WORKLIST ENTRIES (1):

GLHYDRLASE58 View alignment     Glycosyl hydrolase family 58 signature
 Type of fingerprint: COMPOUND with 8  elements
Links:
   PRINTS; PR00131 GLHYDRLASE1; PR00132 GLHYDRLASE2; PR00133 GLHYDRLASE3
   PRINTS; PR00732 GLHYDRLASE4; PR00733 GLHYDRLASE6; PR00734 GLHYDRLASE7
   PRINTS; PR00735 GLHYDRLASE8; PR00134 GLHYDRLASE10; PR00911 GLHYDRLASE11
   PRINTS; PR00736 GLHYDRLASE15; PR00737 GLHYDRLASE16; PR00738 GLHYDRLASE20
   PRINTS; PR00739 GLHYDRLASE26; PR00740 GLHYDRLASE27; PR00741 GLHYDRLASE29
   PRINTS; PR00843 GLHYDRLASE30; PR00742 GLHYDRLASE35; PR00743 GLHYDRLASE36
   PRINTS; PR00744 GLHYDRLASE37; PR00745 GLHYDRLASE39; PR00746 GLHYDRLASE41
   PRINTS; PR00747 GLHYDRLASE47; PR00844 GLHYDRLASE48; PR00845 GLHYDRLASE52
   PRINTS; PR00846 GLHYDRLASE56; PR00850 GLHYDRLASE59; PR00748 MELIBIASE
   PRINTS; PR00137 LYSOZYME; PR00684 T4LYSOZYME; PR00749 LYSOZYMEG
   PRINTS; PR00110 ALPHAAMYLASE; PR00750 BETAAMYLASE
   INTERPRO; IPR001724

 Creation date 05-MAR-1998; UPDATE 21-JUN-1999

   1. HENRISSAT, B.
   A classification of glycosyl hydrolases based on amino acid sequence
   similarities.
   BIOCHEM.J. 280 309-316 (1991).

   2. HENRISSAT, B. AND BAIROCH, A.
   New families in the classification of glycosyl hydrolases based on amino
   acid sequence similarities.
   BIOCHEM.J. 293 781-788 (1993).

   3. HENRISSAT, B. AND BAIROCH, A.
   Updating the sequence-based classification of glycosyl hydrolases.
   BIOCHEM.J. 316 695-696 (1996).

   4. EL HASSOUNI, M., HENRISSAT, B., CHIPPAUX, M. AND BARRAS, F.
   Nucleotide sequences of the Arb genes, which control beta-glucosidase
   utilisation in Erwinia chrysanthemi - Comparison with the Escherichia
   coli Bgl operon and evidence for a new beta-glycohydrolase family
   including enzymes from eubacteria, archaebacteria and humans.
   J.BACTERIOL. 174 765-777 (1992).
  
   5. LONG, G.S., BRYANT, J.M., TAYLOR, P.W., LUZIO, J.P.
   Complete nucleotide sequence of the gene encoding bacteriophage E 
   endosialidase: implications for K1E endosialidase structure and function. 
   BIOCHEM.J. 309 543-550 (1995).

   6. PETTER, J.G. AND VIMR, E.R.
   Complete nucleotide sequence of the bacteriophage K1F tail gene encoding 
   endo-n-acylneuraminidase (endo-N) and comparison to an endo-N homolog in
   bacteriophage PK1E. 
   J.BACTERIOL. 175 4354-4363 (1993). 

   O-Glycosyl hydrolases (EC 3.2.1.-) are a widespread group of enzymes that
   hydrolyse the glycosidic bond between two or more carbohydrates, or between
   a carbohydrate and a non-carbohydrate moiety. A classification system for
   glycosyl hydrolases, based on sequence similarity, has led to the definition
   of up to 60 different families [1-4] (http://expasy.hcuge.ch/cgi-bin/lists?
   glycosid.txt). Family 58 includes the endo-N-acetyl-neuraminidases (EC 
   3.2.1.129).
  
   Bacteriophage E specifically recognises and infects strains of E.coli
   that display the alpha-2,8-linked polysialic acid K1 capsule [5,6]. 
   Bacteriophage E endosialidase is thought to be responsible for initial
   absorption of the phage to the host bacterium [5]. The native enzyme is
   probably a trimer of identical 74kDa subunits. Within the K1E endosialidase
   sequence, a central region of 500 amino acids shows 84% identity to K1F 
   endosialidase [5]. Both enzymes contain two copies of a sialidase sequence
   motif common to many bacterial and viral sialidases. These motifs flank
   the region of greatest identity between the two endosialidases, suggesting
   that this domain is involved in binding and hydrolysis of the polysialic
   acid substrate [5].
  
   GLHYDRLASE58 is an 8-element fingerprint that provides a signature for
   family 58 glycosyl hydrolases. The fingerprint was derived from an initial
   alignment of 2 sequences: the motifs were drawn from conserved regions
   spanning the C-terminal half of the alignment. Two iterations on OWL30.0
   were required to reach convergence, at which point a true set comprising 
   3 sequences was identified.
  
   An update on SPTR37_9f identified a true set of 3 sequences.

  SUMMARY INFORMATION
      3 codes involving  8 elements
      0 codes involving  7 elements
      0 codes involving  6 elements
      0 codes involving  5 elements
      0 codes involving  4 elements
      0 codes involving  3 elements
      0 codes involving  2 elements

   COMPOSITE FINGERPRINT INDEX
  
    8|   3    3    3    3    3    3    3    3  
    7|   0    0    0    0    0    0    0    0  
    6|   0    0    0    0    0    0    0    0  
    5|   0    0    0    0    0    0    0    0  
    4|   0    0    0    0    0    0    0    0  
    3|   0    0    0    0    0    0    0    0  
    2|   0    0    0    0    0    0    0    0  
   --+-----------------------------------------
     |   1    2    3    4    5    6    7    8  

True positives..
 ENAN_BPK1E     ENAN_BPK1F     O80102         


  PROTEIN TITLES
   ENAN_BPK1E       ENDO-N-ACETYLNEURAMINIDASE (EC 3.2.1.129) (ENDO-N) (ENDOSIAL
   ENAN_BPK1F       ENDO-N-ACETYLNEURAMINIDASE (EC 3.2.1.129) (ENDO-N) (ENDOSIAL
   O80102           ENDO-N-ACETYLNEURAMINIDASE - BACTERIOPHAGE #D.

SCAN HISTORY OWL30_0 2 75 NSINGLE SPTR37_9f 2 20 NSINGLE INITIAL MOTIF SETS GLHYDRLASE581 Length of motif = 25 Motif number = 1 Glycosyl hydrolase family 58 motif I - 1 PCODE ST INT GELYKITDNPYYNAWPQDKAFVYEN ENAN_BPK1E 104 104 GELFKITDTPYYNAWPQDKAFVYEN ENAN_BPK1F 313 313 GLHYDRLASE582 Length of motif = 29 Motif number = 2 Glycosyl hydrolase family 58 motif II - 1 PCODE ST INT HPDYPTVNYHCMSMGVCRNRLFAMIETRT ENAN_BPK1E 169 40 HPDYPTVNYHCMSMGVCRNRLFAMIETRT ENAN_BPK1F 378 40 GLHYDRLASE583 Length of motif = 24 Motif number = 3 Glycosyl hydrolase family 58 motif III - 1 PCODE ST INT GKNWHMGTSFHKSPWRKTDLGLIP ENAN_BPK1E 288 90 GKNWHMGTSFHKSPWRKTDLGLIP ENAN_BPK1F 497 90 GLHYDRLASE584 Length of motif = 27 Motif number = 4 Glycosyl hydrolase family 58 motif IV - 1 PCODE ST INT RRQIPSEYEPDAAEPCIKYYDGVLYLI ENAN_BPK1E 358 46 RRQIPSEYEPDASEPCIKYYDGVLYLI ENAN_BPK1F 567 46 GLHYDRLASE585 Length of motif = 27 Motif number = 5 Glycosyl hydrolase family 58 motif V - 1 PCODE ST INT ERAENEWEAGAPDDRYKASYPRTFYAR ENAN_BPK1E 436 51 ERAENEWEAGAPDDRYKASYPRTFYAR ENAN_BPK1F 645 51 GLHYDRLASE586 Length of motif = 25 Motif number = 6 Glycosyl hydrolase family 58 motif VI - 1 PCODE ST INT VGVGSVVVKDSFIYYIFGGENHFNP ENAN_BPK1E 492 29 VGVGSVVVKDNYIYYMFGGEDHFNP ENAN_BPK1F 701 29 GLHYDRLASE587 Length of motif = 27 Motif number = 7 Glycosyl hydrolase family 58 motif VII - 1 PCODE ST INT LKASTSANIRSEMQMEGEYGFIGKSVP ENAN_BPK1E 598 81 IRASTSSNIRSEVLMEGEYGFIGKSIP ENAN_BPK1F 802 76 GLHYDRLASE588 Length of motif = 28 Motif number = 8 Glycosyl hydrolase family 58 motif VIII - 1 PCODE ST INT GQRLIICGGEGTSSSSGAQITLHGSNSS ENAN_BPK1E 630 5 GQRIIFCGGEGTSSTTGAQITLYGANNT ENAN_BPK1F 834 5 FINAL MOTIF SETS GLHYDRLASE581 Length of motif = 25 Motif number = 1 Glycosyl hydrolase family 58 motif I - 2 PCODE ST INT GELYKITDNPYYNAWPQDKAFVYEN ENAN_BPK1E 104 104 GELFKITDTPYYNAWPQDKAFVYEN ENAN_BPK1F 313 313 GSLTKVTDTPFYNAWTQDKTFVYDH O80102 235 235 GLHYDRLASE582 Length of motif = 29 Motif number = 2 Glycosyl hydrolase family 58 motif II - 2 PCODE ST INT HPDYPTVNYHCMSMGVCRNRLFAMIETRT ENAN_BPK1E 169 40 HPDYPTVNYHCMSMGVCRNRLFAMIETRT ENAN_BPK1F 378 40 SVSADRVNYHCMSMGTCGNRLYAVIETRY O80102 306 46 GLHYDRLASE583 Length of motif = 24 Motif number = 3 Glycosyl hydrolase family 58 motif III - 2 PCODE ST INT GKNWHMGTSFHKSPWRKTDLGLIP ENAN_BPK1E 288 90 GKNWHMGTSFHKSPWRKTDLGLIP ENAN_BPK1F 497 90 GVTWNFATRFWDSPWEITELPEVA O80102 425 90 GLHYDRLASE584 Length of motif = 27 Motif number = 4 Glycosyl hydrolase family 58 motif IV - 2 PCODE ST INT RRQIPSEYEPDAAEPCIKYYDGVLYLI ENAN_BPK1E 358 46 RRQIPSEYEPDASEPCIKYYDGVLYLI ENAN_BPK1F 567 46 RRTVAQAYADNASEPCVKSYGGVLYLT O80102 504 55 GLHYDRLASE585 Length of motif = 27 Motif number = 5 Glycosyl hydrolase family 58 motif V - 2 PCODE ST INT ERAENEWEAGAPDDRYKASYPRTFYAR ENAN_BPK1E 436 51 ERAENEWEAGAPDDRYKASYPRTFYAR ENAN_BPK1F 645 51 ERAFGEWEGGAPDSRYEGSYPRTFMCK O80102 582 51 GLHYDRLASE586 Length of motif = 25 Motif number = 6 Glycosyl hydrolase family 58 motif VI - 2 PCODE ST INT VGVGSVVVKDSFIYYIFGGENHFNP ENAN_BPK1E 492 29 VGVGSVVVKDNYIYYMFGGEDHFNP ENAN_BPK1F 701 29 VGVGSVCVKDGWLYYIFGGEDFFSP O80102 640 31 GLHYDRLASE587 Length of motif = 27 Motif number = 7 Glycosyl hydrolase family 58 motif VII - 2 PCODE ST INT LKASTSANIRSEMQMEGEYGFIGKSVP ENAN_BPK1E 598 81 IRASTSSNIRSEVLMEGEYGFIGKSIP ENAN_BPK1F 802 76 LEYDGTQQSAVRARFDGDYGLISKTVP O80102 744 79 GLHYDRLASE588 Length of motif = 28 Motif number = 8 Glycosyl hydrolase family 58 motif VIII - 2 PCODE ST INT GQRLIICGGEGTSSSSGAQITLHGSNSS ENAN_BPK1E 630 5 GQRIIFCGGEGTSSTTGAQITLYGANNT ENAN_BPK1F 834 5 RQRLIVSGGDGASSSDGALLQLYGSNHA O80102 776 5

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