WORKLIST ENTRIES (1):

GLABLOOD View alignment         Coagulation factor GLA domain signature
 Type of fingerprint: COMPOUND with 3  elements
Links:
   PRINTS; PR00002 GLABONE
   INTERPRO; IPR002383
   PROSITE; PS00011 GLU_CARBOXYLATION
   PFAM; PF00594 gla
   PDB; 1APO 3Dinfo; 2PF2 3Dinfo
   SCOP; 1APO; 2PF2
   CATH; 1APO; 2PF2

 Creation date 12-JUL-1991; UPDATE 27-JUN-1999

   1. SORIANO-GARCIA, M., CHANG, H.P., TULINSKY, A., RAVICHANDRAN, K.G.
   AND SKRZYPCZAK-JANKUN, E.
   Structure of calcium prothrombin fragment 1 including the conformation
   of the Gla domain.
   BIOCHEMISTRY 28 6805-6810 (1989).
   
   2. CHURCH, W.R., MESSIER, T., HOWARD, P.R., AMIRAL, J., MEYER, D. 
   AND MANN, K.G.
   A conserved epitope on several human vitamin K-dependent proteins.
   J.BIOL.CHEM. 263 6259-6267 (1988).

   3. FRIEZNER DEGEN, S.J. AND DAVIE, E.W.
   Nucleotide sequence of the gene for human prothrombin.
   BIOCHEMISTRY 26 6165-6177 (1987).

   Coagulation proteins, such as factors VII, IX and X, proteins C and S, and 
   prothrombin, contain gamma-carboxyglutamic acid residues: these vary in 
   number between 9 and 12, and are produced by post-translational 
   modification of Glu residues by a vitamin K-dependent carboxylase. Calcium 
   ions bind to the gamma-carboxyglutamic acid residues of the Gla domain and 
   support the binding of these vitamin K-dependent proteins to acidic 
   phospholipid vesicles, resulting in the production of a soluble fibrin clot.
   
   The Gla domain is an N-terminal module of about 48 residues that contains
   carboxylated Glu residues responsible for high-affinity calcium binding.
   It terminates with a conserved aromatic residue, and at its centre has a
   conserved Gla-x(3)-Gla-x-Cys motif, which seems to be important for the
   substrate recognition by the carboxylase. The domain is dominated by
   secondary structure comprising 3 turns of alpha-helix and 5 short beta-
   strands arranged into 2 beta-structural units. Cys residues 18 and 23 of
   the Gla domain participate in a disulphide bridge to form a small conserved
   loop close to a cluster of conserved aromatic residues.
   
   GLABLOOD is a 3-element fingerprint that provides a signature for the N-
   terminal 'Gla domain' of coagulation proteins. The fingerprint was derived
   from an initial alignment of 6 sequences (after Soriano-Garcia [1]): the
   motifs span the region encoded by PROSITE pattern GLU_CARBOXYLATION
   (PS00011), 1 and 2 encoding the Gla-x(3)-Gla-x-Cys motif. Four iterations
   on OWL11.0 were required to reach convergence, at which point a true set
   comprising 29 sequences was identified. A single partial match was also
   found, a coagulation factor fragment, which lacks part of motif 3. 
  
   An update on SPTR37_9f identified a true set of 38 sequences, and 1
   partial match.

  SUMMARY INFORMATION
     38 codes involving  3 elements
      1 codes involving  2 elements

   COMPOSITE FINGERPRINT INDEX
  
    3|  38   38   38  
    2|   0    1    1  
   --+----------------
     |   1    2    3  

True positives..
 Q63207         FA10_BOVIN     FA10_RABIT     FA9_HUMAN      
 FA9_CANFA      FA10_HUMAN     FA9_BOVIN      PRTS_RAT       
 PRTS_HUMAN     Q16519         FA7_RABIT      FA10_CHICK     
 FA7_HUMAN      Q14339         PRTS_BOVIN     O54740         
 O88947         PRTS_MOUSE     Q14393         THRB_MOUSE     
 THRB_RAT       FA7_MOUSE      Q61109         PRTC_MOUSE     
 PRTC_RAT       Q15213         PRTZ_HUMAN     THRB_HUMAN     
 PRTZ_BOVIN     Q63772         FA7_BOVIN      O14668         
 THRB_BOVIN     Q91001         Q61592         O14669         
 PRTC_HUMAN     Q17343         


  PROTEIN TITLES
   Q63207           FACTOR X - RATTUS NORVEGICUS (RAT).
   FA10_BOVIN       COAGULATION FACTOR X PRECURSOR (EC 3.4.21.6) (STUART FACTOR)
   FA10_RABIT       COAGULATION FACTOR X PRECURSOR (EC 3.4.21.6) (STUART FACTOR)
   FA9_HUMAN        COAGULATION FACTOR IX PRECURSOR (EC 3.4.21.22) (CHRISTMAS FA
   FA9_CANFA        COAGULATION FACTOR IX PRECURSOR (EC 3.4.21.22) (CHRISTMAS FA
   FA10_HUMAN       COAGULATION FACTOR X PRECURSOR (EC 3.4.21.6) (STUART FACTOR)
   FA9_BOVIN        COAGULATION FACTOR IX (EC 3.4.21.22) (CHRISTMAS FACTOR) - BO
   PRTS_RAT         VITAMIN K-DEPENDENT PROTEIN S PRECURSOR - RATTUS NORVEGICUS 
   PRTS_HUMAN       VITAMIN K-DEPENDENT PROTEIN S PRECURSOR - HOMO SAPIENS (HUMA
   Q16519           PROTEIN S PRECURSOR - HOMO SAPIENS (HUMAN).
   FA7_RABIT        COAGULATION FACTOR VII PRECURSOR (EC 3.4.21.21) - ORYCTOLAGU
   FA10_CHICK       COAGULATION FACTOR X PRECURSOR (EC 3.4.21.6) (STUART FACTOR)
   FA7_HUMAN        COAGULATION FACTOR VII PRECURSOR (EC 3.4.21.21) (EPTACOG ALF
   Q14339           PREPROFACTOR VIIB PRECURSOR - HOMO SAPIENS (HUMAN).
   PRTS_BOVIN       VITAMIN K-DEPENDENT PROTEIN S PRECURSOR - BOS TAURUS (BOVINE
   O54740           COAGULATION FACTOR X PRECURSOR (EC 3.4.21.6) - MUS MUSCULUS 
   O88947           COAGULATION FACTOR X PRECURSOR - MUS MUSCULUS (MOUSE).
   PRTS_MOUSE       VITAMIN K-DEPENDENT PROTEIN S PRECURSOR - MUS MUSCULUS (MOUS
   Q14393           GROWTH-ARREST-SPECIFIC PROTEIN - HOMO SAPIENS (HUMAN).
   THRB_MOUSE       PROTHROMBIN PRECURSOR (EC 3.4.21.5) - MUS MUSCULUS (MOUSE).
   THRB_RAT         PROTHROMBIN PRECURSOR (EC 3.4.21.5) - RATTUS NORVEGICUS (RAT
   FA7_MOUSE        COAGULATION FACTOR VII PRECURSOR (EC 3.4.21.21) - MUS MUSCUL
   Q61109           COAGULATION FACTOR VII - MUS MUSCULUS (MOUSE).
   PRTC_MOUSE       VITAMIN-K DEPENDENT PROTEIN C PRECURSOR (EC 3.4.21.69) (AUTO
   PRTC_RAT         VITAMIN-K DEPENDENT PROTEIN C PRECURSOR (EC 3.4.21.69) (AUTO
   Q15213           PROTEIN Z PRECURSOR - HOMO SAPIENS (HUMAN).
   PRTZ_HUMAN       VITAMIN K-DEPENDENT PROTEIN Z PRECURSOR - HOMO SAPIENS (HUMA
   THRB_HUMAN       PROTHROMBIN PRECURSOR (EC 3.4.21.5) (COAGULATION FACTOR II) 
   PRTZ_BOVIN       VITAMIN K-DEPENDENT PROTEIN Z - BOS TAURUS (BOVINE).
   Q63772           GROWTH POTENTIATING FACTOR PRECURSOR - RATTUS NORVEGICUS (RA
   FA7_BOVIN        COAGULATION FACTOR VII (EC 3.4.21.21) - BOS TAURUS (BOVINE).
   O14668           PROLINE-RICH GLA PROTEIN 1 - HOMO SAPIENS (HUMAN).
   THRB_BOVIN       PROTHROMBIN PRECURSOR (EC 3.4.21.5) - BOS TAURUS (BOVINE).
   Q91001           THROMBIN - GALLUS GALLUS (CHICKEN).
   Q61592           GAS 6 MRNA ASSOCIATED WITH GROWTH-ARREST - MUS MUSCULUS (MOU
   O14669           PROLINE-RICH GLA PROTEIN 2 - HOMO SAPIENS (HUMAN).
   PRTC_HUMAN       VITAMIN-K DEPENDENT PROTEIN C PRECURSOR (EC 3.4.21.69) (AUTO
   Q17343           UNC-44 ANKYRINS - CAENORHABDITIS ELEGANS.

SCAN HISTORY OWL11_0 4 50 NSINGLE OWL17_1 1 70 NSINGLE OWL18_0 1 50 NSINGLE OWL19_1 1 50 NSINGLE OWL26_0 1 100 NSINGLE SPTR37_9f 2 100 NSINGLE INITIAL MOTIF SETS GLABLOOD1 Length of motif = 14 Motif number = 1 Coagulation factor Gla domain motif I - 1 PCODE ST INT FLEEVRKGNLEREC EZEC545 5 5 FLEEVRKGNLEREC EZEC546 4 4 FLEELRPGSLEREC FA7_HUMAN 64 64 KLEEFVQGNLEREC KFHU 51 51 FLEEVRKGNLEREC TBHU 40 40 FLEEMKKGHLEREC EXHU 32 32 GLABLOOD2 Length of motif = 14 Motif number = 2 Coagulation factor Gla domain motif II - 1 PCODE ST INT LEEPCSREEAFEAL EZEC545 19 0 VEETCSYEEAFEAL EZEC546 18 0 KEEQCSFEEAREIF FA7_HUMAN 78 0 MEEKCSFEEAREVF KFHU 65 0 VEETCSYEEAFEAL TBHU 54 0 MEETCSYEEAREVF EXHU 46 0 GLABLOOD3 Length of motif = 15 Motif number = 3 Coagulation factor Gla domain motif III - 1 PCODE ST INT ESLSATDAFWAKYTA EZEC545 33 0 ESSTATDVFWAKYTA EZEC546 32 0 KDAERTKLFWISYSD FA7_HUMAN 92 0 ENTERTTEFWKQYVD KFHU 79 0 ESSTATDVFWAKYTA TBHU 68 0 EDSDKTNEFWNKYKD EXHU 60 0 FINAL MOTIF SETS GLABLOOD1 Length of motif = 14 Motif number = 1 Coagulation factor Gla domain motif I - 2 PCODE ST INT FFEEIKKGNLEREC Q63207 44 44 FLEEVKQGNLEREC FA10_BOVIN 44 44 FLEELKKGNLEREC FA10_RABIT 44 44 KLEEFVQGNLEREC FA9_HUMAN 51 51 KLEEFVRGNLEREC FA9_CANFA 44 44 FLEEMKKGHLEREC FA10_HUMAN 44 44 KLEEFVRGNLEREC FA9_BOVIN 5 5 LLEETKKGNLEREC PRTS_RAT 45 45 LLEETKQGNLEREC PRTS_HUMAN 45 45 LLEETKQGNLEREC Q16519 19 19 FLEELRPGSLEREC FA7_RABIT 43 43 FLEEMKQGNIEREC FA10_CHICK 44 44 FLEELRPGSLEREC FA7_HUMAN 64 64 FLEELRPGSLEREC Q14339 42 42 LLEETKKGNLEREC PRTS_BOVIN 45 45 FFEEFKKGNLEREC O54740 44 44 FFEEFKKGNLEREC O88947 44 44 LFEETMKGNLEREC PRTS_MOUSE 45 45 VFEEAKQGHLEREC Q14393 52 52 FLEELRKGNLEREC THRB_MOUSE 48 48 FLEELRKGNLEREC THRB_RAT 48 48 LLEELWPGSLEREC FA7_MOUSE 45 45 LLEELWPGSLEREC Q61109 45 45 FLEEMRPGSLEREC PRTC_MOUSE 45 45 FLEEVRAGSLEREC PRTC_RAT 45 45 LLEELFEGNLEKEC Q15213 67 67 LLEELFEGNLEKEC PRTZ_HUMAN 45 45 FLEEVRKGNLEREC THRB_HUMAN 47 47 LLEELFEGHLEKEC PRTZ_BOVIN 5 5 VFEEAKQGHLEREC Q63772 49 49 FLEELLPGSLEREC FA7_BOVIN 4 4 FFEEIRQGNIEREC O14668 24 24 FLEEVRKGNLEREC THRB_BOVIN 48 48 FLEEMIKGNLEREC Q91001 48 48 VFEEAKQGHLEREC Q61592 49 49 DLELLTPGNLEREC O14669 54 54 FLEELRHSSLEREC PRTC_HUMAN 46 46 FLRAARAGDLEKVL Q17343 37 37 GLABLOOD2 Length of motif = 14 Motif number = 2 Coagulation factor Gla domain motif II - 2 PCODE ST INT VEEICSFEEAREVF Q63207 58 0 LEEACSLEEAREVF FA10_BOVIN 58 0 MEENCSYEEALEVF FA10_RABIT 58 0 MEEKCSFEEAREVF FA9_HUMAN 65 0 IEEKCSFEEAREVF FA9_CANFA 58 0 MEETCSYEEAREVF FA10_HUMAN 58 0 KEEKCSFEEAREVF FA9_BOVIN 19 0 IEELCNKEEAREVF PRTS_RAT 59 0 IEELCNKEEAREVF PRTS_HUMAN 59 0 IEELCNKEEAREVF Q16519 33 0 KEELCSFEEAREVF FA7_RABIT 57 0 NEERCSKEEAREAF FA10_CHICK 58 0 KEEQCSFEEAREIF FA7_HUMAN 78 0 KEEQCSFEEAREIF Q14339 56 0 IEELCNKEEAREIF PRTS_BOVIN 59 0 MEEICSYEEVREIF O54740 58 0 MEEICSYEEVREIF O88947 58 0 IEELCNKEEAREVF PRTS_MOUSE 59 0 VEELCSREEAREVF Q14393 66 0 VEEQCSYEEAFEAL THRB_MOUSE 62 0 VEEQCSYEEAFEAL THRB_RAT 62 0 NEEQCSFEEAREIF FA7_MOUSE 59 0 NEEQCSFEEAREIF Q61109 59 0 MEEICDFEEAQEIF PRTC_MOUSE 59 0 MEEICDFEEAQEIF PRTC_RAT 59 0 YEEICVYEEAREVF Q15213 81 0 YEEICVYEEAREVF PRTZ_HUMAN 59 0 VEETCSYEEAFEAL THRB_HUMAN 61 0 WEEICVYEEAREVF PRTZ_BOVIN 19 0 VEEVCSKEEAREVF Q63772 63 0 REELCSFEEAHEIF FA7_BOVIN 18 0 KEEFCTFEEAREAF O14668 38 0 LEEPCSREEAFEAL THRB_BOVIN 62 0 LEETCNYEEAFEAL Q91001 62 0 VEEVCSKEEAREVF Q61592 63 0 LEERCSWEEAREYF O14669 68 0 IEEICDFEEAKEIF PRTC_HUMAN 60 0 DEEKLEGHEEKEVL Q17343 2260 2209 GLABLOOD3 Length of motif = 15 Motif number = 3 Coagulation factor Gla domain motif III - 2 PCODE ST INT EDNEKTTEFWNKYED Q63207 72 0 EDAEQTDEFWSKYKD FA10_BOVIN 72 0 EDREKTNEFWNKYVD FA10_RABIT 72 0 ENTERTTEFWKQYVD FA9_HUMAN 79 0 ENTEKTTEFWKQYVD FA9_CANFA 72 0 EDSDKTNEFWNKYKD FA10_HUMAN 72 0 ENTEKTTEFWKQYVD FA9_BOVIN 33 0 ENNPETDYFYPKYLG PRTS_RAT 73 0 ENDPETDYFYPKYLV PRTS_HUMAN 73 0 ENDPETDYFYPKYLV Q16519 47 0 QSTERTKQFWITYND FA7_RABIT 71 0 EDNEKTEEFWNIYVD FA10_CHICK 72 0 KDAERTKLFWISYSD FA7_HUMAN 92 0 KDAERTKLFWISYSD Q14339 70 0 ENNPETEYFYPKYLG PRTS_BOVIN 73 0 EDDEKTKEYWTKYKD O54740 72 0 EDDEKTKEYWTKYKD O88947 72 0 ENNPETDYFYPKYLG PRTS_MOUSE 73 0 ENDPETDYFYPRYLD Q14393 80 0 ESPQDTDVFWAKYTV THRB_MOUSE 76 0 ESPQDTDVFWAKYTV THRB_RAT 76 0 KSPERTKQFWIVYSD FA7_MOUSE 73 0 KSPERTKQFWIVYSD Q61109 73 0 QNVEDTLAFWIKYFD PRTC_MOUSE 73 0 QNVEDTLAFWIKYFD PRTC_RAT 73 0 ENEVVTDEFWRRYKG Q15213 95 0 ENEVVTDEFWRRYKG PRTZ_HUMAN 73 0 ESSTATDVFWAKYTA THRB_HUMAN 75 0 EDDETTDEFWRTYMG PRTZ_BOVIN 33 0 ENDPETDYFYPRYQE Q63772 77 0 RNEERTRQFWVSYND FA7_BOVIN 32 0 ENNEKTKEFWSTYTK O14668 52 0 ESLSATDAFWAKYTA THRB_BOVIN 76 0 ESTVDTDAFWAKYQV Q91001 76 0 ENDPETEYFYPRYQE Q61592 77 0 EDNTLTERFWESYIY O14669 82 0 QNVDDTLAFWSKHVD PRTC_HUMAN 74 0 TSTTETREFDRPELD Q17343 3040 766

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