WORKLIST ENTRIES (1):

GLHYDRLASE36 View alignment     Glycosyl hydrolase family 36 signature
 Type of fingerprint: COMPOUND with 8  elements
Links:
   PRINTS; PR00131 GLHYDRLASE1; PR00132 GLHYDRLASE2; PR00133 GLHYDRLASE3
   PRINTS; PR00732 GLHYDRLASE4; PR00733 GLHYDRLASE6; PR00734 GLHYDRLASE7
   PRINTS; PR00735 GLHYDRLASE8; PR00134 GLHYDRLASE10; PR00911 GLHYDRLASE11
   PRINTS; PR00736 GLHYDRLASE15; PR00737 GLHYDRLASE16; PR00738 GLHYDRLASE20
   PRINTS; PR00739 GLHYDRLASE26; PR00740 GLHYDRLASE27; PR00741 GLHYDRLASE29
   PRINTS; PR00843 GLHYDRLASE30; PR00742 GLHYDRLASE35; PR00744 GLHYDRLASE37
   PRINTS; PR00745 GLHYDRLASE39; PR00746 GLHYDRLASE41; PR00747 GLHYDRLASE47
   PRINTS; PR00844 GLHYDRLASE48; PR00845 GLHYDRLASE52; PR00846 GLHYDRLASE56
   PRINTS; PR00849 GLHYDRLASE58; PR00850 GLHYDRLASE59; PR00748 MELIBIASE
   PRINTS; PR00137 LYSOZYME; PR00684 T4LYSOZYME; PR00749 LYSOZYMEG
   PRINTS; PR00110 ALPHAAMYLASE; PR00750 BETAAMYLASE
   INTERPRO; IPR002252
   PROSITE; PS00512 ALPHA_GALACTOSIDASE

 Creation date 11-JUN-1997; UPDATE 07-JUN-1999

   1. HENRISSAT, B. AND BAIROCH, A.
   New families in the classification of glycosyl hydrolases based on amino
   acid sequence similarities.
   BIOCHEM.J. 293 781-788 (1993).

   2. HENRISSAT, B.
   A classification of glycosyl hydrolases based on amino acid sequence
   similarities.
   BIOCHEM.J. 280 309-316 (1991).

   3. DAVIES, G. AND HENRISSAT, B.
   Structures and mechanisms of glycosyl hydrolases.
   STRUCTURE 3 853-859 (1995).

   4. HENRISSAT, B. AND BAIROCH, A.
   Updating the sequence-based classification of glycosyl hydrolases.
   BIOCHEM.J. 316 695-696 (1996).

   5. TURAKAINEN, H., KRISTO, P. AND KORHOLA, M. 
   Consideration of the evolution of the Saccharomyces cerevisiae MEL gene
   family on the basis of the nucleotide sequences of the genes and their
   flanking regions.
   YEAST 10(12) 1559-1568 (1994). 

   6. ASLANIDIS C., SCHMID K., SCHMITT R.
   Nucleotide sequences and operon structure of plasmid-borne genes mediating
   uptake and utilization of raffinose in Escherichia coli.
   J.BACTERIOL. 171 6753-6763 (1989).

   O-Glycosyl hydrolases (EC 3.2.1.-) are a widespread group of enzymes that
   hydrolyse the glycosidic bond between two or more carbohydrates, or between
   a carbohydrate and a non-carbohydrate moiety. A classification system for
   glycosyl hydrolases, based on sequence similarity, has led to the definition
   of up to 60 different families [1-4] (http://expasy.hcuge.ch/cgi-bin/lists?
   glycosid.txt). Family 36 encompasses alpha-galactosidases.
   
   Alpha-galactosidase (melibiase) catalyses the hydrolysis of melibiose into
   galactose and glucose [5]. In man, deficiency in the enzyme results in
   Fabry's disease (X-linked sphingolipidosis). An E.coli plasmid-encoded
   alpha-galactosidase (rafA) [6] contains a region of about 50 amino acids
   that is similar to a domain of the eukaryotic alpha-galactosidases.
  
   GLHYDRLASE36 is an 8-element fingerprint that provides a signature for
   family 36 glycosyl hydrolases. The fingerprint was derived from an initial
   alignment of 4 sequences: the motifs were drawn from conserved regions 
   spanning the central portion of the alignment - motif 4 includes the
   region encoded by PROSITE pattern ALPHA_GALACTOSIDASE (PS00512), which
   contains two conserved Asp residues that may be involved in the catalytic
   mechanism. Two iterations on OWL29.3 were required to reach convergence, at
   which point a true set comprising 5 sequences was identified. A single 
   partial match was found, TEMELA, an alpha-galactosidase that matches motifs
   3 and 6.
  
   An update on SPTR37_9f identified a true set of 5 sequences.

  SUMMARY INFORMATION
      5 codes involving  8 elements
      0 codes involving  7 elements
      0 codes involving  6 elements
      0 codes involving  5 elements
      0 codes involving  4 elements
      0 codes involving  3 elements
      0 codes involving  2 elements

   COMPOSITE FINGERPRINT INDEX
  
    8|   5    5    5    5    5    5    5    5  
    7|   0    0    0    0    0    0    0    0  
    6|   0    0    0    0    0    0    0    0  
    5|   0    0    0    0    0    0    0    0  
    4|   0    0    0    0    0    0    0    0  
    3|   0    0    0    0    0    0    0    0  
    2|   0    0    0    0    0    0    0    0  
   --+-----------------------------------------
     |   1    2    3    4    5    6    7    8  

True positives..
 AGA1_PEDPE     AGAL_STRMU     RAFA_ECOLI     Q92457         
 AGA2_PEDPE     


  PROTEIN TITLES
   AGA1_PEDPE       ALPHA-GALACTOSIDASE 1 (EC 3.2.1.22) (MELIBIASE) - PEDIOCOCCU
   AGAL_STRMU       ALPHA-GALACTOSIDASE (EC 3.2.1.22) (MELIBIASE) - STREPTOCOCCU
   RAFA_ECOLI       ALPHA-GALACTOSIDASE (EC 3.2.1.22) (MELIBIASE) - ESCHERICHIA 
   Q92457           ALPHA-GALACTOSIDASE PRECURSOR (EC 3.2.1.22) (MELIBIASE) - TR
   AGA2_PEDPE       ALPHA-GALACTOSIDASE 2 (EC 3.2.1.22) (MELIBIASE) - PEDIOCOCCU

SCAN HISTORY OWL29_3 2 50 NSINGLE SPTR37_9f 2 6 NSINGLE INITIAL MOTIF SETS GLHYDRLASE361 Length of motif = 16 Motif number = 1 Glycosyl hydrolase family 36 motif I - 1 PCODE ST INT EFSGDAYGFNLVYSGN AGA1_PEDPE 243 243 EFYGEALALQLIYSGN AGAL_STRMU 235 235 EQQGEVWAVHLGWSGN RAFA_ECOLI 205 205 ELAGTVIGCALAWSGN AGA2_PEDPE 239 239 GLHYDRLASE362 Length of motif = 21 Motif number = 2 Glycosyl hydrolase family 36 motif II - 1 PCODE ST INT TPEVLMVYTNKGLNAMSQAYH AGA1_PEDPE 294 35 TPVALITYTDKGLTDLTQESH AGAL_STRMU 286 35 TPWLYACHSADGLNGMSQQYH RAFA_ECOLI 256 35 TPEAVLTWTNTGFNGMSQVFH AGA2_PEDPE 290 35 GLHYDRLASE363 Length of motif = 15 Motif number = 3 Glycosyl hydrolase family 36 motif III - 1 PCODE ST INT PIVVNNWEATFFDFN AGA1_PEDPE 332 17 PILINNWEATYFDFN AGAL_STRMU 324 17 PVHLNTWEGIYFNHN RAFA_ECOLI 293 16 PSVLNTWETLTFAVS AGA2_PEDPE 324 13 GLHYDRLASE364 Length of motif = 16 Motif number = 4 Glycosyl hydrolase family 36 motif IV - 1 PCODE ST INT LGIEMFVLDDGWFGHR AGA1_PEDPE 360 13 LGIELFVLDDGWFGHR AGAL_STRMU 352 13 LGVERFIIDDGWFKGR RAFA_ECOLI 321 13 LGLQMLVLDDGWFVNR AGA2_PEDPE 352 13 GLHYDRLASE365 Length of motif = 23 Motif number = 5 Glycosyl hydrolase family 36 motif V - 1 PCODE ST INT FGIWLEPEMISYDSKLYQQHPDY AGA1_PEDPE 410 34 FGLWFEPEMVSVDSKLYRAHPDW AGAL_STRMU 402 34 FGIWVEPEMINPDSDLFRLHPDW RAFA_ECOLI 371 34 FGLWVEPEMITTNSQLYQQHPDW AGA2_PEDPE 402 34 GLHYDRLASE366 Length of motif = 16 Motif number = 6 Glycosyl hydrolase family 36 motif VI - 1 PCODE ST INT LLKSKQIDYIKWDMNR AGA1_PEDPE 468 35 LLTENTIDYVKWDYNR AGAL_STRMU 460 35 LLGEHPVDYVKWDMNR RAFA_ECOLI 429 35 LVQNNQLDYLKWDMNR AGA2_PEDPE 460 35 GLHYDRLASE367 Length of motif = 17 Motif number = 7 Glycosyl hydrolase family 36 motif VII - 1 PCODE ST INT HILFEGCSGGGGRFDAG AGA1_PEDPE 522 38 KVLFESCSGGGGRNDLG AGAL_STRMU 513 37 HVEFESCASGGGRIDFE RAFA_ECOLI 477 32 KLIIENCSAGGGRFDFG AGA2_PEDPE 514 38 GLHYDRLASE368 Length of motif = 17 Motif number = 8 Glycosyl hydrolase family 36 motif VIII - 1 PCODE ST INT WASDNTDAVARLTIQYG AGA1_PEDPE 547 8 WSSDNTDAIARLPIQYG AGAL_STRMU 538 8 WASDNNDALERCTIQRG RAFA_ECOLI 502 8 WISDLTDPVDRATIENG AGA2_PEDPE 539 8 FINAL MOTIF SETS GLHYDRLASE361 Length of motif = 16 Motif number = 1 Glycosyl hydrolase family 36 motif I - 2 PCODE ST INT EFSGDAYGFNLVYSGN AGA1_PEDPE 243 243 EFYGEALALQLIYSGN AGAL_STRMU 235 235 EQQGEVWAVHLGWSGN RAFA_ECOLI 205 205 ESQGEAWGFSLVYTGS Q92457 257 257 ELAGTVIGCALAWSGN AGA2_PEDPE 239 239 GLHYDRLASE362 Length of motif = 21 Motif number = 2 Glycosyl hydrolase family 36 motif II - 2 PCODE ST INT TPEVLMVYTNKGLNAMSQAYH AGA1_PEDPE 294 35 TPVALITYTDKGLTDLTQESH AGAL_STRMU 286 35 TPWLYACHSADGLNGMSQQYH RAFA_ECOLI 256 35 SPEAVAVFSTTGVGGMSRKFH Q92457 308 35 TPEAVLTWTNTGFNGMSQVFH AGA2_PEDPE 290 35 GLHYDRLASE363 Length of motif = 15 Motif number = 3 Glycosyl hydrolase family 36 motif III - 2 PCODE ST INT PIVVNNWEATFFDFN AGA1_PEDPE 332 17 PILINNWEATYFDFN AGAL_STRMU 324 17 PVHLNTWEGIYFNHN RAFA_ECOLI 293 16 PVLLNSWEGLGFDYN Q92457 346 17 PSVLNTWETLTFAVS AGA2_PEDPE 324 13 GLHYDRLASE364 Length of motif = 16 Motif number = 4 Glycosyl hydrolase family 36 motif IV - 2 PCODE ST INT LGIEMFVLDDGWFGHR AGA1_PEDPE 360 13 LGIELFVLDDGWFGHR AGAL_STRMU 352 13 LGVERFIIDDGWFKGR RAFA_ECOLI 321 13 LGIKLFVLDDGWFGVK Q92457 374 13 LGLQMLVLDDGWFVNR AGA2_PEDPE 352 13 GLHYDRLASE365 Length of motif = 23 Motif number = 5 Glycosyl hydrolase family 36 motif V - 2 PCODE ST INT FGIWLEPEMISYDSKLYQQHPDY AGA1_PEDPE 410 34 FGLWFEPEMVSVDSKLYRAHPDW AGAL_STRMU 402 34 FGIWVEPEMINPDSDLFRLHPDW RAFA_ECOLI 371 34 FGLWFEPEMVNPNSTLYMEHPDW Q92457 434 44 FGLWVEPEMITTNSQLYQQHPDW AGA2_PEDPE 402 34 GLHYDRLASE366 Length of motif = 16 Motif number = 6 Glycosyl hydrolase family 36 motif VI - 2 PCODE ST INT LLKSKQIDYIKWDMNR AGA1_PEDPE 468 35 LLTENTIDYVKWDYNR AGAL_STRMU 460 35 LLGEHPVDYVKWDMNR RAFA_ECOLI 429 35 ILSNASISYVKWDNNR Q92457 492 35 LVQNNQLDYLKWDMNR AGA2_PEDPE 460 35 GLHYDRLASE367 Length of motif = 17 Motif number = 7 Glycosyl hydrolase family 36 motif VII - 2 PCODE ST INT HILFEGCSGGGGRFDAG AGA1_PEDPE 522 38 KVLFESCSGGGGRNDLG AGAL_STRMU 513 37 HVEFESCASGGGRIDFE RAFA_ECOLI 477 32 NVRWEGCASGGGRFDPG Q92457 538 30 KLIIENCSAGGGRFDFG AGA2_PEDPE 514 38 GLHYDRLASE368 Length of motif = 17 Motif number = 8 Glycosyl hydrolase family 36 motif VIII - 2 PCODE ST INT WASDNTDAVARLTIQYG AGA1_PEDPE 547 8 WSSDNTDAIARLPIQYG AGAL_STRMU 538 8 WASDNNDALERCTIQRG RAFA_ECOLI 502 8 WTSDDTDAVERIAIQFG Q92457 563 8 WISDLTDPVDRATIENG AGA2_PEDPE 539 8

User query: Display/Full Code "GLHYDRLASE36"