WORKLIST ENTRIES (1):

GLHYDRLASE48 View alignment     Glycosyl hydrolase family 48 signature
 Type of fingerprint: COMPOUND with 9  elements
Links:
   PRINTS; PR00131 GLHYDRLASE1; PR00132 GLHYDRLASE2; PR00133 GLHYDRLASE3
   PRINTS; PR00732 GLHYDRLASE4; PR00733 GLHYDRLASE6; PR00734 GLHYDRLASE7
   PRINTS; PR00735 GLHYDRLASE8; PR00134 GLHYDRLASE10; PR00911 GLHYDRLASE11
   PRINTS; PR00736 GLHYDRLASE15; PR00737 GLHYDRLASE16; PR00738 GLHYDRLASE20
   PRINTS; PR00739 GLHYDRLASE26; PR00740 GLHYDRLASE27; PR00741 GLHYDRLASE29
   PRINTS; PR00843 GLHYDRLASE30; PR00742 GLHYDRLASE35; PR00743 GLHYDRLASE36
   PRINTS; PR00744 GLHYDRLASE37; PR00745 GLHYDRLASE39; PR00746 GLHYDRLASE41
   PRINTS; PR00747 GLHYDRLASE47; PR00845 GLHYDRLASE52; PR00846 GLHYDRLASE56
   PRINTS; PR00849 GLHYDRLASE58; PR00850 GLHYDRLASE59; PR00748 MELIBIASE
   PRINTS; PR00137 LYSOZYME; PR00684 T4LYSOZYME; PR00749 LYSOZYMEG
   PRINTS; PR00110 ALPHAAMYLASE; PR00750 BETAAMYLASE
   INTERPRO; IPR000556

 Creation date 20-FEB-1998; UPDATE 07-JUN-1999

   1. HENRISSAT, B.
   A classification of glycosyl hydrolases based on amino acid sequence 
   similarities.
   BIOCHEM.J. 280 309-316 (1991).

   2. HENRISSAT, B. AND BAIROCH, A.
   New families in the classification of glycosyl hydrolases based on amino
   acid sequence similarities.
   BIOCHEM.J. 293 781-788 (1993).

   3. HENRISSAT, B. AND BAIROCH, A.
   Updating the sequence-based classification of glycosyl hydrolases.
   BIOCHEM.J. 316 695-696 (1996).

   4. EL HASSOUNI, M., HENRISSAT, B., CHIPPAUX, M. AND BARRAS, F.
   Nucleotide sequences of the Arb genes, which control beta-glucosidase
   utilisation in Erwinia chrysanthemi - Comparison with the Escherichia
   coli Bgl operon and evidence for a new beta-glycohydrolase family
   including enzymes from eubacteria, archaebacteria and humans.
   J.BACTERIOL. 174 765-777 (1992).
  
   5. TE'O, V.S., SAUL, D.J. AND BERGQUIST, P.L.
   CelA, another gene coding for a multidomain cellulase from the extreme
   thermophile caldocellum saccharolyticum. 
   APPL.MICROBIOL.BIOTECHNOL. 43 291-296 (1995). 

   O-Glycosyl hydrolases (EC 3.2.1.-) are a widespread group of enzymes that
   hydrolyse the glycosidic bond between two or more carbohydrates, or between
   a carbohydrate and a non-carbohydrate moiety. A classification system for
   glycosyl hydrolases, based on sequence similarity, has led to the definition
   of up to 60 different families [1-4] (http://expasy.hcuge.ch/cgi-bin/lists?
   glycosid.txt). Family 48 encompasses endoglucanases (EC 3.2.1.4) and 
   cellobiohydrolases (EC 3.2.1.91).
  
   The largest cellulase gene sequenced to date is one of the cellulases (celA)
   from the genome of the thermophilic anaerobic bacterium Caldocellum 
   saccharolyticum [5]. The celA gene product is a polypeptide of 1751 amino
   acids; this has a multidomain structure comprising two catalytic domains 
   and two cellulose-binding domains, linked by Pro-Thr-rich regions (so-called
   PT linkers) [5]. The N-terminal domain encodes an endoglucanase activity on
   carboxymethylcellulose, consistent with its similarity to several endo-1, 
   4-beta-D-glucanase sequences. The C-terminal domain shows similarity to a 
   cellulase from Clostridium thermocellum (CelS), which acts synergistically
   with a second component to hydrolyse crystalline cellulose [5]. 
  
   GLHYDRLASE48 is a 9-element fingerprint that provides a signature for
   family 48 glycosyl hydrolases. The fingerprint was derived from an initial
   alignment of 5 sequences: the motifs were drawn from conserved regions 
   spanning the C-terminal portion of the alignment, which corresponds to the
   second catalytic domain, downstream from the Pro-Thr-rich region in celA 
   from C.saccharolyticum. Two iterations on OWL30.0 were required to reach
   convergence, at which point a true set comprising 6 sequences was identified.
  
   An update on SPTR37_9f identified a true set of 7 sequences, and 1
   partial match.

  SUMMARY INFORMATION
      7 codes involving  9 elements
      1 codes involving  8 elements
      0 codes involving  7 elements
      0 codes involving  6 elements
      0 codes involving  5 elements
      0 codes involving  4 elements
      0 codes involving  3 elements
      0 codes involving  2 elements

   COMPOSITE FINGERPRINT INDEX
  
    9|   7    7    7    7    7    7    7    7    7  
    8|   1    0    1    1    1    1    1    1    1  
    7|   0    0    0    0    0    0    0    0    0  
    6|   0    0    0    0    0    0    0    0    0  
    5|   0    0    0    0    0    0    0    0    0  
    4|   0    0    0    0    0    0    0    0    0  
    3|   0    0    0    0    0    0    0    0    0  
    2|   0    0    0    0    0    0    0    0    0  
   --+----------------------------------------------
     |   1    2    3    4    5    6    7    8    9  

True positives..
 O82831         GUNA_CALSA     GUNF_CLOCE     GUX2_CLOSR     
 GUNS_CLOTM     O86728         GUXB_CELFI     
Subfamily:  Codes involving 8 elements
 Subfamily True positives..
 O65986         


  PROTEIN TITLES
   O82831           EXOGLUCANASE - CLOSTRIDIUM JOSUI.
   GUNA_CALSA       ENDOGLUCANASE A PRECURSOR (EC 3.2.1.4) (ENDO-1,4-BETA-GLUCAN
   GUNF_CLOCE       ENDOGLUCANASE F PRECURSOR (EC 3.2.1.4) (ENDO-1,4-BETA-GLUCAN
   GUX2_CLOSR       EXOGLUCANASE II PRECURSOR (EC 3.2.1.91) (EXOCELLOBIOHYDROLAS
   GUNS_CLOTM       ENDOGLUCANASE SS PRECURSOR (EC 3.2.1.4) (EGSS) (ENDO-1,4-BET
   O86728           PUTATIVE SECRETED CELLULASE - STREPTOMYCES COELICOLOR.
   GUXB_CELFI       EXOGLUCANASE B PRECURSOR (EC 3.2.1.91) (EXOCELLOBIOHYDROLASE
 
   O65986           EXOGLUCANASE S (EC 3.2.1.91) - CLOSTRIDIUM CELLULOVORANS.

SCAN HISTORY OWL30_0 2 75 NSINGLE SPTR37_9f 2 9 NSINGLE INITIAL MOTIF SETS GLHYDRLASE481 Length of motif = 26 Motif number = 1 Glycosyl hydrolase family 48 motif I - 1 PCODE ST INT GIPYHSIETLIVEAPDYGHVTTSEAF GUNS_CLOTM 64 64 GIPYHAVETLIVEAPDYGHLTTSEAM GUX2_CLOSR 63 63 GIPYHSIETLMVEAPDYGHVTTSEAM GUNF_CLOCE 61 61 GIPYHSVETLICEAPDYGHLTTSEAF GUNA_CALSA 1140 1140 GIPYHAVETLMVEAPDYGHETTSEAY GUXB_CELFI 82 82 GIPYHSVETLICERPDYGHLTTSEAF ATZ86105 1109 1109 GLHYDRLASE482 Length of motif = 18 Motif number = 2 Glycosyl hydrolase family 48 motif II - 1 PCODE ST INT SYYVWLEAMYGNLTGNWS GUNS_CLOTM 90 0 SYYLWLEALYGKFTGDFS GUX2_CLOSR 89 0 SYYMWLEAMHGRFSGDFT GUNF_CLOCE 87 0 SYYVWLEAVYGKLTGDWS GUNA_CALSA 1166 0 SYWLWLEALYGQVTQDWA GUXB_CELFI 108 0 SYYVWLEAVYGKLTGDWS ATZ86105 1135 0 GLHYDRLASE483 Length of motif = 25 Motif number = 3 Glycosyl hydrolase family 48 motif III - 1 PCODE ST INT TFINTFQRGEQESTWETIPHPSIEE GUNS_CLOTM 201 93 AYINTFQRGSQESVWETIPQPCWDD GUX2_CLOSR 206 99 SYINTFQRGEQESTWETIPQPCWDE GUNF_CLOCE 204 99 SFINTFQRGPEESVWETVPHPSWEE GUNA_CALSA 1280 96 SFINTFQRGPQESVWETVPQPSCEE GUXB_CELFI 230 104 SFINTFQRGPEESVWETVPHPSWEE ATZ86105 1249 96 GLHYDRLASE484 Length of motif = 28 Motif number = 4 Glycosyl hydrolase family 48 motif IV - 1 PCODE ST INT KQWRYTNAPDAEGRAIQAVYWANKWAKE GUNS_CLOTM 246 20 AQFKYTNAPDADARAIQATYWANQWAKE GUX2_CLOSR 251 20 KQFKYTNAPDADARAVQATYWADQWAKE GUNF_CLOCE 250 21 KQWRYTNAPDADARAIQATYWAKVWAKE GUNA_CALSA 1325 20 KQWKYTSASDADARAVEAVYWANQWATE GUXB_CELFI 275 20 KQWRYTDAPDADARAIQATYWAKVWAKE ATZ86105 1294 20 GLHYDRLASE485 Length of motif = 20 Motif number = 5 Glycosyl hydrolase family 48 motif V - 1 PCODE ST INT TGYDSAHYLMAWYTAWGGGI GUNS_CLOTM 315 41 TGYDAAHYLLSWYYAWGGGI GUX2_CLOSR 317 38 TGYDAAHYLLSWYYAWGGGI GUNF_CLOCE 316 38 TGYDSAHYLLSWYYAWGGAL GUNA_CALSA 1396 43 QGREAAHYLLSWYMAWGGAT GUXB_CELFI 346 43 TGYDSAHYLLSWYYAWGGAL ATZ86105 1365 43 GLHYDRLASE486 Length of motif = 24 Motif number = 6 Glycosyl hydrolase family 48 motif VI - 1 PCODE ST INT RQLEFYQWLQSAEGGIAGGATNSW GUNS_CLOTM 383 48 RQLEFYQWLQSAEGAIAGGATNSY GUX2_CLOSR 385 48 RQLEFYQWLQSAEGAIAGGATNSW GUNF_CLOCE 384 48 RQIEFYRWLQSAEGAIAGGATNSW GUNA_CALSA 1464 48 RQLEFYTWLQASNGGIAGGATNSW GUXB_CELFI 416 50 RQIEFYRWLQSAEGAIAGGATNSW ATZ86105 1433 48 GLHYDRLASE487 Length of motif = 27 Motif number = 7 Glycosyl hydrolase family 48 motif VII - 1 PCODE ST INT PVYADPGSNQWFGFQAWSMQRVMEYYL GUNS_CLOTM 429 22 PVYLDPGSNTWFGFQAWTMQRVAEYYY GUX2_CLOSR 431 22 PVYADPGSNTWFGMQVWSMQRVAELYY GUNF_CLOCE 430 22 PVYRDPGSNTWFGFQAWSMQRVAEYYY GUNA_CALSA 1510 22 PVYVDPPSNRWFGMQAWGVQRVAELYY GUXB_CELFI 462 22 PVYHDPGSNTWFGFQAWSMQRVVEYYY ATZ86105 1479 22 GLHYDRLASE488 Length of motif = 21 Motif number = 8 Glycosyl hydrolase family 48 motif VIII - 1 PCODE ST INT TFAIPSDLEWSGQPDTWTGTY GUNS_CLOTM 484 28 TFEIPGNLEWSGQPDTWTGTY GUX2_CLOSR 486 28 TFQIPSTIDWEGQPDTWNPTQ GUNF_CLOCE 485 28 TFAIPSTLDWSGQPDTWNGTY GUNA_CALSA 1565 28 SWKVPSELKWTGKPDTWNAAA GUXB_CELFI 516 27 TFAIPSTLDWKRQPDTWNGAY ATZ86105 1534 28 GLHYDRLASE489 Length of motif = 29 Motif number = 9 Glycosyl hydrolase family 48 motif IX - 1 PCODE ST INT GWSGTMPNGDKIQPGIKFIDIRTKYRQDP GUNS_CLOTM 594 89 GWSGTMPNGDRIEPGVTFLDIRSKYLNDP GUX2_CLOSR 588 81 GWTGKMPNGDVIKSGVKFIDIRSKYKQDP GUNF_CLOCE 589 83 GWTGKMPNGDVIKSGVKFIDIRSKYKQDP GUNA_CALSA 1672 86 GWTGTMPNGDVIKPGVSFLDIRSFYKKDP GUXB_CELFI 625 88 GWIGKMPNGDVIKSGVKFIDIRSKYKQDP ATZ86105 1641 86 FINAL MOTIF SETS GLHYDRLASE481 Length of motif = 26 Motif number = 1 Glycosyl hydrolase family 48 motif I - 2 PCODE ST INT GIPYHSVETLMVEAPDYGHVTTSEAM O82831 61 61 GIPYHSVETLICEAPDYGHLTTSEAF GUNA_CALSA 1140 1140 GIPYHSIETLMVEAPDYGHVTTSEAM GUNF_CLOCE 61 61 GIPYHAVETLIVEAPDYGHLTTSEAM GUX2_CLOSR 63 63 GIPYHSIETLIVEAPDYGHVTTSEAF GUNS_CLOTM 64 64 GIPYHSVETLIVEAPDHGHETTSEAY O86728 367 367 GIPYHAVETLMVEAPDYGHETTSEAY GUXB_CELFI 82 82 GLHYDRLASE482 Length of motif = 18 Motif number = 2 Glycosyl hydrolase family 48 motif II - 2 PCODE ST INT SYYMWLEAMYGRFTGDFS O82831 87 0 SYYVWLEAVYGKLTGDWS GUNA_CALSA 1166 0 SYYMWLEAMHGRFSGDFT GUNF_CLOCE 87 0 SYYLWLEALYGKFTGDFS GUX2_CLOSR 89 0 SYYVWLEAMYGNLTGNWS GUNS_CLOTM 90 0 SYLLWLQAMYGKVTGDWS O86728 393 0 SYWLWLEALYGQVTQDWA GUXB_CELFI 108 0 GLHYDRLASE483 Length of motif = 25 Motif number = 3 Glycosyl hydrolase family 48 motif III - 2 PCODE ST INT SYINTFQRGEQESTWETIPQPCWDE O82831 204 99 SFINTFQRGPEESVWETVPHPSWEE GUNA_CALSA 1280 96 SYINTFQRGEQESTWETIPQPCWDE GUNF_CLOCE 204 99 AYINTFQRGSQESVWETIPQPCWDD GUX2_CLOSR 206 99 TFINTFQRGEQESTWETIPHPSIEE GUNS_CLOTM 201 93 SYINTFQRGAQESVWETVPQPTCDA O86728 514 103 SFINTFQRGPQESVWETVPQPSCEE GUXB_CELFI 230 104 GLHYDRLASE484 Length of motif = 28 Motif number = 4 Glycosyl hydrolase family 48 motif IV - 2 PCODE ST INT KQFKYTNAPDADARAVQATYWANEWAKE O82831 250 21 KQWRYTNAPDADARAIQATYWAKVWAKE GUNA_CALSA 1325 20 KQFKYTNAPDADARAVQATYWADQWAKE GUNF_CLOCE 250 21 AQFKYTNAPDADARAIQATYWANQWAKE GUX2_CLOSR 251 20 KQWRYTNAPDAEGRAIQAVYWANKWAKE GUNS_CLOTM 246 20 KQWKFTNAPDADARAVQAAYWADIWAGE O86728 559 20 KQWKYTSASDADARAVEAVYWANQWATE GUXB_CELFI 275 20 GLHYDRLASE485 Length of motif = 20 Motif number = 5 Glycosyl hydrolase family 48 motif V - 2 PCODE ST INT TGYDSAHYLLSWYYAWGGGV O82831 316 38 TGYDSAHYLLSWYYAWGGAL GUNA_CALSA 1396 43 TGYDAAHYLLSWYYAWGGGI GUNF_CLOCE 316 38 TGYDAAHYLLSWYYAWGGGI GUX2_CLOSR 317 38 TGYDSAHYLMAWYTAWGGGI GUNS_CLOTM 315 41 TGKDSSHYLLSWYYAWGGAV O86728 632 45 QGREAAHYLLSWYMAWGGAT GUXB_CELFI 346 43 GLHYDRLASE486 Length of motif = 24 Motif number = 6 Glycosyl hydrolase family 48 motif VI - 2 PCODE ST INT RQLEFYQWLQSSEGAIAGGATNSW O82831 384 48 RQIEFYRWLQSAEGAIAGGATNSW GUNA_CALSA 1464 48 RQLEFYQWLQSAEGAIAGGATNSW GUNF_CLOCE 384 48 RQLEFYQWLQSAEGAIAGGATNSY GUX2_CLOSR 385 48 RQLEFYQWLQSAEGGIAGGATNSW GUNS_CLOTM 383 48 RQVEFYRWLQSDEGAIAGGATNSW O86728 702 50 RQLEFYTWLQASNGGIAGGATNSW GUXB_CELFI 416 50 GLHYDRLASE487 Length of motif = 27 Motif number = 7 Glycosyl hydrolase family 48 motif VII - 2 PCODE ST INT PVYADPGSNTWFGMQVWSMQRVAELYY O82831 430 22 PVYRDPGSNTWFGFQAWSMQRVAEYYY GUNA_CALSA 1510 22 PVYADPGSNTWFGMQVWSMQRVAELYY GUNF_CLOCE 430 22 PVYLDPGSNTWFGFQAWTMQRVAEYYY GUX2_CLOSR 431 22 PVYADPGSNQWFGFQAWSMQRVMEYYL GUNS_CLOTM 429 22 PVYHDPPSNQWFGFQAWSMERVAEYYQ O86728 748 22 PVYVDPPSNRWFGMQAWGVQRVAELYY GUXB_CELFI 462 22 GLHYDRLASE488 Length of motif = 21 Motif number = 8 Glycosyl hydrolase family 48 motif VIII - 2 PCODE ST INT TFQIPGTLDWEGQPDTWDPTQ O82831 485 28 TFAIPSTLDWSGQPDTWNGTY GUNA_CALSA 1565 28 TFQIPSTIDWEGQPDTWNPTQ GUNF_CLOCE 485 28 TFEIPGNLEWSGQPDTWTGTY GUX2_CLOSR 486 28 TFAIPSDLEWSGQPDTWTGTY GUNS_CLOTM 484 28 TFRIPSTLQWSGQPDTWNASS O86728 803 28 SWKVPSELKWTGKPDTWNAAA GUXB_CELFI 516 27 GLHYDRLASE489 Length of motif = 29 Motif number = 9 Glycosyl hydrolase family 48 motif IX - 2 PCODE ST INT GWTGKMPNGDVIKSGVKFIDIRSKYKQDP O82831 589 83 GWTGKMPNGDVIKSGVKFIDIRSKYKQDP GUNA_CALSA 1672 86 GWTGKMPNGDVIKSGVKFIDIRSKYKQDP GUNF_CLOCE 589 83 GWSGTMPNGDRIEPGVTFLDIRSKYLNDP GUX2_CLOSR 588 81 GWSGTMPNGDKIQPGIKFIDIRTKYRQDP GUNS_CLOTM 594 89 GWSGTMPNGDTVDASSTFASIRSFYQDDP O86728 905 81 GWTGTMPNGDVIKPGVSFLDIRSFYKKDP GUXB_CELFI 625 88

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