WORKLIST ENTRIES (1):

GLHYDRLASE30 View alignment     Glycosyl hydrolase family 30 signature
 Type of fingerprint: COMPOUND with 6  elements
Links:
   PRINTS; PR00131 GLHYDRLASE1; PR00132 GLHYDRLASE2; PR00133 GLHYDRLASE3
   PRINTS; PR00732 GLHYDRLASE4; PR00733 GLHYDRLASE6; PR00734 GLHYDRLASE7
   PRINTS; PR00735 GLHYDRLASE8; PR00134 GLHYDRLASE10; PR00911 GLHYDRLASE11
   PRINTS; PR00736 GLHYDRLASE15; PR00737 GLHYDRLASE16; PR00738 GLHYDRLASE20
   PRINTS; PR00739 GLHYDRLASE26; PR00740 GLHYDRLASE27; PR00741 GLHYDRLASE29
   PRINTS; PR00742 GLHYDRLASE35; PR00743 GLHYDRLASE36; PR00744 GLHYDRLASE37
   PRINTS; PR00745 GLHYDRLASE39; PR00746 GLHYDRLASE41; PR00747 GLHYDRLASE47
   PRINTS; PR00844 GLHYDRLASE48; PR00845 GLHYDRLASE52; PR00846 GLHYDRLASE56
   PRINTS; PR00849 GLHYDRLASE58; PR00850 GLHYDRLASE59; PR00748 MELIBIASE
   PRINTS; PR00137 LYSOZYME; PR00684 T4LYSOZYME; PR00749 LYSOZYMEG
   PRINTS; PR00110 ALPHAAMYLASE; PR00750 BETAAMYLASE
   INTERPRO; IPR001139

 Creation date 13-FEB-1998; UPDATE 07-JUN-1999

   1. HENRISSAT, B.
   A classification of glycosyl hydrolases based on amino acid sequence
   similarities.
   BIOCHEM.J. 280 309-316 (1991).

   2. HENRISSAT, B. AND BAIROCH, A.
   New families in the classification of glycosyl hydrolases based on amino
   acid sequence similarities.
   BIOCHEM.J. 293 781-788 (1993).

   3. HENRISSAT, B. AND BAIROCH, A.
   Updating the sequence-based classification of glycosyl hydrolases.
   BIOCHEM.J. 316 695-696 (1996).

   4. EL HASSOUNI, M., HENRISSAT, B., CHIPPAUX, M. AND BARRAS, F.
   Nucleotide sequences of the Arb genes, which control beta-glucosidase
   utilisation in Erwinia chrysanthemi - Comparison with the Escherichia
   coli Bgl operon and evidence for a new beta-glycohydrolase family
   including enzymes from eubacteria, archaebacteria and humans.
   J.BACTERIOL. 174 765-777 (1992).
  
   5. DINUR, T., OSIECKI, K.M., LEGLER, G., GATT, S., DESNICK, R.J.
   AND GRABOWSKI, G.A.
   Human acid beta-glucosidase: isolation and amino acid sequence of a peptide
   containing the catalytic site. 
   PROC.NATL.ACAD.SCI.U.S.A. 83 1660-1664 (1986). 

   6. WINFIELD, S.L., TAYEBI, N., MARTIN, B.M., GINNS, E.I. AND SIDRANSKY, E.
   Identification of three additional genes contiguous to the glucocerebrosidase 
   locus on chromosome 1q21: Implications for Gaucher disease.
   GENOME RES. 7 1020-1026 (1997).

   7. IWASAWA, K., IDA, H. AND ETO, Y.
   Differences in origin of the 1448C mutation in patients with Gaucher disease.
   ACTA PAEDIATR.JPN. 39 451-453 (1997).

   O-Glycosyl hydrolases (EC 3.2.1.-) are a widespread group of enzymes that
   hydrolyse the glycosidic bond between two or more carbohydrates, or between
   a carbohydrate and a non-carbohydrate moiety. A classification system for
   glycosyl hydrolases, based on sequence similarity, has led to the definition
   of up to 60 different families [1-4] (http://expasy.hcuge.ch/cgi-bin/lists?
   glycosid.txt). Family 30 encompasses the mammalian glucosyl-ceramidases (EC
   3.2.1.45).
  
   Human acid beta-glucosidase (D-glucosyl-N-acylsphingosine glucohydrolase),
   cleaves the glucosidic bonds of glucosylceramide and synthetic beta-
   glucosides [5]. Any one of over 50 different mutations in the gene of
   glucocerebrosidase have been found to affect activity of this hydrolase,
   producing variants of Gaucher disease, the most prevalent lysosomal
   storage disease [5,7]. 
  
   GLHYDRLASE30 is a 6-element fingerprint that provides a signature for
   family 30 glycosyl hydrolases. The fingerprint was derived from an initial
   alignment of 5 sequences: the motifs were drawn from conserved regions 
   spanning the N-terminal half of the alignment - motifs 1 and 4 each include
   potential glycosylation sites; and motif 5 encodes a putative proton donor
   site. A single iteration on OWL29.6 was required to reach convergence, no
   further sequences being identified beyond the starting set. Two partial
   matches were found: CEF11E6 is a fragment that lacks the portion of sequence
   bearing motifs 5 and 6; and I67792, a putative glucosylceramidase that
   matches motifs 4, 5 and 6.
  
   An update on SPTR37_9f identified a true set of 5 sequences.

  SUMMARY INFORMATION
      5 codes involving  6 elements
      0 codes involving  5 elements
      0 codes involving  4 elements
      0 codes involving  3 elements
      0 codes involving  2 elements

   COMPOSITE FINGERPRINT INDEX
  
    6|   5    5    5    5    5    5  
    5|   0    0    0    0    0    0  
    4|   0    0    0    0    0    0  
    3|   0    0    0    0    0    0  
    2|   0    0    0    0    0    0  
   --+-------------------------------
     |   1    2    3    4    5    6  

True positives..
 GLCM_HUMAN     Q16545         GLCM_MOUSE     O16580         
 O16581         


  PROTEIN TITLES
   GLCM_HUMAN       GLUCOSYLCERAMIDASE PRECURSOR (EC 3.2.1.45) (BETA-GLUCOCEREBR
   Q16545           GLUCOCEREBROSIDASE PRECURSOR - HOMO SAPIENS (HUMAN).
   GLCM_MOUSE       GLUCOSYLCERAMIDASE PRECURSOR (EC 3.2.1.45) (BETA-GLUCOCEREBR
   O16580           C33C12.3 PROTEIN - CAENORHABDITIS ELEGANS.
   O16581           C33C12.8 PROTEIN - CAENORHABDITIS ELEGANS.

SCAN HISTORY OWL29_6 1 50 NSINGLE SPTR37_9f 2 6 NSINGLE INITIAL MOTIF SETS GLHYDRLASE301 Length of motif = 20 Motif number = 1 Glycosyl hydrolase family 30 motif I - 1 PCODE ST INT SSVVCVCNATYCDSFDPPTF GLCM_HUMAN 51 51 SSVVCVCNATYCDSFDPPTF HUMGCBL 31 31 SSVVCVCNASYCDSLDPVTL GLCM_MOUSE 31 31 TGIVCVCNITYCDEIPDINL CELC33C125 78 78 TGTVCVCSLDSCDEIPPLDI CELC33C124 33 33 GLHYDRLASE302 Length of motif = 21 Motif number = 2 Glycosyl hydrolase family 30 motif II - 1 PCODE ST INT TLQPEQKFQKVKGFGGAMTDA GLCM_HUMAN 107 36 TLQPEQKFQKVKGFGGAMTDA HUMGCBL 87 36 TLQPEKKFQKVKGFGGAMTDA GLCM_MOUSE 87 36 TIDSSKTYQTIQGFGSTFSDA CELC33C125 133 35 TIDSSKKYQTIQGFGSTFSDA CELC33C124 88 35 GLHYDRLASE303 Length of motif = 27 Motif number = 3 Glycosyl hydrolase family 30 motif III - 1 PCODE ST INT LLLKSYFSEEGIGYNIIRVPMASCDFS GLCM_HUMAN 142 14 LLLKSYFSEEGIGYNIIRVPMASCDFS HUMGCBL 122 14 LLLRSYFSTNGIEYNIIRVPMASCDFS GLCM_MOUSE 122 14 TILRQYFSDSGLNLQFGRVPIASNDFS CELC33C125 168 14 LIMKQYFSDTGLNLQFGRVPIASTDFS CELC33C124 123 14 GLHYDRLASE304 Length of motif = 29 Motif number = 4 Glycosyl hydrolase family 30 motif IV - 1 PCODE ST INT RTYTYADTPDDFQLHNFSLPEEDTKLKIP GLCM_HUMAN 170 1 RTYTYADTPDDFQLHNFSLPEEDTKLKIP HUMGCBL 150 1 RVYTYADTPNDFQLSNFSLPEEDTKLKIP GLCM_MOUSE 150 1 RVYTYDDNLEDYNMAHFSLQREDYQWKIP CELC33C125 196 1 RVYSYNDVANDYSMQNFNLTKEDFQWKIP CELC33C124 151 1 GLHYDRLASE305 Length of motif = 18 Motif number = 5 Glycosyl hydrolase family 30 motif V - 1 PCODE ST INT DIYHQTWARYFVKFLDAY GLCM_HUMAN 242 43 DIYHQTWARYFVKFLDAY HUMGCBL 222 43 DIFHQTWANYFVKFLDAY GLCM_MOUSE 222 43 DTYHKSYVTYILHFLEEY CELC33C125 267 42 DNYHQAYAKYFVRFLEEY CELC33C124 222 42 GLHYDRLASE306 Length of motif = 23 Motif number = 6 Glycosyl hydrolase family 30 motif VI - 1 PCODE ST INT VRLLMLDDQRLLLPHWAKVVLTD GLCM_HUMAN 315 55 VRLLMLDDQRLLLPHWAKVVLTD HUMGCBL 295 55 VKLLMLDDQRLLLPRWAEVVLSD GLCM_MOUSE 294 54 VKILILDDNRGNLPKWADTVLND CELC33C125 341 56 VKLLILDDNRGNLPKWADTVLND CELC33C124 296 56 FINAL MOTIF SETS GLHYDRLASE301 Length of motif = 20 Motif number = 1 Glycosyl hydrolase family 30 motif I - 2 PCODE ST INT SSVVCVCNATYCDSFDPPTF GLCM_HUMAN 51 51 SSVVCVCNATYCDSFDPPTF Q16545 51 51 SSVVCVCNASYCDSLDPVTL GLCM_MOUSE 31 31 TGTVCVCSLDSCDEIPPLDI O16580 33 33 TGIVCVCNITYCDEIPDINL O16581 78 78 GLHYDRLASE302 Length of motif = 21 Motif number = 2 Glycosyl hydrolase family 30 motif II - 2 PCODE ST INT TLQPEQKFQKVKGFGGAMTDA GLCM_HUMAN 107 36 TLQPEQKFQKVKGFGGAMTDA Q16545 107 36 TLQPEKKFQKVKGFGGAMTDA GLCM_MOUSE 87 36 TIDSSKKYQTIQGFGSTFSDA O16580 88 35 TIDSSKTYQTIQGFGSTFSDA O16581 133 35 GLHYDRLASE303 Length of motif = 27 Motif number = 3 Glycosyl hydrolase family 30 motif III - 2 PCODE ST INT LLLKSYFSEEGIGYNIIRVPMASCDFS GLCM_HUMAN 142 14 LLLKSYFSEEGIGYNIIRVPMASCDFS Q16545 142 14 LLLRSYFSTNGIEYNIIRVPMASCDFS GLCM_MOUSE 122 14 LIMKQYFSDTGLNLQFGRVPIASTDFS O16580 123 14 TILRQYFSDSGLNLQFGRVPIASNDFS O16581 168 14 GLHYDRLASE304 Length of motif = 29 Motif number = 4 Glycosyl hydrolase family 30 motif IV - 2 PCODE ST INT RTYTYADTPDDFQLHNFSLPEEDTKLKIP GLCM_HUMAN 170 1 RTYTYADTPDDFQLHNFSLPEEDTKLKIP Q16545 170 1 RVYTYADTPNDFQLSNFSLPEEDTKLKIP GLCM_MOUSE 150 1 RVYSYNDVANDYSMQNFNLTKEDFQWKIP O16580 151 1 RVYTYDDNLEDYNMAHFSLQREDYQWKIP O16581 196 1 GLHYDRLASE305 Length of motif = 18 Motif number = 5 Glycosyl hydrolase family 30 motif V - 2 PCODE ST INT DIYHQTWARYFVKFLDAY GLCM_HUMAN 242 43 DIYHQTWARYFVKFLDAY Q16545 242 43 DIFHQTWANYFVKFLDAY GLCM_MOUSE 222 43 DNYHQAYAKYFVRFLEEY O16580 222 42 DTYHKSYVTYILHFLEEY O16581 267 42 GLHYDRLASE306 Length of motif = 23 Motif number = 6 Glycosyl hydrolase family 30 motif VI - 2 PCODE ST INT VRLLMLDDQRLLLPHWAKVVLTD GLCM_HUMAN 315 55 VRLLMLDDQRLLLPHWAKVVLTD Q16545 315 55 VKLLMLDDQRLLLPRWAEVVLSD GLCM_MOUSE 294 54 VKLLILDDNRGNLPKWADTVLND O16580 296 56 VKILILDDNRGNLPKWADTVLND O16581 341 56

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