730 377 26 GAGIATNISNY GUN1_STRSQ 256 17 LRGLATNVANY GUX2_TRIRE 320 25 VRGFALNVSNF Q50901 382 16 IDGFVSDVANT GUXA_CELFI 265 26 VHGFISNTANY Q60029 395 26 ADGFFTNVSNF Q53488 215 16 GLHYDRLASE66 Length of motif = 14 Motif number = 6 Glycosyl hydrolase family 6 motif VI - 3 PCODE ST INT LRAVIDTSRNGNGP GUN2_THEFU 244 21 MKFIVDTSRNGRNP P78721 355 24 AHFIVDQGRSGVQN GUX3_AGABI 338 37 LRAVIDTSRNGNGP GUNA_MICBI 248 21 LKFIVDTSRNGANV P78720 356 24 MHFIVDTGRNGVTI Q12646 331 24 KKFVIDTSRNGNGS GUNA_CELFI 372 19 ATFIVDQGRSGVQN Q02321 360 37 VKFIVDQGRSGKQP GUNB_FUSOX 360 38 KPFVVDTSRNGNGS GUN1_STRHA 247 24 SHYVIDTSRNGAGP O53607 298 19 LGMLIDTSRNGWGG O86730 440 52 LGAVVDTSRNGNGP GUN1_STRSQ 287 20 AFFITDQGRSGKQP GUX2_TRIRE 369 38 KPFVVDTSRNGNGS Q50901 417 24 IGMLVDTSRNGWGG GUXA_CELFI 329 53 IGMLIDTSRNGWGG Q60029 458 52 KRQVIDTSRNGGAA Q53488 250 24 GLHYDRLASE67 Length of motif = 21 Motif number = 7 Glycosyl hydrolase family 6 motif VII - 3 PCODE ST INT GNEWCDPSGRAIGTPSTTNTG GUN2_THEFU 259 1 SATWCNLKGAGLGARPQANPD P78721 370 1 WGDWCNVKGAGFGQRPTTNTG GUX3_AGABI 356 4 GSEWCDPPGRATGTWSTTDTG GUNA_MICBI 263 1 SGTWCNFKGAGLGQRPKGNPN P78720 376 6 SGTWCNLVGTGLGERPRGNPN Q12646 346 1 NGEWCNPRGRALGERPVAVND GUNA_CELFI 386 0 WGDWCNIKGAGFGTRPTTNTG Q02321 378 4 QGDWCNAKGTGFGLRPSTNTG GUNB_FUSOX 379 5 NGEWCNPSGRRIGTPTRTGGG GUN1_STRHA 261 0 PLNWCNPSGRALGAPPTTATA O53607 316 4 LGNWCNQSGAGLGERPQASPA O86730 481 27 GSEWCDPPGRLVGNNPTVNPG GUN1_STRSQ 302 1 WGDWCNVIGTGFGIRPSANTG GUX2_TRIRE 388 5 NGEWCNPAGRKIGTTNQVGVG Q50901 431 0 RGAWCNPLGAGIGRFPEATPS GUXA_CELFI 370 27 PGNWCNQAGAGLGERPTVNPA Q60029 499 27 DWCADDNTDRRIGQYPTTNTG Q53488 265 1 GLHYDRLASE68 Length of motif = 15 Motif number = 8 Glycosyl hydrolase family 6 motif VIII - 3 PCODE ST INT IDAFLWIKLPGEADG GUN2_THEFU 283 3 LDAYVWIKTPGESDS P78721 396 5 IDAIVWVKPGGECDG GUX3_AGABI 380 3 IDAFLWIKPPGEADG GUNA_MICBI 287 3 LDAYMWIKTPGEADG P78720 403 6 LDAYMWLKTPGESDG Q12646 372 5 LDALLWVKLPGESDG GUNA_CELFI 410 3 IDSIVWVKPGGECDG Q02321 402 3 ADAFVWVKPGGESDG GUNB_FUSOX 403 3 AEMLLWIKTPGESDG GUN1_STRHA 282 0 ADAYLWIKRPGESDG O53607 340 3 IDAYVWMKPPGESDG O86730 504 2 VDAFLWIKLPGELDG GUN1_STRSQ 326 3 LDSFVWVKPGGECDG GUX2_TRIRE 412 3 AEMTVWIKVPGDSDG Q50901 453 1 LDAFVWIKPPGESDG GUXA_CELFI 397 6 VDAYVWVKPPGESDG Q60029 522 2 IDAYLWVKPPGEADG Q53488 289 3

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